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The map label for this gene is ilvI [H]

Identifier: 77460692

GI number: 77460692

Start: 5042494

End: 5044131

Strand: Reverse

Name: ilvI [H]

Synonym: Pfl01_4471

Alternate gene names: 77460692

Gene position: 5044131-5042494 (Counterclockwise)

Preceding gene: 77460694

Following gene: 77460691

Centisome position: 78.34

GC content: 65.63

Gene sequence:

>1638_bases
ATGGCGACGTGTGGCGAAGTACTGGTCAAGTTACTCGAAGATTATGGGGTCGAGCAGGTGTTCGGCATTCCCGGTGTGCA
TACCGTGGAGCTGTATCGCGGGCTGGCCCGTTCGAGCATCAACCACGTCACCCCGCGCCACGAGCAGGGCGCCGGCTTCA
TGGCCGACGGCTACGCCCGTACCAGCGGCAAACCAGGTGTGTGCTTCATCATCACCGGCCCTGGCATGACCAACATCACC
ACCGCCATGGGCCAGGCGTACGCCGATTCGATCCCGATGCTGGTGATCTCCAGTGTGCAGTCGCGCAGCCAATTGGGCGG
CGGTCGCGGCAAGCTGCATGAACTGCCGAACCAGAGCGCACTGGTCGGTGGCGTGGCGGCGTTCTCCCACACCCTGATGT
CGGCGTCCGAATTGCCGGGTGTGCTGGCCCGTGCGTTTGCGCTGTTCCAGGCCGGGCGTCCGCGTCCGGTGCACATCGAG
ATTCCGCTGGACGTGCTGGTCGAAGAAGCCGACGAACTGCTGACCAGCGTGCCGGTCAACATCGACCGCGCCGGAGCTGC
GCCGGCCGCCGTGAGCCGCATGACCGAACTGCTGGCCGGCGCCCGGCGTCCGCTGATCCTTGCCGGTGGCGGCGCCATCG
ATGCCGCTGCCGAACTCACCGAACTGGCTGAGCTGCTTGATGCACCAATGGCTCTGACCATCAACGCCAAAGGCATGCTG
CCGTCCAGCCATCCGCTGTTGATCGGTTCGACCCAGAGCCTGGTGGCGACCCGTGCTCTGGTGGCCGATGCCGACGTGAT
CCTGGCGATCGGCACCGAGCTGGCGGAAACCGACTACGACGTGACCTTCGCCGGCGGTTTCGAAATCCCCGGCAAGCTGC
TGCGTGTGGACATCGATCCCGACCAGACCGTGCGCAACTACCCGCCTCACGTGGCGCTGGTGGCCGACTCGCGCAATGCC
GCACAAGCGTTGCTCAGCGCGCTGTCGCACAAACCGCTGGCCGAGCGCCGCAACGATTGGGGCCAGGTACGCGCCGCCCG
TCTGCGTGAAGACCTGGCCGCGACCTGGGACGCGCCGACCCTGGCCCAGACCCGTTTCCTCGAAACCGTGCTGCACGAAT
TGCCGAACGCAGTGTTCGTCGGTGACTCCACGCAACCGGTGTACACCGGCAACCTGACGTTCAACCCGGAGCGTCCGCGC
CGCTGGTTCAACTCGTCCACCGGTTACGGCACCCTCGGTTACGCCTTGCCGGCGGCGATTGGCGCGTGGCTTGGCGGCAA
CCTCGAACACGGCGCGCGTCCGCCGGTGGTGTGCCTGATCGGCGACGGCGGTCTGCAATTCACCCTGCCGGAACTGGCCA
GCGCTGTGGAGGCGCGCACGCCGGTGATCATCCTGCTGTGGAATAACCAGGGTTACGAAGAGATCAAGAAATACATGGTC
AACCGTGCCATCGAGCCAGTGGGCGTCGACATCTACACCCCGGACTTCATCGGCGTGGCCAAGGCCCTGGGTTGCGCGGC
GCAAGCGATCAGCTCCGCTGAACATCTGCGCGGTGCACTGCGCGCCGCCACCGATCGTCAGGGCCCGACCCTGATTGAAA
TCGACCAGACCCAATGGATGAAGGCGGTGGCCCAATGA

Upstream 100 bases:

>100_bases
GCTTTTATCGATTGTTGCGGCTACGGGTCGCCCGTTTCAATGAGGCCAATCACCGGTTCCTTTACAGCCCGGTGCCTCAA
CGAACAAGAAGGGCAAAGAC

Downstream 100 bases:

>100_bases
TGAATTTCCCGACCACACTCGACGGCCTGTACATCAACGGCCAATGGTCCGCCGGCCGCGAACACCTGCGCGTCATCAAT
CCGGCCACCGAAGCGCTGTT

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MATCGEVLVKLLEDYGVEQVFGIPGVHTVELYRGLARSSINHVTPRHEQGAGFMADGYARTSGKPGVCFIITGPGMTNIT
TAMGQAYADSIPMLVISSVQSRSQLGGGRGKLHELPNQSALVGGVAAFSHTLMSASELPGVLARAFALFQAGRPRPVHIE
IPLDVLVEEADELLTSVPVNIDRAGAAPAAVSRMTELLAGARRPLILAGGGAIDAAAELTELAELLDAPMALTINAKGML
PSSHPLLIGSTQSLVATRALVADADVILAIGTELAETDYDVTFAGGFEIPGKLLRVDIDPDQTVRNYPPHVALVADSRNA
AQALLSALSHKPLAERRNDWGQVRAARLREDLAATWDAPTLAQTRFLETVLHELPNAVFVGDSTQPVYTGNLTFNPERPR
RWFNSSTGYGTLGYALPAAIGAWLGGNLEHGARPPVVCLIGDGGLQFTLPELASAVEARTPVIILLWNNQGYEEIKKYMV
NRAIEPVGVDIYTPDFIGVAKALGCAAQAISSAEHLRGALRAATDRQGPTLIEIDQTQWMKAVAQ

Sequences:

>Translated_545_residues
MATCGEVLVKLLEDYGVEQVFGIPGVHTVELYRGLARSSINHVTPRHEQGAGFMADGYARTSGKPGVCFIITGPGMTNIT
TAMGQAYADSIPMLVISSVQSRSQLGGGRGKLHELPNQSALVGGVAAFSHTLMSASELPGVLARAFALFQAGRPRPVHIE
IPLDVLVEEADELLTSVPVNIDRAGAAPAAVSRMTELLAGARRPLILAGGGAIDAAAELTELAELLDAPMALTINAKGML
PSSHPLLIGSTQSLVATRALVADADVILAIGTELAETDYDVTFAGGFEIPGKLLRVDIDPDQTVRNYPPHVALVADSRNA
AQALLSALSHKPLAERRNDWGQVRAARLREDLAATWDAPTLAQTRFLETVLHELPNAVFVGDSTQPVYTGNLTFNPERPR
RWFNSSTGYGTLGYALPAAIGAWLGGNLEHGARPPVVCLIGDGGLQFTLPELASAVEARTPVIILLWNNQGYEEIKKYMV
NRAIEPVGVDIYTPDFIGVAKALGCAAQAISSAEHLRGALRAATDRQGPTLIEIDQTQWMKAVAQ
>Mature_544_residues
ATCGEVLVKLLEDYGVEQVFGIPGVHTVELYRGLARSSINHVTPRHEQGAGFMADGYARTSGKPGVCFIITGPGMTNITT
AMGQAYADSIPMLVISSVQSRSQLGGGRGKLHELPNQSALVGGVAAFSHTLMSASELPGVLARAFALFQAGRPRPVHIEI
PLDVLVEEADELLTSVPVNIDRAGAAPAAVSRMTELLAGARRPLILAGGGAIDAAAELTELAELLDAPMALTINAKGMLP
SSHPLLIGSTQSLVATRALVADADVILAIGTELAETDYDVTFAGGFEIPGKLLRVDIDPDQTVRNYPPHVALVADSRNAA
QALLSALSHKPLAERRNDWGQVRAARLREDLAATWDAPTLAQTRFLETVLHELPNAVFVGDSTQPVYTGNLTFNPERPRR
WFNSSTGYGTLGYALPAAIGAWLGGNLEHGARPPVVCLIGDGGLQFTLPELASAVEARTPVIILLWNNQGYEEIKKYMVN
RAIEPVGVDIYTPDFIGVAKALGCAAQAISSAEHLRGALRAATDRQGPTLIEIDQTQWMKAVAQ

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=25.8467023172906, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI21361361, Length=551, Percent_Identity=28.3121597096189, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=30.2325581395349, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790104, Length=542, Percent_Identity=31.3653136531365, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=29.8932384341637, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788716, Length=549, Percent_Identity=26.2295081967213, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=23.4432234432234, Blast_Score=109, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=581, Percent_Identity=24.9569707401033, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=558, Percent_Identity=22.9390681003584, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17531301, Length=558, Percent_Identity=22.9390681003584, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=581, Percent_Identity=27.710843373494, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6320816, Length=497, Percent_Identity=23.5412474849095, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323163, Length=491, Percent_Identity=24.6435845213849, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=501, Percent_Identity=24.1516966067864, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=567, Percent_Identity=26.2786596119929, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 57826; Mature: 57695

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATCGEVLVKLLEDYGVEQVFGIPGVHTVELYRGLARSSINHVTPRHEQGAGFMADGYAR
CCCHHHHHHHHHHHCCCHHECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCC
TSGKPGVCFIITGPGMTNITTAMGQAYADSIPMLVISSVQSRSQLGGGRGKLHELPNQSA
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCH
LVGGVAAFSHTLMSASELPGVLARAFALFQAGRPRPVHIEIPLDVLVEEADELLTSVPVN
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCC
IDRAGAAPAAVSRMTELLAGARRPLILAGGGAIDAAAELTELAELLDAPMALTINAKGML
CCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
PSSHPLLIGSTQSLVATRALVADADVILAIGTELAETDYDVTFAGGFEIPGKLLRVDIDP
CCCCCEEEECHHHHHHHHHHHHCCCEEEEECCHHHCCCCCEEEECCCCCCCEEEEEECCC
DQTVRNYPPHVALVADSRNAAQALLSALSHKPLAERRNDWGQVRAARLREDLAATWDAPT
CCHHHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCH
LAQTRFLETVLHELPNAVFVGDSTQPVYTGNLTFNPERPRRWFNSSTGYGTLGYALPAAI
HHHHHHHHHHHHHCCCEEEECCCCCCEEECCEEECCCCHHHHHCCCCCCCHHHHHHHHHH
GAWLGGNLEHGARPPVVCLIGDGGLQFTLPELASAVEARTPVIILLWNNQGYEEIKKYMV
HHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
NRAIEPVGVDIYTPDFIGVAKALGCAAQAISSAEHLRGALRAATDRQGPTLIEIDQTQWM
HCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHH
KAVAQ
HHHCC
>Mature Secondary Structure 
ATCGEVLVKLLEDYGVEQVFGIPGVHTVELYRGLARSSINHVTPRHEQGAGFMADGYAR
CCHHHHHHHHHHHCCCHHECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCC
TSGKPGVCFIITGPGMTNITTAMGQAYADSIPMLVISSVQSRSQLGGGRGKLHELPNQSA
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCH
LVGGVAAFSHTLMSASELPGVLARAFALFQAGRPRPVHIEIPLDVLVEEADELLTSVPVN
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCC
IDRAGAAPAAVSRMTELLAGARRPLILAGGGAIDAAAELTELAELLDAPMALTINAKGML
CCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
PSSHPLLIGSTQSLVATRALVADADVILAIGTELAETDYDVTFAGGFEIPGKLLRVDIDP
CCCCCEEEECHHHHHHHHHHHHCCCEEEEECCHHHCCCCCEEEECCCCCCCEEEEEECCC
DQTVRNYPPHVALVADSRNAAQALLSALSHKPLAERRNDWGQVRAARLREDLAATWDAPT
CCHHHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCH
LAQTRFLETVLHELPNAVFVGDSTQPVYTGNLTFNPERPRRWFNSSTGYGTLGYALPAAI
HHHHHHHHHHHHHCCCEEEECCCCCCEEECCEEECCCCHHHHHCCCCCCCHHHHHHHHHH
GAWLGGNLEHGARPPVVCLIGDGGLQFTLPELASAVEARTPVIILLWNNQGYEEIKKYMV
HHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
NRAIEPVGVDIYTPDFIGVAKALGCAAQAISSAEHLRGALRAATDRQGPTLIEIDQTQWM
HCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHH
KAVAQ
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10555290 [H]