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The map label for this gene is potD [H]

Identifier: 77465516

GI number: 77465516

Start: 594499

End: 595536

Strand: Direct

Name: potD [H]

Synonym: RSP_3515

Alternate gene names: 77465516

Gene position: 594499-595536 (Clockwise)

Preceding gene: 77465514

Following gene: 77465517

Centisome position: 63.04

GC content: 68.88

Gene sequence:

>1038_bases
ATGATGGATCGGGTTTGCAGGCTGTCGCTCGCGGCGCTGCTCGCGGGTTCCGGCGCCGCCTACGCGGACGGCTCGACGCT
GACCGTCGCCTACTACGGCGGCAACTGGGGCGAGGCCTTCGACGCCTGCGTGGCGCAGCCCTTCACCGAGAAGACGGGCA
TCCGGGTGGTGGCCGAGATCGGCAACTCGACCACCACGCTCGCCAAGCTGCAGCAGCAGTCGGGCGATGCGGTGATCGAC
GTGGCCTATATGGACGGCGGGATCAGCGAGCTCGCCCAAGAGGCGGGCGTGCTGGCCCCCATCGATCTCGCCGCGATCCC
GAACGCGGCCAGCTACCTGCCGCAGGCCGTCTACAAGGCGGGCGACGAGGTCTTCGCGGTCAGCGCGGGCTATTACTCGC
TCGGCCTCATCTACAACACGTCGGAAGTGACCGAGACGCCGGACACCTGGCTTTCGCTCTGGGACGAGAGCTATGCGGGC
GCCGTGGCCCTGCCCTCGCCCAGCAACTCCTCGGGCGTGCCCTTCGTCCTGTTCCTCGCCCGCTCGGTGCTGGGCGACAC
CTCGGAATCCCTCGATCCGACCTTCGCCAAGCTGAAGGAGCTGGACACGGGGCTTCTCTTCGACAGCTCGGGCGCAGCCT
CGAACGCCTTCCAGAGCAGCGAGGTCATCATCGGCGCCCATTTCAACGTCGGCGCCTGGGATCTGACCGACGGCGGCCTG
CCCATCGGCTTCAGCGTGCCGAAGGAGGGCGTCTGGGCCACCGATGCGCGGATGCATGTGGTGAAGGGCACGAAGAACCC
CGAGGGCGCGGCGCAGTATCTCGACATGGCGGCCTCGCCCGAGGCGGCGGCCTGCCTGGCCGAACGGCTCTATCTCGGCC
CGCCCGTCACCGGCGTCACGCTCGCGCCCGATGTCGAGCGCAAGCTGCCCTGGGGCGAGGGCGGATCGGTCGAGAAGCTG
CATCTGTCGGACTGGACCGAGGTGAACGCGCGCCGCGCCGCCATCGTCGAACGCTGGAACCGCGAGATCGCGAACTGA

Upstream 100 bases:

>100_bases
TCAATCATGTGGCCGATGCATTCAATGGGATATGTCACATCGCTGCTAGGCTTCCTCCCAATCAAGAAGACTGCGCATCC
ACAGACAGGGAGTTCAAAAC

Downstream 100 bases:

>100_bases
GATCCGATGACCCTCCTCGCCATCTCCGACATCACCAAGAGCTTCGCGGGCCACAAGGCGCTCGATCATGTCACGCTCGA
CATCCGGCAGGGTGAGTTCA

Product: ABC polyamine/opine transporter, periplasmic binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MMDRVCRLSLAALLAGSGAAYADGSTLTVAYYGGNWGEAFDACVAQPFTEKTGIRVVAEIGNSTTTLAKLQQQSGDAVID
VAYMDGGISELAQEAGVLAPIDLAAIPNAASYLPQAVYKAGDEVFAVSAGYYSLGLIYNTSEVTETPDTWLSLWDESYAG
AVALPSPSNSSGVPFVLFLARSVLGDTSESLDPTFAKLKELDTGLLFDSSGAASNAFQSSEVIIGAHFNVGAWDLTDGGL
PIGFSVPKEGVWATDARMHVVKGTKNPEGAAQYLDMAASPEAAACLAERLYLGPPVTGVTLAPDVERKLPWGEGGSVEKL
HLSDWTEVNARRAAIVERWNREIAN

Sequences:

>Translated_345_residues
MMDRVCRLSLAALLAGSGAAYADGSTLTVAYYGGNWGEAFDACVAQPFTEKTGIRVVAEIGNSTTTLAKLQQQSGDAVID
VAYMDGGISELAQEAGVLAPIDLAAIPNAASYLPQAVYKAGDEVFAVSAGYYSLGLIYNTSEVTETPDTWLSLWDESYAG
AVALPSPSNSSGVPFVLFLARSVLGDTSESLDPTFAKLKELDTGLLFDSSGAASNAFQSSEVIIGAHFNVGAWDLTDGGL
PIGFSVPKEGVWATDARMHVVKGTKNPEGAAQYLDMAASPEAAACLAERLYLGPPVTGVTLAPDVERKLPWGEGGSVEKL
HLSDWTEVNARRAAIVERWNREIAN
>Mature_345_residues
MMDRVCRLSLAALLAGSGAAYADGSTLTVAYYGGNWGEAFDACVAQPFTEKTGIRVVAEIGNSTTTLAKLQQQSGDAVID
VAYMDGGISELAQEAGVLAPIDLAAIPNAASYLPQAVYKAGDEVFAVSAGYYSLGLIYNTSEVTETPDTWLSLWDESYAG
AVALPSPSNSSGVPFVLFLARSVLGDTSESLDPTFAKLKELDTGLLFDSSGAASNAFQSSEVIIGAHFNVGAWDLTDGGL
PIGFSVPKEGVWATDARMHVVKGTKNPEGAAQYLDMAASPEAAACLAERLYLGPPVTGVTLAPDVERKLPWGEGGSVEKL
HLSDWTEVNARRAAIVERWNREIAN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=314, Percent_Identity=24.8407643312102, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36266; Mature: 36266

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDRVCRLSLAALLAGSGAAYADGSTLTVAYYGGNWGEAFDACVAQPFTEKTGIRVVAEI
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEC
GNSTTTLAKLQQQSGDAVIDVAYMDGGISELAQEAGVLAPIDLAAIPNAASYLPQAVYKA
CCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHC
GDEVFAVSAGYYSLGLIYNTSEVTETPDTWLSLWDESYAGAVALPSPSNSSGVPFVLFLA
CCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHH
RSVLGDTSESLDPTFAKLKELDTGLLFDSSGAASNAFQSSEVIIGAHFNVGAWDLTDGGL
HHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEECCCCE
PIGFSVPKEGVWATDARMHVVKGTKNPEGAAQYLDMAASPEAAACLAERLYLGPPVTGVT
EECCCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEE
LAPDVERKLPWGEGGSVEKLHLSDWTEVNARRAAIVERWNREIAN
ECCCHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMDRVCRLSLAALLAGSGAAYADGSTLTVAYYGGNWGEAFDACVAQPFTEKTGIRVVAEI
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEC
GNSTTTLAKLQQQSGDAVIDVAYMDGGISELAQEAGVLAPIDLAAIPNAASYLPQAVYKA
CCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHC
GDEVFAVSAGYYSLGLIYNTSEVTETPDTWLSLWDESYAGAVALPSPSNSSGVPFVLFLA
CCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHH
RSVLGDTSESLDPTFAKLKELDTGLLFDSSGAASNAFQSSEVIIGAHFNVGAWDLTDGGL
HHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEECCCCE
PIGFSVPKEGVWATDARMHVVKGTKNPEGAAQYLDMAASPEAAACLAERLYLGPPVTGVT
EECCCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEE
LAPDVERKLPWGEGGSVEKLHLSDWTEVNARRAAIVERWNREIAN
ECCCHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]