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The map label for this gene is fadB [C]

Identifier: 77465015

GI number: 77465015

Start: 29437

End: 30222

Strand: Reverse

Name: fadB [C]

Synonym: RSP_3833

Alternate gene names: 77465015

Gene position: 30222-29437 (Counterclockwise)

Preceding gene: 77465017

Following gene: 77465010

Centisome position: 3.2

GC content: 71.88

Gene sequence:

>786_bases
ATGACCCAGATCCTGCTGCGCGAGGACCGCGGCGCGGTGGCCACGCTGACGCTGAACCGGCCCGAGGCGCTGAATGCGCT
GTCGGATGCGATGCTCGCGGCGCTCAAGGAGCAGTTCCGCGCGCTGGCCGGGGACCGGACGATCCGTGTCGTGGTGATCC
GCGGCGCGGGCAAGGCCTTCTGCGCGGGCCACGACCTGCGCGAGATGCAGGCCGCGCGCCAGTCCGACGACCGGGGCGCC
GCCTATTTCTCGGACCTGTTCTCCCGCTGCGCGGCGGTCATGCAGGCGATTCCCGCCCTGCCCCAGCCGGTGATCGCCGA
GGTCCACGGCATCGCCACCGCGGCGGGCTGCCAGCTCGTCGCCTCCTGCGACATGGCGGTGGCGGCCCACGGCACGCGCT
TCGGGGTGAACGGGGTGAATATCGGGCTCTTCTGCTCGACGCCGATGGTGGCCCTGACCCGCGCCGTGCCGCGCAAGGTG
GCCTTCGAGATGCTCACGACCGGCGAGTTCATCGAGGCCGACCGCGCCCGCGAGGTGGGCCTCGTGAACCGGACGGTCCC
GCCCGAGGATCTTGCGGCCGAGGTGCAGAAACTGGCGGCGGTCGTGGCGGGCAAGCTCACCGCTGCGGTGCGGACCGGCA
AGCGGGCCTTCTACGATCAGGCGGGCATGGGGCTCGGCGCGGCCTACACGCTGACCGGCGCGGTGATGGCGGACAACATG
ATGTGGCGGGATACGGAAGAGGGCGTGGCGGCCTTCCTCGAGAAACGGGCGCCCGACTGGGCCTGA

Upstream 100 bases:

>100_bases
GCGAGGAACGCGGCCAACTCCGCCGCCTCCAGCTTCAGCCCCATGCTTTCCTCCCTGCTTCCGGCCCGCTAGGCTGACCG
CAAAGGGGAGGAGTGACAAG

Downstream 100 bases:

>100_bases
CGCGCTTCGCTTCAGGCAATGCCGCTCATGGATCGGGGATCCGGGGTCGTCTTGCGCCCTCAGGAAGGGGGCAGCAGGAA
GACTGTTCTCATCTGGCGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTQILLREDRGAVATLTLNRPEALNALSDAMLAALKEQFRALAGDRTIRVVVIRGAGKAFCAGHDLREMQAARQSDDRGA
AYFSDLFSRCAAVMQAIPALPQPVIAEVHGIATAAGCQLVASCDMAVAAHGTRFGVNGVNIGLFCSTPMVALTRAVPRKV
AFEMLTTGEFIEADRAREVGLVNRTVPPEDLAAEVQKLAAVVAGKLTAAVRTGKRAFYDQAGMGLGAAYTLTGAVMADNM
MWRDTEEGVAAFLEKRAPDWA

Sequences:

>Translated_261_residues
MTQILLREDRGAVATLTLNRPEALNALSDAMLAALKEQFRALAGDRTIRVVVIRGAGKAFCAGHDLREMQAARQSDDRGA
AYFSDLFSRCAAVMQAIPALPQPVIAEVHGIATAAGCQLVASCDMAVAAHGTRFGVNGVNIGLFCSTPMVALTRAVPRKV
AFEMLTTGEFIEADRAREVGLVNRTVPPEDLAAEVQKLAAVVAGKLTAAVRTGKRAFYDQAGMGLGAAYTLTGAVMADNM
MWRDTEEGVAAFLEKRAPDWA
>Mature_260_residues
TQILLREDRGAVATLTLNRPEALNALSDAMLAALKEQFRALAGDRTIRVVVIRGAGKAFCAGHDLREMQAARQSDDRGAA
YFSDLFSRCAAVMQAIPALPQPVIAEVHGIATAAGCQLVASCDMAVAAHGTRFGVNGVNIGLFCSTPMVALTRAVPRKVA
FEMLTTGEFIEADRAREVGLVNRTVPPEDLAAEVQKLAAVVAGKLTAAVRTGKRAFYDQAGMGLGAAYTLTGAVMADNMM
WRDTEEGVAAFLEKRAPDWA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=45.7831325301205, Blast_Score=220, Evalue=8e-58,
Organism=Homo sapiens, GI194097323, Length=265, Percent_Identity=31.3207547169811, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI70995211, Length=190, Percent_Identity=33.1578947368421, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI37594469, Length=201, Percent_Identity=29.8507462686567, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI37594471, Length=201, Percent_Identity=29.8507462686567, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4502327, Length=262, Percent_Identity=28.2442748091603, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=28.7292817679558, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=35.5212355212355, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=271, Percent_Identity=32.4723247232472, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=31.1284046692607, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=29.2134831460674, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=27.3076923076923, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790281, Length=183, Percent_Identity=26.775956284153, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=250, Percent_Identity=39.2, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=32.1428571428571, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17554946, Length=264, Percent_Identity=30.6818181818182, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=29.004329004329, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=198, Percent_Identity=28.2828282828283, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=220, Percent_Identity=28.1818181818182, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=238, Percent_Identity=26.0504201680672, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=23.046875, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=201, Percent_Identity=27.8606965174129, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=260, Percent_Identity=24.6153846153846, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=260, Percent_Identity=24.6153846153846, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=25.3968253968254, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922422, Length=257, Percent_Identity=36.5758754863813, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=30.2040816326531, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=30.2040816326531, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=28.8461538461538, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920382, Length=214, Percent_Identity=30.8411214953271, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24650670, Length=278, Percent_Identity=28.4172661870504, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357171, Length=265, Percent_Identity=24.1509433962264, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583165, Length=188, Percent_Identity=26.063829787234, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19921018, Length=188, Percent_Identity=23.936170212766, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27710; Mature: 27579

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQILLREDRGAVATLTLNRPEALNALSDAMLAALKEQFRALAGDRTIRVVVIRGAGKAF
CCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCE
CAGHDLREMQAARQSDDRGAAYFSDLFSRCAAVMQAIPALPQPVIAEVHGIATAAGCQLV
ECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ASCDMAVAAHGTRFGVNGVNIGLFCSTPMVALTRAVPRKVAFEMLTTGEFIEADRAREVG
HHCCHHHHCCCCEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
LVNRTVPPEDLAAEVQKLAAVVAGKLTAAVRTGKRAFYDQAGMGLGAAYTLTGAVMADNM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
MWRDTEEGVAAFLEKRAPDWA
CCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQILLREDRGAVATLTLNRPEALNALSDAMLAALKEQFRALAGDRTIRVVVIRGAGKAF
CCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCE
CAGHDLREMQAARQSDDRGAAYFSDLFSRCAAVMQAIPALPQPVIAEVHGIATAAGCQLV
ECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ASCDMAVAAHGTRFGVNGVNIGLFCSTPMVALTRAVPRKVAFEMLTTGEFIEADRAREVG
HHCCHHHHCCCCEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
LVNRTVPPEDLAAEVQKLAAVVAGKLTAAVRTGKRAFYDQAGMGLGAAYTLTGAVMADNM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
MWRDTEEGVAAFLEKRAPDWA
CCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA