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The map label for this gene is murI [H]

Identifier: 77460966

GI number: 77460966

Start: 5348768

End: 5349559

Strand: Reverse

Name: murI [H]

Synonym: Pfl01_4745

Alternate gene names: 77460966

Gene position: 5349559-5348768 (Counterclockwise)

Preceding gene: 77460967

Following gene: 77460965

Centisome position: 83.09

GC content: 62.75

Gene sequence:

>792_bases
ATGCGTGAAGCGCCGATTGCCGTCTTCGATTCCGGCGTCGGCGGTCTGTCGGTGCTGGCGGAAATCCAACAACTGCTGCC
GAATGAATCGCTGCTGTACCTCGGCGATTGCGGCCATATTCCTTATGGCGAGAAAACCCCGGAATTCATCCGCCAGCGTT
GCAGCGTGATGGCCGGTTTTTTCCGCGAGCAGGGCGCCAAGGCGCTGGTGATCGCGTGCAACACCGCGACCGTGGCCGGC
GTCGCCGATCTGCGCCGGGATTTTCCCGAGTGGCCGATCGTCGGCATGGAGCCGGCGGTCAAACCGGCGGCCGCCGCCAC
CCGCAGCGGTGTTGTGGGCGTACTCGCCACTACCGGGACCTTGCAGAGCGCGAAATTTGCGGCGCTGCTCGATCGCTTCG
CAACGGATGTGCGGGTCATCACGCAGCCATGCCCTGGACTGGTGGAGCTGATTGAAAGCGGCGACCTGCACAGTCCCCAG
TTGCGTGCCTTGCTCAAGCACTATGTCGATCCGCTTCTGGCCAACGGTTGCGACACGATCATCCTCGGCTGCACCCACTA
CCCGTTTCTCAAGCCGATGCTCAAGTCGATGATCCCCGAGAGCATCAGCCTGATCGATACGGGCGCGGCCGTGGCTCGAC
AACTGCAGCGACTTCTGGCCGAGCGTGACCTGCTCGCCGAAGGGCCGAACCGCCCGGTCAGATTCTGGACCAGCGCCGAT
CCCGAGTCTTTGCGCAGTACCTTGGTCCTGCTGGGCCAAGTCGATGGCGACGTCGAAAAATTCACCTTGTAA

Upstream 100 bases:

>100_bases
CCACTGGTGGGTCGTCTGCTGTTGATCGATGCACTGGGCTCGCGTTTCCGTGAGCTGCGGGTCAAGCGCGACCCCGGTTG
CAGCGTCTGTGGTTCGCAGC

Downstream 100 bases:

>100_bases
AAAAAATGTGAAATATCTAAAAAGTCAGCTGAACTTCTGACCAAGCGCAGATTTCTATAGCGCAGAGTGACCTAGAAAAC
CAAACGATCGTTCCGGAAAA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MREAPIAVFDSGVGGLSVLAEIQQLLPNESLLYLGDCGHIPYGEKTPEFIRQRCSVMAGFFREQGAKALVIACNTATVAG
VADLRRDFPEWPIVGMEPAVKPAAAATRSGVVGVLATTGTLQSAKFAALLDRFATDVRVITQPCPGLVELIESGDLHSPQ
LRALLKHYVDPLLANGCDTIILGCTHYPFLKPMLKSMIPESISLIDTGAAVARQLQRLLAERDLLAEGPNRPVRFWTSAD
PESLRSTLVLLGQVDGDVEKFTL

Sequences:

>Translated_263_residues
MREAPIAVFDSGVGGLSVLAEIQQLLPNESLLYLGDCGHIPYGEKTPEFIRQRCSVMAGFFREQGAKALVIACNTATVAG
VADLRRDFPEWPIVGMEPAVKPAAAATRSGVVGVLATTGTLQSAKFAALLDRFATDVRVITQPCPGLVELIESGDLHSPQ
LRALLKHYVDPLLANGCDTIILGCTHYPFLKPMLKSMIPESISLIDTGAAVARQLQRLLAERDLLAEGPNRPVRFWTSAD
PESLRSTLVLLGQVDGDVEKFTL
>Mature_263_residues
MREAPIAVFDSGVGGLSVLAEIQQLLPNESLLYLGDCGHIPYGEKTPEFIRQRCSVMAGFFREQGAKALVIACNTATVAG
VADLRRDFPEWPIVGMEPAVKPAAAATRSGVVGVLATTGTLQSAKFAALLDRFATDVRVITQPCPGLVELIESGDLHSPQ
LRALLKHYVDPLLANGCDTIILGCTHYPFLKPMLKSMIPESISLIDTGAAVARQLQRLLAERDLLAEGPNRPVRFWTSAD
PESLRSTLVLLGQVDGDVEKFTL

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=241, Percent_Identity=37.7593360995851, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 28334; Mature: 28334

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREAPIAVFDSGVGGLSVLAEIQQLLPNESLLYLGDCGHIPYGEKTPEFIRQRCSVMAGF
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
FREQGAKALVIACNTATVAGVADLRRDFPEWPIVGMEPAVKPAAAATRSGVVGVLATTGT
HHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCC
LQSAKFAALLDRFATDVRVITQPCPGLVELIESGDLHSPQLRALLKHYVDPLLANGCDTI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEE
ILGCTHYPFLKPMLKSMIPESISLIDTGAAVARQLQRLLAERDLLAEGPNRPVRFWTSAD
EECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
PESLRSTLVLLGQVDGDVEKFTL
HHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MREAPIAVFDSGVGGLSVLAEIQQLLPNESLLYLGDCGHIPYGEKTPEFIRQRCSVMAGF
CCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
FREQGAKALVIACNTATVAGVADLRRDFPEWPIVGMEPAVKPAAAATRSGVVGVLATTGT
HHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCC
LQSAKFAALLDRFATDVRVITQPCPGLVELIESGDLHSPQLRALLKHYVDPLLANGCDTI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEE
ILGCTHYPFLKPMLKSMIPESISLIDTGAAVARQLQRLLAERDLLAEGPNRPVRFWTSAD
EECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
PESLRSTLVLLGQVDGDVEKFTL
HHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA