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The map label for this gene is btuB [H]

Identifier: 77461229

GI number: 77461229

Start: 5644984

End: 5646867

Strand: Direct

Name: btuB [H]

Synonym: Pfl01_5008

Alternate gene names: 77461229

Gene position: 5644984-5646867 (Clockwise)

Preceding gene: 77461228

Following gene: 77461242

Centisome position: 87.68

GC content: 60.35

Gene sequence:

>1884_bases
ATGAACCTCTCGCGCCTCGCCCTGCCCCTCCTGCTGCTGCCCTCCGCCAACGCCCTCGCTGACAATTTCGAACGCGACCA
GGCCCTGAAGCTGCCGGACGTGCTGATCAGCGCCAACCGTCAGGTCGAAGCGCGCAACGACAGCAGCGCCGCCAACACCG
TGTTCACCCGCGAAGACATCGAGCGTCTGCAACCGCGCAGCGTCACCGATCTGCTGCAACGGGTACCGGGCGTGCAAGTG
GCGCAAACCGGCGGACGTGGCAGCCTCCCGGGGATCTACATTCGCGGCACGCAATCGGCGCAGAGCCTGGTGCTGGTCGA
CGGCCAGCGCATCGGCAACTCCACTTCCGGCGACAGCAACCTGCAACACCTCAACATCGAGCAGATCGAGCGCGTGGAAG
TGCTGCGCGGTTCACGTTCGGTGATTTACGGTAGCGATGCAATTGGCGGGGTGATTCAGATCTTCACCCGGCGCGGCGGC
GAACAAGGCTTGCAGCCGCGGATGCATGTCGGATTCGGCAGCAACCAGACCTGGGAACGCAGTATTGGCCTCTCCGGCGG
AGACGAGAAAACCCGCTTCAACCTCGGCGCCAGCCTCGATGAAACCGCCGGCCTCGACCGCACCCATGAGTCCTACCCCA
GCGACAGCGATCACGATGCCTACCGCAACAAATCCATCAGCCTGAGCCTCAGCCATGCGCTGACCGATGACATCGAAGTC
GGCGCCAATCTGCTGGATAACAGGGGCAAAAGCGAGTTCGACAGTCCATGGGGACGTTACGTTGAAGCAACCGACCGATC
CTATGAAGAGCAACCTTACAGCGATTTCACCGTCAGCAGTTTCAGCAGCTACATCGATGCGCGGATCAACGAGCGCTGGA
GGTCGCGCCTGGAACTCGGCCACAGCGAAAATCGTGAAAAAACCTTCGACAAGCTCAGCGATGAACGCTCGGTGTTCAAC
ACCTACCGTGATTCGGTAACCTGGCAGAACGACCTGACCCTCGATGCGCGCAACAGCCTGATCCTTGGTGGCAACTGGTA
CGAAGACCGGATCAACAGCAGCACCGCGTTCGACGAAGACAGTCGCTGGAACCGCGCCGCATTTATCCAGCATCGTTATC
AGGCCGACAGCTTCTCCACCGAACTGGGCCTGCGTCGCGATGACAACCAGCAGTTCGGCGGTCAGAACAGCTGGAGCGGC
ACGCTGACGTTGCCGGTCAATCCCGACAACGATCTGCTCTTGAGCTACAGCGAAGGCTTCCGTGCCCCTACTTTCAACGA
CCTGTACTACCCCAAGTACAGCAATCCGGATCTGAAACCGGAAACCTCCAAAAGCTATGAGTTGCAGTGGCGCAGCCAGT
TGACTGACAGCAGCCGTCTAGAGGCGTCGATTTATCGTACAGATCTGAAGGACGCCATTATCGCCGCTGACGGTAACCCT
CCAGAGAACGTCGCCTCGGCGCGAATCAACGGCTTCGAAGCAACGCTAAAACAGGAGCTGTTCGGCTGGCAGAGCAACCT
CGGCGTGGCGATCATCGATCCCCGCGACCGCGACACCGGCCACACCTTGGCACGTCGTGCCCGTCGCACCTTGAGCTGGG
ATCTGGATCGACAGTTCGATCGCCTTGGCCTCGGCGCCAGTTGGCAGGCCGTGAGCAGCAGCTATGACGACCTGAACAAT
CAGCAGCCGCTTGGCGGTTATGCACTGCTCGGACTGCGCAGCAGCTGGGCGTTGAACCGCGAGATCAAGCTGGATTTCAA
GATCGACAACCTGCTGGACAAGGGTTACAGCCGGGCGCTGTACAGCCATGACGGCAGTCAGTATGACTATCGCGAGGAAG
GTCGGGCGTTCATGTTCGGCGTGACCTGGACACCGCAGCTCTAA

Upstream 100 bases:

>100_bases
GTCGGATCAGTCGATGATGAGTTGACTGACACTCCGCTTTCGCGAGCAAGCCCGCTCCCACATTTGATTTGTGTACACCT
GAAAGACCTACGGAATACCG

Downstream 100 bases:

>100_bases
CGATCTGGCGCGATCAGGTGGCAGAGTTTGGCGGTCGCCTCGATCATCTGCCCGCTCGGGCGCTCCAGACCTTTGTCGGT
GACCAGCAGTAATTGCCGAT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MNLSRLALPLLLLPSANALADNFERDQALKLPDVLISANRQVEARNDSSAANTVFTREDIERLQPRSVTDLLQRVPGVQV
AQTGGRGSLPGIYIRGTQSAQSLVLVDGQRIGNSTSGDSNLQHLNIEQIERVEVLRGSRSVIYGSDAIGGVIQIFTRRGG
EQGLQPRMHVGFGSNQTWERSIGLSGGDEKTRFNLGASLDETAGLDRTHESYPSDSDHDAYRNKSISLSLSHALTDDIEV
GANLLDNRGKSEFDSPWGRYVEATDRSYEEQPYSDFTVSSFSSYIDARINERWRSRLELGHSENREKTFDKLSDERSVFN
TYRDSVTWQNDLTLDARNSLILGGNWYEDRINSSTAFDEDSRWNRAAFIQHRYQADSFSTELGLRRDDNQQFGGQNSWSG
TLTLPVNPDNDLLLSYSEGFRAPTFNDLYYPKYSNPDLKPETSKSYELQWRSQLTDSSRLEASIYRTDLKDAIIAADGNP
PENVASARINGFEATLKQELFGWQSNLGVAIIDPRDRDTGHTLARRARRTLSWDLDRQFDRLGLGASWQAVSSSYDDLNN
QQPLGGYALLGLRSSWALNREIKLDFKIDNLLDKGYSRALYSHDGSQYDYREEGRAFMFGVTWTPQL

Sequences:

>Translated_627_residues
MNLSRLALPLLLLPSANALADNFERDQALKLPDVLISANRQVEARNDSSAANTVFTREDIERLQPRSVTDLLQRVPGVQV
AQTGGRGSLPGIYIRGTQSAQSLVLVDGQRIGNSTSGDSNLQHLNIEQIERVEVLRGSRSVIYGSDAIGGVIQIFTRRGG
EQGLQPRMHVGFGSNQTWERSIGLSGGDEKTRFNLGASLDETAGLDRTHESYPSDSDHDAYRNKSISLSLSHALTDDIEV
GANLLDNRGKSEFDSPWGRYVEATDRSYEEQPYSDFTVSSFSSYIDARINERWRSRLELGHSENREKTFDKLSDERSVFN
TYRDSVTWQNDLTLDARNSLILGGNWYEDRINSSTAFDEDSRWNRAAFIQHRYQADSFSTELGLRRDDNQQFGGQNSWSG
TLTLPVNPDNDLLLSYSEGFRAPTFNDLYYPKYSNPDLKPETSKSYELQWRSQLTDSSRLEASIYRTDLKDAIIAADGNP
PENVASARINGFEATLKQELFGWQSNLGVAIIDPRDRDTGHTLARRARRTLSWDLDRQFDRLGLGASWQAVSSSYDDLNN
QQPLGGYALLGLRSSWALNREIKLDFKIDNLLDKGYSRALYSHDGSQYDYREEGRAFMFGVTWTPQL
>Mature_627_residues
MNLSRLALPLLLLPSANALADNFERDQALKLPDVLISANRQVEARNDSSAANTVFTREDIERLQPRSVTDLLQRVPGVQV
AQTGGRGSLPGIYIRGTQSAQSLVLVDGQRIGNSTSGDSNLQHLNIEQIERVEVLRGSRSVIYGSDAIGGVIQIFTRRGG
EQGLQPRMHVGFGSNQTWERSIGLSGGDEKTRFNLGASLDETAGLDRTHESYPSDSDHDAYRNKSISLSLSHALTDDIEV
GANLLDNRGKSEFDSPWGRYVEATDRSYEEQPYSDFTVSSFSSYIDARINERWRSRLELGHSENREKTFDKLSDERSVFN
TYRDSVTWQNDLTLDARNSLILGGNWYEDRINSSTAFDEDSRWNRAAFIQHRYQADSFSTELGLRRDDNQQFGGQNSWSG
TLTLPVNPDNDLLLSYSEGFRAPTFNDLYYPKYSNPDLKPETSKSYELQWRSQLTDSSRLEASIYRTDLKDAIIAADGNP
PENVASARINGFEATLKQELFGWQSNLGVAIIDPRDRDTGHTLARRARRTLSWDLDRQFDRLGLGASWQAVSSSYDDLNN
QQPLGGYALLGLRSSWALNREIKLDFKIDNLLDKGYSRALYSHDGSQYDYREEGRAFMFGVTWTPQL

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=625, Percent_Identity=29.44, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1788478, Length=133, Percent_Identity=36.0902255639098, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786798, Length=737, Percent_Identity=22.3880597014925, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 70632; Mature: 70632

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSRLALPLLLLPSANALADNFERDQALKLPDVLISANRQVEARNDSSAANTVFTREDI
CCHHHHHHHEEEECCCHHHHHCCCCCCCCCCCHHHHCCCCEEECCCCCCHHHEEEEHHHH
ERLQPRSVTDLLQRVPGVQVAQTGGRGSLPGIYIRGTQSAQSLVLVDGQRIGNSTSGDSN
HHCCCCCHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEECHHCCCCCCCCCC
LQHLNIEQIERVEVLRGSRSVIYGSDAIGGVIQIFTRRGGEQGLQPRMHVGFGSNQTWER
CEECCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHH
SIGLSGGDEKTRFNLGASLDETAGLDRTHESYPSDSDHDAYRNKSISLSLSHALTDDIEV
HCCCCCCCCCEEEECCCCCHHHCCCCCCHHCCCCCCCCHHHCCCEEEEEEHHHHCCHHHH
GANLLDNRGKSEFDSPWGRYVEATDRSYEEQPYSDFTVSSFSSYIDARINERWRSRLELG
HHHHHHCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
HSENREKTFDKLSDERSVFNTYRDSVTWQNDLTLDARNSLILGGNWYEDRINSSTAFDED
CCCCHHHHHHHHHHHHHHHHHHHHCCEECCCEEEECCCCEEECCCCHHHHCCCCCCCCCC
SRWNRAAFIQHRYQADSFSTELGLRRDDNQQFGGQNSWSGTLTLPVNPDNDLLLSYSEGF
CCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCC
RAPTFNDLYYPKYSNPDLKPETSKSYELQWRSQLTDSSRLEASIYRTDLKDAIIAADGNP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHCCCCEEEECCCCC
PENVASARINGFEATLKQELFGWQSNLGVAIIDPRDRDTGHTLARRARRTLSWDLDRQFD
CHHHHHHHCCCHHHHHHHHHHCHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHH
RLGLGASWQAVSSSYDDLNNQQPLGGYALLGLRSSWALNREIKLDFKIDNLLDKGYSRAL
HHCCCCCHHHHHCCHHHCCCCCCCCCEEEEEECCCCEECCEEEEEEEHHHHHHHHHHHHH
YSHDGSQYDYREEGRAFMFGVTWTPQL
HCCCCCCCCHHHCCCEEEEEEECCCCC
>Mature Secondary Structure
MNLSRLALPLLLLPSANALADNFERDQALKLPDVLISANRQVEARNDSSAANTVFTREDI
CCHHHHHHHEEEECCCHHHHHCCCCCCCCCCCHHHHCCCCEEECCCCCCHHHEEEEHHHH
ERLQPRSVTDLLQRVPGVQVAQTGGRGSLPGIYIRGTQSAQSLVLVDGQRIGNSTSGDSN
HHCCCCCHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEECHHCCCCCCCCCC
LQHLNIEQIERVEVLRGSRSVIYGSDAIGGVIQIFTRRGGEQGLQPRMHVGFGSNQTWER
CEECCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHH
SIGLSGGDEKTRFNLGASLDETAGLDRTHESYPSDSDHDAYRNKSISLSLSHALTDDIEV
HCCCCCCCCCEEEECCCCCHHHCCCCCCHHCCCCCCCCHHHCCCEEEEEEHHHHCCHHHH
GANLLDNRGKSEFDSPWGRYVEATDRSYEEQPYSDFTVSSFSSYIDARINERWRSRLELG
HHHHHHCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
HSENREKTFDKLSDERSVFNTYRDSVTWQNDLTLDARNSLILGGNWYEDRINSSTAFDED
CCCCHHHHHHHHHHHHHHHHHHHHCCEECCCEEEECCCCEEECCCCHHHHCCCCCCCCCC
SRWNRAAFIQHRYQADSFSTELGLRRDDNQQFGGQNSWSGTLTLPVNPDNDLLLSYSEGF
CCCCHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCC
RAPTFNDLYYPKYSNPDLKPETSKSYELQWRSQLTDSSRLEASIYRTDLKDAIIAADGNP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHCCCCEEEECCCCC
PENVASARINGFEATLKQELFGWQSNLGVAIIDPRDRDTGHTLARRARRTLSWDLDRQFD
CHHHHHHHCCCHHHHHHHHHHCHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHH
RLGLGASWQAVSSSYDDLNNQQPLGGYALLGLRSSWALNREIKLDFKIDNLLDKGYSRAL
HHCCCCCHHHHHCCHHHCCCCCCCCCEEEEEECCCCEECCEEEEEEEHHHHHHHHHHHHH
YSHDGSQYDYREEGRAFMFGVTWTPQL
HCCCCCCCCHHHCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA