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The map label for this gene is pys2 [H]

Identifier: 77461618

GI number: 77461618

Start: 6057026

End: 6059395

Strand: Reverse

Name: pys2 [H]

Synonym: Pfl01_5397

Alternate gene names: 77461618

Gene position: 6059395-6057026 (Counterclockwise)

Preceding gene: 77461620

Following gene: 77461617

Centisome position: 94.11

GC content: 57.68

Gene sequence:

>2370_bases
ATGAGTGATATTGAAAATTATCTTGGACCTCACAAGCAGACCGGGCCGGGCAGATATGAACTGGCGCCAATACCCGTCAA
GGGGGTGACGCCAGTTCGATTGGGACAGGGTGGGTTTTTCTCGGGAAGTTGGGGGTTGAATGAAGGGCCGCATAGAGGGA
CGGGCCAAGGGTCTGGAAAACTTGATCCGTTAACTCCATTCATCTGGAATGATTTGATGCAGGAGCAACAGAATGCTCAG
GCATATATTGATCTTGAGTATGCTGGAGTATTCAGCAATATCGGCGCGGCATCAAATAATGAAGTAGAGAGGGCGAGGCA
AGCAGCCAGTGCTGGACTTTCTGCGGCTCAGAAGGTCAAGGCTGATCAGGAAATAACCATTCAGGTTATTCATTCAAAGT
TGTTGGAGTATCGGGCTCATACCGAGATCGCTTACTCGCTGTATGGACATAATCCATTTTTCCTGATGAAGGATTTGTCC
TTCAAGAAGATACGAGACAGTCTGGCATTGCCGGTTCCGGATGTTTCCGTCGCCTATGCGGCCATCGACAGGGCCTATCG
CTCGGCAATGGAGCTCCGGCGCCTTTCCTGGGTGATGGCCATTATCGCCAATCAACTTCCGGAACTGGCCGCAAGAAGAA
GCCAGGTTGAAGCCGCGACTCAAACCACGAGGGACGCCCAGCAGATTCTTTCTGCCGAAAGGTTATCCGTGGTGAATCTG
GAAACGAATATTCGCCTGCATTTCCTGCCGGGTTTTCTGGTTGAAAGAGTCGCCGCTGCAGCGGGCTCAACCGGAGGCAG
TCTCTCTCAAACACTCACGAATTATAAAATCGCAGCGGACAACATTCGTGCTGTCGAGCAGGCAGCCGTCCGGCCCTATG
CAATCGCCAATCCGGCGATCAACGCCCCGTTGAGCAAGCCTGAGCTCGAAGCGCTGAAGAACCTGGTCGACTTGCAGGCC
ACTATCGATCTGGGCAAGCGCTGGCAGGACTATCACGCTTCACTCCTGCACTCGGAAAGTGCCCGGCATATGGCAGCGGC
GGCGGACGCATTTGCCGGTCTGATTGCCCGCGCCCAAGAGGCGGAGCGTCTGCAGGAGCAGATACGCATTGCCCGGGAGC
TAGAGGCCCGGCATTTGCAGGAGCAGGCGCGTATAGCCGCGGAGGTTGAAGCCAGGCGTGTTGCCGAGGAACAGGCACGT
ATTGCCGCCGAAGCAGAGGCAAGGCGCCTTGCCGAGGAACAAGCGCGCATGGTGGCAGAAGCCATTCGCAACGCCCATAC
CTTCCGCGCGCCAAGTGCCGCTTCGGCGACAGGGCCGTTGTTCATGACTTCAGCTGGAGTCGTGGCAGTTGCCGAGGCTG
CTGCGGCCAGTCTGCAAGCGGCGGTTCGCAGCGCCATCGCCGGGTTGGGCAGTCTTGCGGCCAGTGTCGGGGCTGGCGCG
GTCGTCGGTGTTTCAGCGTTGGTCTACTCGTCGAAACTGGGCAACGGCGAATTGCCGGACCGTTACGCCTTCAGCACGCC
GCTGTCGGACCTCGCGCCCGACTTCGCGCCAGATCTGCACGCCATCGCGGCGGCTGGCGGTACGGTTGACATGCCTTTCC
GCGTCAGTTCAAAGACCGACGCGTACGGGCAGTCAGAGGTTTTTGTCGTCAAGACCGATGGGCGGAATATTCCGTCGGCC
GTCAGGGTGGTGGCGGCTTCCTACGACACCGAACGCAACGTCTACACCGCGACCACCTTCGATGTTCCACCACGCACCCT
GACGTGGACCCCGATCGTCAATCCGGGAAACAGCTCGACGACCTCACCGGCCGGGCAGCCGGATGTGCCTGCCTATACTG
GGGCGACGGTAGTCCCGGTAGAGGGACGCCTCGATTCGTTCCCGGGCGTTTTCGAAGCCGGATTCGATGACTATGTTCTG
GTGTTCCCGCAGGACTCCGGGCTGCCACCGAACTACACGATGTTTCGGGATCGGCGGGAGGATCCGGGTGTCGCATCAGG
TGTGGGGCAGGCGGTATCGGGCAACTGGCTCGGAAGCGCATCGCAGGGCGAGGGCGCGCCGGTGCCGGCGCAGATTGCCG
ATCAGTTGCGAGGGCGGGAGTTCAGGAATTTTCGGGCGTTTCGGGAGGCGTTTTGGAAGGCGGTGGCCGATGATTCTGAG
CTAGTAAGTCAATTTAACAATAGTAGTGCTTTTGAAATGAAAAATGGTCGAGCACCGTTCGTTCGAAAAATTGATAGGGT
GGGAGGGCAAGTGAAGTTTGAGCTCCATCATTCCGTCTATATATCTAGAGGAGGGGAGGTTATGGATGTAGATAACGTTC
GAGTAGTTACTCCTAAACAGCACGGCGCGATACATTCGGAGGGGGATTGA

Upstream 100 bases:

>100_bases
GTTTAACGGGGCGGGCCTATAAAAGACCTCGAGAAAAAGCAGAGGAATAAAACGAATTTTGCGAATTCGATACATGGAAT
AACTGACATGGAGAAGTCGT

Downstream 100 bases:

>100_bases
TATGAGCGCAAAAGAGTTCGAGGACTACACTGAGGAAGATTTTTTGTTGTTTGTTAAAAAAATCTATAATTCTTCGTATT
CGACGGAAGAGCAGCAAATA

Product: S-type Pyocin

Products: NA

Alternate protein names: Killer protein [H]

Number of amino acids: Translated: 789; Mature: 788

Protein sequence:

>789_residues
MSDIENYLGPHKQTGPGRYELAPIPVKGVTPVRLGQGGFFSGSWGLNEGPHRGTGQGSGKLDPLTPFIWNDLMQEQQNAQ
AYIDLEYAGVFSNIGAASNNEVERARQAASAGLSAAQKVKADQEITIQVIHSKLLEYRAHTEIAYSLYGHNPFFLMKDLS
FKKIRDSLALPVPDVSVAYAAIDRAYRSAMELRRLSWVMAIIANQLPELAARRSQVEAATQTTRDAQQILSAERLSVVNL
ETNIRLHFLPGFLVERVAAAAGSTGGSLSQTLTNYKIAADNIRAVEQAAVRPYAIANPAINAPLSKPELEALKNLVDLQA
TIDLGKRWQDYHASLLHSESARHMAAAADAFAGLIARAQEAERLQEQIRIARELEARHLQEQARIAAEVEARRVAEEQAR
IAAEAEARRLAEEQARMVAEAIRNAHTFRAPSAASATGPLFMTSAGVVAVAEAAAASLQAAVRSAIAGLGSLAASVGAGA
VVGVSALVYSSKLGNGELPDRYAFSTPLSDLAPDFAPDLHAIAAAGGTVDMPFRVSSKTDAYGQSEVFVVKTDGRNIPSA
VRVVAASYDTERNVYTATTFDVPPRTLTWTPIVNPGNSSTTSPAGQPDVPAYTGATVVPVEGRLDSFPGVFEAGFDDYVL
VFPQDSGLPPNYTMFRDRREDPGVASGVGQAVSGNWLGSASQGEGAPVPAQIADQLRGREFRNFRAFREAFWKAVADDSE
LVSQFNNSSAFEMKNGRAPFVRKIDRVGGQVKFELHHSVYISRGGEVMDVDNVRVVTPKQHGAIHSEGD

Sequences:

>Translated_789_residues
MSDIENYLGPHKQTGPGRYELAPIPVKGVTPVRLGQGGFFSGSWGLNEGPHRGTGQGSGKLDPLTPFIWNDLMQEQQNAQ
AYIDLEYAGVFSNIGAASNNEVERARQAASAGLSAAQKVKADQEITIQVIHSKLLEYRAHTEIAYSLYGHNPFFLMKDLS
FKKIRDSLALPVPDVSVAYAAIDRAYRSAMELRRLSWVMAIIANQLPELAARRSQVEAATQTTRDAQQILSAERLSVVNL
ETNIRLHFLPGFLVERVAAAAGSTGGSLSQTLTNYKIAADNIRAVEQAAVRPYAIANPAINAPLSKPELEALKNLVDLQA
TIDLGKRWQDYHASLLHSESARHMAAAADAFAGLIARAQEAERLQEQIRIARELEARHLQEQARIAAEVEARRVAEEQAR
IAAEAEARRLAEEQARMVAEAIRNAHTFRAPSAASATGPLFMTSAGVVAVAEAAAASLQAAVRSAIAGLGSLAASVGAGA
VVGVSALVYSSKLGNGELPDRYAFSTPLSDLAPDFAPDLHAIAAAGGTVDMPFRVSSKTDAYGQSEVFVVKTDGRNIPSA
VRVVAASYDTERNVYTATTFDVPPRTLTWTPIVNPGNSSTTSPAGQPDVPAYTGATVVPVEGRLDSFPGVFEAGFDDYVL
VFPQDSGLPPNYTMFRDRREDPGVASGVGQAVSGNWLGSASQGEGAPVPAQIADQLRGREFRNFRAFREAFWKAVADDSE
LVSQFNNSSAFEMKNGRAPFVRKIDRVGGQVKFELHHSVYISRGGEVMDVDNVRVVTPKQHGAIHSEGD
>Mature_788_residues
SDIENYLGPHKQTGPGRYELAPIPVKGVTPVRLGQGGFFSGSWGLNEGPHRGTGQGSGKLDPLTPFIWNDLMQEQQNAQA
YIDLEYAGVFSNIGAASNNEVERARQAASAGLSAAQKVKADQEITIQVIHSKLLEYRAHTEIAYSLYGHNPFFLMKDLSF
KKIRDSLALPVPDVSVAYAAIDRAYRSAMELRRLSWVMAIIANQLPELAARRSQVEAATQTTRDAQQILSAERLSVVNLE
TNIRLHFLPGFLVERVAAAAGSTGGSLSQTLTNYKIAADNIRAVEQAAVRPYAIANPAINAPLSKPELEALKNLVDLQAT
IDLGKRWQDYHASLLHSESARHMAAAADAFAGLIARAQEAERLQEQIRIARELEARHLQEQARIAAEVEARRVAEEQARI
AAEAEARRLAEEQARMVAEAIRNAHTFRAPSAASATGPLFMTSAGVVAVAEAAAASLQAAVRSAIAGLGSLAASVGAGAV
VGVSALVYSSKLGNGELPDRYAFSTPLSDLAPDFAPDLHAIAAAGGTVDMPFRVSSKTDAYGQSEVFVVKTDGRNIPSAV
RVVAASYDTERNVYTATTFDVPPRTLTWTPIVNPGNSSTTSPAGQPDVPAYTGATVVPVEGRLDSFPGVFEAGFDDYVLV
FPQDSGLPPNYTMFRDRREDPGVASGVGQAVSGNWLGSASQGEGAPVPAQIADQLRGREFRNFRAFREAFWKAVADDSEL
VSQFNNSSAFEMKNGRAPFVRKIDRVGGQVKFELHHSVYISRGGEVMDVDNVRVVTPKQHGAIHSEGD

Specific function: Causes breakdown of chromosomal DNA as well as complete inhibition of lipid synthesis in sensitive cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicin/pyosin nuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR003060
- InterPro:   IPR010715
- InterPro:   IPR016128 [H]

Pfam domain/function: PF06958 Pyocin_S [H]

EC number: NA

Molecular weight: Translated: 84780; Mature: 84649

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIENYLGPHKQTGPGRYELAPIPVKGVTPVRLGQGGFFSGSWGLNEGPHRGTGQGSGK
CCCHHHHCCCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LDPLTPFIWNDLMQEQQNAQAYIDLEYAGVFSNIGAASNNEVERARQAASAGLSAAQKVK
CCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
ADQEITIQVIHSKLLEYRAHTEIAYSLYGHNPFFLMKDLSFKKIRDSLALPVPDVSVAYA
CCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHHHHHCCCCCCHHHHHH
AIDRAYRSAMELRRLSWVMAIIANQLPELAARRSQVEAATQTTRDAQQILSAERLSVVNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
ETNIRLHFLPGFLVERVAAAAGSTGGSLSQTLTNYKIAADNIRAVEQAAVRPYAIANPAI
ECCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCEEEHHHHHHHHHHHCCCEEECCCCC
NAPLSKPELEALKNLVDLQATIDLGKRWQDYHASLLHSESARHMAAAADAFAGLIARAQE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERLQEQIRIARELEARHLQEQARIAAEVEARRVAEEQARIAAEAEARRLAEEQARMVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIRNAHTFRAPSAASATGPLFMTSAGVVAVAEAAAASLQAAVRSAIAGLGSLAASVGAGA
HHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VVGVSALVYSSKLGNGELPDRYAFSTPLSDLAPDFAPDLHAIAAAGGTVDMPFRVSSKTD
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCEEECCCCC
AYGQSEVFVVKTDGRNIPSAVRVVAASYDTERNVYTATTFDVPPRTLTWTPIVNPGNSST
CCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCC
TSPAGQPDVPAYTGATVVPVEGRLDSFPGVFEAGFDDYVLVFPQDSGLPPNYTMFRDRRE
CCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCHHHHCCCC
DPGVASGVGQAVSGNWLGSASQGEGAPVPAQIADQLRGREFRNFRAFREAFWKAVADDSE
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
LVSQFNNSSAFEMKNGRAPFVRKIDRVGGQVKFELHHSVYISRGGEVMDVDNVRVVTPKQ
HHHHHCCCCCEEECCCCCHHHHHHHHCCCEEEEEEEEEEEEECCCCEEECCCEEEECCCC
HGAIHSEGD
CCCCCCCCC
>Mature Secondary Structure 
SDIENYLGPHKQTGPGRYELAPIPVKGVTPVRLGQGGFFSGSWGLNEGPHRGTGQGSGK
CCHHHHCCCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LDPLTPFIWNDLMQEQQNAQAYIDLEYAGVFSNIGAASNNEVERARQAASAGLSAAQKVK
CCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
ADQEITIQVIHSKLLEYRAHTEIAYSLYGHNPFFLMKDLSFKKIRDSLALPVPDVSVAYA
CCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECCCHHHHHHHHCCCCCCHHHHHH
AIDRAYRSAMELRRLSWVMAIIANQLPELAARRSQVEAATQTTRDAQQILSAERLSVVNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
ETNIRLHFLPGFLVERVAAAAGSTGGSLSQTLTNYKIAADNIRAVEQAAVRPYAIANPAI
ECCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHCCEEEHHHHHHHHHHHCCCEEECCCCC
NAPLSKPELEALKNLVDLQATIDLGKRWQDYHASLLHSESARHMAAAADAFAGLIARAQE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERLQEQIRIARELEARHLQEQARIAAEVEARRVAEEQARIAAEAEARRLAEEQARMVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIRNAHTFRAPSAASATGPLFMTSAGVVAVAEAAAASLQAAVRSAIAGLGSLAASVGAGA
HHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VVGVSALVYSSKLGNGELPDRYAFSTPLSDLAPDFAPDLHAIAAAGGTVDMPFRVSSKTD
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCEEECCCCC
AYGQSEVFVVKTDGRNIPSAVRVVAASYDTERNVYTATTFDVPPRTLTWTPIVNPGNSST
CCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCC
TSPAGQPDVPAYTGATVVPVEGRLDSFPGVFEAGFDDYVLVFPQDSGLPPNYTMFRDRRE
CCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCHHHHCCCC
DPGVASGVGQAVSGNWLGSASQGEGAPVPAQIADQLRGREFRNFRAFREAFWKAVADDSE
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHH
LVSQFNNSSAFEMKNGRAPFVRKIDRVGGQVKFELHHSVYISRGGEVMDVDNVRVVTPKQ
HHHHHCCCCCEEECCCCCHHHHHHHHCCCEEEEEEEEEEEEECCCCEEECCCEEEECCCC
HGAIHSEGD
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491711; 10984043 [H]