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The map label for this gene is pykA [H]

Identifier: 77460669

GI number: 77460669

Start: 5008455

End: 5009906

Strand: Direct

Name: pykA [H]

Synonym: Pfl01_4448

Alternate gene names: 77460669

Gene position: 5008455-5009906 (Clockwise)

Preceding gene: 77460666

Following gene: 77460673

Centisome position: 77.79

GC content: 63.77

Gene sequence:

>1452_bases
ATGTCCGTCCGTCGTACCAAAATCGTCGCTACCCTTGGCCCGGCCAGTAACTCGCCGGAAGTTCTCGAACAGCTGATTCT
GGCTGGCCTGGACGTCGCCCGTCTGAACTTCTCCCACGGCACCCCCGACGAGCACAAGGCTCGCGCGAAGCTGGTGCGTG
ACCTCGCGGCCAAGCACGGTCGCTTCGTCGCCCTGCTGGGTGACCTGCAAGGCCCGAAAATCCGTATCGCCAAATTCGCC
AACAAGCGCATCGAGCTGAAGATCGGTGACCAGTTCACCTTCTCCACCAGCCATCCGCTGACCGAAGGCAACCAGCAAGT
GGTCGGCATCGACTACCCGGACCTGGTCAAGGACTGCGGCGTGGGCGACGAGCTCCTGCTCGATGACGGCCGCGTGGTCA
TGCGCGTTGAAACCGCCACCGCCACCGAGCTGAACTGCGTCGTGACCATCGGCGGTCCGCTGTCCGACCACAAAGGCATC
AACCGTCGCGGTGGCGGCCTGACCGCGCCGGCCCTGACTGAAAAAGACAAGGCCGACATCAAGCTCGCCGCTGAAATGGA
AGTCGACTACCTCGCCGTGTCCTTCCCGCGTGACGCCGCCGACATGAACTACGCCCGTCAACTGCGCGACGAAGCCGGCG
GCACCGCCTGGCTGGTGGCGAAGATCGAACGCGCCGAAGCCGTGGCCGACGACGAAACCCTCGACGGTCTGATCAAGGCT
TCCGACGCGGTGATGGTTGCCCGTGGTGACCTCGGTGTGGAAATCGGCGACGCCGAGCTGGTGGGCATCCAGAAGAAAAT
CATTCTGCACGCACGCCGCCACAACAAGGCGGTGATCGTGGCGACCCAGATGATGGAGTCGATGATCCAGAACCCGATGC
CGACCCGCGCCGAAGTGTCCGACGTGGCCAACGCCGTGCTCGACTACACCGACGCCGTGATGCTGTCTGCCGAGTCCGCT
GCCGGCGCTTACCCGCTGGAAGCCGTGCAGGCGATGGCGCGCATCTGTGTCGGCGCTGAAAAGCACCCGACCAGTAAGAC
CTCCAGCCACCGTATCGGCAAGGAATTCGAGCGCTGCGACGAAAGCATCGCGCTGGCGACCATGTACACCGCCAACCACT
TCCCGGGCGTCAAGGCGATCATCGCACTGACCGAAAGCGGCTACACCCCGCTGATCATGTCGCGTATCCGCTCCTCGGTG
CCGATCTACTGCTTCACCCCGCACCGAGAAGCCCAGGCTCGCGCGGCAATGTTCCGTGGCGTGTACACCGTACCGTTCGA
CCCGGCTTCGCTGGCACCGAACGAAGTCAGCCAGAAAGCCATCGACGAACTGGTCAAGCGCGGCGTAGTGGAGAAAGGCG
ACTGGGTCATCCTGACCAAGGGCGACAGCTACCACACCACCGGCGGCACCAACGGCATGAAGATCCTGCACGTGGGCGAT
CCGCAGGTCTGA

Upstream 100 bases:

>100_bases
CGTGTTCAATAAAGAAAGTATTAAGTAGTGCAAACGAACAATGACTACACCCTTCGAGCATAGTAGCCTTCGCTGGCGCT
TGAACCTGAGGAGTCTTTGC

Downstream 100 bases:

>100_bases
GTGACCGGCTGCTGAAAACGAAAAGCCCCGCCATTTGAATGGCGGGGCTTTTTCATTTCAGGAGTGTTGATCGTTACTAC
GTTGATCGTTCCCACGCTCT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSVRRTKIVATLGPASNSPEVLEQLILAGLDVARLNFSHGTPDEHKARAKLVRDLAAKHGRFVALLGDLQGPKIRIAKFA
NKRIELKIGDQFTFSTSHPLTEGNQQVVGIDYPDLVKDCGVGDELLLDDGRVVMRVETATATELNCVVTIGGPLSDHKGI
NRRGGGLTAPALTEKDKADIKLAAEMEVDYLAVSFPRDAADMNYARQLRDEAGGTAWLVAKIERAEAVADDETLDGLIKA
SDAVMVARGDLGVEIGDAELVGIQKKIILHARRHNKAVIVATQMMESMIQNPMPTRAEVSDVANAVLDYTDAVMLSAESA
AGAYPLEAVQAMARICVGAEKHPTSKTSSHRIGKEFERCDESIALATMYTANHFPGVKAIIALTESGYTPLIMSRIRSSV
PIYCFTPHREAQARAAMFRGVYTVPFDPASLAPNEVSQKAIDELVKRGVVEKGDWVILTKGDSYHTTGGTNGMKILHVGD
PQV

Sequences:

>Translated_483_residues
MSVRRTKIVATLGPASNSPEVLEQLILAGLDVARLNFSHGTPDEHKARAKLVRDLAAKHGRFVALLGDLQGPKIRIAKFA
NKRIELKIGDQFTFSTSHPLTEGNQQVVGIDYPDLVKDCGVGDELLLDDGRVVMRVETATATELNCVVTIGGPLSDHKGI
NRRGGGLTAPALTEKDKADIKLAAEMEVDYLAVSFPRDAADMNYARQLRDEAGGTAWLVAKIERAEAVADDETLDGLIKA
SDAVMVARGDLGVEIGDAELVGIQKKIILHARRHNKAVIVATQMMESMIQNPMPTRAEVSDVANAVLDYTDAVMLSAESA
AGAYPLEAVQAMARICVGAEKHPTSKTSSHRIGKEFERCDESIALATMYTANHFPGVKAIIALTESGYTPLIMSRIRSSV
PIYCFTPHREAQARAAMFRGVYTVPFDPASLAPNEVSQKAIDELVKRGVVEKGDWVILTKGDSYHTTGGTNGMKILHVGD
PQV
>Mature_482_residues
SVRRTKIVATLGPASNSPEVLEQLILAGLDVARLNFSHGTPDEHKARAKLVRDLAAKHGRFVALLGDLQGPKIRIAKFAN
KRIELKIGDQFTFSTSHPLTEGNQQVVGIDYPDLVKDCGVGDELLLDDGRVVMRVETATATELNCVVTIGGPLSDHKGIN
RRGGGLTAPALTEKDKADIKLAAEMEVDYLAVSFPRDAADMNYARQLRDEAGGTAWLVAKIERAEAVADDETLDGLIKAS
DAVMVARGDLGVEIGDAELVGIQKKIILHARRHNKAVIVATQMMESMIQNPMPTRAEVSDVANAVLDYTDAVMLSAESAA
GAYPLEAVQAMARICVGAEKHPTSKTSSHRIGKEFERCDESIALATMYTANHFPGVKAIIALTESGYTPLIMSRIRSSVP
IYCFTPHREAQARAAMFRGVYTVPFDPASLAPNEVSQKAIDELVKRGVVEKGDWVILTKGDSYHTTGGTNGMKILHVGDP
QV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=500, Percent_Identity=36.2, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI33286420, Length=500, Percent_Identity=36.2, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI33286418, Length=499, Percent_Identity=36.4729458917836, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=34.4758064516129, Blast_Score=239, Evalue=5e-63,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=34.4758064516129, Blast_Score=239, Evalue=5e-63,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=36.3013698630137, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=36.3013698630137, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=34.8739495798319, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=34.8739495798319, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=33.6448598130841, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=56.9327731092437, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1787965, Length=462, Percent_Identity=37.2294372294372, Blast_Score=282, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=498, Percent_Identity=36.144578313253, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=35.9342915811088, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=35.9342915811088, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=35.9342915811088, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=35.6415478615071, Blast_Score=241, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6319279, Length=490, Percent_Identity=34.2857142857143, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=31.9672131147541, Blast_Score=223, Evalue=5e-59,
Organism=Drosophila melanogaster, GI28571814, Length=495, Percent_Identity=35.7575757575758, Blast_Score=265, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=36.1788617886179, Blast_Score=264, Evalue=8e-71,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=37.8048780487805, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24581235, Length=490, Percent_Identity=29.1836734693878, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24646914, Length=340, Percent_Identity=34.1176470588235, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52113; Mature: 51982

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRRTKIVATLGPASNSPEVLEQLILAGLDVARLNFSHGTPDEHKARAKLVRDLAAKHG
CCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCC
RFVALLGDLQGPKIRIAKFANKRIELKIGDQFTFSTSHPLTEGNQQVVGIDYPDLVKDCG
CEEEEEECCCCCEEEEEEECCCEEEEEECCEEEECCCCCCCCCCCEEEECCCHHHHHHCC
VGDELLLDDGRVVMRVETATATELNCVVTIGGPLSDHKGINRRGGGLTAPALTEKDKADI
CCCEEEECCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
KLAAEMEVDYLAVSFPRDAADMNYARQLRDEAGGTAWLVAKIERAEAVADDETLDGLIKA
EEEEECCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHHHCC
SDAVMVARGDLGVEIGDAELVGIQKKIILHARRHNKAVIVATQMMESMIQNPMPTRAEVS
CCEEEEEECCCCEEECCCEEEEHHHEEHEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDYTDAVMLSAESAAGAYPLEAVQAMARICVGAEKHPTSKTSSHRIGKEFERCD
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
ESIALATMYTANHFPGVKAIIALTESGYTPLIMSRIRSSVPIYCFTPHREAQARAAMFRG
CCEEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHCC
VYTVPFDPASLAPNEVSQKAIDELVKRGVVEKGDWVILTKGDSYHTTGGTNGMKILHVGD
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCCCEEEEECCC
PQV
CCC
>Mature Secondary Structure 
SVRRTKIVATLGPASNSPEVLEQLILAGLDVARLNFSHGTPDEHKARAKLVRDLAAKHG
CCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCC
RFVALLGDLQGPKIRIAKFANKRIELKIGDQFTFSTSHPLTEGNQQVVGIDYPDLVKDCG
CEEEEEECCCCCEEEEEEECCCEEEEEECCEEEECCCCCCCCCCCEEEECCCHHHHHHCC
VGDELLLDDGRVVMRVETATATELNCVVTIGGPLSDHKGINRRGGGLTAPALTEKDKADI
CCCEEEECCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
KLAAEMEVDYLAVSFPRDAADMNYARQLRDEAGGTAWLVAKIERAEAVADDETLDGLIKA
EEEEECCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHHHCC
SDAVMVARGDLGVEIGDAELVGIQKKIILHARRHNKAVIVATQMMESMIQNPMPTRAEVS
CCEEEEEECCCCEEECCCEEEEHHHEEHEEECCCCCEEEEHHHHHHHHHCCCCCCHHHHH
DVANAVLDYTDAVMLSAESAAGAYPLEAVQAMARICVGAEKHPTSKTSSHRIGKEFERCD
HHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
ESIALATMYTANHFPGVKAIIALTESGYTPLIMSRIRSSVPIYCFTPHREAQARAAMFRG
CCEEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHCC
VYTVPFDPASLAPNEVSQKAIDELVKRGVVEKGDWVILTKGDSYHTTGGTNGMKILHVGD
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCCCEEEEECCC
PQV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]