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The map label for this gene is cfxB

Identifier: 77465276

GI number: 77465276

Start: 327559

End: 328623

Strand: Direct

Name: cfxB

Synonym: RSP_3270

Alternate gene names: 77465276

Gene position: 327559-328623 (Clockwise)

Preceding gene: 77465275

Following gene: 77465277

Centisome position: 34.74

GC content: 64.88

Gene sequence:

>1065_bases
ATGGCACTCATCACGCTTCGCCAGCTTCTCGATCACGCGGCCGAACAGGGCTACGGCGTTCCCGCCTTCAACATCAACAA
CATGGAGCAGGGTCTCGCGATCATGGAGGCGGCGCGGGCCTGCGATGCGCCCGTCATCATCCAGGCCAGCCGCGGCGCGC
GCAGCTACGCCAACGACATCATGCTGGCCAAGATGATCGAGGCGCTGGCCGCGATCTATCCCGAGATCCCGCTCTGCATG
CATCAGGACCACGGCAACAACGAGGCCACCTGCATGACCGCGATCCGGCACGGCTTCACCTCGGTGATGATGGACGGCTC
ACTGAAGGCCGATGCCAAGACGCCTGCCGATTATGACTACAATGTCGATATCACCGCCCGCGTGAGCCACATGGCGCATT
GGGTGGGCGCCTCGGTCGAGGGTGAGCTGGGCGTTCTGGGCAGCCTCGAGACCGGCGAGAGCGAGGCCGAGGACGGGCAT
GGCGCGGAAGGCAAGCTCGATCACAGCCAGCTGCTGACCGACCCCGATCAGGCGGTGGATTTCGTGAAGAAGACCCAGGT
CGATGCGCTGGCCATCGCCTGCGGCACCTCGCACGGCGCCTACAAGTTCAGCCGCAAGCCCGACGGCGAGATCCTCGCCA
TGTCGGTGATCGAGGCGATCCACAAGAAGCTGCCCGACACCCATCTCGTGATGCACGGATCCTCGTCGGTGCCGCAGGAG
CTGCAGGACATCATCAACGCGTTCGGCGGCGCCATGCCGCAGACCTTCGGCGTGCCGGTCGAGGAGATCGTGCGCGGCAT
CAAGATGGGCGTGCGCAAGGTCAATATCGACACCGACTGCCGCATGGCGATGACCGGGCAGTTCCGCCGCATCGCGCAGC
AGACGCCTTCCGAATTCGACCCGCGCAAGTTCCTCAAGCCCGCGATGGATGCGATGCGCGACCTCTGCAAGCAGCGGCTC
GAAGCCTTCGGCACCGCAGGCCAGGCCGGGAAGATCAGGATCATCCCGATGGACGATATGGCCAAACGCTATGCCAGCGG
CGCGCTTGCGCCCAAGACCGCCTGA

Upstream 100 bases:

>100_bases
GATGGGCCGGCTTCTCTGACCAAGCCGGGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTCGGCCAGAATGAAAGGGCC
GACAGGCAAGGACTTCACCC

Downstream 100 bases:

>100_bases
AAAGCCTCGTGTGAAAGGATACGATCATGGACCAGTCCAACCGCTACGCCCGGCTTGATCTGCAGGAAGCCGATCTGATC
GCCGGCGGCCGTCACGTTCT

Product: fructose-1,6-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; Fructose-bisphosphate aldolase II

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MALITLRQLLDHAAEQGYGVPAFNINNMEQGLAIMEAARACDAPVIIQASRGARSYANDIMLAKMIEALAAIYPEIPLCM
HQDHGNNEATCMTAIRHGFTSVMMDGSLKADAKTPADYDYNVDITARVSHMAHWVGASVEGELGVLGSLETGESEAEDGH
GAEGKLDHSQLLTDPDQAVDFVKKTQVDALAIACGTSHGAYKFSRKPDGEILAMSVIEAIHKKLPDTHLVMHGSSSVPQE
LQDIINAFGGAMPQTFGVPVEEIVRGIKMGVRKVNIDTDCRMAMTGQFRRIAQQTPSEFDPRKFLKPAMDAMRDLCKQRL
EAFGTAGQAGKIRIIPMDDMAKRYASGALAPKTA

Sequences:

>Translated_354_residues
MALITLRQLLDHAAEQGYGVPAFNINNMEQGLAIMEAARACDAPVIIQASRGARSYANDIMLAKMIEALAAIYPEIPLCM
HQDHGNNEATCMTAIRHGFTSVMMDGSLKADAKTPADYDYNVDITARVSHMAHWVGASVEGELGVLGSLETGESEAEDGH
GAEGKLDHSQLLTDPDQAVDFVKKTQVDALAIACGTSHGAYKFSRKPDGEILAMSVIEAIHKKLPDTHLVMHGSSSVPQE
LQDIINAFGGAMPQTFGVPVEEIVRGIKMGVRKVNIDTDCRMAMTGQFRRIAQQTPSEFDPRKFLKPAMDAMRDLCKQRL
EAFGTAGQAGKIRIIPMDDMAKRYASGALAPKTA
>Mature_353_residues
ALITLRQLLDHAAEQGYGVPAFNINNMEQGLAIMEAARACDAPVIIQASRGARSYANDIMLAKMIEALAAIYPEIPLCMH
QDHGNNEATCMTAIRHGFTSVMMDGSLKADAKTPADYDYNVDITARVSHMAHWVGASVEGELGVLGSLETGESEAEDGHG
AEGKLDHSQLLTDPDQAVDFVKKTQVDALAIACGTSHGAYKFSRKPDGEILAMSVIEAIHKKLPDTHLVMHGSSSVPQEL
QDIINAFGGAMPQTFGVPVEEIVRGIKMGVRKVNIDTDCRMAMTGQFRRIAQQTPSEFDPRKFLKPAMDAMRDLCKQRLE
AFGTAGQAGKIRIIPMDDMAKRYASGALAPKTA

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family

Homologues:

Organism=Escherichia coli, GI1789526, Length=329, Percent_Identity=31.6109422492401, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082041, Length=333, Percent_Identity=30.03003003003, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1788072, Length=328, Percent_Identity=27.7439024390244, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322790, Length=370, Percent_Identity=27.027027027027, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALF2_RHOSH (P29271)

Other databases:

- EMBL:   M68914
- PIR:   D41080
- ProteinModelPortal:   P29271
- SMR:   P29271
- BRENDA:   4.1.2.13
- GO:   GO:0006096
- InterPro:   IPR013785
- InterPro:   IPR006412
- InterPro:   IPR000771
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF001359
- TIGRFAMs:   TIGR00167
- TIGRFAMs:   TIGR01521

Pfam domain/function: PF01116 F_bP_aldolase

EC number: =4.1.2.13

Molecular weight: Translated: 38269; Mature: 38138

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00602 ALDOLASE_CLASS_II_1; PS00806 ALDOLASE_CLASS_II_2

Important sites: ACT_SITE 83-83 BINDING 50-50 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALITLRQLLDHAAEQGYGVPAFNINNMEQGLAIMEAARACDAPVIIQASRGARSYANDI
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
MLAKMIEALAAIYPEIPLCMHQDHGNNEATCMTAIRHGFTSVMMDGSLKADAKTPADYDY
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NVDITARVSHMAHWVGASVEGELGVLGSLETGESEAEDGHGAEGKLDHSQLLTDPDQAVD
CCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHH
FVKKTQVDALAIACGTSHGAYKFSRKPDGEILAMSVIEAIHKKLPDTHLVMHGSSSVPQE
HHHHHHHHHEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
LQDIINAFGGAMPQTFGVPVEEIVRGIKMGVRKVNIDTDCRMAMTGQFRRIAQQTPSEFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
PRKFLKPAMDAMRDLCKQRLEAFGTAGQAGKIRIIPMDDMAKRYASGALAPKTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALITLRQLLDHAAEQGYGVPAFNINNMEQGLAIMEAARACDAPVIIQASRGARSYANDI
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
MLAKMIEALAAIYPEIPLCMHQDHGNNEATCMTAIRHGFTSVMMDGSLKADAKTPADYDY
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NVDITARVSHMAHWVGASVEGELGVLGSLETGESEAEDGHGAEGKLDHSQLLTDPDQAVD
CCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHH
FVKKTQVDALAIACGTSHGAYKFSRKPDGEILAMSVIEAIHKKLPDTHLVMHGSSSVPQE
HHHHHHHHHEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
LQDIINAFGGAMPQTFGVPVEEIVRGIKMGVRKVNIDTDCRMAMTGQFRRIAQQTPSEFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
PRKFLKPAMDAMRDLCKQRLEAFGTAGQAGKIRIIPMDDMAKRYASGALAPKTA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939098