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The map label for this gene is ttcA [H]

Identifier: 77460453

GI number: 77460453

Start: 4773608

End: 4774432

Strand: Reverse

Name: ttcA [H]

Synonym: Pfl01_4232

Alternate gene names: 77460453

Gene position: 4774432-4773608 (Counterclockwise)

Preceding gene: 77460456

Following gene: 77460452

Centisome position: 74.16

GC content: 59.52

Gene sequence:

>825_bases
ATGGGAACTCTTACGGTCAACCAGAACAAACTGCAAAAGCGCCTGCGCCGCCTGGCCGGCGAAGCCGTCACCGACTTCAA
CATGATCGAAGACGGCGACAAGGTCATGGTCTGCCTGTCCGGGGGCAAGGACAGCTACACCATGCTCGACGTGCTGATGC
ACCTGCAGAAGGTTGCACCGATCAAGTTCGAGATCGTCGCCGTGAACATGGACCAGAAGCAGCCGGGTTTCCCCGAGCAC
GTGCTGCCGGCTTACCTGAAAGAGCTGGGCATCGAGTACCACATCGTCGAGAAGGACACCTACTCGGTGGTCAAGGAGCT
GATCCCGGAAGGCAAGACCACCTGCTCGCTGTGCTCGCGCCTGCGTCGCGGCACGCTGTACACCTTCGCCGACGAAATCG
GCGCGACCAAAATGGCCCTCGGTCATCATCGCGACGACATCGTCGAGACGTTCTTCCTCAACATGTTCTTCAACGGCAGC
CTCAAGGCCATGCCGCCGAAGCTGCGCGCCGATGACGGGCGCAACGTGGTGATCCGTCCGCTGGCCTACTGCAACGAGAA
AGACATCCAGGCCTACTCGGACTTCAAGCAATTCCCGATCATCCCGTGCAACCTCTGCGGTTCCCAGGAAAACCTGCAAC
GCCAGGTGGTCAAGGAAATGCTCCAGGACTGGGAACGCAAGACCCCGGGCCGCACCGAAAGCATCTTCCGCAGCCTGCAG
AACGTGATCCCGTCGCAACTGGCTGACCGTAACCTGTTTGACTTCACCAGCCTGAAGATCGACGAGACGGCGGCTTCGCG
TTTCGTCAATGTGGTGAACCTGTAA

Upstream 100 bases:

>100_bases
TGTGCAAACGGGCATTCGAGGGGGCGACAACCTGTGGCCACAAGCCAGGTGTTTTTGCGTAAAATGCCCGCCTTTCGTCA
CACCTCACGGATTTCGCGTC

Downstream 100 bases:

>100_bases
GTGATCTGATCGCTCGAAAGAAACGGCGCTCGCGGGCGCCGTTTTCAATTCATTTTTTAGGAGAGGGCATGCGCGACTAC
AAGTGGCTGCACGAATACTG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MGTLTVNQNKLQKRLRRLAGEAVTDFNMIEDGDKVMVCLSGGKDSYTMLDVLMHLQKVAPIKFEIVAVNMDQKQPGFPEH
VLPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSRLRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGS
LKAMPPKLRADDGRNVVIRPLAYCNEKDIQAYSDFKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRSLQ
NVIPSQLADRNLFDFTSLKIDETAASRFVNVVNL

Sequences:

>Translated_274_residues
MGTLTVNQNKLQKRLRRLAGEAVTDFNMIEDGDKVMVCLSGGKDSYTMLDVLMHLQKVAPIKFEIVAVNMDQKQPGFPEH
VLPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSRLRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGS
LKAMPPKLRADDGRNVVIRPLAYCNEKDIQAYSDFKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRSLQ
NVIPSQLADRNLFDFTSLKIDETAASRFVNVVNL
>Mature_273_residues
GTLTVNQNKLQKRLRRLAGEAVTDFNMIEDGDKVMVCLSGGKDSYTMLDVLMHLQKVAPIKFEIVAVNMDQKQPGFPEHV
LPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSRLRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGSL
KAMPPKLRADDGRNVVIRPLAYCNEKDIQAYSDFKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRSLQN
VIPSQLADRNLFDFTSLKIDETAASRFVNVVNL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=264, Percent_Identity=68.5606060606061, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI193211090, Length=234, Percent_Identity=34.6153846153846, Blast_Score=134, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17540090, Length=231, Percent_Identity=26.8398268398268, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24651972, Length=218, Percent_Identity=26.605504587156, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 31171; Mature: 31040

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLTVNQNKLQKRLRRLAGEAVTDFNMIEDGDKVMVCLSGGKDSYTMLDVLMHLQKVAP
CCCEECCHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
IKFEIVAVNMDQKQPGFPEHVLPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSR
CEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHH
LRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGSLKAMPPKLRADDGRNVVIRP
HHCCCEEEHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEH
LAYCNEKDIQAYSDFKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRSLQ
HHHCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NVIPSQLADRNLFDFTSLKIDETAASRFVNVVNL
HHHHHHHHCCCCHHHHHCEECHHHHHHHHHHHCC
>Mature Secondary Structure 
GTLTVNQNKLQKRLRRLAGEAVTDFNMIEDGDKVMVCLSGGKDSYTMLDVLMHLQKVAP
CCEECCHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
IKFEIVAVNMDQKQPGFPEHVLPAYLKELGIEYHIVEKDTYSVVKELIPEGKTTCSLCSR
CEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHH
LRRGTLYTFADEIGATKMALGHHRDDIVETFFLNMFFNGSLKAMPPKLRADDGRNVVIRP
HHCCCEEEHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEH
LAYCNEKDIQAYSDFKQFPIIPCNLCGSQENLQRQVVKEMLQDWERKTPGRTESIFRSLQ
HHHCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NVIPSQLADRNLFDFTSLKIDETAASRFVNVVNL
HHHHHHHHCCCCHHHHHCEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA