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The map label for this gene is fumA [H]

Identifier: 77460673

GI number: 77460673

Start: 5013534

End: 5015057

Strand: Direct

Name: fumA [H]

Synonym: Pfl01_4452

Alternate gene names: 77460673

Gene position: 5013534-5015057 (Clockwise)

Preceding gene: 77460669

Following gene: 77460674

Centisome position: 77.87

GC content: 62.99

Gene sequence:

>1524_bases
ATGACCGTGATCAAGCAAGACGACCTGATTCAGAGCGTTGCCGACGCCCTGCAGTTCATTTCCTACTACCACCCCGTGGA
CTTCATCCAGGCGATGCACGAAGCCTACCTGCGCGAAGAATCGCCAGCGGCCCGTGACTCGATGGCGCAGATCCTGATCA
ACTCGCGCATGTGCGCCACGGGCCACCGGCCGATCTGCCAGGACACCGGCATCGTGACCGTGTTCGTGCGTGTGGGCATG
GACGTGCGTTGGGATGGCGCGACCATGAGCCTGGACGACATGATCAACGAAGGCGTGCGCCGCGCCTACAACCTGCCGGA
AAACGTCCTGCGTGCCTCGATCCTCGCCGACCCGGCGGGCGCTCGTAAAAACACCAAGGACAACACCCCGGCGGTCATCC
ACTACTCCATCGTTCCGGGTAACACCGTGGAAGTGGACGTAGCGGCCAAGGGCGGCGGTTCCGAGAACAAGTCGAAGATG
GCCATGCTCAACCCGTCCGACTCGATCGTCGACTGGGTACTCAAGACCGTTCCGACCATGGGCGCCGGCTGGTGCCCGCC
GGGCATGCTGGGCATCGGCATCGGCGGTACCGCCGAGAAAGCCGCTGTCATGGCCAAGGAAGTGTTGATGGAATCCATCG
ACATTCACGAGCTCAAGGCCCGTGGCCCGCAGAACCGCATCGAAGAAATGCGTCTGGAGCTGTTCGAGAAGGTCAACCAG
CTGGGCATCGGCGCCCAGGGCCTCGGTGGCCTGACCACCGTCCTCGACGTGAAGATCATGGACTACCCGACCCACGCCGC
TTCCTTGCCGGTGTGCATGATCCCGAACTGCGCCGCCACCCGTCACGCGCACTTCGTATTGGACGGCTCCGGCCCGGCCT
CGCTGGAAGCGCCACCGCTGGACGCCTACCCGGAAATCGTCTGGGAAGCCGGCCCGTCGGCCCGTCGCGTCAACCTCGAC
ACCCTGACCCCGGAAGACGTGCAGAGCTGGAAGCCGGGCGAAACCGTCCTGCTCAATGGCAAGATGCTCACCGGTCGCGA
CGCTGCGCACAAACGCATGGTCGAGATGCTGAACAAGGGTGAAACCCTGCCGGTTGATCTGAAGGGCCGCTTCATCTACT
ACGTCGGCCCGGTTGATCCGGTCGGTGACGAAGTGGTTGGCCCGGCGGGTCCGACCACCGCCACGCGGATGGACAAGTTC
ACCCGTCAGATCCTTGAGCAGACCGGCCTGTTGGGCATGATCGGCAAGTCCGAGCGCGGCCCGACCGCCATCGACGCGAT
CAAGGACAACAAGGCTGTGTACCTGATGGCCGTCGGCGGTGCCGCTTACCTGGTGGCGCAAGCGATCAAGAAGTCCAAGG
TTCTGGCGTTCTCCGAACTGGGCATGGAAGCGATCTACGAGTTCGAGGTCAAGGACATGCCGGTCACCGTTGCGGTGGAC
AGCAAAGGTGAATCGGTGCACATCACCGGTCCTGCGATCTGGCAACAGAAGATCAGCGAAAGCCTGGCGGTGGAAGTGCA
GTAA

Upstream 100 bases:

>100_bases
AGCAGCCCCGGCGTGCCTTGAAAGGTGTCTGCGACCGACCGATGCACCCAAGCCGCAGGCACCTTATTGAATGTTCCCGT
CTTTTAGAGGAGCGCGACTC

Downstream 100 bases:

>100_bases
GCACTTCATCTGCTTGTAAAAAACCGGGCGGCCAGCGATGACCGCCCGGTTTTTTTTCGCCTGCCGTCAGTCGGCACCTG
TCAGATCTGACAGGTGCCGC

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSMAQILINSRMCATGHRPICQDTGIVTVFVRVGM
DVRWDGATMSLDDMINEGVRRAYNLPENVLRASILADPAGARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKM
AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQ
LGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPPLDAYPEIVWEAGPSARRVNLD
TLTPEDVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNKGETLPVDLKGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKF
TRQILEQTGLLGMIGKSERGPTAIDAIKDNKAVYLMAVGGAAYLVAQAIKKSKVLAFSELGMEAIYEFEVKDMPVTVAVD
SKGESVHITGPAIWQQKISESLAVEVQ

Sequences:

>Translated_507_residues
MTVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSMAQILINSRMCATGHRPICQDTGIVTVFVRVGM
DVRWDGATMSLDDMINEGVRRAYNLPENVLRASILADPAGARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKM
AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQ
LGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPPLDAYPEIVWEAGPSARRVNLD
TLTPEDVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNKGETLPVDLKGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKF
TRQILEQTGLLGMIGKSERGPTAIDAIKDNKAVYLMAVGGAAYLVAQAIKKSKVLAFSELGMEAIYEFEVKDMPVTVAVD
SKGESVHITGPAIWQQKISESLAVEVQ
>Mature_506_residues
TVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSMAQILINSRMCATGHRPICQDTGIVTVFVRVGMD
VRWDGATMSLDDMINEGVRRAYNLPENVLRASILADPAGARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKMA
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQL
GIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPPLDAYPEIVWEAGPSARRVNLDT
LTPEDVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNKGETLPVDLKGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFT
RQILEQTGLLGMIGKSERGPTAIDAIKDNKAVYLMAVGGAAYLVAQAIKKSKVLAFSELGMEAIYEFEVKDMPVTVAVDS
KGESVHITGPAIWQQKISESLAVEVQ

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=473, Percent_Identity=33.615221987315, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1790564, Length=471, Percent_Identity=32.9087048832272, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1789443, Length=167, Percent_Identity=34.1317365269461, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789442, Length=286, Percent_Identity=28.6713286713287, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54748; Mature: 54617

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSMAQILINSRMCAT
CCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECC
GHRPICQDTGIVTVFVRVGMDVRWDGATMSLDDMINEGVRRAYNLPENVLRASILADPAG
CCCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
ARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKMAMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQ
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPPL
HCCCCCCCCCEEEEEEEEEEECCCCHHCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCC
DAYPEIVWEAGPSARRVNLDTLTPEDVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNKG
CCCCHHHCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHCC
ETLPVDLKGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRQILEQTGLLGMIGKSERG
CCEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCC
PTAIDAIKDNKAVYLMAVGGAAYLVAQAIKKSKVLAFSELGMEAIYEFEVKDMPVTVAVD
CCHHHEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCEEEEEC
SKGESVHITGPAIWQQKISESLAVEVQ
CCCCEEEEECHHHHHHHHHHHHEEEEC
>Mature Secondary Structure 
TVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSMAQILINSRMCAT
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEECC
GHRPICQDTGIVTVFVRVGMDVRWDGATMSLDDMINEGVRRAYNLPENVLRASILADPAG
CCCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
ARKNTKDNTPAVIHYSIVPGNTVEVDVAAKGGGSENKSKMAMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAAVMAKEVLMESIDIHELKARGPQNRIEEMRLELFEKVNQ
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLDGSGPASLEAPPL
HCCCCCCCCCEEEEEEEEEEECCCCHHCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCC
DAYPEIVWEAGPSARRVNLDTLTPEDVQSWKPGETVLLNGKMLTGRDAAHKRMVEMLNKG
CCCCHHHCCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHCC
ETLPVDLKGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRQILEQTGLLGMIGKSERG
CCEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCC
PTAIDAIKDNKAVYLMAVGGAAYLVAQAIKKSKVLAFSELGMEAIYEFEVKDMPVTVAVD
CCHHHEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCEEEEEC
SKGESVHITGPAIWQQKISESLAVEVQ
CCCCEEEEECHHHHHHHHHHHHEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]