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The map label for this gene is gatA [H]

Identifier: 77460525

GI number: 77460525

Start: 4860451

End: 4861968

Strand: Reverse

Name: gatA [H]

Synonym: Pfl01_4304

Alternate gene names: 77460525

Gene position: 4861968-4860451 (Counterclockwise)

Preceding gene: 77460526

Following gene: 77460521

Centisome position: 75.52

GC content: 60.54

Gene sequence:

>1518_bases
ATGAAATTCCGCTTTAAACGCAGCATGAATGCGCTGGCGCCGGCCATCCTGCTTTCGCTGAACAGTAGCGTCGCCCTCGC
AGCGTCCTCCAGTGCGCCACTTGAATACGCAAGCGCCAGCGAGCTGAGTGCAATGATGGCCAGCAACGAACTGACTTCGG
TCGCGCTGGTTCAACACCTCATCACACGCATTGCGGATCTGGACAAGCAAGGCCCGACCCTCAATGCCATCATCGAAATA
AACCCCCAGGCTCTCGACATCGCGACCGCGCTGGATGAAGAGCGTGCGCAAGGCAACCTCCGTGGCCCGCTGCACGGAAT
CCCGGTCCTGTTGAAGGACAACTTCGATACTGCCGACAGCATGCAAACCGGTGCCGGTTCGTTGGCCATGATCGGGCAGC
CGGCGGCTCAGGATGCGTTCACGGTCAAACAATTGCGCGATGCCGGCGCCATCCTGCTGGGCAAGGCCAACATGAGCGAG
TGGTCGGGAATGCGGGACTTGTCACTGCCCTTGGGGTGGAGCGGGCGCGGTGGTCAGGGGAAAAATCCTCATGTCCTCAG
CGAAAGTACTTGTGGTTCCAGTTCAGGTTCGGCGGCAGCGGTTGCCGCAGGGCTCTCTCCACTGGCCATGGGAACTGAAA
CCAACGGCTCGATCAGTTGCCCGGCGTCGGCCAATGGTGTGGTTGGCGTCAAACCGTCGCTCGGATTGTTCAGCCGCGCC
GGCATCGTGCCAATCACCCGCTTGCAAGACACGCCGGGCACAATGACACGTACCGTGCGGGATGCCGCGCTGTTGTTCAA
CGTCATACAAGGCGTTGACGCCTCGGATAGCGCCACTGCTGACGCGCCCACTGGCGTCGACTACACAGCATTGCTTTCCA
GTGAAGCCCTGCAAGGCAAACGGATCGGTTATCCGATTGCATACACTGGATCCCACGCTACGGTACTGAACCCGAGTCTG
GAGATGCTTGCCGCGATGGCCACCCTGGAAGAGCAGGGCGCTACGCTGGTCCCGCTGACTGTGCGTCTGCCGGACATCGA
TGACTACGTTAACGGCCTGATGGGCGCGATGAAGCATGAGCTGCCCGAATATCTCGCCAGCCGCCAGGGCTTGCCGGTCG
ACTCCTTGCAGGCTCTGATCGACTTCAACCTGGCCAATCCCGGTGAGGAGCAACATGGCCAGCAGACGCTCGAGACCATC
AACGCGTCGGCGCTTTCCCACCAGGAGGCGAGCGCACTGATTACCACCATCAGCCAGAACTTCAAGGACGCTATCGATGA
GCAGGTGCGTGAGCACAATCTGGATGCCCTGTTCGCCGAAGCGGACGGCTTTTCCCAGTTCTCCGCCGCCACCGCAGGCT
ACCCGGCAATCACCTTGCCCTCGGGCATGAACGATGACGCCACGCCGACCTCCGTGACCTTCTACGGCCAGCAGTGGAGC
GAGCCACAGTTGTTTGCCATGGCGTACAGCTACGAACAGGCTTCGATGGAATTGCGTCATCCGGGGTTCAAGGAGTAA

Upstream 100 bases:

>100_bases
GCACTGGTTCTTGTTGCTGACAGCCGTTTCCTGCGGCTTCGGTAGGCTTGGTCGCGAGTGAAATGACTCACTCGTGATTG
GCTTAAACAAGGATTAGAAA

Downstream 100 bases:

>100_bases
ACACCTTGTTGGCAGTGCGTTGTCTTCGACTATTTCAAGCACTGCCGTATCAAGCGAACACCACCTCAATTCGGCCCTCT
TTGCGAGAGCGGGGGGCCGG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MKFRFKRSMNALAPAILLSLNSSVALAASSSAPLEYASASELSAMMASNELTSVALVQHLITRIADLDKQGPTLNAIIEI
NPQALDIATALDEERAQGNLRGPLHGIPVLLKDNFDTADSMQTGAGSLAMIGQPAAQDAFTVKQLRDAGAILLGKANMSE
WSGMRDLSLPLGWSGRGGQGKNPHVLSESTCGSSSGSAAAVAAGLSPLAMGTETNGSISCPASANGVVGVKPSLGLFSRA
GIVPITRLQDTPGTMTRTVRDAALLFNVIQGVDASDSATADAPTGVDYTALLSSEALQGKRIGYPIAYTGSHATVLNPSL
EMLAAMATLEEQGATLVPLTVRLPDIDDYVNGLMGAMKHELPEYLASRQGLPVDSLQALIDFNLANPGEEQHGQQTLETI
NASALSHQEASALITTISQNFKDAIDEQVREHNLDALFAEADGFSQFSAATAGYPAITLPSGMNDDATPTSVTFYGQQWS
EPQLFAMAYSYEQASMELRHPGFKE

Sequences:

>Translated_505_residues
MKFRFKRSMNALAPAILLSLNSSVALAASSSAPLEYASASELSAMMASNELTSVALVQHLITRIADLDKQGPTLNAIIEI
NPQALDIATALDEERAQGNLRGPLHGIPVLLKDNFDTADSMQTGAGSLAMIGQPAAQDAFTVKQLRDAGAILLGKANMSE
WSGMRDLSLPLGWSGRGGQGKNPHVLSESTCGSSSGSAAAVAAGLSPLAMGTETNGSISCPASANGVVGVKPSLGLFSRA
GIVPITRLQDTPGTMTRTVRDAALLFNVIQGVDASDSATADAPTGVDYTALLSSEALQGKRIGYPIAYTGSHATVLNPSL
EMLAAMATLEEQGATLVPLTVRLPDIDDYVNGLMGAMKHELPEYLASRQGLPVDSLQALIDFNLANPGEEQHGQQTLETI
NASALSHQEASALITTISQNFKDAIDEQVREHNLDALFAEADGFSQFSAATAGYPAITLPSGMNDDATPTSVTFYGQQWS
EPQLFAMAYSYEQASMELRHPGFKE
>Mature_505_residues
MKFRFKRSMNALAPAILLSLNSSVALAASSSAPLEYASASELSAMMASNELTSVALVQHLITRIADLDKQGPTLNAIIEI
NPQALDIATALDEERAQGNLRGPLHGIPVLLKDNFDTADSMQTGAGSLAMIGQPAAQDAFTVKQLRDAGAILLGKANMSE
WSGMRDLSLPLGWSGRGGQGKNPHVLSESTCGSSSGSAAAVAAGLSPLAMGTETNGSISCPASANGVVGVKPSLGLFSRA
GIVPITRLQDTPGTMTRTVRDAALLFNVIQGVDASDSATADAPTGVDYTALLSSEALQGKRIGYPIAYTGSHATVLNPSL
EMLAAMATLEEQGATLVPLTVRLPDIDDYVNGLMGAMKHELPEYLASRQGLPVDSLQALIDFNLANPGEEQHGQQTLETI
NASALSHQEASALITTISQNFKDAIDEQVREHNLDALFAEADGFSQFSAATAGYPAITLPSGMNDDATPTSVTFYGQQWS
EPQLFAMAYSYEQASMELRHPGFKE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=30.1587301587302, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI195972892, Length=253, Percent_Identity=29.2490118577075, Blast_Score=91, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=233, Percent_Identity=29.6137339055794, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17537465, Length=490, Percent_Identity=24.2857142857143, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=268, Percent_Identity=27.9850746268657, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=234, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=417, Percent_Identity=25.4196642685851, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=211, Percent_Identity=31.2796208530806, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648113, Length=250, Percent_Identity=36, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21356731, Length=268, Percent_Identity=30.5970149253731, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24644968, Length=325, Percent_Identity=29.5384615384615, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550774, Length=265, Percent_Identity=29.4339622641509, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648435, Length=265, Percent_Identity=29.4339622641509, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648437, Length=265, Percent_Identity=29.4339622641509, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648441, Length=194, Percent_Identity=30.9278350515464, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24648439, Length=194, Percent_Identity=30.9278350515464, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI161078093, Length=277, Percent_Identity=29.6028880866426, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24652985, Length=254, Percent_Identity=27.9527559055118, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922090, Length=254, Percent_Identity=27.9527559055118, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652981, Length=254, Percent_Identity=27.9527559055118, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652983, Length=254, Percent_Identity=27.9527559055118, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 52997; Mature: 52997

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFRFKRSMNALAPAILLSLNSSVALAASSSAPLEYASASELSAMMASNELTSVALVQHL
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
ITRIADLDKQGPTLNAIIEINPQALDIATALDEERAQGNLRGPLHGIPVLLKDNFDTADS
HHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHH
MQTGAGSLAMIGQPAAQDAFTVKQLRDAGAILLGKANMSEWSGMRDLSLPLGWSGRGGQG
HHCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
KNPHVLSESTCGSSSGSAAAVAAGLSPLAMGTETNGSISCPASANGVVGVKPSLGLFSRA
CCCCEECCCCCCCCCCCHHHHHHCCCHHEECCCCCCCEECCCCCCCEEECCCCCCHHHCC
GIVPITRLQDTPGTMTRTVRDAALLFNVIQGVDASDSATADAPTGVDYTALLSSEALQGK
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
RIGYPIAYTGSHATVLNPSLEMLAAMATLEEQGATLVPLTVRLPDIDDYVNGLMGAMKHE
CCCCCEEECCCCCEEECCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHH
LPEYLASRQGLPVDSLQALIDFNLANPGEEQHGQQTLETINASALSHQEASALITTISQN
HHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKDAIDEQVREHNLDALFAEADGFSQFSAATAGYPAITLPSGMNDDATPTSVTFYGQQWS
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCC
EPQLFAMAYSYEQASMELRHPGFKE
CCEEEEEEEHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFRFKRSMNALAPAILLSLNSSVALAASSSAPLEYASASELSAMMASNELTSVALVQHL
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
ITRIADLDKQGPTLNAIIEINPQALDIATALDEERAQGNLRGPLHGIPVLLKDNFDTADS
HHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHH
MQTGAGSLAMIGQPAAQDAFTVKQLRDAGAILLGKANMSEWSGMRDLSLPLGWSGRGGQG
HHCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCC
KNPHVLSESTCGSSSGSAAAVAAGLSPLAMGTETNGSISCPASANGVVGVKPSLGLFSRA
CCCCEECCCCCCCCCCCHHHHHHCCCHHEECCCCCCCEECCCCCCCEEECCCCCCHHHCC
GIVPITRLQDTPGTMTRTVRDAALLFNVIQGVDASDSATADAPTGVDYTALLSSEALQGK
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
RIGYPIAYTGSHATVLNPSLEMLAAMATLEEQGATLVPLTVRLPDIDDYVNGLMGAMKHE
CCCCCEEECCCCCEEECCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHH
LPEYLASRQGLPVDSLQALIDFNLANPGEEQHGQQTLETINASALSHQEASALITTISQN
HHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKDAIDEQVREHNLDALFAEADGFSQFSAATAGYPAITLPSGMNDDATPTSVTFYGQQWS
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCC
EPQLFAMAYSYEQASMELRHPGFKE
CCEEEEEEEHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA