Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 77460628

GI number: 77460628

Start: 4966713

End: 4967774

Strand: Reverse

Name: ruvB

Synonym: Pfl01_4407

Alternate gene names: 77460628

Gene position: 4967774-4966713 (Counterclockwise)

Preceding gene: 77460629

Following gene: 77460627

Centisome position: 77.16

GC content: 60.45

Gene sequence:

>1062_bases
GTGATTGAAGCTGATCGTCTGATCGCCGCCACGCACAGTCCGCGTGAGCGCGAAGAAGTCCAGGATCGGGCGATACGTCC
CGTCAGCCTGGCCGAGTACATCGGCCAGCCGACCGTTCGCGAGCAGATGGAACTGTTTATCCAGGCCGCCCGTGGCCGTA
GCGAATCCCTCGACCACACCCTGATCTTCGGTCCGCCGGGGCTGGGTAAAACTACCTTGGCGAACATCATTGCTCAGGAA
ATGGGTGTGTCGATCAAGAGCACTTCCGGCCCGGTGCTGGAACGTCCGGGTGATCTGGCGGCGCTGTTGACCAATCTTGA
GCCGCACGACGTGCTGTTCATCGATGAAATCCATCGTCTGTCGCCGATCGTCGAAGAAGTGCTGTACCCGGCCATGGAAG
ATTTCCAGCTCGACATCATGATCGGTGAAGGTCCGGCGGCGCGTTCGATCAAACTTGATCTGCCACCGTTCACCCTGGTT
GGCGCGACGACGCGGGCGGGCATGCTGACCAACCCGTTGCGCGACCGTTTCGGCATCGTCCAGCGTCTCGAGTTCTACAG
CACCGCTGACCTGGCGACGATTGTCAGCCGTTCGGCGAATATCCTCGGCTTGCCGCTGGATCCGGAAGGGTCTTTCGAGA
TTGCCCGTCGTGCACGTGGCACGCCGCGGATCGCCAACCGACTGCTGCGTCGGGTGCGGGATTTCGCCGAAGTCCGGGCC
AAGGGCCACATCACCAAATCCGTCGCAGATCTGGCGTTGAACCTGCTGGATGTCGACGAGCATGGTTTCGATCATCAGGA
CCGGCGTCTGCTGTTGACCATGATCGAGAAGTTTGACGGCGGCCCGGTAGGCATTGACAGTCTGGCGGCGGCGATCAGCG
AAGAGCGCCACACCATTGAAGACGTGCTCGAGCCGTACCTGATTCAGCAGGGCTACATCATGCGTACGCCGCGGGGCAGG
GTGGTTACCCGTCACGCGTACCTGCATTTTGGTTTAAACATTCCGACACGAATGGGCGAGATGCCCGTGGTAGACGAGTT
TCTCGATGCCGTGGACGATTGA

Upstream 100 bases:

>100_bases
CTACAAGCCACAGGAAGCGAGCAAGGCGATTTCCGCGATCAAAGAGAAAGGCCTGAGCAGCGAAGACATGATTCGACGCG
CCCTGAAGGGAATGATTTAA

Downstream 100 bases:

>100_bases
TACACATTTATTGTCGATTTATTCAGCGTTTGTACTGTCACAGGACGGTACTTTTGCGGTAAAGCCTTCCAACAATGAAA
AACAGTTGCCTGGCCGGATT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MIEADRLIAATHSPREREEVQDRAIRPVSLAEYIGQPTVREQMELFIQAARGRSESLDHTLIFGPPGLGKTTLANIIAQE
MGVSIKSTSGPVLERPGDLAALLTNLEPHDVLFIDEIHRLSPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLV
GATTRAGMLTNPLRDRFGIVQRLEFYSTADLATIVSRSANILGLPLDPEGSFEIARRARGTPRIANRLLRRVRDFAEVRA
KGHITKSVADLALNLLDVDEHGFDHQDRRLLLTMIEKFDGGPVGIDSLAAAISEERHTIEDVLEPYLIQQGYIMRTPRGR
VVTRHAYLHFGLNIPTRMGEMPVVDEFLDAVDD

Sequences:

>Translated_353_residues
MIEADRLIAATHSPREREEVQDRAIRPVSLAEYIGQPTVREQMELFIQAARGRSESLDHTLIFGPPGLGKTTLANIIAQE
MGVSIKSTSGPVLERPGDLAALLTNLEPHDVLFIDEIHRLSPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLV
GATTRAGMLTNPLRDRFGIVQRLEFYSTADLATIVSRSANILGLPLDPEGSFEIARRARGTPRIANRLLRRVRDFAEVRA
KGHITKSVADLALNLLDVDEHGFDHQDRRLLLTMIEKFDGGPVGIDSLAAAISEERHTIEDVLEPYLIQQGYIMRTPRGR
VVTRHAYLHFGLNIPTRMGEMPVVDEFLDAVDD
>Mature_353_residues
MIEADRLIAATHSPREREEVQDRAIRPVSLAEYIGQPTVREQMELFIQAARGRSESLDHTLIFGPPGLGKTTLANIIAQE
MGVSIKSTSGPVLERPGDLAALLTNLEPHDVLFIDEIHRLSPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLV
GATTRAGMLTNPLRDRFGIVQRLEFYSTADLATIVSRSANILGLPLDPEGSFEIARRARGTPRIANRLLRRVRDFAEVRA
KGHITKSVADLALNLLDVDEHGFDHQDRRLLLTMIEKFDGGPVGIDSLAAAISEERHTIEDVLEPYLIQQGYIMRTPRGR
VVTRHAYLHFGLNIPTRMGEMPVVDEFLDAVDD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=343, Percent_Identity=72.0116618075802, Blast_Score=493, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_PSEPF (Q3K7W0)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350135.1
- ProteinModelPortal:   Q3K7W0
- SMR:   Q3K7W0
- STRING:   Q3K7W0
- GeneID:   3713310
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4407
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   PFLU205922:PFL_4407-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 39261; Mature: 39261

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEADRLIAATHSPREREEVQDRAIRPVSLAEYIGQPTVREQMELFIQAARGRSESLDHT
CCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCE
LIFGPPGLGKTTLANIIAQEMGVSIKSTSGPVLERPGDLAALLTNLEPHDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHH
SPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGMLTNPLRDRFGIV
HHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEECCCHHCCHHHHHHHHHHHHH
QRLEFYSTADLATIVSRSANILGLPLDPEGSFEIARRARGTPRIANRLLRRVRDFAEVRA
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KGHITKSVADLALNLLDVDEHGFDHQDRRLLLTMIEKFDGGPVGIDSLAAAISEERHTIE
HCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DVLEPYLIQQGYIMRTPRGRVVTRHAYLHFGLNIPTRMGEMPVVDEFLDAVDD
HHHHHHHHCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MIEADRLIAATHSPREREEVQDRAIRPVSLAEYIGQPTVREQMELFIQAARGRSESLDHT
CCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCE
LIFGPPGLGKTTLANIIAQEMGVSIKSTSGPVLERPGDLAALLTNLEPHDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHH
SPIVEEVLYPAMEDFQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGMLTNPLRDRFGIV
HHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEECCCHHCCHHHHHHHHHHHHH
QRLEFYSTADLATIVSRSANILGLPLDPEGSFEIARRARGTPRIANRLLRRVRDFAEVRA
HHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KGHITKSVADLALNLLDVDEHGFDHQDRRLLLTMIEKFDGGPVGIDSLAAAISEERHTIE
HCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DVLEPYLIQQGYIMRTPRGRVVTRHAYLHFGLNIPTRMGEMPVVDEFLDAVDD
HHHHHHHHCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA