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The map label for this gene is groEL [H]

Identifier: 77460722

GI number: 77460722

Start: 5076862

End: 5078508

Strand: Reverse

Name: groEL [H]

Synonym: Pfl01_4501

Alternate gene names: 77460722

Gene position: 5078508-5076862 (Counterclockwise)

Preceding gene: 77460723

Following gene: 77460719

Centisome position: 78.88

GC content: 58.41

Gene sequence:

>1647_bases
ATGGCTGCTAAAGAAGTTAAATTCGGCGATTCCGCCCGCAAGAAAATGCTGGTTGGCGTAAACGTCCTGGCTGACGCGGT
AAAAGCGACCCTGGGCCCGAAAGGCCGTAACGTGATCCTCGAGAAGAGCTTCGGCGCTCCGACCATCACCAAGGACGGCG
TTTCCGTTGCCAAGGAAATCGAGCTGGAAGACCGTTTCGAAAACATGGGCGCGCAGCTGGTCAAAGACGTTGCCTCCCGT
GCCAACGATGACGCTGGTGACGGTACTACCACCGCTACCGTTCTGGCTCAGTCGATCGTCAACGAAGGCCTGAAAGCCGT
CGCTGCCGGCATGAACCCGATGGACCTGAAGCGCGGTATCGACAAAGCGACCATCGCTGTCGTTGCCGAGCTGAAAAAAC
TGTCCGCACCATGCACCGACACTAAAGCGATCGCTCAGGTTGGCACCATCTCCGCCAACTCCGACAACTCCATCGGCGAC
ATCATTGCCGAAGCCATGGAAAAAGTCGGTAAAGAAGGCGTGATCACCGTTGAAGAAGGCTCTGGCCTGGAAAACGAACT
GTCGGTTGTAGAAGGCATGCAGTTCGACCGTGGCTACCTGTCCCCATACTTCGTCAACAAGCCGGACACCATGGTCGCCG
AGCTGGAAGGCGCGCTGATCCTGCTGGTCGACAAGAAGATCTCGAACATCCGCGAAATGCTGCCAGTACTGGAAGCCGTT
GCCAAAGCCGGCCGTCCACTGCTGATCGTTGCCGAAGACGTTGAAGGCGAAGCCCTGGCGACTCTGGTAGTGAACAACAT
GCGTGGCATCGTTAAAGTCGCAGCCGTCAAGGCTCCAGGCTTCGGCGACCGTCGCAAGGCCATGCTGCAGGACATCGCCG
TTCTGACCGGTGGTACCGTTATCTCCGAAGAGATCGGCCTGAGCCTGGAAAGCACCACCCTGGAACACCTGGGTAGCGCC
AAGCGCGTGACCCTGTCCAAGGAAAACACCATCATCGTTGACGGTGCTGGCAATGACGGCGACATCCAGGCGCGTATCGC
TCAGATCCGTGCCCAGGTTGCCGAGACTTCCTCGGACTACGACCGTGAAAAACTGCAAGAGCGTCTGGCCAAGCTGTCCG
GCGGCGTTGCAGTGATCAAGGTTGGCGCCGGTTCCGAAGTTGAAATGAAAGAGAAGAAAGCCCGCGTTGAAGACGCCCTA
CACGCAACCCGCGCAGCCGTTGAAGAAGGCGTGGTACCTGGCGGTGGCGTTGCGCTGATCCGCGCTCTGAACGCGATCAT
CAACCTCAAAGGCGACAACGCCGACCAGGACGTAGGTATCGCTGTTCTGCGTCGTGCCGTTGAAGCACCGCTGCGTCAGA
TCGCTGCCAACAGCGGTGACGAGCCAAGCGTTGTGGTCAACGAAGTCAAGAACGGCAAAGGTAACTTCGGTTACAACGCT
GCGACTGGCGAATACGGCGACATGATCGAAATGGGCATCCTGGACCCAACCAAGGTAACCCGTTCCGCTCTGCAAGCTGC
ATCGTCGATCGGCGGTCTGATCCTGACCACCGAAGCTGCTGTTGCTGACGCACCGAAGAAAGACGGTGCTGCTGGCGGCG
GTATGCCAGACATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAA

Upstream 100 bases:

>100_bases
CCTGCTGGTAATGAGCGAGAACGAAATCCTCGCCGTTATCGAAGGCTGATTACCCCCGTTCATTTTCCCGCTACTACAAA
GTATTTAAGGAATATCGATC

Downstream 100 bases:

>100_bases
GCCAGCCTTACCCAGTAACGAAAAACCCCGTCTGCGTGAGCAGACGGGGTTTTTTATTGCCCGGGTTTCAGGGTTCAGGC
GTTGGCGGGTTGTGCCAGCG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAAKEVKFGDSARKKMLVGVNVLADAVKATLGPKGRNVILEKSFGAPTITKDGVSVAKEIELEDRFENMGAQLVKDVASR
ANDDAGDGTTTATVLAQSIVNEGLKAVAAGMNPMDLKRGIDKATIAVVAELKKLSAPCTDTKAIAQVGTISANSDNSIGD
IIAEAMEKVGKEGVITVEEGSGLENELSVVEGMQFDRGYLSPYFVNKPDTMVAELEGALILLVDKKISNIREMLPVLEAV
AKAGRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAVLTGGTVISEEIGLSLESTTLEHLGSA
KRVTLSKENTIIVDGAGNDGDIQARIAQIRAQVAETSSDYDREKLQERLAKLSGGVAVIKVGAGSEVEMKEKKARVEDAL
HATRAAVEEGVVPGGGVALIRALNAIINLKGDNADQDVGIAVLRRAVEAPLRQIAANSGDEPSVVVNEVKNGKGNFGYNA
ATGEYGDMIEMGILDPTKVTRSALQAASSIGGLILTTEAAVADAPKKDGAAGGGMPDMGGMGGMGGMM

Sequences:

>Translated_548_residues
MAAKEVKFGDSARKKMLVGVNVLADAVKATLGPKGRNVILEKSFGAPTITKDGVSVAKEIELEDRFENMGAQLVKDVASR
ANDDAGDGTTTATVLAQSIVNEGLKAVAAGMNPMDLKRGIDKATIAVVAELKKLSAPCTDTKAIAQVGTISANSDNSIGD
IIAEAMEKVGKEGVITVEEGSGLENELSVVEGMQFDRGYLSPYFVNKPDTMVAELEGALILLVDKKISNIREMLPVLEAV
AKAGRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAVLTGGTVISEEIGLSLESTTLEHLGSA
KRVTLSKENTIIVDGAGNDGDIQARIAQIRAQVAETSSDYDREKLQERLAKLSGGVAVIKVGAGSEVEMKEKKARVEDAL
HATRAAVEEGVVPGGGVALIRALNAIINLKGDNADQDVGIAVLRRAVEAPLRQIAANSGDEPSVVVNEVKNGKGNFGYNA
ATGEYGDMIEMGILDPTKVTRSALQAASSIGGLILTTEAAVADAPKKDGAAGGGMPDMGGMGGMGGMM
>Mature_547_residues
AAKEVKFGDSARKKMLVGVNVLADAVKATLGPKGRNVILEKSFGAPTITKDGVSVAKEIELEDRFENMGAQLVKDVASRA
NDDAGDGTTTATVLAQSIVNEGLKAVAAGMNPMDLKRGIDKATIAVVAELKKLSAPCTDTKAIAQVGTISANSDNSIGDI
IAEAMEKVGKEGVITVEEGSGLENELSVVEGMQFDRGYLSPYFVNKPDTMVAELEGALILLVDKKISNIREMLPVLEAVA
KAGRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAVLTGGTVISEEIGLSLESTTLEHLGSAK
RVTLSKENTIIVDGAGNDGDIQARIAQIRAQVAETSSDYDREKLQERLAKLSGGVAVIKVGAGSEVEMKEKKARVEDALH
ATRAAVEEGVVPGGGVALIRALNAIINLKGDNADQDVGIAVLRRAVEAPLRQIAANSGDEPSVVVNEVKNGKGNFGYNAA
TGEYGDMIEMGILDPTKVTRSALQAASSIGGLILTTEAAVADAPKKDGAAGGGMPDMGGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=51.2333965844402, Blast_Score=531, Evalue=1e-151,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=51.2333965844402, Blast_Score=531, Evalue=1e-151,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=77.7358490566038, Blast_Score=825, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=52.0833333333333, Blast_Score=555, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI193210679, Length=210, Percent_Identity=50.9523809523809, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI17564182, Length=557, Percent_Identity=22.4416517055655, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=54.5627376425855, Blast_Score=556, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322524, Length=162, Percent_Identity=29.6296296296296, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=51.6129032258064, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=51.6129032258064, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=50.381679389313, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=50.381679389313, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=50.381679389313, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI17864606, Length=553, Percent_Identity=43.5804701627486, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24584129, Length=528, Percent_Identity=38.6363636363636, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI19921262, Length=528, Percent_Identity=38.6363636363636, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 56839; Mature: 56708

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKEVKFGDSARKKMLVGVNVLADAVKATLGPKGRNVILEKSFGAPTITKDGVSVAKEI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
ELEDRFENMGAQLVKDVASRANDDAGDGTTTATVLAQSIVNEGLKAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
DKATIAVVAELKKLSAPCTDTKAIAQVGTISANSDNSIGDIIAEAMEKVGKEGVITVEEG
CHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
SGLENELSVVEGMQFDRGYLSPYFVNKPDTMVAELEGALILLVDKKISNIREMLPVLEAV
CCCHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
AKAGRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAVLTGGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCEE
ISEEIGLSLESTTLEHLGSAKRVTLSKENTIIVDGAGNDGDIQARIAQIRAQVAETSSDY
EHHHHCCEEHHHHHHHCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
DREKLQERLAKLSGGVAVIKVGAGSEVEMKEKKARVEDALHATRAAVEEGVVPGGGVALI
HHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RALNAIINLKGDNADQDVGIAVLRRAVEAPLRQIAANSGDEPSVVVNEVKNGKGNFGYNA
HHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCC
ATGEYGDMIEMGILDPTKVTRSALQAASSIGGLILTTEAAVADAPKKDGAAGGGMPDMGG
CCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHCCCCCCCCCCCCCCCCCC
MGGMGGMM
CCCCCCCC
>Mature Secondary Structure 
AAKEVKFGDSARKKMLVGVNVLADAVKATLGPKGRNVILEKSFGAPTITKDGVSVAKEI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
ELEDRFENMGAQLVKDVASRANDDAGDGTTTATVLAQSIVNEGLKAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
DKATIAVVAELKKLSAPCTDTKAIAQVGTISANSDNSIGDIIAEAMEKVGKEGVITVEEG
CHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECC
SGLENELSVVEGMQFDRGYLSPYFVNKPDTMVAELEGALILLVDKKISNIREMLPVLEAV
CCCHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
AKAGRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAMLQDIAVLTGGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCEE
ISEEIGLSLESTTLEHLGSAKRVTLSKENTIIVDGAGNDGDIQARIAQIRAQVAETSSDY
EHHHHCCEEHHHHHHHCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
DREKLQERLAKLSGGVAVIKVGAGSEVEMKEKKARVEDALHATRAAVEEGVVPGGGVALI
HHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RALNAIINLKGDNADQDVGIAVLRRAVEAPLRQIAANSGDEPSVVVNEVKNGKGNFGYNA
HHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCC
ATGEYGDMIEMGILDPTKVTRSALQAASSIGGLILTTEAAVADAPKKDGAAGGGMPDMGG
CCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHCCCCCCCCCCCCCCCCCC
MGGMGGMM
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA