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The map label for this gene is dppA [H]

Identifier: 77465243

GI number: 77465243

Start: 289521

End: 291119

Strand: Direct

Name: dppA [H]

Synonym: RSP_3231

Alternate gene names: 77465243

Gene position: 289521-291119 (Clockwise)

Preceding gene: 77465240

Following gene: 77465244

Centisome position: 30.7

GC content: 63.79

Gene sequence:

>1599_bases
ATGAAATTCGCACATCTGCTGGCCGCGTCTTCGCTGGCCCTGATGGCTGCTGCCGGAGCCCACGCCAAGACGCTGGTCTA
TTGCTCGGAAGGGTCGCCCGAGGGGTTCGACCCCGCGCCCTACACCGCCGGCACCACCTTCGACGCGGCCTCGCAGGCGG
TCTACAACCAGCTCGTCGAGTTCAAGCCGGGCACGACCGAGATCGCCCCCGCGCTGGCCGAGAGCTATGAGATCTCGGAC
GACGGGCTGGAATACACCTTCCACCTGCGGCCGGGCGTCAAGTTCCACACGACCGACTTCTTCACGCCCACGCGCGAGAT
GAACGCCGACGACGTGATCTTCTCGTTCCTGCGTCAGGGCGACGAATCCAGCCCGTGGCACCAGTATGTGGCCGGGATCA
CCTACGAATATTACAGCGGCATGGAAATGCCGACCGTGATCAAGGAGATCCAGAAGGTCGACGACCTGACGGTGAAGTTC
GTGCTGACCCGTCCCGAGGCGCCCTTCCTCGCCAATCTCGCGATGGACTTCGCCTCGATCCTGTCGAAGGAATATGCCGA
CAAGCTGGAGGCCGAGAACCGCAAGGAAGACCTGAACAACGCGCCGGTCGGCACCGGCCCGTTCAAGTTCGTGGCCTACC
AGAAGGACGCGGTCATCCGCTATCAGGCCAATGACGACTACTGGGCCGGGCGCGAGAAGATCGACGATCTGATCTTCGCC
ATCACCCCCGATCCGGCGGTGCGGATGCAGAAGCTGCAGGCCGGCGAATGCCACATCATGCCCTATCCGGCGCCCGCCGA
CATCGAGGCGCTGAAGGCCGACGAGAACCTGCAGGTGATGGAGCAGCCGGGCCTGAACGTGGCCTATCTCGCCTACAACA
CCACCGTGGCGCCCTTCGACAATCCGAACGTCCGCAAGGCGCTCAACATGGCGATGAACAAGGAGGCCATCCTCGAGGCG
GTCTTCCAGGGCACGGGGCAGGTCGCCAAGAACCCGATCCCGCCGACCATGTGGAGCTACAACGACGCGGTCGAGGACAC
GGCCTTCGATCCCGAAGCGGCCAAGAAGCTCCTCGAGGAAGCCGGCGTGTCGGATCTCTCGATGGAGATCTGGGCGATGC
CGGTGCAGCGTCCCTACATGCCGAACGCCCGGCGCACGGCCGAGCTGATGCAGGAAGACTTCGCCAAGATCGGCGTCAAG
GTCGAGATCGTCTCCTACGAGTGGGGCGAGTATCTGAAGAAATCGACCGACCCGTCGCGCAAGGGCGCGGTCATCCTCGG
CTGGACGGGCGACAACGGCGACCCGGACAACTTCATGGGCGTGCTGCTGGGCTGCTCGGCCACCGGCGACGGCGGCGCGA
ACCGCGCGCAATGGTGCAACAAGGAGTTCGACGACCTGATCCAGAAGGCGAAGGTCACGGCGGATCAGGCGGAGCGCACC
AAGCTCTACGAAGAGGCGCAGGTCGTCTTCAAGCGCGAGAACCCCTGGGCCACCATCGCCCATTCGACGGTCTTCATGCC
GATGTCGAAGAAGGTCTCGGGCTATGTGATGAACCCGCTGGGCAAGCACAGCTTCTCGGGCGTCGATATCGAAGAGTGA

Upstream 100 bases:

>100_bases
AGAATTCGCCTTGCAAAGGTTTTCAACAGCGCATTTGATCCTGTCGAGGCGCCGCTCGTTCGCAAGAAGGCGGCACTTCC
AACATGATGGAGGTTCACCC

Downstream 100 bases:

>100_bases
AACGGCTAAAGTAACGGGCAGGCGCGCCGGGAGCACCCCGGCGCGCCTCTCATCATTCAGGGCTCAGATGATCAGATTCC
TCCTCGGCAAGCTTGCCTAT

Product: ABC peptide transporter, periplasmic binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKFAHLLAASSLALMAAAGAHAKTLVYCSEGSPEGFDPAPYTAGTTFDAASQAVYNQLVEFKPGTTEIAPALAESYEISD
DGLEYTFHLRPGVKFHTTDFFTPTREMNADDVIFSFLRQGDESSPWHQYVAGITYEYYSGMEMPTVIKEIQKVDDLTVKF
VLTRPEAPFLANLAMDFASILSKEYADKLEAENRKEDLNNAPVGTGPFKFVAYQKDAVIRYQANDDYWAGREKIDDLIFA
ITPDPAVRMQKLQAGECHIMPYPAPADIEALKADENLQVMEQPGLNVAYLAYNTTVAPFDNPNVRKALNMAMNKEAILEA
VFQGTGQVAKNPIPPTMWSYNDAVEDTAFDPEAAKKLLEEAGVSDLSMEIWAMPVQRPYMPNARRTAELMQEDFAKIGVK
VEIVSYEWGEYLKKSTDPSRKGAVILGWTGDNGDPDNFMGVLLGCSATGDGGANRAQWCNKEFDDLIQKAKVTADQAERT
KLYEEAQVVFKRENPWATIAHSTVFMPMSKKVSGYVMNPLGKHSFSGVDIEE

Sequences:

>Translated_532_residues
MKFAHLLAASSLALMAAAGAHAKTLVYCSEGSPEGFDPAPYTAGTTFDAASQAVYNQLVEFKPGTTEIAPALAESYEISD
DGLEYTFHLRPGVKFHTTDFFTPTREMNADDVIFSFLRQGDESSPWHQYVAGITYEYYSGMEMPTVIKEIQKVDDLTVKF
VLTRPEAPFLANLAMDFASILSKEYADKLEAENRKEDLNNAPVGTGPFKFVAYQKDAVIRYQANDDYWAGREKIDDLIFA
ITPDPAVRMQKLQAGECHIMPYPAPADIEALKADENLQVMEQPGLNVAYLAYNTTVAPFDNPNVRKALNMAMNKEAILEA
VFQGTGQVAKNPIPPTMWSYNDAVEDTAFDPEAAKKLLEEAGVSDLSMEIWAMPVQRPYMPNARRTAELMQEDFAKIGVK
VEIVSYEWGEYLKKSTDPSRKGAVILGWTGDNGDPDNFMGVLLGCSATGDGGANRAQWCNKEFDDLIQKAKVTADQAERT
KLYEEAQVVFKRENPWATIAHSTVFMPMSKKVSGYVMNPLGKHSFSGVDIEE
>Mature_532_residues
MKFAHLLAASSLALMAAAGAHAKTLVYCSEGSPEGFDPAPYTAGTTFDAASQAVYNQLVEFKPGTTEIAPALAESYEISD
DGLEYTFHLRPGVKFHTTDFFTPTREMNADDVIFSFLRQGDESSPWHQYVAGITYEYYSGMEMPTVIKEIQKVDDLTVKF
VLTRPEAPFLANLAMDFASILSKEYADKLEAENRKEDLNNAPVGTGPFKFVAYQKDAVIRYQANDDYWAGREKIDDLIFA
ITPDPAVRMQKLQAGECHIMPYPAPADIEALKADENLQVMEQPGLNVAYLAYNTTVAPFDNPNVRKALNMAMNKEAILEA
VFQGTGQVAKNPIPPTMWSYNDAVEDTAFDPEAAKKLLEEAGVSDLSMEIWAMPVQRPYMPNARRTAELMQEDFAKIGVK
VEIVSYEWGEYLKKSTDPSRKGAVILGWTGDNGDPDNFMGVLLGCSATGDGGANRAQWCNKEFDDLIQKAKVTADQAERT
KLYEEAQVVFKRENPWATIAHSTVFMPMSKKVSGYVMNPLGKHSFSGVDIEE

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=526, Percent_Identity=57.9847908745247, Blast_Score=643, Evalue=0.0,
Organism=Escherichia coli, GI1787551, Length=504, Percent_Identity=36.7063492063492, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1787052, Length=527, Percent_Identity=27.3244781783681, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1787762, Length=551, Percent_Identity=27.5862068965517, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789887, Length=548, Percent_Identity=24.6350364963504, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789397, Length=511, Percent_Identity=23.2876712328767, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787495, Length=449, Percent_Identity=24.4988864142539, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58958; Mature: 58958

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAHLLAASSLALMAAAGAHAKTLVYCSEGSPEGFDPAPYTAGTTFDAASQAVYNQLVE
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FKPGTTEIAPALAESYEISDDGLEYTFHLRPGVKFHTTDFFTPTREMNADDVIFSFLRQG
CCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
DESSPWHQYVAGITYEYYSGMEMPTVIKEIQKVDDLTVKFVLTRPEAPFLANLAMDFASI
CCCCCHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHH
LSKEYADKLEAENRKEDLNNAPVGTGPFKFVAYQKDAVIRYQANDDYWAGREKIDDLIFA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHHCCEEEE
ITPDPAVRMQKLQAGECHIMPYPAPADIEALKADENLQVMEQPGLNVAYLAYNTTVAPFD
ECCCHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCEEEECCCCCEEEEEEECEECCCC
NPNVRKALNMAMNKEAILEAVFQGTGQVAKNPIPPTMWSYNDAVEDTAFDPEAAKKLLEE
CCHHHHHHHHHHCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGVSDLSMEIWAMPVQRPYMPNARRTAELMQEDFAKIGVKVEIVSYEWGEYLKKSTDPSR
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHCCCCCCC
KGAVILGWTGDNGDPDNFMGVLLGCSATGDGGANRAQWCNKEFDDLIQKAKVTADQAERT
CCEEEEEECCCCCCCHHHEEEEEECCCCCCCCCCHHHHHCHHHHHHHHHHHCCHHHHHHH
KLYEEAQVVFKRENPWATIAHSTVFMPMSKKVSGYVMNPLGKHSFSGVDIEE
HHHHHHHHEEECCCCCEEEECCEEEEECCCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKFAHLLAASSLALMAAAGAHAKTLVYCSEGSPEGFDPAPYTAGTTFDAASQAVYNQLVE
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FKPGTTEIAPALAESYEISDDGLEYTFHLRPGVKFHTTDFFTPTREMNADDVIFSFLRQG
CCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
DESSPWHQYVAGITYEYYSGMEMPTVIKEIQKVDDLTVKFVLTRPEAPFLANLAMDFASI
CCCCCHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHH
LSKEYADKLEAENRKEDLNNAPVGTGPFKFVAYQKDAVIRYQANDDYWAGREKIDDLIFA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHHCCEEEE
ITPDPAVRMQKLQAGECHIMPYPAPADIEALKADENLQVMEQPGLNVAYLAYNTTVAPFD
ECCCHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCEEEECCCCCEEEEEEECEECCCC
NPNVRKALNMAMNKEAILEAVFQGTGQVAKNPIPPTMWSYNDAVEDTAFDPEAAKKLLEE
CCHHHHHHHHHHCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGVSDLSMEIWAMPVQRPYMPNARRTAELMQEDFAKIGVKVEIVSYEWGEYLKKSTDPSR
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHCCCCCCC
KGAVILGWTGDNGDPDNFMGVLLGCSATGDGGANRAQWCNKEFDDLIQKAKVTADQAERT
CCEEEEEECCCCCCCHHHEEEEEECCCCCCCCCCHHHHHCHHHHHHHHHHHCCHHHHHHH
KLYEEAQVVFKRENPWATIAHSTVFMPMSKKVSGYVMNPLGKHSFSGVDIEE
HHHHHHHHEEECCCCCEEEECCEEEEECCCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]