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The map label for this gene is ansA [H]

Identifier: 77461848

GI number: 77461848

Start: 6302085

End: 6303092

Strand: Direct

Name: ansA [H]

Synonym: Pfl01_5627

Alternate gene names: 77461848

Gene position: 6302085-6303092 (Clockwise)

Preceding gene: 77461847

Following gene: 77461850

Centisome position: 97.88

GC content: 65.58

Gene sequence:

>1008_bases
ATGAATTCCTCGACCTACCCTGCCGCCCAGCACGTCATGGTGCTGTACACCGGTGGCACCATCGGCATGCAGGCCAGCGC
CAATGGCCTGGCCCCGGCCTCCGGTTTCGAAGCGCGGATGCGCGATTACCTGCACAGCCAGCCGGAGCTGGTGGTGCCGC
AGTGGCGCTTTCGCGAGATGTCGCCGCTGATCGACAGCGCCAACATGACCCCGGCTTACTGGCAGCAACTGCGTGAAGCG
GTGGTCGACGCCGTCGATGTGCAAGGTTGCGACAGCGTGCTGATCCTGCACGGCACCGACACCCTGGCCTACAGCGCCGC
CGCCATGAGCTTCCAGTTGCTCGGCCTGCACGCCCGCGTGTGCTTCACCGGCTCGATGCTGCCGGCCGGCGTCACCGACA
GCGACGCCTGGGAAAACCTCAGCGGCGCACTGGTCGCCCTCGGCCACGGCCTGGCGCCGGGCGTGCACCTGTACTTCCAC
GGCGAGCTGCTGGCGCCGACCCGTTGCGCAAAAGTGCGCAGCTTCGGCCGTCACCCGTTCAAACGCCTGGAGCGCCAGGG
TGGCGGCGTGAAAGCCACTTCCCTGCCAGCAGCGTTGAACTACAACCAACCGAAGCAACTGGCAAAAGTTGCCGTGTTGC
CGCTGTTCCCGGGCATTGGCGCCGAGATCATCGACGGCCTGCTCGACAGCGGCATTCAGGGTCTGGTGCTGGAGTGCTAC
GGCAGCGGCACCGGGCCGAGCGACAACCCGCAATTCCTCGCCAGCCTCGGCCGGGCGCGGGACAGCGGCGTGGTCGTGGT
GGCGGTCACGCAGTGCCATGAAGGCGGTGTGGAACTGGATGTGTATGAAGCCGGCAGCCGCTTGCGCGGTGTTGGCGTGT
TGTCCGGTGGCGGCATGACTCGCGAGGCAGCGTTCGGCAAGTTACACGGGTTGCTGGGGGCAGGACTCGAAGTAACAAAC
ATTCGGCAGCTTGTGGAACTGGATCTCTGTGGAGAACTGTCTTTGTAA

Upstream 100 bases:

>100_bases
ATCGGGTAAAAACCGGGCGCAGATCCTGCGCCCGGCGTGACACAAGCAGCGGTCGGCGTCATGCTCGACCGCATGCTGTC
CTCGTTCAGGAGAACTCCCC

Downstream 100 bases:

>100_bases
CTTGGTTCGTTCTGCAGTCAACTGATCAATGACGATCAAAAATACCCCTCCTTACTGCCAATGGAGGGGTATCAACTATT
TCACAGACTGAACAATAAAG

Product: asparaginase

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNSSTYPAAQHVMVLYTGGTIGMQASANGLAPASGFEARMRDYLHSQPELVVPQWRFREMSPLIDSANMTPAYWQQLREA
VVDAVDVQGCDSVLILHGTDTLAYSAAAMSFQLLGLHARVCFTGSMLPAGVTDSDAWENLSGALVALGHGLAPGVHLYFH
GELLAPTRCAKVRSFGRHPFKRLERQGGGVKATSLPAALNYNQPKQLAKVAVLPLFPGIGAEIIDGLLDSGIQGLVLECY
GSGTGPSDNPQFLASLGRARDSGVVVVAVTQCHEGGVELDVYEAGSRLRGVGVLSGGGMTREAAFGKLHGLLGAGLEVTN
IRQLVELDLCGELSL

Sequences:

>Translated_335_residues
MNSSTYPAAQHVMVLYTGGTIGMQASANGLAPASGFEARMRDYLHSQPELVVPQWRFREMSPLIDSANMTPAYWQQLREA
VVDAVDVQGCDSVLILHGTDTLAYSAAAMSFQLLGLHARVCFTGSMLPAGVTDSDAWENLSGALVALGHGLAPGVHLYFH
GELLAPTRCAKVRSFGRHPFKRLERQGGGVKATSLPAALNYNQPKQLAKVAVLPLFPGIGAEIIDGLLDSGIQGLVLECY
GSGTGPSDNPQFLASLGRARDSGVVVVAVTQCHEGGVELDVYEAGSRLRGVGVLSGGGMTREAAFGKLHGLLGAGLEVTN
IRQLVELDLCGELSL
>Mature_335_residues
MNSSTYPAAQHVMVLYTGGTIGMQASANGLAPASGFEARMRDYLHSQPELVVPQWRFREMSPLIDSANMTPAYWQQLREA
VVDAVDVQGCDSVLILHGTDTLAYSAAAMSFQLLGLHARVCFTGSMLPAGVTDSDAWENLSGALVALGHGLAPGVHLYFH
GELLAPTRCAKVRSFGRHPFKRLERQGGGVKATSLPAALNYNQPKQLAKVAVLPLFPGIGAEIIDGLLDSGIQGLVLECY
GSGTGPSDNPQFLASLGRARDSGVVVVAVTQCHEGGVELDVYEAGSRLRGVGVLSGGGMTREAAFGKLHGLLGAGLEVTN
IRQLVELDLCGELSL

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=368, Percent_Identity=32.3369565217391, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1788065, Length=337, Percent_Identity=35.6083086053413, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI193207372, Length=362, Percent_Identity=29.8342541436464, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI193207374, Length=361, Percent_Identity=29.9168975069252, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17558278, Length=361, Percent_Identity=29.9168975069252, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17558280, Length=361, Percent_Identity=29.9168975069252, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24645530, Length=357, Percent_Identity=33.3333333333333, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24639609, Length=371, Percent_Identity=31.8059299191375, Blast_Score=147, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35311; Mature: 35311

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSTYPAAQHVMVLYTGGTIGMQASANGLAPASGFEARMRDYLHSQPELVVPQWRFREM
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCEECCCHHHHHH
SPLIDSANMTPAYWQQLREAVVDAVDVQGCDSVLILHGTDTLAYSAAAMSFQLLGLHARV
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEE
CFTGSMLPAGVTDSDAWENLSGALVALGHGLAPGVHLYFHGELLAPTRCAKVRSFGRHPF
EEECCCCCCCCCCCHHHHHHCCHHEECCCCCCCCEEEEEECCEECCHHHHHHHHHCCCHH
KRLERQGGGVKATSLPAALNYNQPKQLAKVAVLPLFPGIGAEIIDGLLDSGIQGLVLECY
HHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEE
GSGTGPSDNPQFLASLGRARDSGVVVVAVTQCHEGGVELDVYEAGSRLRGVGVLSGGGMT
CCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCC
REAAFGKLHGLLGAGLEVTNIRQLVELDLCGELSL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNSSTYPAAQHVMVLYTGGTIGMQASANGLAPASGFEARMRDYLHSQPELVVPQWRFREM
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCEECCCHHHHHH
SPLIDSANMTPAYWQQLREAVVDAVDVQGCDSVLILHGTDTLAYSAAAMSFQLLGLHARV
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEE
CFTGSMLPAGVTDSDAWENLSGALVALGHGLAPGVHLYFHGELLAPTRCAKVRSFGRHPF
EEECCCCCCCCCCCHHHHHHCCHHEECCCCCCCCEEEEEECCEECCHHHHHHHHHCCCHH
KRLERQGGGVKATSLPAALNYNQPKQLAKVAVLPLFPGIGAEIIDGLLDSGIQGLVLECY
HHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEE
GSGTGPSDNPQFLASLGRARDSGVVVVAVTQCHEGGVELDVYEAGSRLRGVGVLSGGGMT
CCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCC
REAAFGKLHGLLGAGLEVTNIRQLVELDLCGELSL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]