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The map label for this gene is cobB [H]

Identifier: 77465238

GI number: 77465238

Start: 285189

End: 285881

Strand: Direct

Name: cobB [H]

Synonym: RSP_3226

Alternate gene names: 77465238

Gene position: 285189-285881 (Clockwise)

Preceding gene: 77465237

Following gene: 77465239

Centisome position: 30.24

GC content: 71.14

Gene sequence:

>693_bases
ATGGATTCGATCTTCATCCTGACCGGCGCCGGGATCTCGGCCGAAAGCGGGCTCGGCACTTTCCGGGACAAGGACGGCCT
CTGGACCCGCTACGACCTGAGCCGCGTGGCCACGCCCGAGGGCTTTCGCGCCGATCCCGCTCTGGTCCATGACTTCTACA
ACATGCGCCGGAAGAACTGCCTCGACGCGGCCCCCAACGCAGCCCATGCGGCCCTTGCGCGGCTCGGGCGCGACCATCCG
GGCGAGGTGACGCTGGTCACGCAGAATGTGGATGACCTGCACGAGCGGGCGGGCTCGACCGGCGTGATCCACATGCACGG
CGAGCTGCGCCGCGCGCTCTGCGCGGCCTGCGGCCACCGCTGGCCTGCGCCCGAGCGCATGAGGTCCGAGGACGCCTGCC
CGAGCTGCGGCCGGCCCGCCACCCGCCCCGACATCGTCTGGTTCGGCGAGATGCCCTATCGGATGGACGAGATCCTCGAG
CGGCTGGCGCGGGCCACGCTCTTCGTGGTGATCGGCAGTTCCGGAGAGGTCTATCCCGCCGCGGGCTTCGTCGACGAGGC
GGCGGCGCAGGGCATTCCCACGCTCGAGATCAATCTCGAGCCGAGCGGACGCCGCGGCCTGTTCGACCGCGGCCTCTACG
GGCCTGCCACCGAGGTCGTGCCGCGCTGGGTGGCCGGGCTCCTGCGCGACTAG

Upstream 100 bases:

>100_bases
CAACATACAGAATTCGTTTCCGAAAGATGCAAGCGCCGGGGCGGCGCCGGTCGACCTGCCGCGGCTGGCGCCGGAGCGGG
CGGACTGGTAGCTTCGGCCC

Downstream 100 bases:

>100_bases
CCCCCGCCGCAGCCGGGGGACATTCCCGCCGACAGGGCGGATTCGCCGCTTGCGTCCGCGCGAGGGTGCGACGATAAGGC
GCCCCTCTTTTGCGATGGAG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MDSIFILTGAGISAESGLGTFRDKDGLWTRYDLSRVATPEGFRADPALVHDFYNMRRKNCLDAAPNAAHAALARLGRDHP
GEVTLVTQNVDDLHERAGSTGVIHMHGELRRALCAACGHRWPAPERMRSEDACPSCGRPATRPDIVWFGEMPYRMDEILE
RLARATLFVVIGSSGEVYPAAGFVDEAAAQGIPTLEINLEPSGRRGLFDRGLYGPATEVVPRWVAGLLRD

Sequences:

>Translated_230_residues
MDSIFILTGAGISAESGLGTFRDKDGLWTRYDLSRVATPEGFRADPALVHDFYNMRRKNCLDAAPNAAHAALARLGRDHP
GEVTLVTQNVDDLHERAGSTGVIHMHGELRRALCAACGHRWPAPERMRSEDACPSCGRPATRPDIVWFGEMPYRMDEILE
RLARATLFVVIGSSGEVYPAAGFVDEAAAQGIPTLEINLEPSGRRGLFDRGLYGPATEVVPRWVAGLLRD
>Mature_230_residues
MDSIFILTGAGISAESGLGTFRDKDGLWTRYDLSRVATPEGFRADPALVHDFYNMRRKNCLDAAPNAAHAALARLGRDHP
GEVTLVTQNVDDLHERAGSTGVIHMHGELRRALCAACGHRWPAPERMRSEDACPSCGRPATRPDIVWFGEMPYRMDEILE
RLARATLFVVIGSSGEVYPAAGFVDEAAAQGIPTLEINLEPSGRRGLFDRGLYGPATEVVPRWVAGLLRD

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=238, Percent_Identity=36.9747899159664, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI6912664, Length=251, Percent_Identity=35.0597609561753, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI13787215, Length=226, Percent_Identity=36.7256637168142, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI6912662, Length=260, Percent_Identity=29.6153846153846, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI63054862, Length=206, Percent_Identity=31.0679611650485, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI6912660, Length=209, Percent_Identity=30.622009569378, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI7657575, Length=226, Percent_Identity=30.5309734513274, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI308199517, Length=229, Percent_Identity=52.8384279475983, Blast_Score=229, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17541892, Length=220, Percent_Identity=30.4545454545455, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28571445, Length=123, Percent_Identity=36.5853658536585, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 25154; Mature: 25154

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIFILTGAGISAESGLGTFRDKDGLWTRYDLSRVATPEGFRADPALVHDFYNMRRKNC
CCCEEEEECCCCCCCCCCCCCCCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LDAAPNAAHAALARLGRDHPGEVTLVTQNVDDLHERAGSTGVIHMHGELRRALCAACGHR
HCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCC
WPAPERMRSEDACPSCGRPATRPDIVWFGEMPYRMDEILERLARATLFVVIGSSGEVYPA
CCCHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEECCCCCEECC
AGFVDEAAAQGIPTLEINLEPSGRRGLFDRGLYGPATEVVPRWVAGLLRD
CCCHHHHHHCCCCEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSIFILTGAGISAESGLGTFRDKDGLWTRYDLSRVATPEGFRADPALVHDFYNMRRKNC
CCCEEEEECCCCCCCCCCCCCCCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LDAAPNAAHAALARLGRDHPGEVTLVTQNVDDLHERAGSTGVIHMHGELRRALCAACGHR
HCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCC
WPAPERMRSEDACPSCGRPATRPDIVWFGEMPYRMDEILERLARATLFVVIGSSGEVYPA
CCCHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEECCCCCEECC
AGFVDEAAAQGIPTLEINLEPSGRRGLFDRGLYGPATEVVPRWVAGLLRD
CCCHHHHHHCCCCEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]