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The map label for this gene is trmA [H]

Identifier: 77461119

GI number: 77461119

Start: 5522165

End: 5523244

Strand: Direct

Name: trmA [H]

Synonym: Pfl01_4898

Alternate gene names: 77461119

Gene position: 5522165-5523244 (Clockwise)

Preceding gene: 77461118

Following gene: 77461122

Centisome position: 85.77

GC content: 62.59

Gene sequence:

>1080_bases
ATGACTTTTGATTCCCAGGCCTACGCCGCACAACTCGAAGACAAGGTCACGCGCCTGCGTGACCTGCTGGCCCCGTTCGA
TGCACCGGAGCCGACGGTGTTCGACTCGCCGCTGCAGAACTTCCGCCTGCGCGCCGAATTCCGCCTGTGGCGCGAGGGGG
GTGAGCGGCATTACGCGATGTTCTCGCAGGACGACAAACGCACGCCGATCCTGATCGAAGAGTTCCCGATCGCCAGCCTG
CGCATCAACCAGTTGATGCCGCAATTGAAGGCGGCGTGGCAGGCCAGCGCAGCGCTGAGCCACAAGTTGTTCCAGGTGGA
GTTTCTGACCACCCTGGCCGGCGACGCAATGATCACCCTGTGCTATCACCGTCCGCTGGACGAGCACTGGCATGCGGCGG
CGACCAAACTGGCGACGGATCTGGGCGTCAGCATCATCGGTCGTTCCAAGGGCAAGCGCGAAGTGCTCGGCCTCGACTAC
GTGGTCGAGAAACTCGACGTCGGCGGTCGCACATTCAGCTATCGCCAACCGGAAGGCGCGTTCACCCAGCCCAACGGCAC
CGTCAACCAGAAGATGCTCAACTGGGCCTACGAAGCACTGGGCGATCGCAGCGACGATCTGCTGGAGCTTTATTGCGGCA
ACGGCAACTTCACCCTGCCGCTGGCAACCCGCGTGCGCAAAGTGCTGGCCACCGAAATCAGCAAGACTTCGGTCAACGCC
GCACTGAGCAACCTGGCCGAAAACGCTGTGGATAACGTCACCTTGGTGCGCCTGTCCGCCGAAGAGCTGACCGAAGCCCT
GAACGAAGTTCGCCCGTTCCGTCGCCTGCACGGCATCGATCTGAAAAGCTACGAATTCGGCAGCGTGTTCGTCGACCCGC
CACGGGCCGGCATGGACCCGGACACCTGCGAGCTGACCCGGCGCTTCGACAACATCCTGTACATCTCCTGCAACCCGGAA
ACGCTGGCGGCCAACATTGCCCAACTGCACGACACGCACCGCATCACCCGCTGCGCACTGTTCGACCAGTTCCCGTGGAC
TCACCACATGGAATCCGGCGTGCTGCTGACCCGACGTTGA

Upstream 100 bases:

>100_bases
GGACTGCGATCAAACTGCTGTCCGGCCGCGCCCGTGAGCTGAACCCGGCGCTGGTGATCCTGTCGATTCTGTTCGTGATC
AAGCTGGGTTGGTTCAACGC

Downstream 100 bases:

>100_bases
TCGCAGGTGCCAGAAACAAGAAAGCCGTCCTCATCGACGGCTTTTTTGTGCCTGTTCATGGCACGGGATCGATCTTGCGC
GGACGCCCGCCCTTCCTGCC

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTFDSQAYAAQLEDKVTRLRDLLAPFDAPEPTVFDSPLQNFRLRAEFRLWREGGERHYAMFSQDDKRTPILIEEFPIASL
RINQLMPQLKAAWQASAALSHKLFQVEFLTTLAGDAMITLCYHRPLDEHWHAAATKLATDLGVSIIGRSKGKREVLGLDY
VVEKLDVGGRTFSYRQPEGAFTQPNGTVNQKMLNWAYEALGDRSDDLLELYCGNGNFTLPLATRVRKVLATEISKTSVNA
ALSNLAENAVDNVTLVRLSAEELTEALNEVRPFRRLHGIDLKSYEFGSVFVDPPRAGMDPDTCELTRRFDNILYISCNPE
TLAANIAQLHDTHRITRCALFDQFPWTHHMESGVLLTRR

Sequences:

>Translated_359_residues
MTFDSQAYAAQLEDKVTRLRDLLAPFDAPEPTVFDSPLQNFRLRAEFRLWREGGERHYAMFSQDDKRTPILIEEFPIASL
RINQLMPQLKAAWQASAALSHKLFQVEFLTTLAGDAMITLCYHRPLDEHWHAAATKLATDLGVSIIGRSKGKREVLGLDY
VVEKLDVGGRTFSYRQPEGAFTQPNGTVNQKMLNWAYEALGDRSDDLLELYCGNGNFTLPLATRVRKVLATEISKTSVNA
ALSNLAENAVDNVTLVRLSAEELTEALNEVRPFRRLHGIDLKSYEFGSVFVDPPRAGMDPDTCELTRRFDNILYISCNPE
TLAANIAQLHDTHRITRCALFDQFPWTHHMESGVLLTRR
>Mature_358_residues
TFDSQAYAAQLEDKVTRLRDLLAPFDAPEPTVFDSPLQNFRLRAEFRLWREGGERHYAMFSQDDKRTPILIEEFPIASLR
INQLMPQLKAAWQASAALSHKLFQVEFLTTLAGDAMITLCYHRPLDEHWHAAATKLATDLGVSIIGRSKGKREVLGLDYV
VEKLDVGGRTFSYRQPEGAFTQPNGTVNQKMLNWAYEALGDRSDDLLELYCGNGNFTLPLATRVRKVLATEISKTSVNAA
LSNLAENAVDNVTLVRLSAEELTEALNEVRPFRRLHGIDLKSYEFGSVFVDPPRAGMDPDTCELTRRFDNILYISCNPET
LAANIAQLHDTHRITRCALFDQFPWTHHMESGVLLTRR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790403, Length=360, Percent_Identity=49.1666666666667, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789148, Length=295, Percent_Identity=31.864406779661, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787083, Length=157, Percent_Identity=29.9363057324841, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 40615; Mature: 40483

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDSQAYAAQLEDKVTRLRDLLAPFDAPEPTVFDSPLQNFRLRAEFRLWREGGERHYAM
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEHHHHHHHHHCCCEEEEE
FSQDDKRTPILIEEFPIASLRINQLMPQLKAAWQASAALSHKLFQVEFLTTLAGDAMITL
ECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CYHRPLDEHWHAAATKLATDLGVSIIGRSKGKREVLGLDYVVEKLDVGGRTFSYRQPEGA
EECCCCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCC
FTQPNGTVNQKMLNWAYEALGDRSDDLLELYCGNGNFTLPLATRVRKVLATEISKTSVNA
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHH
ALSNLAENAVDNVTLVRLSAEELTEALNEVRPFRRLHGIDLKSYEFGSVFVDPPRAGMDP
HHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEECCCCCCCCC
DTCELTRRFDNILYISCNPETLAANIAQLHDTHRITRCALFDQFPWTHHMESGVLLTRR
HHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
TFDSQAYAAQLEDKVTRLRDLLAPFDAPEPTVFDSPLQNFRLRAEFRLWREGGERHYAM
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEHHHHHHHHHCCCEEEEE
FSQDDKRTPILIEEFPIASLRINQLMPQLKAAWQASAALSHKLFQVEFLTTLAGDAMITL
ECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CYHRPLDEHWHAAATKLATDLGVSIIGRSKGKREVLGLDYVVEKLDVGGRTFSYRQPEGA
EECCCCHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCC
FTQPNGTVNQKMLNWAYEALGDRSDDLLELYCGNGNFTLPLATRVRKVLATEISKTSVNA
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHH
ALSNLAENAVDNVTLVRLSAEELTEALNEVRPFRRLHGIDLKSYEFGSVFVDPPRAGMDP
HHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEECCCCCCCCC
DTCELTRRFDNILYISCNPETLAANIAQLHDTHRITRCALFDQFPWTHHMESGVLLTRR
HHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10601212 [H]