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The map label for this gene is pitA [H]

Identifier: 77460540

GI number: 77460540

Start: 4875225

End: 4876838

Strand: Direct

Name: pitA [H]

Synonym: Pfl01_4319

Alternate gene names: 77460540

Gene position: 4875225-4876838 (Clockwise)

Preceding gene: 77460539

Following gene: 77460542

Centisome position: 75.72

GC content: 62.76

Gene sequence:

>1614_bases
ATGGCTACTCCCTCCCTGACCGCCAGCCTGGCCCCCACTGCCAGTGGCAGGCCGGCGCTCGACAAAAAAACCGGCCCCTT
TACTTACGTGGTGTTCTTCGCCGTGCTGGCCATGGGGATGCTGTTCACCGCCTACAGTCTGATGCATGACATGCACGAAC
TCGGCACGGTGGTCACCACCTGGACGCCGTTCCTGCTGCTCGGCGTGGCGCTGTTGATTGCACTGGGTTTTGAATTCGTC
AACGGTTTCCATGACACCGCCAACGCCGTGGCCACGGTGATCTACACCCACTCGCTGCCGCCGAATGTGGCCGTGGTGTG
GTCGGGTTTCTTCAACTTCCTCGGGGTACTGCTTTCGAGCGGCGCGGTGGCGTTCGGCATCATTGCGCTGTTGCCGGTGG
AGCTGATTCTGCAAGTGGGTTCGTCCGCCGGTTTCGCGATGATCTTCGCCCTGCTGATCGCGGCGATCCTGTGGAACCTC
GGCACCTGGTGGCTGGGCCTGCCGGCCTCGTCGTCGCACACGCTGATCGGTTCGATCATCGGCGTCGGCGTGGCCAACGC
GTTGATGCATGGTCGCGACGGCACCAGCGGTGTGGACTGGGCCCAGGCGACCAAGATCGGTTACGCACTGCTGCTGTCGC
CGCTGGTGGGTTTCGGTTGTGCGGCGCTGTTGCTGCTGGCGTTGCGCGCGTTCGTAAAGAACCGCTCGCTGTACAAGGCA
CCGGAAGGCAATACCCCGCCGCCGTGGTGGATTCGTGGTTTGCTGATCCTGACCTGCACCGGCGTGTCGTTCGCACACGG
TTCCAACGACGGCCAGAAAGGCATGGGCCTGATCATGCTGATTCTGGTCGGCACTCTGCCGATGGCTTATGCACTGAACC
GCACCATGCCGGAAGAACAGTCGCTGCAATTTGCCGCCGTCGCCCAAGTGACCCAGCAAGCGCTGGTAAAAAGTGCGCCA
CTGCCGGCACCGGCCGATCCACGCGCCGTGCTCTCGGATTACATGCGCAGCAAGCAAGCCACGCCGCAACTGATCCCCGC
CCTCGCCTCGCTGACCGGACACATCGGTGAAGAAGTCAAAGGCTACGGCTCGCTGGCGAAAGTCCCGGCCGAGGCCATGG
GTAACGTGCGTAACGACATGTACCTGGCCAGCGAAACCATTCGCCTGATGGACAAGAACAAGGTCGGCAGCTTCGACGCC
GACACCAGCGGCAAGCTGCAACTGTTCAAACAGCAGATCGACAACGCCACGCGGTTCATTCCGCTGTGGGTGAAGATCGC
CGTGGCGATTGCCTTGGGTCTGGGCACCATGGTCGGCTGGAAGCGGATCGTGGTCACGGTCGGCGAGAAGATCGGCAAGA
CCCACCTGACCTACGCCCAGGGCGCCTCGGCGGAAACCGTGGCGATGCTGACCATCGGCGCCGCCGACATGTTCGGCTTG
CCGGTCTCGACCACCCATGTGTTGTCCTCGGGCGTGGCCGGGACCATGGTCGCCAACGGTGGCGGCTTGCAGATGAAGAC
CATCCGCAATCTGCTGATGGCGTGGGTGCTGACCTTGCCGGCGGCGATTGTGCTGTCGGGCAGCCTGTATTGGCTGTTTA
CCCAGATCTTCTGA

Upstream 100 bases:

>100_bases
GCGCGACTAATCCGCCAAACACTGATCTGTCGCAGCCTCTCTGTAATATTTAGCAACGATCATGGGCAACACTCCTTCCC
CCGTTCAGGATTGTCCGCCC

Downstream 100 bases:

>100_bases
TCCATCCCAATCCCTGTGAGAGCCGTTGCTCCCACAGGGATTTGCGGCAAGAATCAGAGCGTCGAACGAATCAACTCCCC
CAGCCAGTCCATGAACACCC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MATPSLTASLAPTASGRPALDKKTGPFTYVVFFAVLAMGMLFTAYSLMHDMHELGTVVTTWTPFLLLGVALLIALGFEFV
NGFHDTANAVATVIYTHSLPPNVAVVWSGFFNFLGVLLSSGAVAFGIIALLPVELILQVGSSAGFAMIFALLIAAILWNL
GTWWLGLPASSSHTLIGSIIGVGVANALMHGRDGTSGVDWAQATKIGYALLLSPLVGFGCAALLLLALRAFVKNRSLYKA
PEGNTPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPEEQSLQFAAVAQVTQQALVKSAP
LPAPADPRAVLSDYMRSKQATPQLIPALASLTGHIGEEVKGYGSLAKVPAEAMGNVRNDMYLASETIRLMDKNKVGSFDA
DTSGKLQLFKQQIDNATRFIPLWVKIAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGASAETVAMLTIGAADMFGL
PVSTTHVLSSGVAGTMVANGGGLQMKTIRNLLMAWVLTLPAAIVLSGSLYWLFTQIF

Sequences:

>Translated_537_residues
MATPSLTASLAPTASGRPALDKKTGPFTYVVFFAVLAMGMLFTAYSLMHDMHELGTVVTTWTPFLLLGVALLIALGFEFV
NGFHDTANAVATVIYTHSLPPNVAVVWSGFFNFLGVLLSSGAVAFGIIALLPVELILQVGSSAGFAMIFALLIAAILWNL
GTWWLGLPASSSHTLIGSIIGVGVANALMHGRDGTSGVDWAQATKIGYALLLSPLVGFGCAALLLLALRAFVKNRSLYKA
PEGNTPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPEEQSLQFAAVAQVTQQALVKSAP
LPAPADPRAVLSDYMRSKQATPQLIPALASLTGHIGEEVKGYGSLAKVPAEAMGNVRNDMYLASETIRLMDKNKVGSFDA
DTSGKLQLFKQQIDNATRFIPLWVKIAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGASAETVAMLTIGAADMFGL
PVSTTHVLSSGVAGTMVANGGGLQMKTIRNLLMAWVLTLPAAIVLSGSLYWLFTQIF
>Mature_536_residues
ATPSLTASLAPTASGRPALDKKTGPFTYVVFFAVLAMGMLFTAYSLMHDMHELGTVVTTWTPFLLLGVALLIALGFEFVN
GFHDTANAVATVIYTHSLPPNVAVVWSGFFNFLGVLLSSGAVAFGIIALLPVELILQVGSSAGFAMIFALLIAAILWNLG
TWWLGLPASSSHTLIGSIIGVGVANALMHGRDGTSGVDWAQATKIGYALLLSPLVGFGCAALLLLALRAFVKNRSLYKAP
EGNTPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPEEQSLQFAAVAQVTQQALVKSAPL
PAPADPRAVLSDYMRSKQATPQLIPALASLTGHIGEEVKGYGSLAKVPAEAMGNVRNDMYLASETIRLMDKNKVGSFDAD
TSGKLQLFKQQIDNATRFIPLWVKIAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGASAETVAMLTIGAADMFGLP
VSTTHVLSSGVAGTMVANGGGLQMKTIRNLLMAWVLTLPAAIVLSGSLYWLFTQIF

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=483, Percent_Identity=44.5134575569358, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1789360, Length=481, Percent_Identity=42.4116424116424, Blast_Score=371, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 56749; Mature: 56617

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPSLTASLAPTASGRPALDKKTGPFTYVVFFAVLAMGMLFTAYSLMHDMHELGTVVTT
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTPFLLLGVALLIALGFEFVNGFHDTANAVATVIYTHSLPPNVAVVWSGFFNFLGVLLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHC
GAVAFGIIALLPVELILQVGSSAGFAMIFALLIAAILWNLGTWWLGLPASSSHTLIGSII
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHHH
GVGVANALMHGRDGTSGVDWAQATKIGYALLLSPLVGFGCAALLLLALRAFVKNRSLYKA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PEGNTPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPEEQ
CCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
SLQFAAVAQVTQQALVKSAPLPAPADPRAVLSDYMRSKQATPQLIPALASLTGHIGEEVK
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GYGSLAKVPAEAMGNVRNDMYLASETIRLMDKNKVGSFDADTSGKLQLFKQQIDNATRFI
CCCHHHHCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHH
PLWVKIAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGASAETVAMLTIGAADMFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCHHHHCC
PVSTTHVLSSGVAGTMVANGGGLQMKTIRNLLMAWVLTLPAAIVLSGSLYWLFTQIF
CCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
ATPSLTASLAPTASGRPALDKKTGPFTYVVFFAVLAMGMLFTAYSLMHDMHELGTVVTT
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTPFLLLGVALLIALGFEFVNGFHDTANAVATVIYTHSLPPNVAVVWSGFFNFLGVLLSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHC
GAVAFGIIALLPVELILQVGSSAGFAMIFALLIAAILWNLGTWWLGLPASSSHTLIGSII
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHHH
GVGVANALMHGRDGTSGVDWAQATKIGYALLLSPLVGFGCAALLLLALRAFVKNRSLYKA
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PEGNTPPPWWIRGLLILTCTGVSFAHGSNDGQKGMGLIMLILVGTLPMAYALNRTMPEEQ
CCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
SLQFAAVAQVTQQALVKSAPLPAPADPRAVLSDYMRSKQATPQLIPALASLTGHIGEEVK
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GYGSLAKVPAEAMGNVRNDMYLASETIRLMDKNKVGSFDADTSGKLQLFKQQIDNATRFI
CCCHHHHCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHH
PLWVKIAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGASAETVAMLTIGAADMFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCHHHHCC
PVSTTHVLSSGVAGTMVANGGGLQMKTIRNLLMAWVLTLPAAIVLSGSLYWLFTQIF
CCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]