Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 77461095

GI number: 77461095

Start: 5492571

End: 5493908

Strand: Reverse

Name: gdhA [H]

Synonym: Pfl01_4874

Alternate gene names: 77461095

Gene position: 5493908-5492571 (Counterclockwise)

Preceding gene: 77461096

Following gene: 77461090

Centisome position: 85.33

GC content: 61.36

Gene sequence:

>1338_bases
ATGATCGAATCCGTCGAATCCTTCCTCGCCCGTCTGAAAAAACGCGATCCGGATCAACCCGAATTCCACCAGGCCGTCGA
AGAAGTCCTGCGCAGTCTGTGGCCGTTTCTCGAGGCCAATCCGCACTATCTGACCTCGGGAATTCTGGAGCGTATCTGCG
AGCCGGAGCGGGCGGTGGTGTTCCGAGTGTCGTGGGTCGACGATCAGGGCAGGGTCCAGGTCAATCGCGGTTTCCGCATC
CAGATGAACAGCGCCATTGGCCCCTACAAGGGCGGTTTGCGTTTCCATCCTTCGGTGAATCTGGGCGTGTTGAAATTCCT
CGCCTTCGAGCAGACCTTCAAGAATTCCCTGACCTCGCTGCCCATGGGCGGAGGCAAGGGTGGCTCGGACTTCGATCCGA
AGGGCAAGAGCGACGCCGAAGTCATGCGTTTTTGCCAGGCATTCATGAGCGAGCTGTACCGCCACATCGGTGCCGACGTG
GACGTCCCGGCCGGTGATATCGGTGTCGGCGCACGGGAAATCGGTTTCCTGTTCGGCCAGTACAAACGCCTGAGCAACCA
GTTCACCAGCGTGCTGACCGGCAAGGGCATGACCTACGGCGGCAGCTTGATTCGCCCCGAAGCCACCGGTTTCGGTTGCG
TGTACTTCGCCGAGGAAATGCTCAAGCGTCGCGAGCAGACCGTCGAAGGCAAACGCGTGGCGATCTCCGGTTCCGGCAAC
GTTGCGCAATACGCGGCTCGCAAAGTGATGGATCTGGGCGGCAAGGTGATTTCCCTCTCCGACTCCGAAGGCACGCTGTA
TTGCGAGGCGGGCCTGAGCGAAGAACAGTGGTTGGCGCTGCTGGAACTGAAGAACGTCAAGCGTGGGCGGATCAGCGAAT
TGGCGTCTGCCTTCGGTCTGGAGTTCCGCGCCGGTCAGTTGCCATGGTCGTTGCCGTGCGACATTGCGCTGCCATGCGCG
ACCCAGAACGAACTTGACGCGGAATCCGCCCGCACCTTGCTGCGCAACGGCTGCGTCTGCGTGGCCGAAGGCGCGAACAT
GCCGACCACCCTTGAGGCTGTGGATATCTTTATCGAAGCGGGCATTCTGTTCGCGCCGGGCAAGGCCTCGAATGCCGGCG
GCGTAGCGGTCAGTGGGCTGGAGATGTCGCAGAACGCCATGCGCCTGCTGTGGACGGGCGGCGAGGTGGACAGCAAGCTG
CACGGGATCATGCAGTCGATCCACCACGCCTGCGTGCATTACGGTGAGGAAAACGGCCGGATCAACTACGTCAAAGGCGC
GAACATCGCCGGCTTCGTCAAGGTCGCCGACGCGATGCTCGCCCAAGGCGTGGTTTAA

Upstream 100 bases:

>100_bases
TTCATAAATGCGACATTTTTGTCTGTTGCTGTGTACATATCGTTTGTTACAGTCCGGCTCAATCTCCTCCAACGACAACA
AATCTGCCGAGACTTTTCCC

Downstream 100 bases:

>100_bases
GAACGCTCTATGCGGATCACTTCAATCACTTGATCGCCGGCCGGGCGCTGCCACAGCACTTCATCGTTGAGCCGCGCGCC
AAGCAGCGCACGGCCCAGCG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MIESVESFLARLKKRDPDQPEFHQAVEEVLRSLWPFLEANPHYLTSGILERICEPERAVVFRVSWVDDQGRVQVNRGFRI
QMNSAIGPYKGGLRFHPSVNLGVLKFLAFEQTFKNSLTSLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADV
DVPAGDIGVGAREIGFLFGQYKRLSNQFTSVLTGKGMTYGGSLIRPEATGFGCVYFAEEMLKRREQTVEGKRVAISGSGN
VAQYAARKVMDLGGKVISLSDSEGTLYCEAGLSEEQWLALLELKNVKRGRISELASAFGLEFRAGQLPWSLPCDIALPCA
TQNELDAESARTLLRNGCVCVAEGANMPTTLEAVDIFIEAGILFAPGKASNAGGVAVSGLEMSQNAMRLLWTGGEVDSKL
HGIMQSIHHACVHYGEENGRINYVKGANIAGFVKVADAMLAQGVV

Sequences:

>Translated_445_residues
MIESVESFLARLKKRDPDQPEFHQAVEEVLRSLWPFLEANPHYLTSGILERICEPERAVVFRVSWVDDQGRVQVNRGFRI
QMNSAIGPYKGGLRFHPSVNLGVLKFLAFEQTFKNSLTSLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADV
DVPAGDIGVGAREIGFLFGQYKRLSNQFTSVLTGKGMTYGGSLIRPEATGFGCVYFAEEMLKRREQTVEGKRVAISGSGN
VAQYAARKVMDLGGKVISLSDSEGTLYCEAGLSEEQWLALLELKNVKRGRISELASAFGLEFRAGQLPWSLPCDIALPCA
TQNELDAESARTLLRNGCVCVAEGANMPTTLEAVDIFIEAGILFAPGKASNAGGVAVSGLEMSQNAMRLLWTGGEVDSKL
HGIMQSIHHACVHYGEENGRINYVKGANIAGFVKVADAMLAQGVV
>Mature_445_residues
MIESVESFLARLKKRDPDQPEFHQAVEEVLRSLWPFLEANPHYLTSGILERICEPERAVVFRVSWVDDQGRVQVNRGFRI
QMNSAIGPYKGGLRFHPSVNLGVLKFLAFEQTFKNSLTSLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADV
DVPAGDIGVGAREIGFLFGQYKRLSNQFTSVLTGKGMTYGGSLIRPEATGFGCVYFAEEMLKRREQTVEGKRVAISGSGN
VAQYAARKVMDLGGKVISLSDSEGTLYCEAGLSEEQWLALLELKNVKRGRISELASAFGLEFRAGQLPWSLPCDIALPCA
TQNELDAESARTLLRNGCVCVAEGANMPTTLEAVDIFIEAGILFAPGKASNAGGVAVSGLEMSQNAMRLLWTGGEVDSKL
HGIMQSIHHACVHYGEENGRINYVKGANIAGFVKVADAMLAQGVV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=400, Percent_Identity=30.25, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI4885281, Length=400, Percent_Identity=30.25, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1788059, Length=442, Percent_Identity=65.6108597285068, Blast_Score=623, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI17544676, Length=362, Percent_Identity=29.5580110497238, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324951, Length=454, Percent_Identity=50.6607929515419, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319256, Length=456, Percent_Identity=50.8771929824561, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24649283, Length=437, Percent_Identity=28.3752860411899, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI45553475, Length=332, Percent_Identity=31.9277108433735, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45549226, Length=332, Percent_Identity=31.9277108433735, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24649460, Length=288, Percent_Identity=31.9444444444444, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48359; Mature: 48359

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIESVESFLARLKKRDPDQPEFHQAVEEVLRSLWPFLEANPHYLTSGILERICEPERAVV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEE
FRVSWVDDQGRVQVNRGFRIQMNSAIGPYKGGLRFHPSVNLGVLKFLAFEQTFKNSLTSL
EEEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
PMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADVDVPAGDIGVGAREIGFLFGQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
YKRLSNQFTSVLTGKGMTYGGSLIRPEATGFGCVYFAEEMLKRREQTVEGKRVAISGSGN
HHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCC
VAQYAARKVMDLGGKVISLSDSEGTLYCEAGLSEEQWLALLELKNVKRGRISELASAFGL
HHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
EFRAGQLPWSLPCDIALPCATQNELDAESARTLLRNGCVCVAEGANMPTTLEAVDIFIEA
EEECCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHC
GILFAPGKASNAGGVAVSGLEMSQNAMRLLWTGGEVDSKLHGIMQSIHHACVHYGEENGR
CEEECCCCCCCCCCEEEECCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCE
INYVKGANIAGFVKVADAMLAQGVV
EEEEECCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIESVESFLARLKKRDPDQPEFHQAVEEVLRSLWPFLEANPHYLTSGILERICEPERAVV
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEE
FRVSWVDDQGRVQVNRGFRIQMNSAIGPYKGGLRFHPSVNLGVLKFLAFEQTFKNSLTSL
EEEEEECCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
PMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADVDVPAGDIGVGAREIGFLFGQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
YKRLSNQFTSVLTGKGMTYGGSLIRPEATGFGCVYFAEEMLKRREQTVEGKRVAISGSGN
HHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCC
VAQYAARKVMDLGGKVISLSDSEGTLYCEAGLSEEQWLALLELKNVKRGRISELASAFGL
HHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
EFRAGQLPWSLPCDIALPCATQNELDAESARTLLRNGCVCVAEGANMPTTLEAVDIFIEA
EEECCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHC
GILFAPGKASNAGGVAVSGLEMSQNAMRLLWTGGEVDSKLHGIMQSIHHACVHYGEENGR
CEEECCCCCCCCCCEEEECCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCE
INYVKGANIAGFVKVADAMLAQGVV
EEEEECCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]