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The map label for this gene is hprA [H]

Identifier: 77460932

GI number: 77460932

Start: 5319634

End: 5320599

Strand: Direct

Name: hprA [H]

Synonym: Pfl01_4711

Alternate gene names: 77460932

Gene position: 5319634-5320599 (Clockwise)

Preceding gene: 77460926

Following gene: 77460933

Centisome position: 82.62

GC content: 65.63

Gene sequence:

>966_bases
ATGACGAACGCCCACCGCGCCGTATTCCTCGATCACCCGTCGCTGGATCTTGGCGATCTCGATCTGAGCCCTTTGCGTGA
CTGCTTCAGCGATCTGCAACTGTTCGCCCAGACGTTGCCTGCGCAGGTTGCCGAACGCCTGCAAGGTGCGACCGTGGCGA
TCAGCAACAAGGTCCTGATCGACGCCGCCGCCATGGCCGCCAACCCACAGCTGAAGCTGATCCTGATCACCGCCACCGGC
ACCAACAACGTCGATCTGGCCGCCGCCCGCGCCCACGGCATCACCGTGTGCAACTGTCAGGGTTACGGCACGCCGTCGGT
GGCGCAGCACACGATCATGCTGTTGCTCAACCTCGCCACGCGTCTGGCCGATTATCAAAAAGCCGTCAGCCAAGGCCGCT
GGCAGCAAGCGAAACAGTTCTGCCTGCTGGATTACCCGATCGTCGAGCTGGAAGGCAAAACCCTTGGCTTGCTCGGTCAT
GGCGAACTCGGCGGCGCGGTCGCGCGACTGGCCGAAGCCTTCGGCATGCGCGTGTTGCTGGGGCAGATTCCGGGGCGCCC
GGCCCGTCCGGATCGCCTGCCACTGGAAGAATTGCTGCCGCAAATCGACGCCCTCACCCTGCACTGCCCGCTCAACGAAC
ACACCCGGCACTTCATCGGCGCCCGCGAACTCGCCTCGATGAAACCCGGCGCGTTCGTGGTCAACACCGCCCGTGGCGGA
CTGATTGACGAACAGGCTCTGGCCGACGCCCTGCGCAGCGGTCATCTGGGCGGCGCGGCCACCGATGTGCTGAGCGTCGA
GCCGCCGACCAACGGCAATCCGCTGCTGGCCGCCGACATCCCGCGCCTGATCGTCACCCCGCACAACGCCTGGGGCAGCC
GCGAGGCGCGGCAGCGAATCGTCGGCCAACTGGTCGAAAACACCCAGGCGTTCTTCAGCGGTAAGGCGCTGCGGGTCGTC
AGTTGA

Upstream 100 bases:

>100_bases
CCAGTACATGGGGGCTCCTTTCGGCCAAGCGTAACGAGTTGTTTCATCTGGTAGGCTCGGCAGATTACGCCTTCCCTTCA
AAGCACAACAGGTACAGTTG

Downstream 100 bases:

>100_bases
TAAACTGCGGCACTTTTTTTCACAGGAGCAGTTATGGATCCGCGCAGTGAAGTACTGCTTCGTCAGGCCGAGTTATTCCA
GGGTTCGGTGTTGCTGGCCG

Product: glycerate dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTNAHRAVFLDHPSLDLGDLDLSPLRDCFSDLQLFAQTLPAQVAERLQGATVAISNKVLIDAAAMAANPQLKLILITATG
TNNVDLAAARAHGITVCNCQGYGTPSVAQHTIMLLLNLATRLADYQKAVSQGRWQQAKQFCLLDYPIVELEGKTLGLLGH
GELGGAVARLAEAFGMRVLLGQIPGRPARPDRLPLEELLPQIDALTLHCPLNEHTRHFIGARELASMKPGAFVVNTARGG
LIDEQALADALRSGHLGGAATDVLSVEPPTNGNPLLAADIPRLIVTPHNAWGSREARQRIVGQLVENTQAFFSGKALRVV
S

Sequences:

>Translated_321_residues
MTNAHRAVFLDHPSLDLGDLDLSPLRDCFSDLQLFAQTLPAQVAERLQGATVAISNKVLIDAAAMAANPQLKLILITATG
TNNVDLAAARAHGITVCNCQGYGTPSVAQHTIMLLLNLATRLADYQKAVSQGRWQQAKQFCLLDYPIVELEGKTLGLLGH
GELGGAVARLAEAFGMRVLLGQIPGRPARPDRLPLEELLPQIDALTLHCPLNEHTRHFIGARELASMKPGAFVVNTARGG
LIDEQALADALRSGHLGGAATDVLSVEPPTNGNPLLAADIPRLIVTPHNAWGSREARQRIVGQLVENTQAFFSGKALRVV
S
>Mature_320_residues
TNAHRAVFLDHPSLDLGDLDLSPLRDCFSDLQLFAQTLPAQVAERLQGATVAISNKVLIDAAAMAANPQLKLILITATGT
NNVDLAAARAHGITVCNCQGYGTPSVAQHTIMLLLNLATRLADYQKAVSQGRWQQAKQFCLLDYPIVELEGKTLGLLGHG
ELGGAVARLAEAFGMRVLLGQIPGRPARPDRLPLEELLPQIDALTLHCPLNEHTRHFIGARELASMKPGAFVVNTARGGL
IDEQALADALRSGHLGGAATDVLSVEPPTNGNPLLAADIPRLIVTPHNAWGSREARQRIVGQLVENTQAFFSGKALRVVS

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=238, Percent_Identity=40.3361344537815, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4557499, Length=238, Percent_Identity=40.3361344537815, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI61743967, Length=238, Percent_Identity=39.4957983193277, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI4557497, Length=238, Percent_Identity=39.4957983193277, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI145580575, Length=237, Percent_Identity=40.084388185654, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI23308577, Length=276, Percent_Identity=33.695652173913, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI6912396, Length=286, Percent_Identity=28.3216783216783, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789279, Length=232, Percent_Identity=36.2068965517241, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787645, Length=259, Percent_Identity=30.5019305019305, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI87082289, Length=262, Percent_Identity=29.0076335877863, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788660, Length=304, Percent_Identity=27.3026315789474, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081824, Length=238, Percent_Identity=26.890756302521, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=280, Percent_Identity=32.1428571428571, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI25147481, Length=288, Percent_Identity=33.6805555555556, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320925, Length=284, Percent_Identity=31.3380281690141, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=234, Percent_Identity=35.042735042735, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324055, Length=250, Percent_Identity=30.8, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=303, Percent_Identity=27.7227722772277, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324980, Length=173, Percent_Identity=29.4797687861272, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24646446, Length=254, Percent_Identity=39.3700787401575, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24646448, Length=254, Percent_Identity=39.3700787401575, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24646452, Length=254, Percent_Identity=39.3700787401575, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24646450, Length=254, Percent_Identity=39.3700787401575, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI62472511, Length=312, Percent_Identity=35.8974358974359, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI19921140, Length=271, Percent_Identity=33.5793357933579, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI28574286, Length=265, Percent_Identity=27.5471698113208, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571528, Length=332, Percent_Identity=27.1084337349398, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585516, Length=300, Percent_Identity=24, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28574284, Length=302, Percent_Identity=24.8344370860927, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585514, Length=302, Percent_Identity=24.8344370860927, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574282, Length=302, Percent_Identity=24.8344370860927, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45552429, Length=302, Percent_Identity=24.8344370860927, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45551003, Length=302, Percent_Identity=24.8344370860927, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34287; Mature: 34156

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAHRAVFLDHPSLDLGDLDLSPLRDCFSDLQLFAQTLPAQVAERLQGATVAISNKVLI
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEE
DAAAMAANPQLKLILITATGTNNVDLAAARAHGITVCNCQGYGTPSVAQHTIMLLLNLAT
EHHHHCCCCCEEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
RLADYQKAVSQGRWQQAKQFCLLDYPIVELEGKTLGLLGHGELGGAVARLAEAFGMRVLL
HHHHHHHHHHCCHHHHHHCEEEECCCEEEECCCEEEEEECCCHHHHHHHHHHHHCHHEEE
GQIPGRPARPDRLPLEELLPQIDALTLHCPLNEHTRHFIGARELASMKPGAFVVNTARGG
CCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCC
LIDEQALADALRSGHLGGAATDVLSVEPPTNGNPLLAADIPRLIVTPHNAWGSREARQRI
CCCHHHHHHHHHCCCCCCCHHCEEEECCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHH
VGQLVENTQAFFSGKALRVVS
HHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
TNAHRAVFLDHPSLDLGDLDLSPLRDCFSDLQLFAQTLPAQVAERLQGATVAISNKVLI
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEE
DAAAMAANPQLKLILITATGTNNVDLAAARAHGITVCNCQGYGTPSVAQHTIMLLLNLAT
EHHHHCCCCCEEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
RLADYQKAVSQGRWQQAKQFCLLDYPIVELEGKTLGLLGHGELGGAVARLAEAFGMRVLL
HHHHHHHHHHCCHHHHHHCEEEECCCEEEECCCEEEEEECCCHHHHHHHHHHHHCHHEEE
GQIPGRPARPDRLPLEELLPQIDALTLHCPLNEHTRHFIGARELASMKPGAFVVNTARGG
CCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCC
LIDEQALADALRSGHLGGAATDVLSVEPPTNGNPLLAADIPRLIVTPHNAWGSREARQRI
CCCHHHHHHHHHCCCCCCCHHCEEEECCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHH
VGQLVENTQAFFSGKALRVVS
HHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]