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The map label for this gene is hmp [H]

Identifier: 77460873

GI number: 77460873

Start: 5249399

End: 5250580

Strand: Reverse

Name: hmp [H]

Synonym: Pfl01_4652

Alternate gene names: 77460873

Gene position: 5250580-5249399 (Counterclockwise)

Preceding gene: 77460879

Following gene: 77460872

Centisome position: 81.55

GC content: 61.59

Gene sequence:

>1182_bases
ATGCTTAGCGTCCAGGATCGTGCCATCGTCAAATCCACTGTGCCTCTGCTGGAAAGCGGCGGTGAAGCGCTGATCACCCA
TTTCTACCGGATGATGCTCTCCGAGTACCCGGAAGTCCGCCCGCTGTTCAACCAGGCCCACCAGGCCAGCGGTGACCAGC
CTCGCGCCCTGGCCAACGGCGTGTTGATGTACGCCCGTCACATCGATCAACTCGACCAGTTGGGCGATCTGGTGGCCAAG
ATCATCAACAAGCACGTGGCGTTGCAGATTCTGCCGGAACACTACCCGATCGTCGGCACCTGCCTGCTGCGCGCCATCTC
CGAAGTGCTCGGCGAAGAAATCGCCACGCCGGAGGTGATGAGCGCCTGGGGCGCGGCTTACGGTCAGTTGGCCGAGATTC
TGATCGGTGCCGAAACCGCCATTTATGACCAGAAAGAGCAAGCCGTCGGCGGCTGGCGCGGGGCACGGGAGTTCATCGTC
GCGGCCAAGGTCGAGGAGAGCGCGGAAATCATTTCGTTCTACTTTGAACCGGCGGACAAAGGCCCGATCCTCGCAGCCGA
ACCTGGCCAGTACATCGGCATGAAGCTGGTGCTCGACGGTGAGGAAATCCGCCGCAACTATTCGCTGTCGGCCCTGGCCA
ACAAAGGCCAATACCGCATCAGCGTCAAACGCGAACCGGGCGGCCGTGCCTCCAATTACCTGCATGATCAACTGCATGTC
GGCGCCAGTATTCAGCTGTTCCCGCCATCGGGTGAGTTCACCCTGACTGCCAGCGACAAACCGCTGGTGCTGATCAGCGG
CGGCGTCGGCATCACTCCGACACTGGCGATGCTGGAAGCGGCGCTGGAAACCGAGCGTCCGGTGTACTTCATCCACAGTG
CGCGCAATGGCAGCGTGCATGCGTTCCGCGACTGGATCGACGGGCTGGCCGCGCGTCATCCGCAGCTCAAGCGCTTCTAT
TGCTACGCCGAAGATGATGGTGTGAGCCCGGCGGCGGACAAGGTCGGGTTGTTGAGCGAGGAGCAACTGGGCGAGTGGCT
GCCGGCGGAGCGTGATGTGGACGCTTACTTCCTTGGGCCGAAAGGTTTCATGGGGGCGATCAAGCGTCATCTGAAGGCGC
TGGGTGTGCCTGAGAAGCAGAGCCGGTATGAGTTCTTCGGACCGGCTGCTGCTTTGGAATGA

Upstream 100 bases:

>100_bases
ACTACATAATTTTCAAGCTATTGATTTATATAGAAAAAATAGTTGGCACGAATACTGGAATGGATAAGGCACTTTTAGAG
AAGGCTCAGGAGTCACCCTT

Downstream 100 bases:

>100_bases
TCTGAAACCGGGTTGCCTTCATCGCGGGCTTGCCCGCGATGGGGGCATCGCTAACTTGATTCTTAAATTCTGTTTAAAGG
TTAATCGTTTCTTAACCGGT

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLSVQDRAIVKSTVPLLESGGEALITHFYRMMLSEYPEVRPLFNQAHQASGDQPRALANGVLMYARHIDQLDQLGDLVAK
IINKHVALQILPEHYPIVGTCLLRAISEVLGEEIATPEVMSAWGAAYGQLAEILIGAETAIYDQKEQAVGGWRGAREFIV
AAKVEESAEIISFYFEPADKGPILAAEPGQYIGMKLVLDGEEIRRNYSLSALANKGQYRISVKREPGGRASNYLHDQLHV
GASIQLFPPSGEFTLTASDKPLVLISGGVGITPTLAMLEAALETERPVYFIHSARNGSVHAFRDWIDGLAARHPQLKRFY
CYAEDDGVSPAADKVGLLSEEQLGEWLPAERDVDAYFLGPKGFMGAIKRHLKALGVPEKQSRYEFFGPAAALE

Sequences:

>Translated_393_residues
MLSVQDRAIVKSTVPLLESGGEALITHFYRMMLSEYPEVRPLFNQAHQASGDQPRALANGVLMYARHIDQLDQLGDLVAK
IINKHVALQILPEHYPIVGTCLLRAISEVLGEEIATPEVMSAWGAAYGQLAEILIGAETAIYDQKEQAVGGWRGAREFIV
AAKVEESAEIISFYFEPADKGPILAAEPGQYIGMKLVLDGEEIRRNYSLSALANKGQYRISVKREPGGRASNYLHDQLHV
GASIQLFPPSGEFTLTASDKPLVLISGGVGITPTLAMLEAALETERPVYFIHSARNGSVHAFRDWIDGLAARHPQLKRFY
CYAEDDGVSPAADKVGLLSEEQLGEWLPAERDVDAYFLGPKGFMGAIKRHLKALGVPEKQSRYEFFGPAAALE
>Mature_393_residues
MLSVQDRAIVKSTVPLLESGGEALITHFYRMMLSEYPEVRPLFNQAHQASGDQPRALANGVLMYARHIDQLDQLGDLVAK
IINKHVALQILPEHYPIVGTCLLRAISEVLGEEIATPEVMSAWGAAYGQLAEILIGAETAIYDQKEQAVGGWRGAREFIV
AAKVEESAEIISFYFEPADKGPILAAEPGQYIGMKLVLDGEEIRRNYSLSALANKGQYRISVKREPGGRASNYLHDQLHV
GASIQLFPPSGEFTLTASDKPLVLISGGVGITPTLAMLEAALETERPVYFIHSARNGSVHAFRDWIDGLAARHPQLKRFY
CYAEDDGVSPAADKVGLLSEEQLGEWLPAERDVDAYFLGPKGFMGAIKRHLKALGVPEKQSRYEFFGPAAALE

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=401, Percent_Identity=40.3990024937656, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1787658, Length=241, Percent_Identity=29.4605809128631, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788104, Length=223, Percent_Identity=23.3183856502242, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=409, Percent_Identity=39.119804400978, Blast_Score=232, Evalue=7e-62,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 43086; Mature: 43086

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVQDRAIVKSTVPLLESGGEALITHFYRMMLSEYPEVRPLFNQAHQASGDQPRALANG
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHH
VLMYARHIDQLDQLGDLVAKIINKHVALQILPEHYPIVGTCLLRAISEVLGEEIATPEVM
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
SAWGAAYGQLAEILIGAETAIYDQKEQAVGGWRGAREFIVAAKVEESAEIISFYFEPADK
HHHHHHHHHHHHHHHCCHHHHHCCHHHHHCCHHHHHHHEEEEHHHHHHEEEEEEECCCCC
GPILAAEPGQYIGMKLVLDGEEIRRNYSLSALANKGQYRISVKREPGGRASNYLHDQLHV
CCEEEECCCCEEEEEEEECHHHHHHCCCHHHHCCCCEEEEEEEECCCCCCHHHHHHHHCC
GASIQLFPPSGEFTLTASDKPLVLISGGVGITPTLAMLEAALETERPVYFIHSARNGSVH
CCEEEEECCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHH
AFRDWIDGLAARHPQLKRFYCYAEDDGVSPAADKVGLLSEEQLGEWLPAERDVDAYFLGP
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHCCCCHHHHHHCCCCCCCCCEEEECC
KGFMGAIKRHLKALGVPEKQSRYEFFGPAAALE
HHHHHHHHHHHHHCCCCCHHCCCHHCCCHHCCC
>Mature Secondary Structure
MLSVQDRAIVKSTVPLLESGGEALITHFYRMMLSEYPEVRPLFNQAHQASGDQPRALANG
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHH
VLMYARHIDQLDQLGDLVAKIINKHVALQILPEHYPIVGTCLLRAISEVLGEEIATPEVM
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
SAWGAAYGQLAEILIGAETAIYDQKEQAVGGWRGAREFIVAAKVEESAEIISFYFEPADK
HHHHHHHHHHHHHHHCCHHHHHCCHHHHHCCHHHHHHHEEEEHHHHHHEEEEEEECCCCC
GPILAAEPGQYIGMKLVLDGEEIRRNYSLSALANKGQYRISVKREPGGRASNYLHDQLHV
CCEEEECCCCEEEEEEEECHHHHHHCCCHHHHCCCCEEEEEEEECCCCCCHHHHHHHHCC
GASIQLFPPSGEFTLTASDKPLVLISGGVGITPTLAMLEAALETERPVYFIHSARNGSVH
CCEEEEECCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHH
AFRDWIDGLAARHPQLKRFYCYAEDDGVSPAADKVGLLSEEQLGEWLPAERDVDAYFLGP
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHCCCCHHHHHHCCCCCCCCCEEEECC
KGFMGAIKRHLKALGVPEKQSRYEFFGPAAALE
HHHHHHHHHHHHHCCCCCHHCCCHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]