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The map label for this gene is rep [H]

Identifier: 77461740

GI number: 77461740

Start: 6187561

End: 6189570

Strand: Direct

Name: rep [H]

Synonym: Pfl01_5519

Alternate gene names: 77461740

Gene position: 6187561-6189570 (Clockwise)

Preceding gene: 77461738

Following gene: 77461741

Centisome position: 96.1

GC content: 60.6

Gene sequence:

>2010_bases
ATGTCCCGACTCAATCCCCGGCAGCAAGAAGCCGTGAACTACGTCGGCGGCCCTCTATTGGTGCTCGCCGGTGCTGGCTC
CGGCAAGACCAGCGTGATCACGCGCAAGATTGCGCATCTGATCCAGAACTGCGGCATCCGCGCCCAGTACATCGTCGCCA
TGACCTTCACCAACAAGGCCGCGCGCGAGATGAAGGAGCGGGTCGGCACCCTGCTGCGTCCAGGTGAAGGACGCGGGCTG
ACGGTCTGCACCTTCCACAACCTGGGCCTGAACATCATCCGCAAGGAACATGAACGGCTGGGCTACAAACCCGGTTTCTC
GATCTTCGACGAAACCGACGTCAAGTCGCTGATGACCGACATCATGCAGAAGGAATATGCAGGCGACGACGGCGTCGACG
AGATCAAGAACATGATCGGCGCCTGGAAAAACGACCTGATTCTGCCGGCCCAGGCCCTGGAAAACGCCCGCAACCCCAAG
GAACAGACCGCCGCCATCGTCTACACCCACTACCAGCGCACGCTCAAGGCGTTCAACGCGGTGGACTTCGACGACCTGAT
CCTGCTGCCGGTGAAACTCTTCGAAGAGCACGCCGACATCCTCGAAAAGTGGCAGAACAAGGTGCGTTACCTGCTGGTCG
ACGAATACCAGGACACCAACGCCAGCCAGTACCTGCTGGTGAAAATGCTCATCGGCAAACGCAACCAGTTCACCGTGGTG
GGCGACGACGACCAGTCGATCTATGCCTGGCGCGGCGCGCGGCCGGAAAACCTGATGCTGCTCAAGGACGACTATCCGTC
CCTGAAAGTGGTGATGCTCGAGCAGAACTACCGCTCCACCAGCCGCATCCTGCGCTGCGCCAACGTGCTGATCTCGAACA
ACCCGCACGAATTCGAAAAACAGCTGTGGAGTGAGATGGGCCACGGCGACGAAATCCGCGTGATCCGCTGCCGCAACGAG
GACGCCGAAGCCGAGCGCGTGGCCATGGAAATCCTCAGCCTGCACTTGCGCACCGACCGCCCGTACAGCGATTTCGCGAT
CCTCTATCGCGGCAACTACCAGGCCAAGCTGATCGAGCTGAAACTGCAGCACCATCAGGTGCCGTACCGTTTGAGCGGCG
GCAACAGCTTCTTCGGCCGTCAGGAAGTGAAAGACCTGATGGCCTACTTCCGCCTGATCGTGAACCCGGACGACGACAAC
GCCTTCCTGCGGGTGATCAACGTGCCGCGCCGAGAAATCGGCTCGACGACCCTGGAAAAACTCGGCAACTATGCCACCGA
ACGCAAGATCTCGATGTACGCCGCCACCGATGAACTCGGCCTGGGCGAGCATCTGGACAGCCGCTTCACCGACCGCCTGT
CGCGCTTCAAGCGCTTCATGGACAAGGTGCGCGAGCAGTGCGCCGGCGAAGACCCGATCTCCGCCCTGCGCAGCATGGTC
ATGGACATCGACTACGAGAACTGGCTGCGCACCAACAGTTCCAGCGACAAGGCCGCGGACTACCGGATGAGCAACGTCTG
GTTCCTGATCGAAGCCTTGAAGAACACCCTGGAAAAAGACGAAGACGGCGAAATGACCGTCGAGGACGCCATCGGCAAAC
TCGTCCTGCGTGACATGCTGGAACGTCAGCAGGAAGAGGAAGAGGGTGCCGAAGGCGTGCAGATGATGACCCTGCATGCG
TCCAAGGGCCTGGAATTCCCTTACGTGTTCATCATGGGCATGGAAGAGGAAATCCTCCCGCACCGCTCCAGCATCGAAGC
CGACACCATCGAAGAAGAACGCCGCCTGGCTTACGTGGGCATCACCCGCGCGCGCCAGACCCTGGCGTTTACCTTCGCCG
CCAAGCGCAAACAGTACGGCGAGATCATCGATTGCGCGCCAAGCCGCTTCCTCGATGAACTGCCGCCGGACGACCTGGCC
TGGGAAGGCAACGACGACACACCGACCGAAGTCAAAGCCGTGCGGGGCAACAGCGCACTGGCTGATATACGCGCGATGTT
AAAGCGCTAG

Upstream 100 bases:

>100_bases
ATTTGAATCGCGTGCTGCGTCACAAGTCGGAGAGAGCAGCACAAATCGCTGAGCCTGTTAAAATGCCGGCCCATTTCGTC
AACGACTCCCGAATTTTCGT

Downstream 100 bases:

>100_bases
AATTGACCACTTTTTACTAGACCTTCGGCGCACAAAGCGCCAAATGAGGACAGCTTCATGGAAGCATTGCACCAGAAAAT
CCGCGAACAAGGCATTGTGC

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKAAREMKERVGTLLRPGEGRGL
TVCTFHNLGLNIIRKEHERLGYKPGFSIFDETDVKSLMTDIMQKEYAGDDGVDEIKNMIGAWKNDLILPAQALENARNPK
EQTAAIVYTHYQRTLKAFNAVDFDDLILLPVKLFEEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKMLIGKRNQFTVV
GDDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHEFEKQLWSEMGHGDEIRVIRCRNE
DAEAERVAMEILSLHLRTDRPYSDFAILYRGNYQAKLIELKLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLIVNPDDDN
AFLRVINVPRREIGSTTLEKLGNYATERKISMYAATDELGLGEHLDSRFTDRLSRFKRFMDKVREQCAGEDPISALRSMV
MDIDYENWLRTNSSSDKAADYRMSNVWFLIEALKNTLEKDEDGEMTVEDAIGKLVLRDMLERQQEEEEGAEGVQMMTLHA
SKGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVGITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLA
WEGNDDTPTEVKAVRGNSALADIRAMLKR

Sequences:

>Translated_669_residues
MSRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKAAREMKERVGTLLRPGEGRGL
TVCTFHNLGLNIIRKEHERLGYKPGFSIFDETDVKSLMTDIMQKEYAGDDGVDEIKNMIGAWKNDLILPAQALENARNPK
EQTAAIVYTHYQRTLKAFNAVDFDDLILLPVKLFEEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKMLIGKRNQFTVV
GDDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHEFEKQLWSEMGHGDEIRVIRCRNE
DAEAERVAMEILSLHLRTDRPYSDFAILYRGNYQAKLIELKLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLIVNPDDDN
AFLRVINVPRREIGSTTLEKLGNYATERKISMYAATDELGLGEHLDSRFTDRLSRFKRFMDKVREQCAGEDPISALRSMV
MDIDYENWLRTNSSSDKAADYRMSNVWFLIEALKNTLEKDEDGEMTVEDAIGKLVLRDMLERQQEEEEGAEGVQMMTLHA
SKGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVGITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLA
WEGNDDTPTEVKAVRGNSALADIRAMLKR
>Mature_668_residues
SRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKAAREMKERVGTLLRPGEGRGLT
VCTFHNLGLNIIRKEHERLGYKPGFSIFDETDVKSLMTDIMQKEYAGDDGVDEIKNMIGAWKNDLILPAQALENARNPKE
QTAAIVYTHYQRTLKAFNAVDFDDLILLPVKLFEEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKMLIGKRNQFTVVG
DDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHEFEKQLWSEMGHGDEIRVIRCRNED
AEAERVAMEILSLHLRTDRPYSDFAILYRGNYQAKLIELKLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLIVNPDDDNA
FLRVINVPRREIGSTTLEKLGNYATERKISMYAATDELGLGEHLDSRFTDRLSRFKRFMDKVREQCAGEDPISALRSMVM
DIDYENWLRTNSSSDKAADYRMSNVWFLIEALKNTLEKDEDGEMTVEDAIGKLVLRDMLERQQEEEEGAEGVQMMTLHAS
KGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVGITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLAW
EGNDDTPTEVKAVRGNSALADIRAMLKR

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=669, Percent_Identity=53.5127055306427, Blast_Score=710, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=644, Percent_Identity=38.0434782608696, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=356, Percent_Identity=28.6516853932584, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=461, Percent_Identity=31.4533622559653, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324477, Length=664, Percent_Identity=22.8915662650602, Blast_Score=104, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76672; Mature: 76541

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKA
CCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCHH
AREMKERVGTLLRPGEGRGLTVCTFHNLGLNIIRKEHERLGYKPGFSIFDETDVKSLMTD
HHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
IMQKEYAGDDGVDEIKNMIGAWKNDLILPAQALENARNPKEQTAAIVYTHYQRTLKAFNA
HHHHHCCCCCCHHHHHHHHHCCCCCCEECHHHHHCCCCCCHHHHEEEHHHHHHHHHHHCC
VDFDDLILLPVKLFEEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKMLIGKRNQFTVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHEFEK
ECCCCCEEEECCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHH
QLWSEMGHGDEIRVIRCRNEDAEAERVAMEILSLHLRTDRPYSDFAILYRGNYQAKLIEL
HHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEE
KLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLIVNPDDDNAFLRVINVPRREIGSTTLEK
EHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEECCHHHHCHHHHHH
LGNYATERKISMYAATDELGLGEHLDSRFTDRLSRFKRFMDKVREQCAGEDPISALRSMV
HCCHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MDIDYENWLRTNSSSDKAADYRMSNVWFLIEALKNTLEKDEDGEMTVEDAIGKLVLRDML
HHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHH
ERQQEEEEGAEGVQMMTLHASKGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVG
HHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCHHHHHHHHHEEEEE
ITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLAWEGNDDTPTEVKAVRGNSAL
HHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHH
ADIRAMLKR
HHHHHHHCC
>Mature Secondary Structure 
SRLNPRQQEAVNYVGGPLLVLAGAGSGKTSVITRKIAHLIQNCGIRAQYIVAMTFTNKA
CCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCHH
AREMKERVGTLLRPGEGRGLTVCTFHNLGLNIIRKEHERLGYKPGFSIFDETDVKSLMTD
HHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
IMQKEYAGDDGVDEIKNMIGAWKNDLILPAQALENARNPKEQTAAIVYTHYQRTLKAFNA
HHHHHCCCCCCHHHHHHHHHCCCCCCEECHHHHHCCCCCCHHHHEEEHHHHHHHHHHHCC
VDFDDLILLPVKLFEEHADILEKWQNKVRYLLVDEYQDTNASQYLLVKMLIGKRNQFTVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
GDDDQSIYAWRGARPENLMLLKDDYPSLKVVMLEQNYRSTSRILRCANVLISNNPHEFEK
ECCCCCEEEECCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHH
QLWSEMGHGDEIRVIRCRNEDAEAERVAMEILSLHLRTDRPYSDFAILYRGNYQAKLIEL
HHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEE
KLQHHQVPYRLSGGNSFFGRQEVKDLMAYFRLIVNPDDDNAFLRVINVPRREIGSTTLEK
EHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEECCHHHHCHHHHHH
LGNYATERKISMYAATDELGLGEHLDSRFTDRLSRFKRFMDKVREQCAGEDPISALRSMV
HCCHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MDIDYENWLRTNSSSDKAADYRMSNVWFLIEALKNTLEKDEDGEMTVEDAIGKLVLRDML
HHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHH
ERQQEEEEGAEGVQMMTLHASKGLEFPYVFIMGMEEEILPHRSSIEADTIEEERRLAYVG
HHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCHHHHHHHHHEEEEE
ITRARQTLAFTFAAKRKQYGEIIDCAPSRFLDELPPDDLAWEGNDDTPTEVKAVRGNSAL
HHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHH
ADIRAMLKR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]