Click here to switch to the map view.

The map label for this gene is rluE [H]

Identifier: 77461162

GI number: 77461162

Start: 5566740

End: 5567375

Strand: Reverse

Name: rluE [H]

Synonym: Pfl01_4941

Alternate gene names: 77461162

Gene position: 5567375-5566740 (Counterclockwise)

Preceding gene: 77461163

Following gene: 77461159

Centisome position: 86.47

GC content: 64.47

Gene sequence:

>636_bases
ATGTCCCGTCCCCAACGTCCGCCTTCCCGCCGCCCCGGCGCGAAGCCAGCTTCTTCAGCCCCGCGCCGTGTCGCCAAGGC
ACCGCCCGCTGAGCCGAAGCTGATCCTGTTCAACAAACCGTTCGATGTGCTGACGCAATTCAGCGACGGTGAAGGGCGGG
CGACGCTCAAGGATTACATCGACATTCCGGGTATTTACCCGGCCGGACGGCTGGACCGCGACAGCGAGGGTTTGCTGCTG
CTGACCAACGACGGTCAGTTGCAGGCGCGGATCGCCGACCCCAGGCACAAACTGGCGAAAACCTATTGGGTGCAGGTCGA
AGGCCTGCCTACGGCCGAGCAGTTGCAGCGTCTGCGTGACGGCGTTGAATTGAATGACGGCATGACCTTGCCCGCGCGAG
CGAGGCAACTGGACGAGCCCGAACTGTGGCCGCGCAATCCACCGGTGCGCTTTCGCGCAACGATTCCGACAACCTGGCTG
GAACTGGTGATTCGCGAAGGGCGCAACCGCCAGGTGCGGCGGATGACGGCGGCGGTCGGGCTGCCGACGTTGCGGCTGGT
GCGGGTCAGGATTGGTGACTGGACGATCGAAGGGCTCGATCAGGGCCAGTGGAAAGAAGTGCCGGCGCGCTTATAA

Upstream 100 bases:

>100_bases
CAGCTTCGACGAGCCGCCGTTCCGCTAACGGTTTGTCGTGGTCATTTGGCGGTCGGGGCACTAGCATTGTCAGCCCTGAT
CGCTAAATGTCTTCTCTCTT

Downstream 100 bases:

>100_bases
GGCGCCGGATTCGATCAGGCCGATCACCACGCTTTTGATGATGAACGCGGCCACACCCAGGCCCAGCACGAAGAACAGAA
TGAACGAGCCGAAGCGTCCG

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSRPQRPPSRRPGAKPASSAPRRVAKAPPAEPKLILFNKPFDVLTQFSDGEGRATLKDYIDIPGIYPAGRLDRDSEGLLL
LTNDGQLQARIADPRHKLAKTYWVQVEGLPTAEQLQRLRDGVELNDGMTLPARARQLDEPELWPRNPPVRFRATIPTTWL
ELVIREGRNRQVRRMTAAVGLPTLRLVRVRIGDWTIEGLDQGQWKEVPARL

Sequences:

>Translated_211_residues
MSRPQRPPSRRPGAKPASSAPRRVAKAPPAEPKLILFNKPFDVLTQFSDGEGRATLKDYIDIPGIYPAGRLDRDSEGLLL
LTNDGQLQARIADPRHKLAKTYWVQVEGLPTAEQLQRLRDGVELNDGMTLPARARQLDEPELWPRNPPVRFRATIPTTWL
ELVIREGRNRQVRRMTAAVGLPTLRLVRVRIGDWTIEGLDQGQWKEVPARL
>Mature_210_residues
SRPQRPPSRRPGAKPASSAPRRVAKAPPAEPKLILFNKPFDVLTQFSDGEGRATLKDYIDIPGIYPAGRLDRDSEGLLLL
TNDGQLQARIADPRHKLAKTYWVQVEGLPTAEQLQRLRDGVELNDGMTLPARARQLDEPELWPRNPPVRFRATIPTTWLE
LVIREGRNRQVRRMTAAVGLPTLRLVRVRIGDWTIEGLDQGQWKEVPARL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=191, Percent_Identity=58.6387434554974, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1788510, Length=170, Percent_Identity=33.5294117647059, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787524, Length=182, Percent_Identity=34.6153846153846, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1790453, Length=175, Percent_Identity=30.8571428571429, Blast_Score=76, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 23871; Mature: 23740

Theoretical pI: Translated: 11.09; Mature: 11.09

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPQRPPSRRPGAKPASSAPRRVAKAPPAEPKLILFNKPFDVLTQFSDGEGRATLKDYI
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCHHHHEECCCCCCCEEHHHHC
DIPGIYPAGRLDRDSEGLLLLTNDGQLQARIADPRHKLAKTYWVQVEGLPTAEQLQRLRD
CCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHCEEEEEEECCCCCHHHHHHHHC
GVELNDGMTLPARARQLDEPELWPRNPPVRFRATIPTTWLELVIREGRNRQVRRMTAAVG
CCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHC
LPTLRLVRVRIGDWTIEGLDQGQWKEVPARL
CCCEEEEEEEECCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SRPQRPPSRRPGAKPASSAPRRVAKAPPAEPKLILFNKPFDVLTQFSDGEGRATLKDYI
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCHHHHEECCCCCCCEEHHHHC
DIPGIYPAGRLDRDSEGLLLLTNDGQLQARIADPRHKLAKTYWVQVEGLPTAEQLQRLRD
CCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHCEEEEEEECCCCCHHHHHHHHC
GVELNDGMTLPARARQLDEPELWPRNPPVRFRATIPTTWLELVIREGRNRQVRRMTAAVG
CCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHC
LPTLRLVRVRIGDWTIEGLDQGQWKEVPARL
CCCEEEEEEEECCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]