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The map label for this gene is rstB [H]

Identifier: 77460465

GI number: 77460465

Start: 4785722

End: 4787332

Strand: Reverse

Name: rstB [H]

Synonym: Pfl01_4244

Alternate gene names: 77460465

Gene position: 4787332-4785722 (Counterclockwise)

Preceding gene: 77460466

Following gene: 77460462

Centisome position: 74.36

GC content: 65.43

Gene sequence:

>1611_bases
GTGAACTCGATCTTCCTGCGCATCTATGGCGGCATGTGCGCGGCGCTGATTCTGGTGGCGGTGCTCGGTGTGCTGGCGCT
GCACCTGCTCAACCAGGTGCGCAGCGAGCAATACCGTGAGCGTCTGGCCCACGGCACGTTTTCGCTGATGGCCGACAACC
TGCAACCGATGAACGAAACCGAGCGCCATCGCGCCTTGCTGGTGTGGGAGCGGCTGCTCGGGATTCCGCTGGCGCTGAAG
ACCTTCACCCAGACCGATCTCGACCTGACCCAGCGCACTCGCGTGCAGCGCGGCCAGGCCCTGGTGGAGCAGACCGGCCC
GCACGCGGCCAAGGTCTATCGGCTGGTCAGCGACAAGGATCAACTGGTGCTGGTGGGCGAGGTGCAGCAGATCAGCGAGC
AACTGGCCCGGGCGACCATCTACCTGTTGGCCGATGAGCTGGTGCGGGTGCCCGTGGGCGAACAGCCCAAACGACTGGCG
CAGTTGAAAGAAGACAAAGGCTTCGGTTTCGATTTGCGACTGGTTACGGTCAGCGATGCCGACATGGACGAAGACCAGAG
CCGTCGGGTGTCCGAGGGCGATACGGTGATGGCGCTGGGCAAGGGCGGCGATTCGATCCGGGTGTTCGCCGGCATGGTCG
GCACGCCGTGGGTGCTGGAAATCGGGCCGCTGTATCAGATGAACCCGTACCCGCCGGAATGGCTGGTGCTGATCGCAGCC
CTCGGCCTGACCCTGATCGGCCTGATTGTCTATCTATTGGTCCGCCAGCTCGAACGGCGTTTGCGTGGACTCGAAGCTGC
CGCCACGCGCATCGCCAAAGGCAGCCTGGAAACCCGGGTGCCGGCCCGTGGTGCCGACTCGGTGGGCAAGCTGGCAGCGG
CGTTCAACGGCATGGCCGAGCACTTGCAGCAGTTGCTCGCCATTCAGCGTGAACTGGTGCGCGCGGTGTCCCACGAACTG
CGCACGCCGGTGGCGCGTCTGCGTTTCGGGCTGGAGATGATCGGCTCGGCCACCACCCCGCAGGCGCTGGAAAAATACCG
CGAGGGCATGGACCACGACATCGAGGACCTCGACAAGCTGGTCGACGAAATGCTCACGTACGCCAGACTGGAACAGGGCT
CGCCGGCGCTGACCTTCCAGCGCATCGACCTGGATGCGCTGGTCAATCAGGTGATCGAAGAGCTGGCGCCTCTGCGCGCC
GAGGTCACGGTGCAGCGCGGTCTGTGCCTGTCGGCGGCGGATTGCGATGATGCCTGGGTCGAGGCCGAGCCGCGTTACCT
GCACCGCGCCTTGCAGAATCTGGTGAGCAACGCCATGCGTCATGCGCGTTCGAAGGTCACGGTCAGTTATCAGGTCGGGC
AGATGCGTTGCCGGGTGGATGTCGAGGACGACGGCCCCGGCGTGCCGGAAACCGCCTGGGAGAAAATCTTCACGCCGTTC
CTGCGTCTGGACGACAGCCGCACCCGGGCGTCCGGCGGACACGGGCTGGGCCTGTCGATCGTGCGGCGGATCATCCACTG
GCACGACGGCCGGGCGCTGATTGGCAAGAGCAAGAGCCTGGGCGGGGCGTGTTTCAGTCTGAGCTGGCCGAGGAATCAGG
AGCGGCGCTGA

Upstream 100 bases:

>100_bases
GCCCGAAAATCGGCGACGACCCCGATCACCCGCGCCTGATCAAGACCGTGCGCAGCAAAGGCTACCTGTTCGTGCCGGAA
GCCTGCGCAGAACTGCCGCT

Downstream 100 bases:

>100_bases
AACTGCTTACGCCTGGATGCTGACCAGACTGAGCAACTGCCCGTCCTGCACGCCGAATTGCGCATCGAGCTCCGTGCCGT
TGCGCCATTCGCTGGAGAGA

Product: periplasmic sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MNSIFLRIYGGMCAALILVAVLGVLALHLLNQVRSEQYRERLAHGTFSLMADNLQPMNETERHRALLVWERLLGIPLALK
TFTQTDLDLTQRTRVQRGQALVEQTGPHAAKVYRLVSDKDQLVLVGEVQQISEQLARATIYLLADELVRVPVGEQPKRLA
QLKEDKGFGFDLRLVTVSDADMDEDQSRRVSEGDTVMALGKGGDSIRVFAGMVGTPWVLEIGPLYQMNPYPPEWLVLIAA
LGLTLIGLIVYLLVRQLERRLRGLEAAATRIAKGSLETRVPARGADSVGKLAAAFNGMAEHLQQLLAIQRELVRAVSHEL
RTPVARLRFGLEMIGSATTPQALEKYREGMDHDIEDLDKLVDEMLTYARLEQGSPALTFQRIDLDALVNQVIEELAPLRA
EVTVQRGLCLSAADCDDAWVEAEPRYLHRALQNLVSNAMRHARSKVTVSYQVGQMRCRVDVEDDGPGVPETAWEKIFTPF
LRLDDSRTRASGGHGLGLSIVRRIIHWHDGRALIGKSKSLGGACFSLSWPRNQERR

Sequences:

>Translated_536_residues
MNSIFLRIYGGMCAALILVAVLGVLALHLLNQVRSEQYRERLAHGTFSLMADNLQPMNETERHRALLVWERLLGIPLALK
TFTQTDLDLTQRTRVQRGQALVEQTGPHAAKVYRLVSDKDQLVLVGEVQQISEQLARATIYLLADELVRVPVGEQPKRLA
QLKEDKGFGFDLRLVTVSDADMDEDQSRRVSEGDTVMALGKGGDSIRVFAGMVGTPWVLEIGPLYQMNPYPPEWLVLIAA
LGLTLIGLIVYLLVRQLERRLRGLEAAATRIAKGSLETRVPARGADSVGKLAAAFNGMAEHLQQLLAIQRELVRAVSHEL
RTPVARLRFGLEMIGSATTPQALEKYREGMDHDIEDLDKLVDEMLTYARLEQGSPALTFQRIDLDALVNQVIEELAPLRA
EVTVQRGLCLSAADCDDAWVEAEPRYLHRALQNLVSNAMRHARSKVTVSYQVGQMRCRVDVEDDGPGVPETAWEKIFTPF
LRLDDSRTRASGGHGLGLSIVRRIIHWHDGRALIGKSKSLGGACFSLSWPRNQERR
>Mature_536_residues
MNSIFLRIYGGMCAALILVAVLGVLALHLLNQVRSEQYRERLAHGTFSLMADNLQPMNETERHRALLVWERLLGIPLALK
TFTQTDLDLTQRTRVQRGQALVEQTGPHAAKVYRLVSDKDQLVLVGEVQQISEQLARATIYLLADELVRVPVGEQPKRLA
QLKEDKGFGFDLRLVTVSDADMDEDQSRRVSEGDTVMALGKGGDSIRVFAGMVGTPWVLEIGPLYQMNPYPPEWLVLIAA
LGLTLIGLIVYLLVRQLERRLRGLEAAATRIAKGSLETRVPARGADSVGKLAAAFNGMAEHLQQLLAIQRELVRAVSHEL
RTPVARLRFGLEMIGSATTPQALEKYREGMDHDIEDLDKLVDEMLTYARLEQGSPALTFQRIDLDALVNQVIEELAPLRA
EVTVQRGLCLSAADCDDAWVEAEPRYLHRALQNLVSNAMRHARSKVTVSYQVGQMRCRVDVEDDGPGVPETAWEKIFTPF
LRLDDSRTRASGGHGLGLSIVRRIIHWHDGRALIGKSKSLGGACFSLSWPRNQERR

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=405, Percent_Identity=31.8518518518519, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1790346, Length=263, Percent_Identity=33.0798479087452, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789808, Length=271, Percent_Identity=33.210332103321, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788393, Length=266, Percent_Identity=31.9548872180451, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI87082128, Length=306, Percent_Identity=28.1045751633987, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786600, Length=232, Percent_Identity=29.7413793103448, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786783, Length=300, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788549, Length=234, Percent_Identity=26.9230769230769, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=28.8793103448276, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790861, Length=250, Percent_Identity=28, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI145693157, Length=243, Percent_Identity=23.8683127572016, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790551, Length=310, Percent_Identity=26.4516129032258, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788279, Length=287, Percent_Identity=25.7839721254355, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790300, Length=244, Percent_Identity=25.8196721311475, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=26.7241379310345, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081816, Length=342, Percent_Identity=24.2690058479532, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1789403, Length=300, Percent_Identity=29, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789149, Length=325, Percent_Identity=22.1538461538462, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI48994928, Length=224, Percent_Identity=28.125, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59749; Mature: 59749

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIFLRIYGGMCAALILVAVLGVLALHLLNQVRSEQYRERLAHGTFSLMADNLQPMNET
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHH
ERHRALLVWERLLGIPLALKTFTQTDLDLTQRTRVQRGQALVEQTGPHAAKVYRLVSDKD
HHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
QLVLVGEVQQISEQLARATIYLLADELVRVPVGEQPKRLAQLKEDKGFGFDLRLVTVSDA
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEECCC
DMDEDQSRRVSEGDTVMALGKGGDSIRVFAGMVGTPWVLEIGPLYQMNPYPPEWLVLIAA
CCCCHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHH
LGLTLIGLIVYLLVRQLERRLRGLEAAATRIAKGSLETRVPARGADSVGKLAAAFNGMAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HLQQLLAIQRELVRAVSHELRTPVARLRFGLEMIGSATTPQALEKYREGMDHDIEDLDKL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHH
VDEMLTYARLEQGSPALTFQRIDLDALVNQVIEELAPLRAEVTVQRGLCLSAADCDDAWV
HHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCHHHHHHHHCCCEEECCCCCCCCC
EAEPRYLHRALQNLVSNAMRHARSKVTVSYQVGQMRCRVDVEDDGPGVPETAWEKIFTPF
CCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHH
LRLDDSRTRASGGHGLGLSIVRRIIHWHDGRALIGKSKSLGGACFSLSWPRNQERR
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MNSIFLRIYGGMCAALILVAVLGVLALHLLNQVRSEQYRERLAHGTFSLMADNLQPMNET
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHH
ERHRALLVWERLLGIPLALKTFTQTDLDLTQRTRVQRGQALVEQTGPHAAKVYRLVSDKD
HHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
QLVLVGEVQQISEQLARATIYLLADELVRVPVGEQPKRLAQLKEDKGFGFDLRLVTVSDA
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEEECCC
DMDEDQSRRVSEGDTVMALGKGGDSIRVFAGMVGTPWVLEIGPLYQMNPYPPEWLVLIAA
CCCCHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHH
LGLTLIGLIVYLLVRQLERRLRGLEAAATRIAKGSLETRVPARGADSVGKLAAAFNGMAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HLQQLLAIQRELVRAVSHELRTPVARLRFGLEMIGSATTPQALEKYREGMDHDIEDLDKL
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHH
VDEMLTYARLEQGSPALTFQRIDLDALVNQVIEELAPLRAEVTVQRGLCLSAADCDDAWV
HHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCHHHHHHHHCCCEEECCCCCCCCC
EAEPRYLHRALQNLVSNAMRHARSKVTVSYQVGQMRCRVDVEDDGPGVPETAWEKIFTPF
CCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHH
LRLDDSRTRASGGHGLGLSIVRRIIHWHDGRALIGKSKSLGGACFSLSWPRNQERR
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]