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The map label for this gene is lolB

Identifier: 77460972

GI number: 77460972

Start: 5355408

End: 5356025

Strand: Direct

Name: lolB

Synonym: Pfl01_4751

Alternate gene names: 77460972

Gene position: 5355408-5356025 (Clockwise)

Preceding gene: 77460971

Following gene: 77460973

Centisome position: 83.18

GC content: 63.92

Gene sequence:

>618_bases
ATGTTTTTGCGCCACGTCATCGTTTTCAGCTTCATCGCCCTGCTCGCCGGTTGCGCGGGCTTCGGCGCCCGTGAATCGGT
CGAAGGCCACGGCAATCCGGCACAGTGGGCCGCGAACAAACAACACCTGACCGGCCTCGACGGCTGGCAGATCGACGGCA
AGATCGGCATCCGCGCCCCGAAAGATTCGGGCAGCGGCACGCTGTTCTGGCTGCAACGTCAGGATTACTACGACATCCGC
CTGTCCGGCCCGCTGGGTCGTGGCGCGGCGCGACTGACCGGCCGTCCGGGCGCGGTGTCGCTGGAAGTGGCGAATCAGGG
CCGTTATGAAGCGCCGACTCCGGAAGCACTGGTCGAAGAGCAACTGGGCTGGAAACTGCCGGTCTCGCATCTGGCGTGGT
GGGTTCGCGGCCTCCCGGCACCGGACAGCAAAAGCCGTCTGACCCTGGACGGCAACAGCCGCCTGGCCAATCTGGATCAG
GATGGCTGGCAGGTTGAATACCTCAGCTATGCCGAACAGAACGGTTACTGGCTGCCCGAGCGGATCAAGCTGCACGGCAG
CGATCTGGACGTGACGCTGGTGATCAAGACCTGGCAACCGCGCAAACTGGGGCAATAA

Upstream 100 bases:

>100_bases
AGCGAAACAAATCTGGGGCAAGTTCCTCAAGGACCAGCCTGACAGCACCATCCTGCGCAGCACCATCAAGCGCCTGACCG
GATCCGAGACTCTTTAACTC

Downstream 100 bases:

>100_bases
CGCATGACCGCTCCACGCCTCACTTTGCCTTCACCGGCCAAACTCAACCTGATGTTGCACATTCTCGGTCGCCGTGAAGA
CGGTTATCACGAATTGCAGA

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MFLRHVIVFSFIALLAGCAGFGARESVEGHGNPAQWAANKQHLTGLDGWQIDGKIGIRAPKDSGSGTLFWLQRQDYYDIR
LSGPLGRGAARLTGRPGAVSLEVANQGRYEAPTPEALVEEQLGWKLPVSHLAWWVRGLPAPDSKSRLTLDGNSRLANLDQ
DGWQVEYLSYAEQNGYWLPERIKLHGSDLDVTLVIKTWQPRKLGQ

Sequences:

>Translated_205_residues
MFLRHVIVFSFIALLAGCAGFGARESVEGHGNPAQWAANKQHLTGLDGWQIDGKIGIRAPKDSGSGTLFWLQRQDYYDIR
LSGPLGRGAARLTGRPGAVSLEVANQGRYEAPTPEALVEEQLGWKLPVSHLAWWVRGLPAPDSKSRLTLDGNSRLANLDQ
DGWQVEYLSYAEQNGYWLPERIKLHGSDLDVTLVIKTWQPRKLGQ
>Mature_205_residues
MFLRHVIVFSFIALLAGCAGFGARESVEGHGNPAQWAANKQHLTGLDGWQIDGKIGIRAPKDSGSGTLFWLQRQDYYDIR
LSGPLGRGAARLTGRPGAVSLEVANQGRYEAPTPEALVEEQLGWKLPVSHLAWWVRGLPAPDSKSRLTLDGNSRLANLDQ
DGWQVEYLSYAEQNGYWLPERIKLHGSDLDVTLVIKTWQPRKLGQ

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

Organism=Escherichia coli, GI1787460, Length=203, Percent_Identity=27.0935960591133, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_PSEPF (Q3K6W6)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350479.1
- ProteinModelPortal:   Q3K6W6
- SMR:   Q3K6W6
- STRING:   Q3K6W6
- GeneID:   3715172
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4751
- eggNOG:   COG3017
- HOGENOM:   HBG645031
- OMA:   WLQRQDY
- ProtClustDB:   PRK00022
- BioCyc:   PFLU205922:PFL_4751-MONOMER
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 22728; Mature: 22728

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLRHVIVFSFIALLAGCAGFGARESVEGHGNPAQWAANKQHLTGLDGWQIDGKIGIRAP
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCEEEEECC
KDSGSGTLFWLQRQDYYDIRLSGPLGRGAARLTGRPGAVSLEVANQGRYEAPTPEALVEE
CCCCCEEEEEEECCCEEEEEECCCCCCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHH
QLGWKLPVSHLAWWVRGLPAPDSKSRLTLDGNSRLANLDQDGWQVEYLSYAEQNGYWLPE
HCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCEEECCCCCCCEEEEEEEECCCCEECCC
RIKLHGSDLDVTLVIKTWQPRKLGQ
EEEEECCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MFLRHVIVFSFIALLAGCAGFGARESVEGHGNPAQWAANKQHLTGLDGWQIDGKIGIRAP
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCEEEEECC
KDSGSGTLFWLQRQDYYDIRLSGPLGRGAARLTGRPGAVSLEVANQGRYEAPTPEALVEE
CCCCCEEEEEEECCCEEEEEECCCCCCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHH
QLGWKLPVSHLAWWVRGLPAPDSKSRLTLDGNSRLANLDQDGWQVEYLSYAEQNGYWLPE
HCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCEEECCCCCCCEEEEEEEECCCCEECCC
RIKLHGSDLDVTLVIKTWQPRKLGQ
EEEEECCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA