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The map label for this gene is thiE [H]

Identifier: 77465010

GI number: 77465010

Start: 23965

End: 24585

Strand: Reverse

Name: thiE [H]

Synonym: RSP_3828

Alternate gene names: 77465010

Gene position: 24585-23965 (Counterclockwise)

Preceding gene: 77465015

Following gene: 77465006

Centisome position: 2.61

GC content: 71.66

Gene sequence:

>621_bases
ATGGCCGAAGTCGAACGTCCGCAGCTCTATCTGGTCACGCCGCCCGAGATCGATCTCGAGATCTTCCCCGACCGGCTGGC
GGCGGTGCTCGATTCGACCGAGGTGGCCTGCCTCAGGCTGGCGCTCGCCGGCAAGGACGAGGACCGGATCGCCCGGACGG
GCGATGCGCTGCGCGAGGTGGCCCATGCCCGCGACGTGGCGCTGGTGATCGAGAACCATGTGCTGATGGTCGAGCGGCTG
GGCCTCGACGGGGTGCATCTGACCGATGGCGCGCGGCTCGTGCGCAAGCTGCGCAAGGATCTGGGGCCGGATGCGATCCT
CGGCGCCTTCTGCGGCCGGACCAGGCACGAGGGGATCAACGCGGCCGAGGCGGGCGCGGATTATGTGGCCTTCGGCCCGG
TGGGCACGACCGCGCTCGGCGACGGCAGCCTGGCCGAGGCCGAGCTCTTCGGCTGGTGGTCCGAGATGATCGAGGTGCCG
GTGGTGGCCGAGGGCGCGCTCACCCCCGAGAAGGTGGCCGAGCTCGCCCCCCTCACCGACTTCTTCGCCGTGGGCGAGGA
GATCTGGCGCGAGGAGGATGCGGCGGCGGCGCTGCGCCGGTTGCTGGCCCCGCTGGGCTGA

Upstream 100 bases:

>100_bases
GATCCGGGCGCTTCCTAGGGGAGAAGGGCCCTGCGGCGCAATGCCATTTCCAAGCGCGCGCCCCTCGGCTATAGGCTTCG
CGATCCGAAGGAGACCCATC

Downstream 100 bases:

>100_bases
GGCGCGCCGGCCCCGGCCGGTCAGCCGACAGGCTCGGGCGGGAAGGCCTCGCGCACCAGCGCCTCGCAGCGGGCGGCGAG
GCCCTTCCGCCCCGCGAACC

Product: putative thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MAEVERPQLYLVTPPEIDLEIFPDRLAAVLDSTEVACLRLALAGKDEDRIARTGDALREVAHARDVALVIENHVLMVERL
GLDGVHLTDGARLVRKLRKDLGPDAILGAFCGRTRHEGINAAEAGADYVAFGPVGTTALGDGSLAEAELFGWWSEMIEVP
VVAEGALTPEKVAELAPLTDFFAVGEEIWREEDAAAALRRLLAPLG

Sequences:

>Translated_206_residues
MAEVERPQLYLVTPPEIDLEIFPDRLAAVLDSTEVACLRLALAGKDEDRIARTGDALREVAHARDVALVIENHVLMVERL
GLDGVHLTDGARLVRKLRKDLGPDAILGAFCGRTRHEGINAAEAGADYVAFGPVGTTALGDGSLAEAELFGWWSEMIEVP
VVAEGALTPEKVAELAPLTDFFAVGEEIWREEDAAAALRRLLAPLG
>Mature_205_residues
AEVERPQLYLVTPPEIDLEIFPDRLAAVLDSTEVACLRLALAGKDEDRIARTGDALREVAHARDVALVIENHVLMVERLG
LDGVHLTDGARLVRKLRKDLGPDAILGAFCGRTRHEGINAAEAGADYVAFGPVGTTALGDGSLAEAELFGWWSEMIEVPV
VAEGALTPEKVAELAPLTDFFAVGEEIWREEDAAAALRRLLAPLG

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22172; Mature: 22040

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVERPQLYLVTPPEIDLEIFPDRLAAVLDSTEVACLRLALAGKDEDRIARTGDALREV
CCCCCCCEEEEECCCCCCEEECHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
AHARDVALVIENHVLMVERLGLDGVHLTDGARLVRKLRKDLGPDAILGAFCGRTRHEGIN
HHHHHHHHHHHCCEEEEECCCCCCEEECHHHHHHHHHHHHCCHHHHHHHHHCCHHHCCCC
AAEAGADYVAFGPVGTTALGDGSLAEAELFGWWSEMIEVPVVAEGALTPEKVAELAPLTD
HHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCHHH
FFAVGEEIWREEDAAAALRRLLAPLG
HHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEVERPQLYLVTPPEIDLEIFPDRLAAVLDSTEVACLRLALAGKDEDRIARTGDALREV
CCCCCCEEEEECCCCCCEEECHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
AHARDVALVIENHVLMVERLGLDGVHLTDGARLVRKLRKDLGPDAILGAFCGRTRHEGIN
HHHHHHHHHHHCCEEEEECCCCCCEEECHHHHHHHHHHHHCCHHHHHHHHHCCHHHCCCC
AAEAGADYVAFGPVGTTALGDGSLAEAELFGWWSEMIEVPVVAEGALTPEKVAELAPLTD
HHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCHHH
FFAVGEEIWREEDAAAALRRLLAPLG
HHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA