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The map label for this gene is rpoD [H]

Identifier: 77461370

GI number: 77461370

Start: 5785829

End: 5787676

Strand: Direct

Name: rpoD [H]

Synonym: Pfl01_5149

Alternate gene names: 77461370

Gene position: 5785829-5787676 (Clockwise)

Preceding gene: 77461369

Following gene: 77461371

Centisome position: 89.86

GC content: 58.77

Gene sequence:

>1848_bases
ATGTCCGGAAAAGCGCAACAGCAGTCTCGTATTATTGAGTTGATCAAACTGGGTCGTGAGCAGAAGTATCTGACTTACGC
CGAGGTCAACGACCACCTGCCCGAGGATATTTCAGATCCGGAGCAGGTGGAAGACATCATCCGCATGATTAACGACATGG
GGATCCCCGTACACGAGAGTGCTCCGGATGCGGACGCCCTTATGCTGGCCGACGCCGATACCGACGAGGCCGCTGCGGAA
GAAGCAGCCGCTGCGTTGGCGGCGGTGGAGACCGATATCGGTCGCACCACTGACCCGGTGCGCATGTACATGCGTGAAAT
GGGTACGGTCGAGCTTCTGACTCGTGAAGGCGAAATCGAAATCGCCAAGCGTATCGAAGAAGGCATCCGTGAAGTGATGA
GCGCCATCGCGCACTTCCCTGGCACGGTTGACCATATTCTCTCCGAGTACACTCGCGTCACCACCGAAGGTGGTCGCCTG
TCCGACGTTCTGAGCGGTTACATCGACCCGGACGACGGCATTACGCCGCCTGCCGCCGAAGTACCGCCACCGATCGACGC
GAAAGCCGCGAAAGCGGATGACGACTCCGAGGACGATGACGCCGAAGCTTCCGATGACGAAGAAGAAGCCGAAAGCGGTC
CGGATCCGGTCATTGCCGCACAGCGCTTCGGTGCTGTCGCCGACCAGATGGAAATCACCCGCAAGGCCCTGAAAAAGCAC
GGTCGTCACAACAAGGCGGCAATTGCCGAACTGTTGGCCCTGGCCGAGCTGTTCATGCCGATCAAGCTGGTGCCGAAGCA
GTTCGAAGCCCTGGTCGAGCGTGTTCGCAGCGCCCTGGATCGCCTGCGTCAGCAAGAGCGCGCGATCATGCAACTGTGCG
TACGTGATGCACGTATGCCTCGTGCCGACTTCCTGCGCCAGTTCCCGGGCAACGAAGTCGACGAAAGCTGGTCCGACGCC
CTGGCCAAAGGCAAGAGCAAGTACGCCGAAGCCATCGCCCGCGTGCAACCGGACATCATCCGTTGCCAGCAAAAGCTGAC
CGCGCTGGAAACCGAGACCGGTTTGACCATCGCCGAGATCAAGGACATCAACCGTCGCATGTCGATCGGTGAGGCGAAAG
CCCGCCGCGCGAAGAAAGAGATGGTTGAAGCGAACTTGCGTCTGGTGATCTCCATCGCCAAGAAGTACACCAACCGTGGC
CTGCAATTCCTCGACCTGATCCAGGAAGGCAACATCGGTCTGATGAAAGCGGTGGACAAGTTCGAATACCGTCGTGGTTA
CAAGTTCTCGACTTACGCCACCTGGTGGATCCGTCAGGCGATCACTCGCTCGATCGCCGACCAGGCCCGCACCATCCGTA
TTCCGGTGCACATGATCGAGACGATCAACAAGCTCAACCGTATTTCCCGGCAGATGCTGCAGGAAATGGGTCGCGAACCG
ACCCCGGAAGAGCTGGGTGAACGCATGGAAATGCCTGAGGACAAGATCCGCAAGGTATTGAAGATCGCCAAAGAGCCGAT
CTCCATGGAAACCCCGATCGGTGATGACGAAGACTCTCATCTGGGTGACTTCATCGAAGACTCGACCATGCAGTCGCCAA
TCGATGTCGCCACTGTCGAGAGCCTGAAAGAAGCGACCCGCGAAGTGCTGTCCGGCCTTACTGCCCGTGAAGCCAAGGTA
CTGCGCATGCGTTTCGGTATCGACATGAACACCGACCATACGCTTGAAGAAGTCGGCAAACAGTTTGACGTGACCCGCGA
GCGGATCCGTCAGATCGAAGCCAAGGCGCTGCGCAAGCTGCGCCACCCGACGCGAAGCGAGCATCTGCGCTCCTTCCTCG
ACGAGTGA

Upstream 100 bases:

>100_bases
TCCGCATCAAACCCGACCTCAACTGGCGCGTGAGGTCATAGCTCAGGTATAATCCTCGGCTTGTTTTTTGCCCGCCAAGA
CCTTCAGTGGATAGGGTGTT

Downstream 100 bases:

>100_bases
TACCAGAACCCCCGGCCCAGGCCGGGGGTTTTGTTTATATGGCAGATTAAATACTCAGCTCCGCCCTCCCCCGCGCACAG
CCCGTCTACACTCGAAACAT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MSGKAQQQSRIIELIKLGREQKYLTYAEVNDHLPEDISDPEQVEDIIRMINDMGIPVHESAPDADALMLADADTDEAAAE
EAAAALAAVETDIGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEEGIREVMSAIAHFPGTVDHILSEYTRVTTEGGRL
SDVLSGYIDPDDGITPPAAEVPPPIDAKAAKADDDSEDDDAEASDDEEEAESGPDPVIAAQRFGAVADQMEITRKALKKH
GRHNKAAIAELLALAELFMPIKLVPKQFEALVERVRSALDRLRQQERAIMQLCVRDARMPRADFLRQFPGNEVDESWSDA
LAKGKSKYAEAIARVQPDIIRCQQKLTALETETGLTIAEIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRG
LQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREP
TPEELGERMEMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDSTMQSPIDVATVESLKEATREVLSGLTAREAKV
LRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTRSEHLRSFLDE

Sequences:

>Translated_615_residues
MSGKAQQQSRIIELIKLGREQKYLTYAEVNDHLPEDISDPEQVEDIIRMINDMGIPVHESAPDADALMLADADTDEAAAE
EAAAALAAVETDIGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEEGIREVMSAIAHFPGTVDHILSEYTRVTTEGGRL
SDVLSGYIDPDDGITPPAAEVPPPIDAKAAKADDDSEDDDAEASDDEEEAESGPDPVIAAQRFGAVADQMEITRKALKKH
GRHNKAAIAELLALAELFMPIKLVPKQFEALVERVRSALDRLRQQERAIMQLCVRDARMPRADFLRQFPGNEVDESWSDA
LAKGKSKYAEAIARVQPDIIRCQQKLTALETETGLTIAEIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRG
LQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREP
TPEELGERMEMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDSTMQSPIDVATVESLKEATREVLSGLTAREAKV
LRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTRSEHLRSFLDE
>Mature_614_residues
SGKAQQQSRIIELIKLGREQKYLTYAEVNDHLPEDISDPEQVEDIIRMINDMGIPVHESAPDADALMLADADTDEAAAEE
AAAALAAVETDIGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEEGIREVMSAIAHFPGTVDHILSEYTRVTTEGGRLS
DVLSGYIDPDDGITPPAAEVPPPIDAKAAKADDDSEDDDAEASDDEEEAESGPDPVIAAQRFGAVADQMEITRKALKKHG
RHNKAAIAELLALAELFMPIKLVPKQFEALVERVRSALDRLRQQERAIMQLCVRDARMPRADFLRQFPGNEVDESWSDAL
AKGKSKYAEAIARVQPDIIRCQQKLTALETETGLTIAEIKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGL
QFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPT
PEELGERMEMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDSTMQSPIDVATVESLKEATREVLSGLTAREAKVL
RMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPTRSEHLRSFLDE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=610, Percent_Identity=66.5573770491803, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=245, Percent_Identity=41.6326530612245, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=27.6859504132231, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=26.7326732673267, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 69305; Mature: 69174

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKAQQQSRIIELIKLGREQKYLTYAEVNDHLPEDISDPEQVEDIIRMINDMGIPVHES
CCCCHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APDADALMLADADTDEAAAEEAAAALAAVETDIGRTTDPVRMYMREMGTVELLTREGEIE
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCHHH
IAKRIEEGIREVMSAIAHFPGTVDHILSEYTRVTTEGGRLSDVLSGYIDPDDGITPPAAE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCC
VPPPIDAKAAKADDDSEDDDAEASDDEEEAESGPDPVIAAQRFGAVADQMEITRKALKKH
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GRHNKAAIAELLALAELFMPIKLVPKQFEALVERVRSALDRLRQQERAIMQLCVRDARMP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RADFLRQFPGNEVDESWSDALAKGKSKYAEAIARVQPDIIRCQQKLTALETETGLTIAEI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHH
KDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHH
FEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPEELGERMEMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDSTMQSPIDVATVE
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
SLKEATREVLSGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKL
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHPTRSEHLRSFLDE
HCCCHHHHHHHHHCC
>Mature Secondary Structure 
SGKAQQQSRIIELIKLGREQKYLTYAEVNDHLPEDISDPEQVEDIIRMINDMGIPVHES
CCCHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APDADALMLADADTDEAAAEEAAAALAAVETDIGRTTDPVRMYMREMGTVELLTREGEIE
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCHHH
IAKRIEEGIREVMSAIAHFPGTVDHILSEYTRVTTEGGRLSDVLSGYIDPDDGITPPAAE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCC
VPPPIDAKAAKADDDSEDDDAEASDDEEEAESGPDPVIAAQRFGAVADQMEITRKALKKH
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GRHNKAAIAELLALAELFMPIKLVPKQFEALVERVRSALDRLRQQERAIMQLCVRDARMP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RADFLRQFPGNEVDESWSDALAKGKSKYAEAIARVQPDIIRCQQKLTALETETGLTIAEI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHH
KDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHH
FEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPEELGERMEMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDSTMQSPIDVATVE
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
SLKEATREVLSGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKL
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHPTRSEHLRSFLDE
HCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665535 [H]