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The map label for this gene is prfA [H]

Identifier: 77460969

GI number: 77460969

Start: 5351132

End: 5352214

Strand: Reverse

Name: prfA [H]

Synonym: Pfl01_4748

Alternate gene names: 77460969

Gene position: 5352214-5351132 (Counterclockwise)

Preceding gene: 77460970

Following gene: 77460968

Centisome position: 83.13

GC content: 60.85

Gene sequence:

>1083_bases
ATGAAAGCGTCACTGCTCAATAAGCTGGACATTCTCCAGGACCGTTTCGAGGAACTGACCGCGCTGCTCGGCGACGGCGA
AGTCATTTCCGACCAGACCAAATTCCGCGCTTATTCCAAGGAATACGCCGAACTTGAGCCGGTGGTACAAGGCTATAAAA
AGCTGCTCGGCGTACAAGGCGATCTTGAAGGCGCACAGGCGCTGCTCAAGGACAGCGACCCGGACATGCGTGAAATGGCC
GTGGAAGAAGTCCGCGAAGCCAAGGAATTGCTGATCACGCTGGAATCCGACCTGCAACGCATGCTGCTGCCCAAGGATCC
CAACGACGGGCGCAACGTGTTTCTCGAAATCCGCGCCGGCACCGGTGGTGACGAGGCGGCGATCTTCTCGGGTGACCTTT
TCCGCATGTACTCGCGTTACGCCGAACGCCGTGGCTGGCGCGTGGAGATCCTCTCGGAAAACGAAGGCGAACACGGTGGC
TATAAAGAAGTCATCGCCCGCATCGAAGGCGACAACGTTTACGGCAAGCTGAAATTCGAATCCGGCGCGCACCGCGTACA
GCGTGTACCCGCCACCGAATCCCAGGGCCGTATCCATACCTCGGCCTGCACCGTGGCGGTATTGCCCGAGCCGGACGAGC
GTGAAGCCATCGAGATCAACCCGGCGGATTTGCGCGTCGACACTTACCGCTCCTCCGGGGCCGGTGGTCAGCACGTCAAC
ACCACCGATTCGGCGATCCGCATCACCCACATACCGACCGGCACCGTTGTCGAGTGCCAGGAAGAACGTTCCCAGCACAA
GAACCGTGCCCGGGCGATGGCCTGGCTGTCGGCCAAGCTCAACGACCAGCAGACCGCAGCGGCGGCCAACGCCATCGCCA
GCGAACGTAAATTGCTGGTGGGTTCCGGTGACCGCTCCGAGCGTATCCGCACCTATAACTTTGCCCAGGGACGGGTCACC
GACCATCGCGTCAACCTGACCCTGTATTCCCTCGACGAAATCCTCGCCGGTGGCGTCGAAGCGGTGATCGAGCCGTTGCT
GGCCGAATACCAGGCCGACCAGCTCGCAGCGATAGGTGAGTAA

Upstream 100 bases:

>100_bases
AAAAGCTTTCTGCCGAAGGCCGCCTCGATGCGCTGGCCATGGCTCAGGAACTCTTTGCCCTCGAGGGCTCACCGGATAGC
TTTTCGGATAAAAAACCGCA

Downstream 100 bases:

>100_bases
ATGACCATCATCGCCAGCCTGTTGCGCGCCGCCGAACTGCCGGACTCGCCCACCGCGCGTCTGGACGCCGAATTGCTTCT
GGCTGCCGCGCTGGGCAAAT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKASLLNKLDILQDRFEELTALLGDGEVISDQTKFRAYSKEYAELEPVVQGYKKLLGVQGDLEGAQALLKDSDPDMREMA
VEEVREAKELLITLESDLQRMLLPKDPNDGRNVFLEIRAGTGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGG
YKEVIARIEGDNVYGKLKFESGAHRVQRVPATESQGRIHTSACTVAVLPEPDEREAIEINPADLRVDTYRSSGAGGQHVN
TTDSAIRITHIPTGTVVECQEERSQHKNRARAMAWLSAKLNDQQTAAAANAIASERKLLVGSGDRSERIRTYNFAQGRVT
DHRVNLTLYSLDEILAGGVEAVIEPLLAEYQADQLAAIGE

Sequences:

>Translated_360_residues
MKASLLNKLDILQDRFEELTALLGDGEVISDQTKFRAYSKEYAELEPVVQGYKKLLGVQGDLEGAQALLKDSDPDMREMA
VEEVREAKELLITLESDLQRMLLPKDPNDGRNVFLEIRAGTGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGG
YKEVIARIEGDNVYGKLKFESGAHRVQRVPATESQGRIHTSACTVAVLPEPDEREAIEINPADLRVDTYRSSGAGGQHVN
TTDSAIRITHIPTGTVVECQEERSQHKNRARAMAWLSAKLNDQQTAAAANAIASERKLLVGSGDRSERIRTYNFAQGRVT
DHRVNLTLYSLDEILAGGVEAVIEPLLAEYQADQLAAIGE
>Mature_360_residues
MKASLLNKLDILQDRFEELTALLGDGEVISDQTKFRAYSKEYAELEPVVQGYKKLLGVQGDLEGAQALLKDSDPDMREMA
VEEVREAKELLITLESDLQRMLLPKDPNDGRNVFLEIRAGTGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGG
YKEVIARIEGDNVYGKLKFESGAHRVQRVPATESQGRIHTSACTVAVLPEPDEREAIEINPADLRVDTYRSSGAGGQHVN
TTDSAIRITHIPTGTVVECQEERSQHKNRARAMAWLSAKLNDQQTAAAANAIASERKLLVGSGDRSERIRTYNFAQGRVT
DHRVNLTLYSLDEILAGGVEAVIEPLLAEYQADQLAAIGE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=48.014440433213, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI34577120, Length=323, Percent_Identity=41.7956656346749, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=46.6321243523316, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=66.1111111111111, Blast_Score=480, Evalue=1e-137,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=40.1759530791789, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=299, Percent_Identity=37.7926421404682, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=43.0379746835443, Blast_Score=239, Evalue=7e-64,
Organism=Drosophila melanogaster, GI19921226, Length=328, Percent_Identity=39.6341463414634, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39885; Mature: 39885

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLLNKLDILQDRFEELTALLGDGEVISDQTKFRAYSKEYAELEPVVQGYKKLLGVQG
CCCHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLEGAQALLKDSDPDMREMAVEEVREAKELLITLESDLQRMLLPKDPNDGRNVFLEIRAG
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
TGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGGYKEVIARIEGDNVYGKLKFE
CCCCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHEECCCCEEEEEEEC
SGAHRVQRVPATESQGRIHTSACTVAVLPEPDEREAIEINPADLRVDTYRSSGAGGQHVN
CCCHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCC
TTDSAIRITHIPTGTVVECQEERSQHKNRARAMAWLSAKLNDQQTAAAANAIASERKLLV
CCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEE
GSGDRSERIRTYNFAQGRVTDHRVNLTLYSLDEILAGGVEAVIEPLLAEYQADQLAAIGE
ECCCCCCCEEEEECCCCCEECEEEEEEEEEHHHHHHCCHHHHHHHHHHHHCHHHHHHCCC
>Mature Secondary Structure
MKASLLNKLDILQDRFEELTALLGDGEVISDQTKFRAYSKEYAELEPVVQGYKKLLGVQG
CCCHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DLEGAQALLKDSDPDMREMAVEEVREAKELLITLESDLQRMLLPKDPNDGRNVFLEIRAG
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
TGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGGYKEVIARIEGDNVYGKLKFE
CCCCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHEECCCCEEEEEEEC
SGAHRVQRVPATESQGRIHTSACTVAVLPEPDEREAIEINPADLRVDTYRSSGAGGQHVN
CCCHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCC
TTDSAIRITHIPTGTVVECQEERSQHKNRARAMAWLSAKLNDQQTAAAANAIASERKLLV
CCCCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEE
GSGDRSERIRTYNFAQGRVTDHRVNLTLYSLDEILAGGVEAVIEPLLAEYQADQLAAIGE
ECCCCCCCEEEEECCCCCEECEEEEEEEEEHHHHHHCCHHHHHHHHHHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA