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The map label for this gene is rsmE [H]

Identifier: 77461509

GI number: 77461509

Start: 5951311

End: 5952030

Strand: Direct

Name: rsmE [H]

Synonym: Pfl01_5288

Alternate gene names: 77461509

Gene position: 5951311-5952030 (Clockwise)

Preceding gene: 77461508

Following gene: 77461512

Centisome position: 92.43

GC content: 65.0

Gene sequence:

>720_bases
ATGAGACTGTCCCGCTTCTTTATCGACGCCCCGCTCAGCACCGGCGAACACGAACTGCCGGAAGCCCAGGCGCATTACAT
CAGCCGCGTGCTGCGCATGGCCGAGGGCGATGCGGTGCAGTTGTTCGACGGTTCAGGCCACGAGTTTCGCGGCTCCCTGG
TGGAAGTCGGCAAGAAACGCGTGGTGGTGCAGATCGACGAGCAGTTCGCCGGTCAGGTCGAATCGCCGCTGAAGATCCAC
CTCGGCCAGGGCCTGTCCCGCGGCGAACGGATGGACTGGGCAATCCAGAAAGCCACCGAACTGGGCGTGACTGAAATCAC
CCCGATCTTCAGCGAGCGCTGCGAAGTCCGGCTCAAGGACGAACGCGCCGACAAACGCCTGCTGCACTGGCGCCAAGTGG
CGATCAGCGCTTGCGAGCAATGCGGTCGCTCGCGAGTGCCAGTGATTCATCCACCGGTGCTGCTGGCCGACTGGCTGAAG
CAGACCGAAGCCGAACTGAAACTGGTGCTGCACCCGGTGGCCGAGCCGCTGGTCAGCCATGCAAAACCATCGACCCTGGC
GTTCCTGATCGGCCCGGAAGGCGGTTTGTCCGACGCCGAAGTCGAGCAAGCCAAGGCCGGCGGCTTCCACGCCGCCCGCC
TCGGCCCGCGCGTGCTGCGCACCGAAACTGCGCCGGTTGTGGCGCTGGCGGTGGCGCAGCAACTGTGGGGTGATTTCTAA

Upstream 100 bases:

>100_bases
GCGTGGCGGTGCCGAAAGATTTCCATCCCGGGTTCCGCGATCCGGGCTAAGCATCACTCGCCACAACAGCGATCCTGATC
CAATTTTCTAGAGAACCACC

Downstream 100 bases:

>100_bases
CCCGTCACTGCACCGGATCACTGGTTGGTCTGGCGATGATTGCCTTGAGTTCGCGGGTCATCGGGAACTCAAGGTTCAGG
CCCTTCGGCGGGATCGGCTG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRLSRFFIDAPLSTGEHELPEAQAHYISRVLRMAEGDAVQLFDGSGHEFRGSLVEVGKKRVVVQIDEQFAGQVESPLKIH
LGQGLSRGERMDWAIQKATELGVTEITPIFSERCEVRLKDERADKRLLHWRQVAISACEQCGRSRVPVIHPPVLLADWLK
QTEAELKLVLHPVAEPLVSHAKPSTLAFLIGPEGGLSDAEVEQAKAGGFHAARLGPRVLRTETAPVVALAVAQQLWGDF

Sequences:

>Translated_239_residues
MRLSRFFIDAPLSTGEHELPEAQAHYISRVLRMAEGDAVQLFDGSGHEFRGSLVEVGKKRVVVQIDEQFAGQVESPLKIH
LGQGLSRGERMDWAIQKATELGVTEITPIFSERCEVRLKDERADKRLLHWRQVAISACEQCGRSRVPVIHPPVLLADWLK
QTEAELKLVLHPVAEPLVSHAKPSTLAFLIGPEGGLSDAEVEQAKAGGFHAARLGPRVLRTETAPVVALAVAQQLWGDF
>Mature_239_residues
MRLSRFFIDAPLSTGEHELPEAQAHYISRVLRMAEGDAVQLFDGSGHEFRGSLVEVGKKRVVVQIDEQFAGQVESPLKIH
LGQGLSRGERMDWAIQKATELGVTEITPIFSERCEVRLKDERADKRLLHWRQVAISACEQCGRSRVPVIHPPVLLADWLK
QTEAELKLVLHPVAEPLVSHAKPSTLAFLIGPEGGLSDAEVEQAKAGGFHAARLGPRVLRTETAPVVALAVAQQLWGDF

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=242, Percent_Identity=46.6942148760331, Blast_Score=206, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26371; Mature: 26371

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRFFIDAPLSTGEHELPEAQAHYISRVLRMAEGDAVQLFDGSGHEFRGSLVEVGKKR
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCE
VVVQIDEQFAGQVESPLKIHLGQGLSRGERMDWAIQKATELGVTEITPIFSERCEVRLKD
EEEEECCHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEECH
ERADKRLLHWRQVAISACEQCGRSRVPVIHPPVLLADWLKQTEAELKLVLHPVAEPLVSH
HHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AKPSTLAFLIGPEGGLSDAEVEQAKAGGFHAARLGPRVLRTETAPVVALAVAQQLWGDF
CCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLSRFFIDAPLSTGEHELPEAQAHYISRVLRMAEGDAVQLFDGSGHEFRGSLVEVGKKR
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCE
VVVQIDEQFAGQVESPLKIHLGQGLSRGERMDWAIQKATELGVTEITPIFSERCEVRLKD
EEEEECCHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEECH
ERADKRLLHWRQVAISACEQCGRSRVPVIHPPVLLADWLKQTEAELKLVLHPVAEPLVSH
HHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AKPSTLAFLIGPEGGLSDAEVEQAKAGGFHAARLGPRVLRTETAPVVALAVAQQLWGDF
CCCCEEEEEECCCCCCCHHHHHHHHCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]