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The map label for this gene is puuA [H]

Identifier: 77461629

GI number: 77461629

Start: 6073024

End: 6074400

Strand: Direct

Name: puuA [H]

Synonym: Pfl01_5408

Alternate gene names: 77461629

Gene position: 6073024-6074400 (Clockwise)

Preceding gene: 77461621

Following gene: 77461630

Centisome position: 94.32

GC content: 59.77

Gene sequence:

>1377_bases
ATGTCGGTACCCCCGCGTGCCGTTCAGCTTAACGAAGCGAACGCGTTCCTTAAGGAACATCCTGAGGTTCTGTACGTTGA
CCTTCTGATTGCGGATATGAATGGTGTGGTGCGCGGCAAGCGCATCGAACGCACCAGCCTCCACAAGGTTTACGAGAAAG
GCATCAACCTGCCGGCCTCTCTATTTGCTCTGGATATCAATGGTTCGACGGTGGAAAGCACCGGCCTGGGCCTGGACATC
GGCGACGCCGACCGTATCTGCTATCCAATCCCCGATACCCTGTGCAACGAGCCATGGCAGAAGCGCCCGACCGCGCAACT
GTTGATGACCATGCACGAACTCGAAGGTGACCCTTTCTTCGCCGACCCGCGCGAAGTGCTCCGTCAAGTTGTTGCAAAGT
TTGACGAGCTTGGCTTGACCATCTGCGCCGCGTTCGAACTCGAGTTTTACCTGATCGACCAGGAAAACGTGAACGGCCGC
CCTCAGCCGCCACGCTCGCCGATCTCCGGCAAACGCCCGCATTCGACTCAGGTTTACCTGATCGACGACCTCGACGAATA
CGTCGACTGCCTCCAGGACATTCTGGAAGGCGCCAAAGAGCAAGGTATTCCGGCCGACGCCATCGTCAAGGAAAGTGCCC
CGGCGCAGTTCGAAGTGAACCTGCACCACGTCGCCGACCCGATCAAGGCATGCGACTACGCGGTCCTGCTCAAGCGTCTG
ATCAAGAACATCGCCTACGACCATGAAATGGACACCACTTTCATGGCCAAGCCGTACCCGGGCCAGGCAGGCAACGGTCT
GCACGTCCACATTTCGGTTCTCGACAAAGAAGGCAAAAACATTTTTGCCAGCGAGGATCCCGAGCAGAACGCCGCGCTGC
GTCACGCGATCGGCGGTGTGCTCGAGACCCTGCCCGCGCAGATGGCTTTCCTCTGCCCGAACGTCAACTCCTACCGTCGT
TTCGGCGCACAGTTCTACGTGCCGAACTCGCCGAGCTGGGGCCTGGACAACCGCACCGTGGCCCTGCGCGTACCGACCGG
CTCCGCCGACGCGGTCCGTCTGGAACACCGCGTTGCCGGCGCCGACGCCAACCCGTACCTGCTGATGGCTTCGGTGCTGG
CGGGCGTGCACCACGGTCTGACCAACAAGATCGAGCCACCGGCTCCGACCGAAGGCAACAGCTACGAGCAGAACGAGCAG
AGCCTGCCGAACAACTTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGGTGATGGCCAAGTACATCGATCCGAAATA
CATCGATATCTTCGTCGCCTGCAAAGAGAGCGAACTGGAGGAGTTCGAACACTCCATCTCCGACCTCGAGTACAACTGGT
ACCTGCACACCGTTTAA

Upstream 100 bases:

>100_bases
AGGGCAGAAAAGGCCTCGCTTGACTTCGGCAGGCCGTTCGGGTTGACTGAAACCAGAAAAGATCAATGATTGATATTTTT
AACAACAAAGGTGTTGCATC

Downstream 100 bases:

>100_bases
GCGGACTGCAGCAAAGGAAAACGCCGTTGGCTTCTCAGCCAGCGGCGTTTTTTTATTGGCCTCTGTGGCAGACATTCAAG
CTCTGCGTACAATGCCCGCT

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDI
GDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFFADPREVLRQVVAKFDELGLTICAAFELEFYLIDQENVNGR
PQPPRSPISGKRPHSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADPIKACDYAVLLKRL
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISVLDKEGKNIFASEDPEQNAALRHAIGGVLETLPAQMAFLCPNVNSYRR
FGAQFYVPNSPSWGLDNRTVALRVPTGSADAVRLEHRVAGADANPYLLMASVLAGVHHGLTNKIEPPAPTEGNSYEQNEQ
SLPNNLRDALRELDDSEVMAKYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV

Sequences:

>Translated_458_residues
MSVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDI
GDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFFADPREVLRQVVAKFDELGLTICAAFELEFYLIDQENVNGR
PQPPRSPISGKRPHSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADPIKACDYAVLLKRL
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISVLDKEGKNIFASEDPEQNAALRHAIGGVLETLPAQMAFLCPNVNSYRR
FGAQFYVPNSPSWGLDNRTVALRVPTGSADAVRLEHRVAGADANPYLLMASVLAGVHHGLTNKIEPPAPTEGNSYEQNEQ
SLPNNLRDALRELDDSEVMAKYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV
>Mature_457_residues
SVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGLDIG
DADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFFADPREVLRQVVAKFDELGLTICAAFELEFYLIDQENVNGRP
QPPRSPISGKRPHSTQVYLIDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADPIKACDYAVLLKRLI
KNIAYDHEMDTTFMAKPYPGQAGNGLHVHISVLDKEGKNIFASEDPEQNAALRHAIGGVLETLPAQMAFLCPNVNSYRRF
GAQFYVPNSPSWGLDNRTVALRVPTGSADAVRLEHRVAGADANPYLLMASVLAGVHHGLTNKIEPPAPTEGNSYEQNEQS
LPNNLRDALRELDDSEVMAKYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=429, Percent_Identity=22.8438228438228, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI87081870, Length=445, Percent_Identity=43.5955056179775, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1790301, Length=480, Percent_Identity=24.7916666666667, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 51025; Mature: 50894

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
CCCCCCEEEECCHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEE
LFALDINGSTVESTGLGLDIGDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFF
EEEEECCCCEEECCCCCEECCCCCCEEECCCHHHCCCCHHCCCHHHHHHHHHHHCCCCCC
ADPREVLRQVVAKFDELGLTICAAFELEFYLIDQENVNGRPQPPRSPISGKRPHSTQVYL
CCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEE
IDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADPIKACDYAVLLKRL
ECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHCHHHHHHHHHHHHHH
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISVLDKEGKNIFASEDPEQNAALRHAIGGV
HHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHH
LETLPAQMAFLCPNVNSYRRFGAQFYVPNSPSWGLDNRTVALRVPTGSADAVRLEHRVAG
HHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEHHHCCC
ADANPYLLMASVLAGVHHGLTNKIEPPAPTEGNSYEQNEQSLPNNLRDALRELDDSEVMA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCHHHHH
KYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV
HHCCCCEEEEEEEECHHHHHHHHHHHHCCEEEEEEECC
>Mature Secondary Structure 
SVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
CCCCCEEEECCHHHHHHCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEE
LFALDINGSTVESTGLGLDIGDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGDPFF
EEEEECCCCEEECCCCCEECCCCCCEEECCCHHHCCCCHHCCCHHHHHHHHHHHCCCCCC
ADPREVLRQVVAKFDELGLTICAAFELEFYLIDQENVNGRPQPPRSPISGKRPHSTQVYL
CCHHHHHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEE
IDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADPIKACDYAVLLKRL
ECCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHCHHHHHHHHHHHHHH
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISVLDKEGKNIFASEDPEQNAALRHAIGGV
HHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHH
LETLPAQMAFLCPNVNSYRRFGAQFYVPNSPSWGLDNRTVALRVPTGSADAVRLEHRVAG
HHHHHHHHHHHCCCCHHHHHCCCEEECCCCCCCCCCCCEEEEEECCCCCCCEEEHHHCCC
ADANPYLLMASVLAGVHHGLTNKIEPPAPTEGNSYEQNEQSLPNNLRDALRELDDSEVMA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCHHHHH
KYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV
HHCCCCEEEEEEEECHHHHHHHHHHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]