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The map label for this gene is yefM [H]

Identifier: 77460855

GI number: 77460855

Start: 5234185

End: 5234508

Strand: Direct

Name: yefM [H]

Synonym: Pfl01_4634

Alternate gene names: 77460855

Gene position: 5234185-5234508 (Clockwise)

Preceding gene: 77460854

Following gene: 77460856

Centisome position: 81.3

GC content: 50.31

Gene sequence:

>324_bases
ATGATAGGTATCGCCTTGTCATCCTGTACTCATTTCTGTACGTTCTTTGCATCAAGCGAGGACTACAACATGGACACTAT
CAACTACACCACAGCTCGTGCGCACTTGGCTGAAACCATGGATCGCGTGAATGAAGACTGCGCCCCGCTTCTGGTAACCC
GACAAAAAGGCGAGCCTGTAGTGATGATGTCTCTGGCCGAATACAACGCGCTGGAAGAAACGGCTTATCTGCTGCGTTCT
CCGGCCAATGCCGAGCGCTTGATCAAATCAATTGGCGAAATGCGCGCTGGAAAAGCCAAAGTCAGGCAACTGATTGAAGA
ATGA

Upstream 100 bases:

>100_bases
GCAGGCGCGTCGGAATCAGCTTACAAAAGACCCAGAAACGTCTCGACGAGACTTCGGTGATACTCAGCACACCGCAGGCA
GCCCGTCTGTGGCACAACCA

Downstream 100 bases:

>100_bases
AAATCCAGTTCACGCCAACGGGCTGGGAAGACTACTTATGGTTTCAACAAAACGATAAGGCCGGTCTCAAACGAATCAAT
CTTTTGATCAAAGCGATCCA

Product: prevent-host-death protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 107; Mature: 107

Protein sequence:

>107_residues
MIGIALSSCTHFCTFFASSEDYNMDTINYTTARAHLAETMDRVNEDCAPLLVTRQKGEPVVMMSLAEYNALEETAYLLRS
PANAERLIKSIGEMRAGKAKVRQLIEE

Sequences:

>Translated_107_residues
MIGIALSSCTHFCTFFASSEDYNMDTINYTTARAHLAETMDRVNEDCAPLLVTRQKGEPVVMMSLAEYNALEETAYLLRS
PANAERLIKSIGEMRAGKAKVRQLIEE
>Mature_107_residues
MIGIALSSCTHFCTFFASSEDYNMDTINYTTARAHLAETMDRVNEDCAPLLVTRQKGEPVVMMSLAEYNALEETAYLLRS
PANAERLIKSIGEMRAGKAKVRQLIEE

Specific function: Part of a toxin-antitoxin (TA) module. Antitoxin that counteracts the effect of the YoeB mRNA interferase. YefM binds to the promoter region of the yefM-yeoB operon to repress transcription, YeoB acts as a corepressor [H]

COG id: COG2161

COG function: function code D; Antitoxin of toxin-antitoxin stability system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YefM antitoxin family [H]

Homologues:

Organism=Escherichia coli, GI87082026, Length=83, Percent_Identity=57.8313253012048, Blast_Score=99, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006442 [H]

Pfam domain/function: PF02604 PhdYeFM [H]

EC number: NA

Molecular weight: Translated: 11965; Mature: 11965

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGIALSSCTHFCTFFASSEDYNMDTINYTTARAHLAETMDRVNEDCAPLLVTRQKGEPV
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCE
VMMSLAEYNALEETAYLLRSPANAERLIKSIGEMRAGKAKVRQLIEE
EEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHC
>Mature Secondary Structure
MIGIALSSCTHFCTFFASSEDYNMDTINYTTARAHLAETMDRVNEDCAPLLVTRQKGEPV
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCE
VMMSLAEYNALEETAYLLRSPANAERLIKSIGEMRAGKAKVRQLIEE
EEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]