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The map label for this gene is gcvT [H]

Identifier: 77461649

GI number: 77461649

Start: 6094701

End: 6095783

Strand: Reverse

Name: gcvT [H]

Synonym: Pfl01_5428

Alternate gene names: 77461649

Gene position: 6095783-6094701 (Counterclockwise)

Preceding gene: 77461650

Following gene: 77461648

Centisome position: 94.68

GC content: 62.14

Gene sequence:

>1083_bases
ATGGGACAGCGTACGCCTCTGTATGACCTGCATCTCGCCCTCGGCGCGAAGATGGTCGATTTTGGCGGTTGGGACATGCC
CCTGCATTACGGCTCGCAGGTCGAGGAGCACCACGAGGTGCGTCGCGATTGCGGGGTGTTCGATGTTTCCCACATGACCG
TGATCGATGTCACCGGCCCTCAGGCCAAGGCCTGGCTTCAGCATTTGCTGGCCAATGACGTCGAACGCCTGCACAGCCCC
GGCCGTGCGTTGTACAGCACCATGCTCAACGAGCGCGGCGGTATCGTCGACGACATGATCGTCTACCGCCTTGAGGACGG
TTATCGTCTGGTGGTCAACGCCTCCACCCGCGATCAGGACCTGGCCTGGATGCAGGCACAATTGGGCAGCTTCGATGTTC
AGATGACCGAACGCTCCGAGCTGGCGATGCTCGCCATTCAAGGTCCCCATGCCCGGCACAGGATTGCCGAGCTGGTGACC
CAGTCCCGCGCCAACCTGATCCAGATGCTCAAGCCTTTCGAAGGCCACGTCGACGGCGACTGGTTCATTGCGCGCACCGG
CTACACCGGTGAGGACGGTCTGGAAATCGCCCTGCCGGCCGATCAGGCCCCCGGATTCTTCAACGATCTGGTGGGCGCCG
GCATTTCCCCCATCGGCCTCGGTGCCCGCGACACGCTGCGGGTCGAGGCCGGCATGAACCTCTACGGTCAGGACATCCAC
CAGGACGTTTCGCCTCTGGCCTCGAACATGGCCTGGAGCATCGCCTGGGAGCCGGCAACGCGTCGGTTCATCGGTCGCGA
TGCGCTGGAAGCGGAAAAAACCGCCGGCGTGCAGCACAAACTGGTCGGCCTGGTGCTTGAAGAGCGCGGGGTTTTGCGGG
CCCATCAGGTCGTTCGTATCGCTGATGTTGGCGAAGGGGAGATCACCAGTGGTAGTTTCTCTCCTACGCTTAGCAAGTCG
ATTGCCCTGGCGCGTGTACCGATGGCGACTGCCGACCGCGCCGAAGTGGAAATCCGTGGCAAGTGGTATCCGGTACGCGT
GGTCAAACCGACCTTTGTCCGTCACGGCAAAACCTTGATCTAA

Upstream 100 bases:

>100_bases
AGACCCTGTCGCTGAAATCGGCTGAAAGCCCTTTTTTACAGGGTTTTCCGAACCGTGCAGCAGCGCTCCGCACCTTCGCC
ACGCCCGGAAGGAGAAACCC

Downstream 100 bases:

>100_bases
ACCTTTTCCGGCGGGCACGACCGCTGACAATTTCTTGAGGACACCGAGCATGAGCAACATCCCAGCTGAACTGCGTTTTG
CCGAAAGTCATGAATGGGCG

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MGQRTPLYDLHLALGAKMVDFGGWDMPLHYGSQVEEHHEVRRDCGVFDVSHMTVIDVTGPQAKAWLQHLLANDVERLHSP
GRALYSTMLNERGGIVDDMIVYRLEDGYRLVVNASTRDQDLAWMQAQLGSFDVQMTERSELAMLAIQGPHARHRIAELVT
QSRANLIQMLKPFEGHVDGDWFIARTGYTGEDGLEIALPADQAPGFFNDLVGAGISPIGLGARDTLRVEAGMNLYGQDIH
QDVSPLASNMAWSIAWEPATRRFIGRDALEAEKTAGVQHKLVGLVLEERGVLRAHQVVRIADVGEGEITSGSFSPTLSKS
IALARVPMATADRAEVEIRGKWYPVRVVKPTFVRHGKTLI

Sequences:

>Translated_360_residues
MGQRTPLYDLHLALGAKMVDFGGWDMPLHYGSQVEEHHEVRRDCGVFDVSHMTVIDVTGPQAKAWLQHLLANDVERLHSP
GRALYSTMLNERGGIVDDMIVYRLEDGYRLVVNASTRDQDLAWMQAQLGSFDVQMTERSELAMLAIQGPHARHRIAELVT
QSRANLIQMLKPFEGHVDGDWFIARTGYTGEDGLEIALPADQAPGFFNDLVGAGISPIGLGARDTLRVEAGMNLYGQDIH
QDVSPLASNMAWSIAWEPATRRFIGRDALEAEKTAGVQHKLVGLVLEERGVLRAHQVVRIADVGEGEITSGSFSPTLSKS
IALARVPMATADRAEVEIRGKWYPVRVVKPTFVRHGKTLI
>Mature_359_residues
GQRTPLYDLHLALGAKMVDFGGWDMPLHYGSQVEEHHEVRRDCGVFDVSHMTVIDVTGPQAKAWLQHLLANDVERLHSPG
RALYSTMLNERGGIVDDMIVYRLEDGYRLVVNASTRDQDLAWMQAQLGSFDVQMTERSELAMLAIQGPHARHRIAELVTQ
SRANLIQMLKPFEGHVDGDWFIARTGYTGEDGLEIALPADQAPGFFNDLVGAGISPIGLGARDTLRVEAGMNLYGQDIHQ
DVSPLASNMAWSIAWEPATRRFIGRDALEAEKTAGVQHKLVGLVLEERGVLRAHQVVRIADVGEGEITSGSFSPTLSKSI
ALARVPMATADRAEVEIRGKWYPVRVVKPTFVRHGKTLI

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=369, Percent_Identity=34.6883468834688, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI257796258, Length=340, Percent_Identity=35.2941176470588, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI257796254, Length=364, Percent_Identity=30.7692307692308, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI257796256, Length=305, Percent_Identity=32.4590163934426, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI24797151, Length=333, Percent_Identity=27.3273273273273, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI197927446, Length=362, Percent_Identity=26.2430939226519, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI21361378, Length=362, Percent_Identity=26.2430939226519, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI194306651, Length=383, Percent_Identity=24.8041775456919, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789272, Length=364, Percent_Identity=55.2197802197802, Blast_Score=410, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17560118, Length=371, Percent_Identity=32.3450134770889, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71994045, Length=325, Percent_Identity=26.4615384615385, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71994052, Length=325, Percent_Identity=26.4615384615385, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320222, Length=383, Percent_Identity=32.6370757180157, Blast_Score=169, Evalue=7e-43,
Organism=Drosophila melanogaster, GI20129441, Length=375, Percent_Identity=34.4, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI20130091, Length=368, Percent_Identity=24.1847826086957, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571104, Length=315, Percent_Identity=25.3968253968254, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 39804; Mature: 39673

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQRTPLYDLHLALGAKMVDFGGWDMPLHYGSQVEEHHEVRRDCGVFDVSHMTVIDVTGP
CCCCCCCEEEHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEECCEEEEEECCC
QAKAWLQHLLANDVERLHSPGRALYSTMLNERGGIVDDMIVYRLEDGYRLVVNASTRDQD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHEEEEEECCCEEEEEECCCCCCH
LAWMQAQLGSFDVQMTERSELAMLAIQGPHARHRIAELVTQSRANLIQMLKPFEGHVDGD
HHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WFIARTGYTGEDGLEIALPADQAPGFFNDLVGAGISPIGLGARDTLRVEAGMNLYGQDIH
EEEEECCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHH
QDVSPLASNMAWSIAWEPATRRFIGRDALEAEKTAGVQHKLVGLVLEERGVLRAHQVVRI
HHHHHHHHCCEEEEEECHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHEEEE
ADVGEGEITSGSFSPTLSKSIALARVPMATADRAEVEIRGKWYPVRVVKPTFVRHGKTLI
EECCCCCCCCCCCCCHHHHCCHHEECCCCCCCCCEEEEECCEEEEEEECHHHHCCCCCCC
>Mature Secondary Structure 
GQRTPLYDLHLALGAKMVDFGGWDMPLHYGSQVEEHHEVRRDCGVFDVSHMTVIDVTGP
CCCCCCEEEHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCEEEECCEEEEEECCC
QAKAWLQHLLANDVERLHSPGRALYSTMLNERGGIVDDMIVYRLEDGYRLVVNASTRDQD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHEEEEEECCCEEEEEECCCCCCH
LAWMQAQLGSFDVQMTERSELAMLAIQGPHARHRIAELVTQSRANLIQMLKPFEGHVDGD
HHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
WFIARTGYTGEDGLEIALPADQAPGFFNDLVGAGISPIGLGARDTLRVEAGMNLYGQDIH
EEEEECCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHH
QDVSPLASNMAWSIAWEPATRRFIGRDALEAEKTAGVQHKLVGLVLEERGVLRAHQVVRI
HHHHHHHHCCEEEEEECHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHEEEE
ADVGEGEITSGSFSPTLSKSIALARVPMATADRAEVEIRGKWYPVRVVKPTFVRHGKTLI
EECCCCCCCCCCCCCHHHHCCHHEECCCCCCCCCEEEEECCEEEEEEECHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]