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The map label for this gene is dctA

Identifier: 77460457

GI number: 77460457

Start: 4777305

End: 4778639

Strand: Reverse

Name: dctA

Synonym: Pfl01_4236

Alternate gene names: 77460457

Gene position: 4778639-4777305 (Counterclockwise)

Preceding gene: 77460459

Following gene: 77460456

Centisome position: 74.22

GC content: 59.93

Gene sequence:

>1335_bases
ATGACGACTCGTCAGCCACTGTACAAATCCCTGTATTTCCAGGTGATCGTCGCAATCTGTATCGGTATTGCGCTCGGCCA
CTACTACCCGCAGTTCGGCGTGGCCGTAAAGCCGCTGGGTGACGGGTTCATCAAACTGATCAAAATGATCATCGCCCCGA
TCATCTTCTGCACCGTGGTCAGCGGCATCGCCGGCATGCAGAACATGAAATCGGTCGGCAAGACCGGCGGCTACGCACTC
CTGTACTTCGAAATCGTTTCGACCATCGCACTGCTGGTGGGCCTGATCGTGGTCAACATCGTGCAACCGGGCAACGGCAT
GCACATCGACGTCAGCACCCTCGACGCCTCGAAAGTCGCGACCTATGTTGCTCAAGGCGCAGACCAGAGCGTTGTCGGCT
TCCTGCTCAACGTGATCCCGACCACCATCGTCGGCGCGTTCGCCACCGGTGACATCCTGCAAGTGCTGATGTTCTCGGTG
ATCTTCGGTTTCGCCCTGCACCGCCTGGGTGCCTACGGCAAGCCGATCCTGGACTTCATCGATCGCTTCGCTCACGTGAT
GTTCAACATCATCAACATGATCATGAAGCTCGCGCCCATCGGTGCCTTCGGTGCGATGGCGTTCACCATCGGTGCCTACG
GTGTCGGCTCGCTGGTACAGCTGGGTCAACTGATGATCTGCTTCTACATCACTTGCCTGGCGTTCATCCTGATCGTGCTC
GGCGGTATCGCCCGCATGCACGGTTTCAGCGTGCTGAAAATGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGG
TACTTCCTCTTCGGAATCGGTACTGCCACGCATGCTGATCAAGATGGAGCGTCTGGGCGCGAAGAAATCGGTGGTAGGTC
TGGTGATCCCGACCGGCTACTCGTTCAACCTCGACGGCACCGCAATCTACCTGACCATGGCGGCCGTGTTCATCGCTCAG
GCGACCGACACCCACATGGACATCACTCACCAGATCACCCTGCTGGTGGTGTTGCTGCTGTCCTCCAAAGGCGCCGCCGG
TGTGACCGGTTCGGGCTTCATCGTGCTGGCGGCCACCCTGTCGGCGGTAGGTCACCTGCCGGTGGCCGGTCTGGCGCTGA
TCCTGGGTATCGACCGCTTCATGTCCGAAGCCCGTGCCCTGACCAACCTGGTCGGCAACGCCGTTGCCACCATCGTTGTG
GCCAAGTGGGTCAAGGAGCTGGACACCGACGTGCTGGACGCCGAGCTGGCCTCGGGCGGTCGCGGTATCGCCGATACCCG
TCCTGAAGATGACCTGGGCGTGGCCGAAGGCCCGACCCCGAGCAATGTGAAGTAA

Upstream 100 bases:

>100_bases
GTTCGACACAACAAATCCCTCCAGTGCAGGAGGGTTCGCAATTCAAGTGTCGCGTTCAACAGATGGATGTTGCCGCGCGG
GACTCTTGAGGAACTTTGCA

Downstream 100 bases:

>100_bases
GCATCGCTTGAATGGAAAAACCCGCTTCGGCGGGTTTTTTCTTGCCTGCTGTTTGAGCGCAACGGTCAGCACAACTGATG
CATCCGGTAATTGCCCCATC

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTTRQPLYKSLYFQVIVAICIGIALGHYYPQFGVAVKPLGDGFIKLIKMIIAPIIFCTVVSGIAGMQNMKSVGKTGGYAL
LYFEIVSTIALLVGLIVVNIVQPGNGMHIDVSTLDASKVATYVAQGADQSVVGFLLNVIPTTIVGAFATGDILQVLMFSV
IFGFALHRLGAYGKPILDFIDRFAHVMFNIINMIMKLAPIGAFGAMAFTIGAYGVGSLVQLGQLMICFYITCLAFILIVL
GGIARMHGFSVLKMIRYIREELLIVLGTSSSESVLPRMLIKMERLGAKKSVVGLVIPTGYSFNLDGTAIYLTMAAVFIAQ
ATDTHMDITHQITLLVVLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNAVATIVV
AKWVKELDTDVLDAELASGGRGIADTRPEDDLGVAEGPTPSNVK

Sequences:

>Translated_444_residues
MTTRQPLYKSLYFQVIVAICIGIALGHYYPQFGVAVKPLGDGFIKLIKMIIAPIIFCTVVSGIAGMQNMKSVGKTGGYAL
LYFEIVSTIALLVGLIVVNIVQPGNGMHIDVSTLDASKVATYVAQGADQSVVGFLLNVIPTTIVGAFATGDILQVLMFSV
IFGFALHRLGAYGKPILDFIDRFAHVMFNIINMIMKLAPIGAFGAMAFTIGAYGVGSLVQLGQLMICFYITCLAFILIVL
GGIARMHGFSVLKMIRYIREELLIVLGTSSSESVLPRMLIKMERLGAKKSVVGLVIPTGYSFNLDGTAIYLTMAAVFIAQ
ATDTHMDITHQITLLVVLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNAVATIVV
AKWVKELDTDVLDAELASGGRGIADTRPEDDLGVAEGPTPSNVK
>Mature_443_residues
TTRQPLYKSLYFQVIVAICIGIALGHYYPQFGVAVKPLGDGFIKLIKMIIAPIIFCTVVSGIAGMQNMKSVGKTGGYALL
YFEIVSTIALLVGLIVVNIVQPGNGMHIDVSTLDASKVATYVAQGADQSVVGFLLNVIPTTIVGAFATGDILQVLMFSVI
FGFALHRLGAYGKPILDFIDRFAHVMFNIINMIMKLAPIGAFGAMAFTIGAYGVGSLVQLGQLMICFYITCLAFILIVLG
GIARMHGFSVLKMIRYIREELLIVLGTSSSESVLPRMLIKMERLGAKKSVVGLVIPTGYSFNLDGTAIYLTMAAVFIAQA
TDTHMDITHQITLLVVLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNAVATIVVA
KWVKELDTDVLDAELASGGRGIADTRPEDDLGVAEGPTPSNVK

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI4827012, Length=475, Percent_Identity=24, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI40254478, Length=429, Percent_Identity=28.2051282051282, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI21314632, Length=406, Percent_Identity=26.6009852216749, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI5032093, Length=405, Percent_Identity=27.4074074074074, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI169790839, Length=458, Percent_Identity=25.1091703056769, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI223468566, Length=280, Percent_Identity=31.0714285714286, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI223468564, Length=282, Percent_Identity=31.5602836879433, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI66773030, Length=408, Percent_Identity=26.2254901960784, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI194239697, Length=442, Percent_Identity=24.4343891402715, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI262359914, Length=417, Percent_Identity=23.7410071942446, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI301601644, Length=150, Percent_Identity=31.3333333333333, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1789947, Length=410, Percent_Identity=76.0975609756098, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1790514, Length=411, Percent_Identity=40.8759124087591, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788024, Length=396, Percent_Identity=26.5151515151515, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789473, Length=326, Percent_Identity=22.6993865030675, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537407, Length=425, Percent_Identity=26.3529411764706, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71983106, Length=465, Percent_Identity=25.5913978494624, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71983099, Length=465, Percent_Identity=25.5913978494624, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71996953, Length=459, Percent_Identity=23.7472766884532, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=481, Percent_Identity=24.1164241164241, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=413, Percent_Identity=24.6973365617433, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=417, Percent_Identity=22.5419664268585, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24583025, Length=419, Percent_Identity=24.582338902148, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137668, Length=419, Percent_Identity=24.582338902148, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24583023, Length=419, Percent_Identity=24.582338902148, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281360483, Length=422, Percent_Identity=23.2227488151659, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137666, Length=422, Percent_Identity=23.2227488151659, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281360481, Length=422, Percent_Identity=23.2227488151659, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_PSEPF (Q3K8D1)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_349964.1
- ProteinModelPortal:   Q3K8D1
- SMR:   Q3K8D1
- GeneID:   3717814
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4236
- eggNOG:   COG1301
- HOGENOM:   HBG739804
- OMA:   ACFYITC
- ProtClustDB:   PRK01663
- BioCyc:   PFLU205922:PFL_4236-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 47037; Mature: 46906

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13601fd0)-; HASH(0x13d81ce0)-; HASH(0x137afa94)-; HASH(0x13d9c92c)-; HASH(0x13ea6a90)-; HASH(0x13dfc140)-; HASH(0x13abdad8)-; HASH(0x13b391dc)-; HASH(0x1468b05c)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRQPLYKSLYFQVIVAICIGIALGHYYPQFGVAVKPLGDGFIKLIKMIIAPIIFCTVV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHH
SGIAGMQNMKSVGKTGGYALLYFEIVSTIALLVGLIVVNIVQPGNGMHIDVSTLDASKVA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
TYVAQGADQSVVGFLLNVIPTTIVGAFATGDILQVLMFSVIFGFALHRLGAYGKPILDFI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DRFAHVMFNIINMIMKLAPIGAFGAMAFTIGAYGVGSLVQLGQLMICFYITCLAFILIVL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGIARMHGFSVLKMIRYIREELLIVLGTSSSESVLPRMLIKMERLGAKKSVVGLVIPTGY
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCE
SFNLDGTAIYLTMAAVFIAQATDTHMDITHQITLLVVLLLSSKGAAGVTGSGFIVLAATL
EECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
SAVGHLPVAGLALILGIDRFMSEARALTNLVGNAVATIVVAKWVKELDTDVLDAELASGG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RGIADTRPEDDLGVAEGPTPSNVK
CCCCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure 
TTRQPLYKSLYFQVIVAICIGIALGHYYPQFGVAVKPLGDGFIKLIKMIIAPIIFCTVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHH
SGIAGMQNMKSVGKTGGYALLYFEIVSTIALLVGLIVVNIVQPGNGMHIDVSTLDASKVA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
TYVAQGADQSVVGFLLNVIPTTIVGAFATGDILQVLMFSVIFGFALHRLGAYGKPILDFI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
DRFAHVMFNIINMIMKLAPIGAFGAMAFTIGAYGVGSLVQLGQLMICFYITCLAFILIVL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGIARMHGFSVLKMIRYIREELLIVLGTSSSESVLPRMLIKMERLGAKKSVVGLVIPTGY
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCE
SFNLDGTAIYLTMAAVFIAQATDTHMDITHQITLLVVLLLSSKGAAGVTGSGFIVLAATL
EECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
SAVGHLPVAGLALILGIDRFMSEARALTNLVGNAVATIVVAKWVKELDTDVLDAELASGG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RGIADTRPEDDLGVAEGPTPSNVK
CCCCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA