Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 77460825

GI number: 77460825

Start: 5205549

End: 5206697

Strand: Reverse

Name: rlmN [H]

Synonym: Pfl01_4604

Alternate gene names: 77460825

Gene position: 5206697-5205549 (Counterclockwise)

Preceding gene: 77460826

Following gene: 77460824

Centisome position: 80.87

GC content: 58.14

Gene sequence:

>1149_bases
ATGACGACATCGACTGTTAAAAACAACCTGCTGGGTCTGACCCAGCCGGAAATGGAAAAATTCTTCGACTCAATCGGGGA
GAAGCGTTTCCGTGCCGGTCAGGTAATGAAATGGATTCACCACTTTGGCGTCGATGATTTCGACGCCATGACGAACGTCA
GCAAGGCCTTGCGCGAAAAGCTCAAGGCTGTTGCTGAGGTCCGTGGTCCCGAAGTGGTCAGCGAGGACATTTCCAGCGAC
GGCACCCGCAAATGGGTGGTGCGCGTGGCGTCCGGCAGCTGCGTCGAGACCGTTTACATTCCCCAGGGCAAACGCGGCAC
TCTGTGCGTTTCGTCCCAGGCAGGCTGTGCCCTGGATTGCAGTTTCTGCTCCACCGGCAAGCAAGGCTTCAACAGCAACC
TCACCGCCGCCGAAGTCATCGGCCAGGTGTGGATTGCCAACAAATCCTTCGGCAGTGTTCCGGCTACCATCGACCGCGCC
ATCACCAACGTGGTGATGATGGGCATGGGCGAACCGCTGCTGAACTTCGACAACGTCGTGGCCGCCATGCATCTGATGAT
GGATGACCTGGGCTACGGGATCTCCAAGCGCCGCGTGACCCTGTCCACGTCGGGTGTGGTGCCGATGATCGATGAGCTGG
CCAAGCACATCGACGTATCCCTGGCGTTGTCCCTGCACGCACCGAATGACGCATTGCGTAACCAATTGGTGCCGATCAAC
AAGAAATATCCGCTTAAGATGCTGCTCGAGTCGTGCCAGCGCTACATGTCCGCCCTTGGCGAGAAACGTGTGCTGACCAT
CGAGTACACCTTGCTCAAGGACGTCAACGACAAGCTTGAGCACGCGGTGGAAATGATCGAGCTGCTCAAGGATATTCCGT
GCAAGATCAACCTGATTCCGTTCAACCCGTTCCCGCATTCCGGGTACGAGCGGCCGAGCAACAACGCCATCCGCCGGTTC
CAGGATCAGCTTCACCAGGCCGGTTTCAACGTCACCGTGCGCACCACCCGTGGTGAAGACATCGACGCGGCTTGTGGTCA
ATTGGTAGGGCAGGTGCTGGATCGCACCCGTCGCAGCGAACGTTACATCGCCGTCCGCGAATTGAGCGCCGACAGCGATC
TGGCACAGAACGCCGCGAACACTAACTAA

Upstream 100 bases:

>100_bases
TCGGACTCCGAAGCCGCTGCTGCTCGCGAAATCGCTTACTTCTTCGCAGCTACTGAAGTAACCACTCGCTAAGCATTGGC
TTAAGAGTGAAGGTGAATCC

Downstream 100 bases:

>100_bases
GAGAGGATCTCTATGTCCCTGCGCTTTGCGCTGCTGTTGCTGTTGGCCAGCCTGTGTGCTGGTTGTGTCCTGTCGGGCGA
CTACAACCCGATGAAGACCA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTTSTVKNNLLGLTQPEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREKLKAVAEVRGPEVVSEDISSD
GTRKWVVRVASGSCVETVYIPQGKRGTLCVSSQAGCALDCSFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATIDRA
ITNVVMMGMGEPLLNFDNVVAAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELAKHIDVSLALSLHAPNDALRNQLVPIN
KKYPLKMLLESCQRYMSALGEKRVLTIEYTLLKDVNDKLEHAVEMIELLKDIPCKINLIPFNPFPHSGYERPSNNAIRRF
QDQLHQAGFNVTVRTTRGEDIDAACGQLVGQVLDRTRRSERYIAVRELSADSDLAQNAANTN

Sequences:

>Translated_382_residues
MTTSTVKNNLLGLTQPEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREKLKAVAEVRGPEVVSEDISSD
GTRKWVVRVASGSCVETVYIPQGKRGTLCVSSQAGCALDCSFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATIDRA
ITNVVMMGMGEPLLNFDNVVAAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELAKHIDVSLALSLHAPNDALRNQLVPIN
KKYPLKMLLESCQRYMSALGEKRVLTIEYTLLKDVNDKLEHAVEMIELLKDIPCKINLIPFNPFPHSGYERPSNNAIRRF
QDQLHQAGFNVTVRTTRGEDIDAACGQLVGQVLDRTRRSERYIAVRELSADSDLAQNAANTN
>Mature_381_residues
TTSTVKNNLLGLTQPEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREKLKAVAEVRGPEVVSEDISSDG
TRKWVVRVASGSCVETVYIPQGKRGTLCVSSQAGCALDCSFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATIDRAI
TNVVMMGMGEPLLNFDNVVAAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELAKHIDVSLALSLHAPNDALRNQLVPINK
KYPLKMLLESCQRYMSALGEKRVLTIEYTLLKDVNDKLEHAVEMIELLKDIPCKINLIPFNPFPHSGYERPSNNAIRRFQ
DQLHQAGFNVTVRTTRGEDIDAACGQLVGQVLDRTRRSERYIAVRELSADSDLAQNAANTN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=363, Percent_Identity=59.7796143250689, Blast_Score=442, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42211; Mature: 42080

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSTVKNNLLGLTQPEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREK
CCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKAVAEVRGPEVVSEDISSDGTRKWVVRVASGSCVETVYIPQGKRGTLCVSSQAGCALDC
HHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCEEEC
SFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATIDRAITNVVMMGMGEPLLNFDNVV
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
AAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELAKHIDVSLALSLHAPNDALRNQLVPIN
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHHHHCCCCCCC
KKYPLKMLLESCQRYMSALGEKRVLTIEYTLLKDVNDKLEHAVEMIELLKDIPCKINLIP
CCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
FNPFPHSGYERPSNNAIRRFQDQLHQAGFNVTVRTTRGEDIDAACGQLVGQVLDRTRRSE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
RYIAVRELSADSDLAQNAANTN
CEEEEEECCCCHHHHHHCCCCC
>Mature Secondary Structure 
TTSTVKNNLLGLTQPEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREK
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LKAVAEVRGPEVVSEDISSDGTRKWVVRVASGSCVETVYIPQGKRGTLCVSSQAGCALDC
HHHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCEEEC
SFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATIDRAITNVVMMGMGEPLLNFDNVV
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
AAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELAKHIDVSLALSLHAPNDALRNQLVPIN
HHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCHHHHCCCCCCC
KKYPLKMLLESCQRYMSALGEKRVLTIEYTLLKDVNDKLEHAVEMIELLKDIPCKINLIP
CCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
FNPFPHSGYERPSNNAIRRFQDQLHQAGFNVTVRTTRGEDIDAACGQLVGQVLDRTRRSE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
RYIAVRELSADSDLAQNAANTN
CEEEEEECCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA