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The map label for this gene is cspA [H]

Identifier: 77460636

GI number: 77460636

Start: 4973624

End: 4974226

Strand: Direct

Name: cspA [H]

Synonym: Pfl01_4415

Alternate gene names: 77460636

Gene position: 4973624-4974226 (Clockwise)

Preceding gene: 77460617

Following gene: 77460640

Centisome position: 77.25

GC content: 59.7

Gene sequence:

>603_bases
ATGTTGAAAATCGTCCACCTGCTAATGGGCGCGGCAGCCTTGCTGCTGTCCTTCATACCCAGCCTGAAGTCCGAAGCGGT
ACCTTACCTGCAACAACCCGATGCACTTTACCTGGCCTTTTTCGGCCTGCTGAACCTGATCCTTGCGCCAGTGATCCCCT
ACTGGAACAAAGGTCCGCGCCAGCATCTGCAAAACCTGGTCAGCGCCTTGCTGGTCCTGACCGTTGTCCTGCAAACCCTG
ACCCTGATCGCGCCGATGCCTGTCATCGCCGGCCAGCCAGCCGTGCTGTTCAGCCTGGTGATCGCACTGGTCGCCGTGGT
TTTGCACCTGGCGGTCAGCTTCTATAAATCTTCACCGGCCGCCGCTACGCAAAGCTACGACATGAGCAACCGCGATACCG
GCACCGTCAAGTGGTTCAACACCTCCAAGGGCTTCGGTTTTATCTCCCGGGATTCCGGCGATGATATTTTCGTGCACTTT
CGCGCCATCCGTGGCGAAGGCCACCGCGTTCTGGTCGAAGGCCAGCGTGTGGAGTTCTCGGTGATGAACCGTGACAAGGG
CCTGCAGGCCGAAGACGTGATCGCCGCCCTGCCGCGCCGCTGA

Upstream 100 bases:

>100_bases
GGCTGATGCCGTGCACGTGTCCAACGCCTCTTATTGTTCCGAAGCGAACCGTGCGCCTTCAGCTCTTCCTTGTGAGCCGT
CATAACGTGAGTCAATAAAA

Downstream 100 bases:

>100_bases
TCCACGTCCCGCAATAAAAAAACCGCGAACAGCCTGGCTGTTCGCGGTTTTTTTAATGAATGCGTTTTTAACGGGAGCAT
GCCCGCGCACTTCTCAATAG

Product: cold-shock DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MLKIVHLLMGAAALLLSFIPSLKSEAVPYLQQPDALYLAFFGLLNLILAPVIPYWNKGPRQHLQNLVSALLVLTVVLQTL
TLIAPMPVIAGQPAVLFSLVIALVAVVLHLAVSFYKSSPAAATQSYDMSNRDTGTVKWFNTSKGFGFISRDSGDDIFVHF
RAIRGEGHRVLVEGQRVEFSVMNRDKGLQAEDVIAALPRR

Sequences:

>Translated_200_residues
MLKIVHLLMGAAALLLSFIPSLKSEAVPYLQQPDALYLAFFGLLNLILAPVIPYWNKGPRQHLQNLVSALLVLTVVLQTL
TLIAPMPVIAGQPAVLFSLVIALVAVVLHLAVSFYKSSPAAATQSYDMSNRDTGTVKWFNTSKGFGFISRDSGDDIFVHF
RAIRGEGHRVLVEGQRVEFSVMNRDKGLQAEDVIAALPRR
>Mature_200_residues
MLKIVHLLMGAAALLLSFIPSLKSEAVPYLQQPDALYLAFFGLLNLILAPVIPYWNKGPRQHLQNLVSALLVLTVVLQTL
TLIAPMPVIAGQPAVLFSLVIALVAVVLHLAVSFYKSSPAAATQSYDMSNRDTGTVKWFNTSKGFGFISRDSGDDIFVHF
RAIRGEGHRVLVEGQRVEFSVMNRDKGLQAEDVIAALPRR

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=63, Percent_Identity=58.7301587301587, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=55.8823529411765, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787834, Length=69, Percent_Identity=52.1739130434783, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788126, Length=63, Percent_Identity=55.5555555555556, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789979, Length=69, Percent_Identity=50.7246376811594, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI2367114, Length=69, Percent_Identity=52.1739130434783, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1787107, Length=60, Percent_Identity=50, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 21924; Mature: 21924

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIVHLLMGAAALLLSFIPSLKSEAVPYLQQPDALYLAFFGLLNLILAPVIPYWNKGPR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
QHLQNLVSALLVLTVVLQTLTLIAPMPVIAGQPAVLFSLVIALVAVVLHLAVSFYKSSPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AATQSYDMSNRDTGTVKWFNTSKGFGFISRDSGDDIFVHFRAIRGEGHRVLVEGQRVEFS
HHHCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEEEEEEEECCCCCEEEEECCEEEEE
VMNRDKGLQAEDVIAALPRR
EECCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MLKIVHLLMGAAALLLSFIPSLKSEAVPYLQQPDALYLAFFGLLNLILAPVIPYWNKGPR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
QHLQNLVSALLVLTVVLQTLTLIAPMPVIAGQPAVLFSLVIALVAVVLHLAVSFYKSSPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AATQSYDMSNRDTGTVKWFNTSKGFGFISRDSGDDIFVHFRAIRGEGHRVLVEGQRVEFS
HHHCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEEEEEEEECCCCCEEEEECCEEEEE
VMNRDKGLQAEDVIAALPRR
EECCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]