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The map label for this gene is gapB

Identifier: 77465275

GI number: 77465275

Start: 326476

End: 327477

Strand: Direct

Name: gapB

Synonym: RSP_3269

Alternate gene names: 77465275

Gene position: 326476-327477 (Clockwise)

Preceding gene: 77465274

Following gene: 77465276

Centisome position: 34.62

GC content: 66.37

Gene sequence:

>1002_bases
ATGACAATCAGGGTTGCCATCAACGGCTTCGGCCGTATCGGCCGCAACGTGCTGCGGGCCATCGTGGAGTCGGGGCGCAC
CGATATCGAGGTGGTCGCGATCAACGATCTGGGTCAGGTCGAGACCAACGCGCATCTTCTGCGCTTCGACAGCGTGCACG
GCCGCTTCCCGGCCAAGGTCACCAGCGGAGACGACTGGATCGATGTGGGCCGCGGCCCGATCAAGGTGACGGCGATCCGC
AATCCGGCGGAGCTGCCCTGGGCGGGTGTCGACATGGCGATGGAATGCACGGGCATCTTCACCACCAAGGAGAAGGCCGC
GGCCCATCTGCAGAACGGGGCGAAGCGGGTGCTCGTCTCGGCGCCCTGCGACGGGGCCGACCGGACCATCGTCTATGGGG
TGAACCATGCGACGCTCACCGCGGACGACCTCGTGGTCTCGAATGCCTCCTGCACCACCAACTGCCTCTCGCCGGTGGCC
AAGGTGCTTCACGATGCGATCGGCATCGCCAAGGGCTTCATGACCACGATCCACAGCTATACGGGCGACCAGCCCACGCT
CGACACGATGCACAAGGATCTCTACCGCGCCCGCGCCGCGGCGCTGAGCATGATCCCCACCTCGACGGGAGCGGCGAAGG
CGGTGGGCCTCGTGCTGCCCGAGCTCAAGGGCCGGCTCGACGGCGTGTCGATCCGGGTGCCCACGCCCAATGTCTCGGTG
GTGGATCTGGTGTTCGAGGCCGCCCGCGACACGACGGTGGAGGAGGTGAATGCGGCCATCGAGGCCGCCGCCTGCGGACC
GTTGAAGGGCGTGCTGGGCTTCACGACCGAGCCCAACGTCTCGTCCGACTTCAACCACGACCCGCATTCGTCGGTGTTCC
ACATGGACCAGACCAAGGTGATGGAGGGCCGCATGGTCCGCATCCTCAGCTGGTACGACAACGAATGGGGCTTCTCGAAC
CGGATGGCCGACACCGCCGTGGCGATGGGCCGGCTTCTCTGA

Upstream 100 bases:

>100_bases
TTCGGCGCTTCGGCGCCCGCGCCCGCACTCTATCAACATTTCAACATCACCGCTGAAGCCATTGTCAAATCGGCAAAAGA
ACGGATCTGAGGGAAGAAGG

Downstream 100 bases:

>100_bases
CCAAGCCGGGGGGCGCTGCCCCCCGGACCCCCCGGGATATTTCGGCCAGAATGAAAGGGCCGACAGGCAAGGACTTCACC
CATGGCACTCATCACGCTTC

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTIRVAINGFGRIGRNVLRAIVESGRTDIEVVAINDLGQVETNAHLLRFDSVHGRFPAKVTSGDDWIDVGRGPIKVTAIR
NPAELPWAGVDMAMECTGIFTTKEKAAAHLQNGAKRVLVSAPCDGADRTIVYGVNHATLTADDLVVSNASCTTNCLSPVA
KVLHDAIGIAKGFMTTIHSYTGDQPTLDTMHKDLYRARAAALSMIPTSTGAAKAVGLVLPELKGRLDGVSIRVPTPNVSV
VDLVFEAARDTTVEEVNAAIEAAACGPLKGVLGFTTEPNVSSDFNHDPHSSVFHMDQTKVMEGRMVRILSWYDNEWGFSN
RMADTAVAMGRLL

Sequences:

>Translated_333_residues
MTIRVAINGFGRIGRNVLRAIVESGRTDIEVVAINDLGQVETNAHLLRFDSVHGRFPAKVTSGDDWIDVGRGPIKVTAIR
NPAELPWAGVDMAMECTGIFTTKEKAAAHLQNGAKRVLVSAPCDGADRTIVYGVNHATLTADDLVVSNASCTTNCLSPVA
KVLHDAIGIAKGFMTTIHSYTGDQPTLDTMHKDLYRARAAALSMIPTSTGAAKAVGLVLPELKGRLDGVSIRVPTPNVSV
VDLVFEAARDTTVEEVNAAIEAAACGPLKGVLGFTTEPNVSSDFNHDPHSSVFHMDQTKVMEGRMVRILSWYDNEWGFSN
RMADTAVAMGRLL
>Mature_332_residues
TIRVAINGFGRIGRNVLRAIVESGRTDIEVVAINDLGQVETNAHLLRFDSVHGRFPAKVTSGDDWIDVGRGPIKVTAIRN
PAELPWAGVDMAMECTGIFTTKEKAAAHLQNGAKRVLVSAPCDGADRTIVYGVNHATLTADDLVVSNASCTTNCLSPVAK
VLHDAIGIAKGFMTTIHSYTGDQPTLDTMHKDLYRARAAALSMIPTSTGAAKAVGLVLPELKGRLDGVSIRVPTPNVSVV
DLVFEAARDTTVEEVNAAIEAAACGPLKGVLGFTTEPNVSSDFNHDPHSSVFHMDQTKVMEGRMVRILSWYDNEWGFSNR
MADTAVAMGRLL

Specific function: Could Play A Role In Pyridoxal 5'-Phosphate Synthesis. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7669492, Length=331, Percent_Identity=46.5256797583082, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI7657116, Length=327, Percent_Identity=43.7308868501529, Blast_Score=268, Evalue=5e-72,
Organism=Escherichia coli, GI1789295, Length=334, Percent_Identity=47.6047904191617, Blast_Score=313, Evalue=9e-87,
Organism=Escherichia coli, GI1788079, Length=327, Percent_Identity=49.5412844036697, Blast_Score=304, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI17534677, Length=330, Percent_Identity=48.4848484848485, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17534679, Length=330, Percent_Identity=48.1818181818182, Blast_Score=300, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI32566163, Length=330, Percent_Identity=47.5757575757576, Blast_Score=292, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17568413, Length=330, Percent_Identity=47.5757575757576, Blast_Score=292, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6322409, Length=326, Percent_Identity=48.159509202454, Blast_Score=303, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6321631, Length=326, Percent_Identity=48.159509202454, Blast_Score=297, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6322468, Length=326, Percent_Identity=47.8527607361963, Blast_Score=296, Evalue=4e-81,
Organism=Drosophila melanogaster, GI85725000, Length=325, Percent_Identity=48.3076923076923, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI22023983, Length=325, Percent_Identity=48.3076923076923, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI17933600, Length=325, Percent_Identity=48.3076923076923, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI18110149, Length=325, Percent_Identity=48.3076923076923, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI19922412, Length=325, Percent_Identity=41.8461538461538, Blast_Score=248, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): G3P2_RHOSH (P29272)

Other databases:

- EMBL:   M68914
- PIR:   C41080
- ProteinModelPortal:   P29272
- SMR:   P29272
- BRENDA:   1.2.1.12
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 35679; Mature: 35548

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 151-151 BINDING 36-36 BINDING 80-80 BINDING 181-181 BINDING 196-196 BINDING 232-232 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRVAINGFGRIGRNVLRAIVESGRTDIEVVAINDLGQVETNAHLLRFDSVHGRFPAKV
CEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEE
TSGDDWIDVGRGPIKVTAIRNPAELPWAGVDMAMECTGIFTTKEKAAAHLQNGAKRVLVS
CCCCCEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEEEECHHHHHHHHHCCCEEEEEE
APCDGADRTIVYGVNHATLTADDLVVSNASCTTNCLSPVAKVLHDAIGIAKGFMTTIHSY
CCCCCCCCEEEEECCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TGDQPTLDTMHKDLYRARAAALSMIPTSTGAAKAVGLVLPELKGRLDGVSIRVPTPNVSV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHH
VDLVFEAARDTTVEEVNAAIEAAACGPLKGVLGFTTEPNVSSDFNHDPHSSVFHMDQTKV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHH
MEGRMVRILSWYDNEWGFSNRMADTAVAMGRLL
HCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TIRVAINGFGRIGRNVLRAIVESGRTDIEVVAINDLGQVETNAHLLRFDSVHGRFPAKV
EEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEE
TSGDDWIDVGRGPIKVTAIRNPAELPWAGVDMAMECTGIFTTKEKAAAHLQNGAKRVLVS
CCCCCEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEEEECHHHHHHHHHCCCEEEEEE
APCDGADRTIVYGVNHATLTADDLVVSNASCTTNCLSPVAKVLHDAIGIAKGFMTTIHSY
CCCCCCCCEEEEECCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TGDQPTLDTMHKDLYRARAAALSMIPTSTGAAKAVGLVLPELKGRLDGVSIRVPTPNVSV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHH
VDLVFEAARDTTVEEVNAAIEAAACGPLKGVLGFTTEPNVSSDFNHDPHSSVFHMDQTKV
HHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHH
MEGRMVRILSWYDNEWGFSNRMADTAVAMGRLL
HCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939098