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The map label for this gene is dnaG [H]

Identifier: 77461369

GI number: 77461369

Start: 5783794

End: 5785761

Strand: Direct

Name: dnaG [H]

Synonym: Pfl01_5148

Alternate gene names: 77461369

Gene position: 5783794-5785761 (Clockwise)

Preceding gene: 77461368

Following gene: 77461370

Centisome position: 89.83

GC content: 61.28

Gene sequence:

>1968_bases
ATGGCCGGGCTGATTCCCCAGAGCTTCATTGACGACCTTCTGAACCGCACCGACATCGTCGACGTGGTCAGCTCGCGCGT
GCAATTGAAGAAGGCCGGCAAAAACTACACCGCCTGCTGCCCGTTCCATAAAGAGAAAACCCCGTCGTTCAGCGTCAGCC
CCGACAAACAGTTCTATTACTGTTTCGGCTGCGGCGCCGGCGGCAACGCCCTCGGCTTTCTCATGGATCACGACAACCTG
GACTTCCCCCAGGCTGTCGAAGACCTGGCCAAAGCCGCCGGCATGGAAGTGCCGCGTGATGAAGGCGGTCGTTCGAACAA
ACCGCGCCAGCCGACCGACTCACCGCTGTACCCGCTGCTCACCGCCGCCGCCGACTTTTATCGACAGGCGCTCAAAAGCC
ATCCGGCACGCAAGGCAGCGGTGGATTACCTCAAGGGTCGCGGCCTGACCGGCGAGATCGCCCGCGACTTCGGTCTCGGA
TTCGCACCACCGGGCTGGGACAACCTGCTCAAACACCTGAGCAGCGACACCTTGCAGCAGAAAGCCATGATCGAGGCCGG
CCTGCTGATCGAAAACGCCGAAACCGGCAAACGCTACGATCGCTTTCGCGATCGCGTGATGTTCCCGATCCGCGACACCC
GTGGACGGATCATCGCTTTCGGCGGTCGAGTGCTCGGCGACGACAAGCCAAAGTACCTGAACTCGCCGGAAACCCCGGTT
TTCCATAAAGGCCAGGAACTCTACGGCCTGTACGAAGCACGCAAGAACAATCGCAACCTCGACGAAATCATCGTCGTCGA
GGGCTATATGGATGTCATCGCCCTGGCCCAGCAAGGCCTGCGCAATGCCGTCGCGACACTGGGCACCGCCACCAGCGAAG
AACACCTCAAGCGCCTGTTTCGCGTCGTGCCGAACGTGCTGTTCTGCTTCGACGGCGATCAGGCCGGCCGCAATGCTGCC
TGGCGCGCACTGGAAGCAACACTGCCCAGCCTGCAAGACGGACGCCGTGCACGCTTTCTGTTTCTGCCCGAAGGCGAAGA
CCCGGACACATTGATTCGCGCCGAGGGCACTGACGCCTTCCGCGCCCGGATCAATCAGCACGCGCAGCCGCTGGCGGACT
ACTTCTTCCAGCAACTGACCGAAGAAGCCGACCCGCGCTCGCTGGAGGGCAAGGCCCACATGGCCACGCTCGCAGCACCG
TTGATCGACAAGGTCCCCGGCGCCAACCTGCGCATCCTGATGCGCCAGCGCCTGACGGAAATCACCGGCCTGAGCAGCGA
GAGCGTCAGCCAGCTGGCCCAGAGCGCACCGCAGGAAGCGCCGCCGGCCTACGACCCAGGCATCGATTACGACGCCATGC
CGGACTACAGCGACTACCATCAGTCGCAGGCACAGGACATGTATGTGCCGCAGCAGGAATGGACGCCGAAGAAATCCGGT
GCCGGCGGCAAGAAATGGGACAAGAAACCGTGGGACAAAAATGGCAAGCGTGGCGGTGATCGCGATCAGCCCCGTGCCCC
ACGCGTGCCGGCCGCCGTCGAACCACCGACCCTGGCCGCCCTGAGAACTTTGCTGCATCACCCGCAACTGGCGGAAAAAG
TCGAGGACGCCGGGCACTTCGCGGACGAGAACCAGACCAACGCACAGCTGCTGGTGGCACTGCTCGAAGCCGTACAGAAG
AATCCCAAGCTAAACTCATTTCAGTTGATCGCGCGATGGCACGGAACCGAACAGGGTCGCCTGCTCAAGGCGCTGGCGGA
AAAGGAATGGCTGATTGACGGAGACAACCTTGAACAACAGTTTTTCGACACCATTACTAGCTTGTCAGCCCGCCAACGCG
AGCGAAATCTGGAACAACTGCTCAGGAAAGCGCGTCAAAGCGAACTGAGCGCCGAAGAGAAAAATCAACTGCGCGACCTT
TTAAGTCGCAATGTTTCCGCATCAAACCCGACCTCAACTGGCGCGTGA

Upstream 100 bases:

>100_bases
AAACGACACCGGTCGAGCCGCCCTACGTGCGTGCATCACTCAATGACCCGCGTCCGGCCGCCTTTCGCATCGGATCCATT
ACAGCGCAGACGAGAACGCC

Downstream 100 bases:

>100_bases
GGTCATAGCTCAGGTATAATCCTCGGCTTGTTTTTTGCCCGCCAAGACCTTCAGTGGATAGGGTGTTATGTCCGGAAAAG
CGCAACAGCAGTCTCGTATT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MAGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYYCFGCGAGGNALGFLMDHDNL
DFPQAVEDLAKAAGMEVPRDEGGRSNKPRQPTDSPLYPLLTAAADFYRQALKSHPARKAAVDYLKGRGLTGEIARDFGLG
FAPPGWDNLLKHLSSDTLQQKAMIEAGLLIENAETGKRYDRFRDRVMFPIRDTRGRIIAFGGRVLGDDKPKYLNSPETPV
FHKGQELYGLYEARKNNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLFRVVPNVLFCFDGDQAGRNAA
WRALEATLPSLQDGRRARFLFLPEGEDPDTLIRAEGTDAFRARINQHAQPLADYFFQQLTEEADPRSLEGKAHMATLAAP
LIDKVPGANLRILMRQRLTEITGLSSESVSQLAQSAPQEAPPAYDPGIDYDAMPDYSDYHQSQAQDMYVPQQEWTPKKSG
AGGKKWDKKPWDKNGKRGGDRDQPRAPRVPAAVEPPTLAALRTLLHHPQLAEKVEDAGHFADENQTNAQLLVALLEAVQK
NPKLNSFQLIARWHGTEQGRLLKALAEKEWLIDGDNLEQQFFDTITSLSARQRERNLEQLLRKARQSELSAEEKNQLRDL
LSRNVSASNPTSTGA

Sequences:

>Translated_655_residues
MAGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYYCFGCGAGGNALGFLMDHDNL
DFPQAVEDLAKAAGMEVPRDEGGRSNKPRQPTDSPLYPLLTAAADFYRQALKSHPARKAAVDYLKGRGLTGEIARDFGLG
FAPPGWDNLLKHLSSDTLQQKAMIEAGLLIENAETGKRYDRFRDRVMFPIRDTRGRIIAFGGRVLGDDKPKYLNSPETPV
FHKGQELYGLYEARKNNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLFRVVPNVLFCFDGDQAGRNAA
WRALEATLPSLQDGRRARFLFLPEGEDPDTLIRAEGTDAFRARINQHAQPLADYFFQQLTEEADPRSLEGKAHMATLAAP
LIDKVPGANLRILMRQRLTEITGLSSESVSQLAQSAPQEAPPAYDPGIDYDAMPDYSDYHQSQAQDMYVPQQEWTPKKSG
AGGKKWDKKPWDKNGKRGGDRDQPRAPRVPAAVEPPTLAALRTLLHHPQLAEKVEDAGHFADENQTNAQLLVALLEAVQK
NPKLNSFQLIARWHGTEQGRLLKALAEKEWLIDGDNLEQQFFDTITSLSARQRERNLEQLLRKARQSELSAEEKNQLRDL
LSRNVSASNPTSTGA
>Mature_654_residues
AGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYYCFGCGAGGNALGFLMDHDNLD
FPQAVEDLAKAAGMEVPRDEGGRSNKPRQPTDSPLYPLLTAAADFYRQALKSHPARKAAVDYLKGRGLTGEIARDFGLGF
APPGWDNLLKHLSSDTLQQKAMIEAGLLIENAETGKRYDRFRDRVMFPIRDTRGRIIAFGGRVLGDDKPKYLNSPETPVF
HKGQELYGLYEARKNNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLFRVVPNVLFCFDGDQAGRNAAW
RALEATLPSLQDGRRARFLFLPEGEDPDTLIRAEGTDAFRARINQHAQPLADYFFQQLTEEADPRSLEGKAHMATLAAPL
IDKVPGANLRILMRQRLTEITGLSSESVSQLAQSAPQEAPPAYDPGIDYDAMPDYSDYHQSQAQDMYVPQQEWTPKKSGA
GGKKWDKKPWDKNGKRGGDRDQPRAPRVPAAVEPPTLAALRTLLHHPQLAEKVEDAGHFADENQTNAQLLVALLEAVQKN
PKLNSFQLIARWHGTEQGRLLKALAEKEWLIDGDNLEQQFFDTITSLSARQRERNLEQLLRKARQSELSAEEKNQLRDLL
SRNVSASNPTSTGA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=440, Percent_Identity=53.8636363636364, Blast_Score=500, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 72926; Mature: 72795

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNALGFLMDHDNLDFPQAVEDLAKAAGMEVPRDEGGRSNKPRQPTDSPLYPLL
EEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
TAAADFYRQALKSHPARKAAVDYLKGRGLTGEIARDFGLGFAPPGWDNLLKHLSSDTLQQ
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KAMIEAGLLIENAETGKRYDRFRDRVMFPIRDTRGRIIAFGGRVLGDDKPKYLNSPETPV
HHHHHHHHEEECCCCHHHHHHHHHHEECCEECCCCCEEEECCEEECCCCCCCCCCCCCCC
FHKGQELYGLYEARKNNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLF
CCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RVVPNVLFCFDGDQAGRNAAWRALEATLPSLQDGRRARFLFLPEGEDPDTLIRAEGTDAF
HHHCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHH
RARINQHAQPLADYFFQQLTEEADPRSLEGKAHMATLAAPLIDKVPGANLRILMRQRLTE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ITGLSSESVSQLAQSAPQEAPPAYDPGIDYDAMPDYSDYHQSQAQDMYVPQQEWTPKKSG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCC
AGGKKWDKKPWDKNGKRGGDRDQPRAPRVPAAVEPPTLAALRTLLHHPQLAEKVEDAGHF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCC
ADENQTNAQLLVALLEAVQKNPKLNSFQLIARWHGTEQGRLLKALAEKEWLIDGDNLEQQ
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHH
FFDTITSLSARQRERNLEQLLRKARQSELSAEEKNQLRDLLSRNVSASNPTSTGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AGLIPQSFIDDLLNRTDIVDVVSSRVQLKKAGKNYTACCPFHKEKTPSFSVSPDKQFYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEE
CFGCGAGGNALGFLMDHDNLDFPQAVEDLAKAAGMEVPRDEGGRSNKPRQPTDSPLYPLL
EEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
TAAADFYRQALKSHPARKAAVDYLKGRGLTGEIARDFGLGFAPPGWDNLLKHLSSDTLQQ
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
KAMIEAGLLIENAETGKRYDRFRDRVMFPIRDTRGRIIAFGGRVLGDDKPKYLNSPETPV
HHHHHHHHEEECCCCHHHHHHHHHHEECCEECCCCCEEEECCEEECCCCCCCCCCCCCCC
FHKGQELYGLYEARKNNRNLDEIIVVEGYMDVIALAQQGLRNAVATLGTATSEEHLKRLF
CCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RVVPNVLFCFDGDQAGRNAAWRALEATLPSLQDGRRARFLFLPEGEDPDTLIRAEGTDAF
HHHCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHH
RARINQHAQPLADYFFQQLTEEADPRSLEGKAHMATLAAPLIDKVPGANLRILMRQRLTE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ITGLSSESVSQLAQSAPQEAPPAYDPGIDYDAMPDYSDYHQSQAQDMYVPQQEWTPKKSG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCC
AGGKKWDKKPWDKNGKRGGDRDQPRAPRVPAAVEPPTLAALRTLLHHPQLAEKVEDAGHF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCC
ADENQTNAQLLVALLEAVQKNPKLNSFQLIARWHGTEQGRLLKALAEKEWLIDGDNLEQQ
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHH
FFDTITSLSARQRERNLEQLLRKARQSELSAEEKNQLRDLLSRNVSASNPTSTGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]