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The map label for this gene is murD [H]

Identifier: 77460896

GI number: 77460896

Start: 5277432

End: 5278778

Strand: Reverse

Name: murD [H]

Synonym: Pfl01_4675

Alternate gene names: 77460896

Gene position: 5278778-5277432 (Counterclockwise)

Preceding gene: 77460897

Following gene: 77460895

Centisome position: 81.99

GC content: 65.33

Gene sequence:

>1347_bases
GTGTCTCTGATCGCTTCTGACCACTTCCGCATCGTTGTCGGCCTCGGCAAGAGCGGCATGTCCCTGGTTCGCTTCCTGGC
GAACCGGGGCGTGGCGTTTGCCGTGGCCGATACGCGGGAAAATCCACCGGAACTGGCCACGCTCCAGCGTGACTATCCGC
ACGTGGAAGTGCGTTGTGGCGAGCTGGACGTCGAGTTCCTGTGCCGTGCCGACGAGCTCTACGTGAGCCCCGGCTTGGCG
CTGGCGACCCCGGCCCTGCAAGCCGCCGCGGCCCGTGGCGTGAAGCTGTCCGGCGACATCGAGCTGTTCGCGCGTAACGC
GAAGGCGCCGATCGTGGCCATCAGCGGTTCCAACGCGAAAAGCACCGTCACCACCTTGGTCGGCGAGATGGCGGCTGCGG
CCGGCAAGCGCGTGGCCGTGGGCGGCAACCTCGGTACGCCGGCGCTGGATCTGCTCAGCGATGACGTCGAGCTGTACGTG
ATGGAACTGTCGAGTTTCCAGCTGGAAACCACCGACCAGCTCAACGCCGAAGTGGCGACCGTGCTCAACATCAGCGAAGA
CCACATGGACCGCTACAGCGGTCTGCCGGCGTATCACCTGGCCAAACACCGGATCTTCCGTGGCGCCAAGCAGTTCGTGG
TCAACCGTCAGGATGCGTTGACCCGTCCGTTGATGGGCGAGGGCCAGCCGTGCTGGACCTTCGGCCTGAGCAAACCGGAT
TTCAAGGCGTTCGGCCTGCGTGAAGAGAATGGCGAGAAATACCTGGCCTTCGAATTCCAGAACCTGATGCCGGTGCGCGA
ACTGAAGATTCGCGGCGCCCACAACCAGTCCAACGCCCTGGCGGCGCTGGCACTCGGCCACGCGGTCGGGCTGCCGTTCG
ACGCCATGCTGGTGGCGCTGCGCACCTTCGCCGGTCTCGAACACCGTTGTCAGTGGGTGCGTGATCTGGACGGCGTCGGT
TACTACAACGATTCCAAAGCCACCAACGTGGGCGCCGCACTGGCCGCCATCGAAGGCCTGGGTGCCGACATCGACGGCAA
GGTCGTGCTGATCGCCGGTGGCGACGGCAAGGGCGCCGAATTCAAGGACCTGCGCGATCCGGTGGCGACCCATTGCCGCG
CCGTGATCCTGATGGGCCGTGACTCCGACAAGATCGGCGAAGCGATCGGCGATGCCGTTCCGCTGATTCGCGCCACCACG
CTGGTCGATGCGGTCGCCCAGTGCCGCGCCGCCGCCCAGCCGGGAGACGTGGTGCTGCTGTCGCCGGCCTGCGCCAGTTT
CGACATGTTCAATAACTACGAAGACCGTGGTCACCAGTTCGTCCGCGCCGTGGAGGATCTGGCATGA

Upstream 100 bases:

>100_bases
TCAAGGGCTGGCCCGAGCCGCGCGTGATCGTCCGTTTCTGGATCATCACCGTGATTCTCGTGCTGATCGGCCTTGCCACC
CTGAAGCTGAGGTAGAACGA

Downstream 100 bases:

>100_bases
GCTTGAGAAACATCATCAAGCCATACCCGTCGCCGCTCATTACCGGACGCGGAATCGACCTCGACTTCCCGATGCTCGCC
GGTTGCCTGGCGTTGCTCGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSLIASDHFRIVVGLGKSGMSLVRFLANRGVAFAVADTRENPPELATLQRDYPHVEVRCGELDVEFLCRADELYVSPGLA
LATPALQAAAARGVKLSGDIELFARNAKAPIVAISGSNAKSTVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDDVELYV
MELSSFQLETTDQLNAEVATVLNISEDHMDRYSGLPAYHLAKHRIFRGAKQFVVNRQDALTRPLMGEGQPCWTFGLSKPD
FKAFGLREENGEKYLAFEFQNLMPVRELKIRGAHNQSNALAALALGHAVGLPFDAMLVALRTFAGLEHRCQWVRDLDGVG
YYNDSKATNVGAALAAIEGLGADIDGKVVLIAGGDGKGAEFKDLRDPVATHCRAVILMGRDSDKIGEAIGDAVPLIRATT
LVDAVAQCRAAAQPGDVVLLSPACASFDMFNNYEDRGHQFVRAVEDLA

Sequences:

>Translated_448_residues
MSLIASDHFRIVVGLGKSGMSLVRFLANRGVAFAVADTRENPPELATLQRDYPHVEVRCGELDVEFLCRADELYVSPGLA
LATPALQAAAARGVKLSGDIELFARNAKAPIVAISGSNAKSTVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDDVELYV
MELSSFQLETTDQLNAEVATVLNISEDHMDRYSGLPAYHLAKHRIFRGAKQFVVNRQDALTRPLMGEGQPCWTFGLSKPD
FKAFGLREENGEKYLAFEFQNLMPVRELKIRGAHNQSNALAALALGHAVGLPFDAMLVALRTFAGLEHRCQWVRDLDGVG
YYNDSKATNVGAALAAIEGLGADIDGKVVLIAGGDGKGAEFKDLRDPVATHCRAVILMGRDSDKIGEAIGDAVPLIRATT
LVDAVAQCRAAAQPGDVVLLSPACASFDMFNNYEDRGHQFVRAVEDLA
>Mature_447_residues
SLIASDHFRIVVGLGKSGMSLVRFLANRGVAFAVADTRENPPELATLQRDYPHVEVRCGELDVEFLCRADELYVSPGLAL
ATPALQAAAARGVKLSGDIELFARNAKAPIVAISGSNAKSTVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDDVELYVM
ELSSFQLETTDQLNAEVATVLNISEDHMDRYSGLPAYHLAKHRIFRGAKQFVVNRQDALTRPLMGEGQPCWTFGLSKPDF
KAFGLREENGEKYLAFEFQNLMPVRELKIRGAHNQSNALAALALGHAVGLPFDAMLVALRTFAGLEHRCQWVRDLDGVGY
YNDSKATNVGAALAAIEGLGADIDGKVVLIAGGDGKGAEFKDLRDPVATHCRAVILMGRDSDKIGEAIGDAVPLIRATTL
VDAVAQCRAAAQPGDVVLLSPACASFDMFNNYEDRGHQFVRAVEDLA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=443, Percent_Identity=49.4356659142212, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47950; Mature: 47819

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIASDHFRIVVGLGKSGMSLVRFLANRGVAFAVADTRENPPELATLQRDYPHVEVRCG
CCCCCCCCEEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHCCCCEEEEEEC
ELDVEFLCRADELYVSPGLALATPALQAAAARGVKLSGDIELFARNAKAPIVAISGSNAK
CCCEEHHCCCCCEEECCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCEEEEECCCCH
STVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDDVELYVMELSSFQLETTDQLNAEVAT
HHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCEEEEEEECCCEECCCHHCCCEEEE
VLNISEDHMDRYSGLPAYHLAKHRIFRGAKQFVVNRQDALTRPLMGEGQPCWTFGLSKPD
EEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCC
FKAFGLREENGEKYLAFEFQNLMPVRELKIRGAHNQSNALAALALGHAVGLPFDAMLVAL
CCEECCCCCCCCEEEEEECCCCCCHHHHEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHH
RTFAGLEHRCQWVRDLDGVGYYNDSKATNVGAALAAIEGLGADIDGKVVLIAGGDGKGAE
HHHHCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
FKDLRDPVATHCRAVILMGRDSDKIGEAIGDAVPLIRATTLVDAVAQCRAAAQPGDVVLL
HHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
SPACASFDMFNNYEDRGHQFVRAVEDLA
CCCCHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLIASDHFRIVVGLGKSGMSLVRFLANRGVAFAVADTRENPPELATLQRDYPHVEVRCG
CCCCCCCEEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHCCCCEEEEEEC
ELDVEFLCRADELYVSPGLALATPALQAAAARGVKLSGDIELFARNAKAPIVAISGSNAK
CCCEEHHCCCCCEEECCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCEEEEECCCCH
STVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDDVELYVMELSSFQLETTDQLNAEVAT
HHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCCEEEEEEECCCEECCCHHCCCEEEE
VLNISEDHMDRYSGLPAYHLAKHRIFRGAKQFVVNRQDALTRPLMGEGQPCWTFGLSKPD
EEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCC
FKAFGLREENGEKYLAFEFQNLMPVRELKIRGAHNQSNALAALALGHAVGLPFDAMLVAL
CCEECCCCCCCCEEEEEECCCCCCHHHHEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHH
RTFAGLEHRCQWVRDLDGVGYYNDSKATNVGAALAAIEGLGADIDGKVVLIAGGDGKGAE
HHHHCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
FKDLRDPVATHCRAVILMGRDSDKIGEAIGDAVPLIRATTLVDAVAQCRAAAQPGDVVLL
HHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
SPACASFDMFNNYEDRGHQFVRAVEDLA
CCCCHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA