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The map label for this gene is sdhA [C]

Identifier: 77465161

GI number: 77465161

Start: 196640

End: 198403

Strand: Reverse

Name: sdhA [C]

Synonym: RSP_3147

Alternate gene names: 77465161

Gene position: 198403-196640 (Counterclockwise)

Preceding gene: 77465162

Following gene: 77465160

Centisome position: 21.04

GC content: 69.95

Gene sequence:

>1764_bases
ATGCAGCACGACCGGCTGGAACGGCAGGAGACCGACATCCTGATCCTGGGCTCGGGCGGCGCGGGGCTCTTTGCCGCCCT
CCACGCCCAGCAGAGCGCGCCCACGGGCACCCGGATCACCATTGCGGTGAAGGGGCTTCTCGGGAAATGCGGCTGCACGC
GCATGGTGCAGGGCGGCTATAATGTGGCGCTCGGCGCGGGCGACACGGTCGAGCGGCACTTCATGGACACGATCCACGGC
GGCAAGTGGCTGCCCGATCAGGACATGGCCTGGCGGCTCTGCCAGGAGGCGGTGACGCGGGTGCGCGAACTGGAGAACGA
GGTCGGCTGTTTCTTCGACCGCAACCCGGACGGCACGCTGCACCACAAGGCCTTCGCGGGCCAGACCGCCGACCGGACTG
TCCACAAGGGCGATCTGACCGGCATCGAGATCATCAACCGGCTGATGGAGCAGGTGCTCGCGCGTCCGATCGAGCGGCTG
CAGGAGCATCGCGCCGTGGGCCTCATCCCGGCCGAGGGCGGCGGGGCGATCTCGGGCGTGCTCTTCATCGACATGCGCAC
GGGCCGCTTCCGCTTCGTGCGGGCGAAGAAGGTGCTGATGGCCACGGGCGGCGGGCCCACCATGTACAAGTATCACACGC
CATCGGGCGACAAGACGATGGACGGGCTGTCGATGGCGCTGCGTCTGGGCCTGCCGCTCCGCGACATGGAGATGGTGCAG
TTCCACCCGACCGGGCTACTCGCGGGGCCGGGCACGCGCATGACGGGCACCGTCCTCGAGGAAGGTCTGCGCGGCGCGGG
CGGGCATCTGCTGAACGGGCACGGCCACCGCTTCATGTATGACCATGATGCGGCGGGCGAGCGGGCCACGCGCGACGTGG
TCAGCCGCGGCATCTATGCCGAGATGCGCAAGAGCAACACCTCCGAGAACGGCGGCGTCTATATCTCGATGGCCCATCTC
GGCCCCGACGATGTCCGCCGCCGCTTCAAGGGCATGGTCAAGCGCTGCGCCGACTGCGGCTTCGATCTGGCCGGCGGGCT
GGTCGAGGTGGTGCCCACCGCGCATTACTTCATGGGCGGCGTCGTGGTGGATCCCGACACCCGCACCGAGATCGACGGGC
TGCATGTCGCGGGCGAGGATGCGGGCGGCGCGCATGGCGCGAACCGGCTGGGCGGCAACGGTGTCGCCAACTCCACCGTC
TTCGGCGGCATCGCGGGCGACGTGATGGGCGCCGAGGTGGCCTCGATCCGCCATCTGCGCCCCGCGGACGAGGCAGTGCT
CGCGGCCGAGATCGACCGTGCCATGGCGCCGCTCTCGAAGCGGCCGGGCCAGGTGCAAGTGCTGCGCGAGGCGCTGCAGG
AGGCCATGTGGGAGGATGTGGGGGTCCTGCGCACCGGCGCGGGCCTCGAGCGCGGCCTCACGCGGGTGGCGGCCATCGGC
GCGGAACTGGCCGAGACCGGCGTCGATGGCGCGAACCTCGCCTTCAACCTCACCTGGCACGACTGGCTGAACATGCGCTC
GCTCGTGGACATATCCGAGGCCATCGCCACGGCGGGCCTTGCGCGCGAGAATTCGCGCGGGGCGCATTACCGCGAGGATC
ACCCCGAGGCGGGCGATCTGGACGCCTCCTACTTCACGCAGGTGCGCCGGTCGGGCGACGAGCTGCCCGTCACGCGCGAG
CCCGTGCGCTTCACCATCGTGCGGCCCGGCGAGACGATCCTGCCGGCCGATGAACCCACCACACTGGTAAAGGCAGCGGA
ATGA

Upstream 100 bases:

>100_bases
GAGATGCTGCCCTGGAGCGGGCGGCAGAAGACGCTGGCCGCCGCCACCGTCGCCGGCGCCTTCCTCGTGGCCGCGACCTT
CCTTCTGAGGGCGATCTGAC

Downstream 100 bases:

>100_bases
TCTCACTCGATCTGATCCAGCGGACGGCCGAGCGGCTGATGGAAAAGGCCGCCATCGAAATTCCCGAAGATTATCTCGAC
GGGCTGAAGGCCACGGCGGC

Product: putative succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MQHDRLERQETDILILGSGGAGLFAALHAQQSAPTGTRITIAVKGLLGKCGCTRMVQGGYNVALGAGDTVERHFMDTIHG
GKWLPDQDMAWRLCQEAVTRVRELENEVGCFFDRNPDGTLHHKAFAGQTADRTVHKGDLTGIEIINRLMEQVLARPIERL
QEHRAVGLIPAEGGGAISGVLFIDMRTGRFRFVRAKKVLMATGGGPTMYKYHTPSGDKTMDGLSMALRLGLPLRDMEMVQ
FHPTGLLAGPGTRMTGTVLEEGLRGAGGHLLNGHGHRFMYDHDAAGERATRDVVSRGIYAEMRKSNTSENGGVYISMAHL
GPDDVRRRFKGMVKRCADCGFDLAGGLVEVVPTAHYFMGGVVVDPDTRTEIDGLHVAGEDAGGAHGANRLGGNGVANSTV
FGGIAGDVMGAEVASIRHLRPADEAVLAAEIDRAMAPLSKRPGQVQVLREALQEAMWEDVGVLRTGAGLERGLTRVAAIG
AELAETGVDGANLAFNLTWHDWLNMRSLVDISEAIATAGLARENSRGAHYREDHPEAGDLDASYFTQVRRSGDELPVTRE
PVRFTIVRPGETILPADEPTTLVKAAE

Sequences:

>Translated_587_residues
MQHDRLERQETDILILGSGGAGLFAALHAQQSAPTGTRITIAVKGLLGKCGCTRMVQGGYNVALGAGDTVERHFMDTIHG
GKWLPDQDMAWRLCQEAVTRVRELENEVGCFFDRNPDGTLHHKAFAGQTADRTVHKGDLTGIEIINRLMEQVLARPIERL
QEHRAVGLIPAEGGGAISGVLFIDMRTGRFRFVRAKKVLMATGGGPTMYKYHTPSGDKTMDGLSMALRLGLPLRDMEMVQ
FHPTGLLAGPGTRMTGTVLEEGLRGAGGHLLNGHGHRFMYDHDAAGERATRDVVSRGIYAEMRKSNTSENGGVYISMAHL
GPDDVRRRFKGMVKRCADCGFDLAGGLVEVVPTAHYFMGGVVVDPDTRTEIDGLHVAGEDAGGAHGANRLGGNGVANSTV
FGGIAGDVMGAEVASIRHLRPADEAVLAAEIDRAMAPLSKRPGQVQVLREALQEAMWEDVGVLRTGAGLERGLTRVAAIG
AELAETGVDGANLAFNLTWHDWLNMRSLVDISEAIATAGLARENSRGAHYREDHPEAGDLDASYFTQVRRSGDELPVTRE
PVRFTIVRPGETILPADEPTTLVKAAE
>Mature_587_residues
MQHDRLERQETDILILGSGGAGLFAALHAQQSAPTGTRITIAVKGLLGKCGCTRMVQGGYNVALGAGDTVERHFMDTIHG
GKWLPDQDMAWRLCQEAVTRVRELENEVGCFFDRNPDGTLHHKAFAGQTADRTVHKGDLTGIEIINRLMEQVLARPIERL
QEHRAVGLIPAEGGGAISGVLFIDMRTGRFRFVRAKKVLMATGGGPTMYKYHTPSGDKTMDGLSMALRLGLPLRDMEMVQ
FHPTGLLAGPGTRMTGTVLEEGLRGAGGHLLNGHGHRFMYDHDAAGERATRDVVSRGIYAEMRKSNTSENGGVYISMAHL
GPDDVRRRFKGMVKRCADCGFDLAGGLVEVVPTAHYFMGGVVVDPDTRTEIDGLHVAGEDAGGAHGANRLGGNGVANSTV
FGGIAGDVMGAEVASIRHLRPADEAVLAAEIDRAMAPLSKRPGQVQVLREALQEAMWEDVGVLRTGAGLERGLTRVAAIG
AELAETGVDGANLAFNLTWHDWLNMRSLVDISEAIATAGLARENSRGAHYREDHPEAGDLDASYFTQVRRSGDELPVTRE
PVRFTIVRPGETILPADEPTTLVKAAE

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=525, Percent_Identity=31.6190476190476, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1790597, Length=544, Percent_Identity=31.6176470588235, Blast_Score=242, Evalue=6e-65,
Organism=Escherichia coli, GI1786942, Length=565, Percent_Identity=33.8053097345133, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788928, Length=547, Percent_Identity=31.0786106032907, Blast_Score=221, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI17550100, Length=536, Percent_Identity=32.2761194029851, Blast_Score=204, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=520, Percent_Identity=31.5384615384615, Blast_Score=195, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6322701, Length=585, Percent_Identity=30.0854700854701, Blast_Score=219, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6322416, Length=537, Percent_Identity=31.6573556797021, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6320788, Length=487, Percent_Identity=27.3100616016427, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137288, Length=527, Percent_Identity=33.2068311195446, Blast_Score=219, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24655642, Length=527, Percent_Identity=33.2068311195446, Blast_Score=219, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24655647, Length=527, Percent_Identity=33.2068311195446, Blast_Score=219, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24663005, Length=509, Percent_Identity=31.041257367387, Blast_Score=196, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 63152; Mature: 63152

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHDRLERQETDILILGSGGAGLFAALHAQQSAPTGTRITIAVKGLLGKCGCTRMVQGGY
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEECHHCCCCHHHHHCCCC
NVALGAGDTVERHFMDTIHGGKWLPDQDMAWRLCQEAVTRVRELENEVGCFFDRNPDGTL
EEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCE
HHKAFAGQTADRTVHKGDLTGIEIINRLMEQVLARPIERLQEHRAVGLIPAEGGGAISGV
EEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEE
LFIDMRTGRFRFVRAKKVLMATGGGPTMYKYHTPSGDKTMDGLSMALRLGLPLRDMEMVQ
EEEEEECCCEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEE
FHPTGLLAGPGTRMTGTVLEEGLRGAGGHLLNGHGHRFMYDHDAAGERATRDVVSRGIYA
ECCCEEEECCCCCCHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHH
EMRKSNTSENGGVYISMAHLGPDDVRRRFKGMVKRCADCGFDLAGGLVEVVPTAHYFMGG
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCC
VVVDPDTRTEIDGLHVAGEDAGGAHGANRLGGNGVANSTVFGGIAGDVMGAEVASIRHLR
EEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCC
PADEAVLAAEIDRAMAPLSKRPGQVQVLREALQEAMWEDVGVLRTGAGLERGLTRVAAIG
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHH
AELAETGVDGANLAFNLTWHDWLNMRSLVDISEAIATAGLARENSRGAHYREDHPEAGDL
HHHHHCCCCCCCEEEEEEHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DASYFTQVRRSGDELPVTREPVRFTIVRPGETILPADEPTTLVKAAE
CHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEECCCCCCCCEEEECC
>Mature Secondary Structure
MQHDRLERQETDILILGSGGAGLFAALHAQQSAPTGTRITIAVKGLLGKCGCTRMVQGGY
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEECHHCCCCHHHHHCCCC
NVALGAGDTVERHFMDTIHGGKWLPDQDMAWRLCQEAVTRVRELENEVGCFFDRNPDGTL
EEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCE
HHKAFAGQTADRTVHKGDLTGIEIINRLMEQVLARPIERLQEHRAVGLIPAEGGGAISGV
EEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEE
LFIDMRTGRFRFVRAKKVLMATGGGPTMYKYHTPSGDKTMDGLSMALRLGLPLRDMEMVQ
EEEEEECCCEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEE
FHPTGLLAGPGTRMTGTVLEEGLRGAGGHLLNGHGHRFMYDHDAAGERATRDVVSRGIYA
ECCCEEEECCCCCCHHHHHHHHCCCCCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHH
EMRKSNTSENGGVYISMAHLGPDDVRRRFKGMVKRCADCGFDLAGGLVEVVPTAHYFMGG
HHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHCC
VVVDPDTRTEIDGLHVAGEDAGGAHGANRLGGNGVANSTVFGGIAGDVMGAEVASIRHLR
EEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCC
PADEAVLAAEIDRAMAPLSKRPGQVQVLREALQEAMWEDVGVLRTGAGLERGLTRVAAIG
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHH
AELAETGVDGANLAFNLTWHDWLNMRSLVDISEAIATAGLARENSRGAHYREDHPEAGDL
HHHHHCCCCCCCEEEEEEHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DASYFTQVRRSGDELPVTREPVRFTIVRPGETILPADEPTTLVKAAE
CHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]