Click here to switch to the map view.

The map label for this gene is vfr [H]

Identifier: 77461332

GI number: 77461332

Start: 5744035

End: 5744679

Strand: Direct

Name: vfr [H]

Synonym: Pfl01_5111

Alternate gene names: 77461332

Gene position: 5744035-5744679 (Clockwise)

Preceding gene: 77461329

Following gene: 77461338

Centisome position: 89.22

GC content: 59.53

Gene sequence:

>645_bases
ATGGTTGGTATTACCCCCACACCCAAAATCAAAAACCTCGACAAGCTGCTGATGCATTGCCAGCGCCGGCGTCACGCGGC
CAAGAGCAACATCATCTGCGCCGGGGATCGCTCCGACACGCTTTACTTCATCATCCGCGGTTCGGTCACGATCCTGATCG
AGGACGACGACGGCCGCGAGATGATCATCGCGTACCTGAATGCCGGGGACTTCTTCGGCGAGCTGGGCCTTTTCGAAGAG
GCCGGCCAGGAACAGCAGCGCAGCGCCTGGGTGCGGGCCAAGGTTGAATGCGAAGTCGCGGAAATCAGCTACGCCAAGTT
CCGCGAACTGTCGCAGCAGGATCCAGACATTCTTTACGTGCTCAGCGGACAAATCGCACAACGCCTGCGCAACACCACGC
GCAAGGTTGGCGACCTGGCGTTCTTCGACGTCACCGGCCGTGTCGCTCGCTGCCTGCTGGAACTGTGCAAACAACCGGAC
GCCATGACTCACCCGGACGGCATGCAGATCAAAGTGACCCGTCAGGAAATCGGCCGGATTGTCGGTTGTTCGCGAGAGAT
GGTCGGTCGCGTGCTCAAGGATCTTGAGGAACGCAACCTGGTGGATGTGAAAGGCAAGACCATGGTGGTCTTCGGCACTC
GCTGA

Upstream 100 bases:

>100_bases
GGCGCCAATTCACTGGCGCCTTTGCCAGACTGTGTAGCGACCGCCTCAGCGCCTCATATAAAAACCAAATATAACCGTGC
AGTTTTTTCGGGATACAACC

Downstream 100 bases:

>100_bases
CTGCCAAAACTCAGCCGGCGTAGATCTGCGCGAGCATCAAACGGAAGAGTTCATCAAGACGCGCCAGCGCATGGGGCGCC
GGGAATTTTTCATGCAGAGC

Product: cAMP-regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MVGITPTPKIKNLDKLLMHCQRRRHAAKSNIICAGDRSDTLYFIIRGSVTILIEDDDGREMIIAYLNAGDFFGELGLFEE
AGQEQQRSAWVRAKVECEVAEISYAKFRELSQQDPDILYVLSGQIAQRLRNTTRKVGDLAFFDVTGRVARCLLELCKQPD
AMTHPDGMQIKVTRQEIGRIVGCSREMVGRVLKDLEERNLVDVKGKTMVVFGTR

Sequences:

>Translated_214_residues
MVGITPTPKIKNLDKLLMHCQRRRHAAKSNIICAGDRSDTLYFIIRGSVTILIEDDDGREMIIAYLNAGDFFGELGLFEE
AGQEQQRSAWVRAKVECEVAEISYAKFRELSQQDPDILYVLSGQIAQRLRNTTRKVGDLAFFDVTGRVARCLLELCKQPD
AMTHPDGMQIKVTRQEIGRIVGCSREMVGRVLKDLEERNLVDVKGKTMVVFGTR
>Mature_214_residues
MVGITPTPKIKNLDKLLMHCQRRRHAAKSNIICAGDRSDTLYFIIRGSVTILIEDDDGREMIIAYLNAGDFFGELGLFEE
AGQEQQRSAWVRAKVECEVAEISYAKFRELSQQDPDILYVLSGQIAQRLRNTTRKVGDLAFFDVTGRVARCLLELCKQPD
AMTHPDGMQIKVTRQEIGRIVGCSREMVGRVLKDLEERNLVDVKGKTMVVFGTR

Specific function: Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=203, Percent_Identity=63.5467980295566, Blast_Score=279, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24193; Mature: 24193

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00889 CNMP_BINDING_2 ; PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGITPTPKIKNLDKLLMHCQRRRHAAKSNIICAGDRSDTLYFIIRGSVTILIEDDDGRE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEECCEEEEEECCCCCE
MIIAYLNAGDFFGELGLFEEAGQEQQRSAWVRAKVECEVAEISYAKFRELSQQDPDILYV
EEEEEECCCHHHHHCCCHHHHCHHHHHHHHHEEEEEEEHHHHHHHHHHHHCCCCCCEEEE
LSGQIAQRLRNTTRKVGDLAFFDVTGRVARCLLELCKQPDAMTHPDGMQIKVTRQEIGRI
ECHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHH
VGCSREMVGRVLKDLEERNLVDVKGKTMVVFGTR
HCCCHHHHHHHHHHHHHCCEEEECCCEEEEEECC
>Mature Secondary Structure
MVGITPTPKIKNLDKLLMHCQRRRHAAKSNIICAGDRSDTLYFIIRGSVTILIEDDDGRE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEECCEEEEEECCCCCE
MIIAYLNAGDFFGELGLFEEAGQEQQRSAWVRAKVECEVAEISYAKFRELSQQDPDILYV
EEEEEECCCHHHHHCCCHHHHCHHHHHHHHHEEEEEEEHHHHHHHHHHHHCCCCCCEEEE
LSGQIAQRLRNTTRKVGDLAFFDVTGRVARCLLELCKQPDAMTHPDGMQIKVTRQEIGRI
ECHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHH
VGCSREMVGRVLKDLEERNLVDVKGKTMVVFGTR
HCCCHHHHHHHHHHHHHCCEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002577; 10984043 [H]