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The map label for this gene is bioD [H]

Identifier: 77461393

GI number: 77461393

Start: 5817823

End: 5818503

Strand: Reverse

Name: bioD [H]

Synonym: Pfl01_5172

Alternate gene names: 77461393

Gene position: 5818503-5817823 (Counterclockwise)

Preceding gene: 77461394

Following gene: 77461392

Centisome position: 90.37

GC content: 64.9

Gene sequence:

>681_bases
ATGAGCGCAGCCTATTTCATCACCGGAACCGACACTGACGTTGGCAAGACCACCGTCGCCGCCGGGTTGCTGCACGCTGC
GCGGTCGGCGGGGCTGAGTACGGCGGCGGGCAAACCGGTAGCGTCCGGTTGCGTGGTGACGCCCAGAGGTCTGCGCAATT
CCGATGCGCTGGCGTTGCAGGCCGAATGCTCGTTGCCGCTGACGTATCAACAGGTCAATCCGGTGGCGTTCGAGCCAGCG
ATCGCGCCGCATCTGGCAGCGCGTGAGGCGGGTGTGGCGCTGACCGTGCAATCGTTGCTGGGGCCGATGCGCGAGATTCT
GGCCATGAACGCCGACTTCACGCTGATCGAAGGCGCAGGCGGCTGGCGGGTGCCGTTGGCGGATCAGGACAATCTGTCGG
ATCTGGCGATTGCGCTGAAGTTGCCGGTGATTCTGGTGGTCGGCGTGCGTCTGGGTTGCATCAGCCACGCGCTGTTGACC
GCTGAAGCGATCGCTCGTGACGGGCTGCAACTGGCCGGTTGGGTGGCGAACATCATTGATCCGAAGACGTCGCGGCTGGA
GGAAAACCTCGCGACACTCGCCGAGCGGATTCCGGCGCCGTGCCTGGGGCGGGTGCCGAAGCTGAAGGCGGTTAGCGCTG
AAGCGGTCGCCGATCACTTGCAACTGGATCTGCTCGACTGA

Upstream 100 bases:

>100_bases
CGCGGATTCTGGGGCTGGTCGAGGCCTACGAGCAGTTCCGTCAGCCTGCGGGATTGCCGGCGACCTACCAGGTGGTCTAC
GCCGTGTTGGAGAAACCCTT

Downstream 100 bases:

>100_bases
TTTTGGCTATGACAAGGCACTGTGCCATTAGTGTTTTTGACGGGTGTTTTTCTGACGCCTCTGGTTCAATGGCACCCATC
GATCCTTCCCAAGGATGAGA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSAAYFITGTDTDVGKTTVAAGLLHAARSAGLSTAAGKPVASGCVVTPRGLRNSDALALQAECSLPLTYQQVNPVAFEPA
IAPHLAAREAGVALTVQSLLGPMREILAMNADFTLIEGAGGWRVPLADQDNLSDLAIALKLPVILVVGVRLGCISHALLT
AEAIARDGLQLAGWVANIIDPKTSRLEENLATLAERIPAPCLGRVPKLKAVSAEAVADHLQLDLLD

Sequences:

>Translated_226_residues
MSAAYFITGTDTDVGKTTVAAGLLHAARSAGLSTAAGKPVASGCVVTPRGLRNSDALALQAECSLPLTYQQVNPVAFEPA
IAPHLAAREAGVALTVQSLLGPMREILAMNADFTLIEGAGGWRVPLADQDNLSDLAIALKLPVILVVGVRLGCISHALLT
AEAIARDGLQLAGWVANIIDPKTSRLEENLATLAERIPAPCLGRVPKLKAVSAEAVADHLQLDLLD
>Mature_225_residues
SAAYFITGTDTDVGKTTVAAGLLHAARSAGLSTAAGKPVASGCVVTPRGLRNSDALALQAECSLPLTYQQVNPVAFEPAI
APHLAAREAGVALTVQSLLGPMREILAMNADFTLIEGAGGWRVPLADQDNLSDLAIALKLPVILVVGVRLGCISHALLTA
EAIARDGLQLAGWVANIIDPKTSRLEENLATLAERIPAPCLGRVPKLKAVSAEAVADHLQLDLLD

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=208, Percent_Identity=51.4423076923077, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI87081944, Length=228, Percent_Identity=42.1052631578947, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 23407; Mature: 23276

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAYFITGTDTDVGKTTVAAGLLHAARSAGLSTAAGKPVASGCVVTPRGLRNSDALALQ
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCEEEE
AECSLPLTYQQVNPVAFEPAIAPHLAAREAGVALTVQSLLGPMREILAMNADFTLIEGAG
ECCCCCEEHHHCCCEEECCCHHHHHHHHHCCHHEEHHHHHHHHHHHHHCCCCEEEEECCC
GWRVPLADQDNLSDLAIALKLPVILVVGVRLGCISHALLTAEAIARDGLQLAGWVANIID
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
PKTSRLEENLATLAERIPAPCLGRVPKLKAVSAEAVADHLQLDLLD
CCHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHEEECC
>Mature Secondary Structure 
SAAYFITGTDTDVGKTTVAAGLLHAARSAGLSTAAGKPVASGCVVTPRGLRNSDALALQ
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCEEEE
AECSLPLTYQQVNPVAFEPAIAPHLAAREAGVALTVQSLLGPMREILAMNADFTLIEGAG
ECCCCCEEHHHCCCEEECCCHHHHHHHHHCCHHEEHHHHHHHHHHHHHCCCCEEEEECCC
GWRVPLADQDNLSDLAIALKLPVILVVGVRLGCISHALLTAEAIARDGLQLAGWVANIID
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
PKTSRLEENLATLAERIPAPCLGRVPKLKAVSAEAVADHLQLDLLD
CCHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA