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The map label for this gene is tuf [H]

Identifier: 77461314

GI number: 77461314

Start: 5722362

End: 5723555

Strand: Reverse

Name: tuf [H]

Synonym: Pfl01_5093

Alternate gene names: 77461314

Gene position: 5723555-5722362 (Counterclockwise)

Preceding gene: 77461315

Following gene: 77461313

Centisome position: 88.9

GC content: 55.28

Gene sequence:

>1194_bases
ATGGCTAAGGAAAAATTTGATCGTTCCCTGCCGCACGTCAACGTTGGCACCATCGGTCACGTAGACCACGGTAAGACCAC
GCTGACCGCTGCTCTGACTCGCGTCTGCTCCGAAGTTTTCGGTTCGGCCGTAGTTGAATTCGACAAGATCGACAGCGCTC
CAGAAGAAAAAGCTCGCGGTATCACCATCAATACCGCGCACGTTGAGTACAACTCGAACATTCGTCACTACGCTCACGTT
GACTGCCCAGGTCACGCTGACTACGTGAAGAACATGATCACTGGTGCTGCGCAGATGGACGGCGCGATCCTGGTTTGCTC
GGCCGCTGATGGTCCGATGCCACAAACTCGTGAGCACATCCTGCTGTCCCGTCAGGTAGGCGTTCCGTACATCGTGGTTT
TCCTGAACAAGGCTGACCTGGTAGACGACGCTGAGCTGCTGGAACTGGTTGAGATGGAAGTTCGCGACCTGCTGTCCACC
TACGACTTCCCAGGCGACGACACTCCTATCATCATCGGTTCGGCTCGTATGGCGCTGGAAGGCAAAGACGACAACGAAAT
GGGCACCACCGCTGTTAAGAAGCTGGTGGAAACTCTGGACAGCTACATCCCAGAGCCAGAGCGTGCTATCGACAAGCCAT
TCCTGATGCCAATCGAAGACGTATTCTCGATCTCGGGTCGCGGTACTGTTGTAACCGGTCGTATCGAGCGCGGTATCGTT
CGCGTTCAAGACGCCCTGGAAATCGTTGGTCTGCGTGACACCACCACCACCACCTGCACCGGTGTTGAGATGTTCCGCAA
GCTGCTGGACGAAGGTCGTGCTGGCGAGAACTGCGGCGTTCTGCTGCGTGGTACCAAGCGTGACGACGTTGAGCGTGGTC
AGGTTCTGGTTAAGCCGGGTTCGGTTAAGCCGCACACCAAGTTCACCGCAGAAGTTTACGTTCTGAGCAAGGAAGAAGGC
GGTCGTCACACTCCGTTCTTCAAAGGCTATCGTCCACAGTTCTACTTCCGTACTACTGACGTGACCGGTAACTGCGAGCT
GCCAGAAGGCGTTGAAATGGTAATGCCAGGTGACAACATTCAGATGACTGTTACCCTGATCAAAACCATCGCTATGGAAG
ACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGTGCTGGCGTCGTAGCCAAGATCATCGAGTAA

Upstream 100 bases:

>100_bases
CGCTCAAGAGCTCCATATAACAAGGCAGATATGAAAATATCTGCCTTTGTTTTAATGGCTCGTGTCGTTTGCTCAATTTC
TTCTGTTAGGGGTGATATCG

Downstream 100 bases:

>100_bases
ACAGTTGTAATGTCTTTTTCAGGCCGGCATAATGGTCGGCCTGATTTTGTTTTAGGTCAGTAGCTCAATTGGCAGAGCGA
CGGTCTCCAAAACCGTAGGT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu-A [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAVVEFDKIDSAPEEKARGITINTAHVEYNSNIRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLST
YDFPGDDTPIIIGSARMALEGKDDNEMGTTAVKKLVETLDSYIPEPERAIDKPFLMPIEDVFSISGRGTVVTGRIERGIV
RVQDALEIVGLRDTTTTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPGSVKPHTKFTAEVYVLSKEEG
GRHTPFFKGYRPQFYFRTTDVTGNCELPEGVEMVMPGDNIQMTVTLIKTIAMEDGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_397_residues
MAKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAVVEFDKIDSAPEEKARGITINTAHVEYNSNIRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLST
YDFPGDDTPIIIGSARMALEGKDDNEMGTTAVKKLVETLDSYIPEPERAIDKPFLMPIEDVFSISGRGTVVTGRIERGIV
RVQDALEIVGLRDTTTTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPGSVKPHTKFTAEVYVLSKEEG
GRHTPFFKGYRPQFYFRTTDVTGNCELPEGVEMVMPGDNIQMTVTLIKTIAMEDGLRFAIREGGRTVGAGVVAKIIE
>Mature_396_residues
AKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAVVEFDKIDSAPEEKARGITINTAHVEYNSNIRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLSTY
DFPGDDTPIIIGSARMALEGKDDNEMGTTAVKKLVETLDSYIPEPERAIDKPFLMPIEDVFSISGRGTVVTGRIERGIVR
VQDALEIVGLRDTTTTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPGSVKPHTKFTAEVYVLSKEEGG
RHTPFFKGYRPQFYFRTTDVTGNCELPEGVEMVMPGDNIQMTVTLIKTIAMEDGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=54.6835443037975, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI4503475, Length=360, Percent_Identity=30.5555555555556, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4503471, Length=461, Percent_Identity=29.0672451193059, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI5729864, Length=433, Percent_Identity=27.9445727482679, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=27.9445727482679, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=27.7456647398844, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI194018522, Length=318, Percent_Identity=26.4150943396226, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI194097354, Length=318, Percent_Identity=26.4150943396226, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI194018520, Length=318, Percent_Identity=26.4150943396226, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI46094014, Length=331, Percent_Identity=27.4924471299094, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI4503507, Length=270, Percent_Identity=27.4074074074074, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI82546879, Length=440, Percent_Identity=24.0909090909091, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=78.2828282828283, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=78.2828282828283, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI2367247, Length=337, Percent_Identity=30.5637982195846, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI48994988, Length=250, Percent_Identity=32.4, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789108, Length=395, Percent_Identity=25.5696202531646, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788922, Length=327, Percent_Identity=25.0764525993884, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=50.75, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=45.8549222797927, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17552884, Length=459, Percent_Identity=28.7581699346405, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=459, Percent_Identity=28.7581699346405, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=443, Percent_Identity=28.4424379232506, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=27.0454545454545, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=440, Percent_Identity=27.0454545454545, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=246, Percent_Identity=30.8943089430894, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32566629, Length=336, Percent_Identity=26.4880952380952, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=35.0649350649351, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=26.241134751773, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=27.7310924369748, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=62.72040302267, Blast_Score=509, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6325337, Length=448, Percent_Identity=27.6785714285714, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=448, Percent_Identity=27.6785714285714, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=27.4052478134111, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6322937, Length=363, Percent_Identity=24.5179063360882, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=279, Percent_Identity=25.8064516129032, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=27.3858921161826, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=54.0609137055838, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=54.0609137055838, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=50.3875968992248, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=26.9736842105263, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=26.9736842105263, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=26.9736842105263, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=26.9736842105263, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24652838, Length=456, Percent_Identity=27.4122807017544, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI17137572, Length=456, Percent_Identity=27.4122807017544, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=30.718954248366, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI45550900, Length=343, Percent_Identity=27.4052478134111, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=28.2798833819242, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=25.6505576208178, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=25.6505576208178, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43499; Mature: 43368

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAVVEFDKIDSAPEEKARG
CCCHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHEEHHHCCCCCHHHHCC
ITINTAHVEYNSNIRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLSTYDFPGDDTPIIIGSARMALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEE
GKDDNEMGTTAVKKLVETLDSYIPEPERAIDKPFLMPIEDVFSISGRGTVVTGRIERGIV
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECHHHHCHH
RVQDALEIVGLRDTTTTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPG
HHHHHHHHEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEECC
SVKPHTKFTAEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGNCELPEGVEMVMPGDNI
CCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCC
QMTVTLIKTIAMEDGLRFAIREGGRTVGAGVVAKIIE
EEHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFDRSLPHVNVGTIGHVDHGKTTLTAALTRVCSEVFGSAVVEFDKIDSAPEEKARG
CCHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHEEHHHCCCCCHHHHCC
ITINTAHVEYNSNIRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPYIVVFLNKADLVDDAELLELVEMEVRDLLSTYDFPGDDTPIIIGSARMALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEE
GKDDNEMGTTAVKKLVETLDSYIPEPERAIDKPFLMPIEDVFSISGRGTVVTGRIERGIV
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECHHHHCHH
RVQDALEIVGLRDTTTTTCTGVEMFRKLLDEGRAGENCGVLLRGTKRDDVERGQVLVKPG
HHHHHHHHEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEEECC
SVKPHTKFTAEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGNCELPEGVEMVMPGDNI
CCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCC
QMTVTLIKTIAMEDGLRFAIREGGRTVGAGVVAKIIE
EEHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]