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The map label for this gene is pys2 [H]

Identifier: 77460980

GI number: 77460980

Start: 5364692

End: 5365939

Strand: Direct

Name: pys2 [H]

Synonym: Pfl01_4759

Alternate gene names: 77460980

Gene position: 5364692-5365939 (Clockwise)

Preceding gene: 77460979

Following gene: 77460981

Centisome position: 83.32

GC content: 59.38

Gene sequence:

>1248_bases
ATGAACCTGAACAAATTCCTTTTCGACGATGAGTGGCGTCGAAAACGCCCGAGCGAAAACGCGACATGGACCGGATGGAT
TCCACCCCCGCCACCAGAACCCGTTTATATCCGGACAGACGAATGGCCCACCCCGACGCCCCGCGACGATCTGGTATTTG
CCAAATCCTGCACACCGGATAACTGGTGCCGGACGGATGCCGGCACAACACCTGAGCCCGCCTCTCACTTTGGCAAAGTC
ATGGTGGCAGGCGCCCTGCGCATTCCCGCTGCCAGTCACGCCGTAGCGAAGGCCATCGGCGCCGATCTGGCGTTGGGGCG
CATGGCCGGTGGTGGAATCCTGCAGCAGCGCCTTAATTGGGCAATCCGCGGTGCGGGAGGCCCGGCCAGCGTGTTTATCC
TCGGGATGCTCCCGACCCGAATGGGCGACGGCACGCTTCACACTGATGAACAGCTACGCAGCATGAGCCGCGCCACCACT
CGCGTGCGGTTCCAGTTCCGACGCGATGCCGAAGGCGTCATGCAGGTTTACGGCATCCACACCGGAGCCTCGGGCGACGA
CTCGGTGCGTACCGTAAAAGTCGAGTGGAACAACGACAAGAGCGCCATGGAGGCGAAGCTCAACGACATCACGATTTTGT
GGACGCCGCAACGCGGGCCGCTGGGATCGATGCCGCCGCTGGTTCATCCGGAGCACGGCGAAGCACTGAGTACGATCCTC
GTCCACCCGATCGCCGCGAACACCGACAGCCAGATTGAAATCCTGCCCGGCGAAGACATCACCGCCGAGGATTGTATTCT
GGTGTTCCCGGCGGAGACCGGGCTGAAGTCGTTGTATGTGGTGTTTTCGAAACCGGCGAGATTGATGCCGGGGACGGTGA
CGGGGGTTGGGGAGGATGTTTCCGGTGTATGGCTTGACCATGCCCGGGGGGGATCTGGTGCGCCAATCCCAACCAGCATT
GCCGATTCGCTAAGAGGGCGTGAATATTCCAGCTTTGATTCATTCAGGCGCGCCTTCTGGGCCGAAGCATCAAAAGATAC
CAGGCTAGCCGGGCAACTCAGCGAAGATAGCTTGGAGCGTATGCGCAACGGAAAGGCGCCTCGCGCCCGATTAGCTGATG
CGGCTGGTAAAAGAATCTCTCACGAAATTCATCATGTGGAACTGATATCTCAAGGCGGCGAGGTCTATAACGTTGACAAC
CTGAGGGTCCACACTCCTAAAAATCACGTCGAAATACACCAAGATTGA

Upstream 100 bases:

>100_bases
GTTCGTCCAGGCACCTGCATCTGCGCCAGTCGATTTGATCGCCATCCCGATTGACCGACACGCTCCCCTGTCTCGATTCC
AGGACAGACAGGGAGTGGTC

Downstream 100 bases:

>100_bases
GGCAACTGCATGAGCGACCCCTTCATCAAAAGCAATTTCTCTGATTACACCGAACAAGACTTCGTTGAGTTCATTGACGA
GATCAGAAAGGAAGACAAGG

Product: S-type Pyocin

Products: NA

Alternate protein names: Killer protein [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNLNKFLFDDEWRRKRPSENATWTGWIPPPPPEPVYIRTDEWPTPTPRDDLVFAKSCTPDNWCRTDAGTTPEPASHFGKV
MVAGALRIPAASHAVAKAIGADLALGRMAGGGILQQRLNWAIRGAGGPASVFILGMLPTRMGDGTLHTDEQLRSMSRATT
RVRFQFRRDAEGVMQVYGIHTGASGDDSVRTVKVEWNNDKSAMEAKLNDITILWTPQRGPLGSMPPLVHPEHGEALSTIL
VHPIAANTDSQIEILPGEDITAEDCILVFPAETGLKSLYVVFSKPARLMPGTVTGVGEDVSGVWLDHARGGSGAPIPTSI
ADSLRGREYSSFDSFRRAFWAEASKDTRLAGQLSEDSLERMRNGKAPRARLADAAGKRISHEIHHVELISQGGEVYNVDN
LRVHTPKNHVEIHQD

Sequences:

>Translated_415_residues
MNLNKFLFDDEWRRKRPSENATWTGWIPPPPPEPVYIRTDEWPTPTPRDDLVFAKSCTPDNWCRTDAGTTPEPASHFGKV
MVAGALRIPAASHAVAKAIGADLALGRMAGGGILQQRLNWAIRGAGGPASVFILGMLPTRMGDGTLHTDEQLRSMSRATT
RVRFQFRRDAEGVMQVYGIHTGASGDDSVRTVKVEWNNDKSAMEAKLNDITILWTPQRGPLGSMPPLVHPEHGEALSTIL
VHPIAANTDSQIEILPGEDITAEDCILVFPAETGLKSLYVVFSKPARLMPGTVTGVGEDVSGVWLDHARGGSGAPIPTSI
ADSLRGREYSSFDSFRRAFWAEASKDTRLAGQLSEDSLERMRNGKAPRARLADAAGKRISHEIHHVELISQGGEVYNVDN
LRVHTPKNHVEIHQD
>Mature_415_residues
MNLNKFLFDDEWRRKRPSENATWTGWIPPPPPEPVYIRTDEWPTPTPRDDLVFAKSCTPDNWCRTDAGTTPEPASHFGKV
MVAGALRIPAASHAVAKAIGADLALGRMAGGGILQQRLNWAIRGAGGPASVFILGMLPTRMGDGTLHTDEQLRSMSRATT
RVRFQFRRDAEGVMQVYGIHTGASGDDSVRTVKVEWNNDKSAMEAKLNDITILWTPQRGPLGSMPPLVHPEHGEALSTIL
VHPIAANTDSQIEILPGEDITAEDCILVFPAETGLKSLYVVFSKPARLMPGTVTGVGEDVSGVWLDHARGGSGAPIPTSI
ADSLRGREYSSFDSFRRAFWAEASKDTRLAGQLSEDSLERMRNGKAPRARLADAAGKRISHEIHHVELISQGGEVYNVDN
LRVHTPKNHVEIHQD

Specific function: Causes breakdown of chromosomal DNA as well as complete inhibition of lipid synthesis in sensitive cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicin/pyosin nuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR003060
- InterPro:   IPR010715
- InterPro:   IPR016128 [H]

Pfam domain/function: PF06958 Pyocin_S [H]

EC number: NA

Molecular weight: Translated: 45422; Mature: 45422

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNKFLFDDEWRRKRPSENATWTGWIPPPPPEPVYIRTDEWPTPTPRDDLVFAKSCTPD
CCCCCCCCCCHHHHCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCC
NWCRTDAGTTPEPASHFGKVMVAGALRIPAASHAVAKAIGADLALGRMAGGGILQQRLNW
CCCCCCCCCCCCCHHHHCCEEEECEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCE
AIRGAGGPASVFILGMLPTRMGDGTLHTDEQLRSMSRATTRVRFQFRRDAEGVMQVYGIH
EEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCHHHHEEEEEEE
TGASGDDSVRTVKVEWNNDKSAMEAKLNDITILWTPQRGPLGSMPPLVHPEHGEALSTIL
CCCCCCCCEEEEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHE
VHPIAANTDSQIEILPGEDITAEDCILVFPAETGLKSLYVVFSKPARLMPGTVTGVGEDV
EEEEECCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEECCCCCC
SGVWLDHARGGSGAPIPTSIADSLRGREYSSFDSFRRAFWAEASKDTRLAGQLSEDSLER
CEEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHH
MRNGKAPRARLADAAGKRISHEIHHVELISQGGEVYNVDNLRVHTPKNHVEIHQD
HHCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEECCCEEEECCCCCEEEECC
>Mature Secondary Structure
MNLNKFLFDDEWRRKRPSENATWTGWIPPPPPEPVYIRTDEWPTPTPRDDLVFAKSCTPD
CCCCCCCCCCHHHHCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCC
NWCRTDAGTTPEPASHFGKVMVAGALRIPAASHAVAKAIGADLALGRMAGGGILQQRLNW
CCCCCCCCCCCCCHHHHCCEEEECEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCE
AIRGAGGPASVFILGMLPTRMGDGTLHTDEQLRSMSRATTRVRFQFRRDAEGVMQVYGIH
EEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCHHHHEEEEEEE
TGASGDDSVRTVKVEWNNDKSAMEAKLNDITILWTPQRGPLGSMPPLVHPEHGEALSTIL
CCCCCCCCEEEEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHE
VHPIAANTDSQIEILPGEDITAEDCILVFPAETGLKSLYVVFSKPARLMPGTVTGVGEDV
EEEEECCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEECCCCCC
SGVWLDHARGGSGAPIPTSIADSLRGREYSSFDSFRRAFWAEASKDTRLAGQLSEDSLER
CEEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHH
MRNGKAPRARLADAAGKRISHEIHHVELISQGGEVYNVDNLRVHTPKNHVEIHQD
HHCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEECCCEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491711; 10984043 [H]