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The map label for this gene is btuB [H]

Identifier: 77460430

GI number: 77460430

Start: 4749484

End: 4751607

Strand: Direct

Name: btuB [H]

Synonym: Pfl01_4209

Alternate gene names: 77460430

Gene position: 4749484-4751607 (Clockwise)

Preceding gene: 77460420

Following gene: 77460431

Centisome position: 73.77

GC content: 63.18

Gene sequence:

>2124_bases
GTGTTTTCTGGCCCCCCTTCCCCGCGTTCGTCCCTGCTGCTGGCGCTGCTGTTCAGCCCGGTGCTGCTGGCGGACGACCT
GTTTGTCGACAGTGAGGCCTTGCCGCAGATCCTGACCGCCACGCGTTTGAAACAGTCGCCAGCGGAAGTCCCCGGCAGCA
TGACCGTGCTCGACAGCGAACTGATCAGCGCCAGTGGCGCCCGGGACATCAGTGAACTGCTGCGGCTGGTGCCGGGGATG
ATGGTCGGCAACATCAGCGGCAATCAGGCCGTGGTGAATTATCACGGAACCAACGCCACTGAAGCCCGGCGCATGCAAGT
GCTGATCGACGGCCGCTCGGTGTACCGCGCCGGCCTCGCGACGGTGGACTGGAGCGACATTCCGGTGGCCATGGAAGATA
TCGAGCGCATCGAAGTCTTTCGCGGCCCGAACACCGTCAGCTACGGCGCCAACGCGCTGATGGCAGTGGTCAACATCATC
ACCCGTAACCCGGCGGACAGCCACGGCACGCGGCTGAAGCTGACGCGCGGCCAACGCGGCATCAACGACTTCTACGCCAG
TCAGAGTACCGGCTGGAATGGCGGCGATCTGCGTCTGTCGCTGTCCGGCCAGGAAGACGACGGCTTCGACAGCGATCGCA
CTGGCGCCGATTACCGCGACAACCGCCGCTTGAACCGCTTCAGTCTCGCCGTCAGCCAGACCCTGAGCGACAACCAGAGC
CTGGACTGGCAGATCAACGCCAAGGACGGGACCAATCAGCGGCCTTACACCTACCGACCGGTGTTCTCCGGGATTACCGC
TGCCGGGAACAATTCCGACGTGGTTGCCAAGGACTATGCCGGTTCGGTGCGCTGGAATCTGGACCTCAATCCCGATCACA
GCCTTTATGTGCAAGGTTCTGCGCAACACTGGGATCGCCAGCAAACCTGGCGCGCCTGTGACGCCGAAGTGTCGTTCAGC
CCGCAATTGACGGAGCTGTGGCAACTCAACCCGAACTACACCGAGCGCTTGGCGCGCAACATGACGCTGTTCACCGGCCC
CGGCGCACCCGCCGGTACACCGCAGGAAATGGCCCTGGCCAATCAAGTGCTGGACCAATGGCGCAACGGCGCTCGCCAGA
CCCTGTGCGGCGACATCGACCAGAGTGCCCGGGAATCGCGCTACGACCTGGAATTGCAGGACACCCTCAGCCTGTCCGAT
AGCCTGCGTCTGGTCAGCGGCCTGAACTATCGTTACGACCGGGCCGATTCCGAGACCTATTTCAACGGCACGCTCGATGA
CACCACGTGGCGCGCCTTTGGCCAACTGGAGTGGCGCGCCAGCGAACACTGGTTGTTACAGGGCGGCGCCATGTTCGAAG
ACATGCAATTGATCGGCAGCTCACTGACGCCCCGGTTTGCGATCAACTACCTGTTCAACCCGCGCCACAGCCTGCGAGCG
GTGTACTCGGAAGCCATCCGTTCGCCGGACATGTTCGAGAACAACGTCAACTGGAGTTATCAGGTGACCAACCTGCGGCC
CGCCGCCTATGGCCAGTCTTCGGCCCGCTACTTCGTCAAGACCCGGGGCCCCGGCGATCTGGACCAGGAGCACATGCGCT
CGCGGGAACTGGGCTACAACGGCTATTTCCCGGAGTGGGGCCTGGCGCTGGATGTGAAGCTGTTCTACGACGAAATCACC
GGGATGATCAGCGAGCCGCTGCGCAACAATCAGTACATCGCCAGCAACGCCAACGACTCGCGCTTTCGCGGCACCGAAAC
GCAACTGGACTGGCGCCTGAGTGCGGCCGACCGCTTACGCCTGACCTACGCCTTCGTCGACGCCGAGGCGAGCAACCCGC
TGGATCAGCAATTCACCGCACGCAACAGCGGTTCGGCGGGCTGGCTGCGCGATTGGGGCCACGGCTGGAACAGCGCCCTC
TTCTACTACGGTGACAACGCGCTCAACGGTTACCGCTTCGAGCGGGTCGACACGCGGATCGCCAAACGCATCGCCCTGGG
CAAGGCCCAGCTGCAACTGGCCGGCGTGCTGCAACAACGCCTCGACCATGAGCCGACCACGTTCGTCGACAACAATTACG
ACGAACGCCGCGTGGTGTATTTCAGCGCCGAGCTGGAGTTCTAG

Upstream 100 bases:

>100_bases
AGAAGACCAATAAAACCGAGGAAAATGCCTGCAACGTGACTGGAAAAGTCGTCGACCTCGGTTAACATGCGCGCCAATTC
TTTGCTATCAAGGCTGCGTC

Downstream 100 bases:

>100_bases
ATGCGTTACGCCATGTCACGGAAGATGCCAACGCTCGGCCAATGGCTGGCCGGGCTGTGTCTGTTCCTGACGGCGTGGCT
GCATGGCGTGGCGGTGCAGG

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MFSGPPSPRSSLLLALLFSPVLLADDLFVDSEALPQILTATRLKQSPAEVPGSMTVLDSELISASGARDISELLRLVPGM
MVGNISGNQAVVNYHGTNATEARRMQVLIDGRSVYRAGLATVDWSDIPVAMEDIERIEVFRGPNTVSYGANALMAVVNII
TRNPADSHGTRLKLTRGQRGINDFYASQSTGWNGGDLRLSLSGQEDDGFDSDRTGADYRDNRRLNRFSLAVSQTLSDNQS
LDWQINAKDGTNQRPYTYRPVFSGITAAGNNSDVVAKDYAGSVRWNLDLNPDHSLYVQGSAQHWDRQQTWRACDAEVSFS
PQLTELWQLNPNYTERLARNMTLFTGPGAPAGTPQEMALANQVLDQWRNGARQTLCGDIDQSARESRYDLELQDTLSLSD
SLRLVSGLNYRYDRADSETYFNGTLDDTTWRAFGQLEWRASEHWLLQGGAMFEDMQLIGSSLTPRFAINYLFNPRHSLRA
VYSEAIRSPDMFENNVNWSYQVTNLRPAAYGQSSARYFVKTRGPGDLDQEHMRSRELGYNGYFPEWGLALDVKLFYDEIT
GMISEPLRNNQYIASNANDSRFRGTETQLDWRLSAADRLRLTYAFVDAEASNPLDQQFTARNSGSAGWLRDWGHGWNSAL
FYYGDNALNGYRFERVDTRIAKRIALGKAQLQLAGVLQQRLDHEPTTFVDNNYDERRVVYFSAELEF

Sequences:

>Translated_707_residues
MFSGPPSPRSSLLLALLFSPVLLADDLFVDSEALPQILTATRLKQSPAEVPGSMTVLDSELISASGARDISELLRLVPGM
MVGNISGNQAVVNYHGTNATEARRMQVLIDGRSVYRAGLATVDWSDIPVAMEDIERIEVFRGPNTVSYGANALMAVVNII
TRNPADSHGTRLKLTRGQRGINDFYASQSTGWNGGDLRLSLSGQEDDGFDSDRTGADYRDNRRLNRFSLAVSQTLSDNQS
LDWQINAKDGTNQRPYTYRPVFSGITAAGNNSDVVAKDYAGSVRWNLDLNPDHSLYVQGSAQHWDRQQTWRACDAEVSFS
PQLTELWQLNPNYTERLARNMTLFTGPGAPAGTPQEMALANQVLDQWRNGARQTLCGDIDQSARESRYDLELQDTLSLSD
SLRLVSGLNYRYDRADSETYFNGTLDDTTWRAFGQLEWRASEHWLLQGGAMFEDMQLIGSSLTPRFAINYLFNPRHSLRA
VYSEAIRSPDMFENNVNWSYQVTNLRPAAYGQSSARYFVKTRGPGDLDQEHMRSRELGYNGYFPEWGLALDVKLFYDEIT
GMISEPLRNNQYIASNANDSRFRGTETQLDWRLSAADRLRLTYAFVDAEASNPLDQQFTARNSGSAGWLRDWGHGWNSAL
FYYGDNALNGYRFERVDTRIAKRIALGKAQLQLAGVLQQRLDHEPTTFVDNNYDERRVVYFSAELEF
>Mature_707_residues
MFSGPPSPRSSLLLALLFSPVLLADDLFVDSEALPQILTATRLKQSPAEVPGSMTVLDSELISASGARDISELLRLVPGM
MVGNISGNQAVVNYHGTNATEARRMQVLIDGRSVYRAGLATVDWSDIPVAMEDIERIEVFRGPNTVSYGANALMAVVNII
TRNPADSHGTRLKLTRGQRGINDFYASQSTGWNGGDLRLSLSGQEDDGFDSDRTGADYRDNRRLNRFSLAVSQTLSDNQS
LDWQINAKDGTNQRPYTYRPVFSGITAAGNNSDVVAKDYAGSVRWNLDLNPDHSLYVQGSAQHWDRQQTWRACDAEVSFS
PQLTELWQLNPNYTERLARNMTLFTGPGAPAGTPQEMALANQVLDQWRNGARQTLCGDIDQSARESRYDLELQDTLSLSD
SLRLVSGLNYRYDRADSETYFNGTLDDTTWRAFGQLEWRASEHWLLQGGAMFEDMQLIGSSLTPRFAINYLFNPRHSLRA
VYSEAIRSPDMFENNVNWSYQVTNLRPAAYGQSSARYFVKTRGPGDLDQEHMRSRELGYNGYFPEWGLALDVKLFYDEIT
GMISEPLRNNQYIASNANDSRFRGTETQLDWRLSAADRLRLTYAFVDAEASNPLDQQFTARNSGSAGWLRDWGHGWNSAL
FYYGDNALNGYRFERVDTRIAKRIALGKAQLQLAGVLQQRLDHEPTTFVDNNYDERRVVYFSAELEF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=170, Percent_Identity=32.9411764705882, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786798, Length=546, Percent_Identity=23.2600732600733, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790405, Length=151, Percent_Identity=31.1258278145695, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 79520; Mature: 79520

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGPPSPRSSLLLALLFSPVLLADDLFVDSEALPQILTATRLKQSPAEVPGSMTVLDSE
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHCCHHHCCCCEEEEHHH
LISASGARDISELLRLVPGMMVGNISGNQAVVNYHGTNATEARRMQVLIDGRSVYRAGLA
HHHCCCCHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCEEEEEEECCCHHHHCCCC
TVDWSDIPVAMEDIERIEVFRGPNTVSYGANALMAVVNIITRNPADSHGTRLKLTRGQRG
EECCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
INDFYASQSTGWNGGDLRLSLSGQEDDGFDSDRTGADYRDNRRLNRFSLAVSQTLSDNQS
CHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
LDWQINAKDGTNQRPYTYRPVFSGITAAGNNSDVVAKDYAGSVRWNLDLNPDHSLYVQGS
CEEEEECCCCCCCCCEEECHHHCCEEECCCCCCEEEECCCCCEEEEEECCCCCEEEEECC
AQHWDRQQTWRACDAEVSFSPQLTELWQLNPNYTERLARNMTLFTGPGAPAGTPQEMALA
HHHCCHHHHHHHCCCCCCCCCCHHHHEECCCCHHHHHHCCCEEEECCCCCCCCHHHHHHH
NQVLDQWRNGARQTLCGDIDQSARESRYDLELQDTLSLSDSLRLVSGLNYRYDRADSETY
HHHHHHHHCCCHHHHHCCCHHHHHHCCCCEEEHHHHCHHHHHHHHHCCCCEECCCCCCEE
FNGTLDDTTWRAFGQLEWRASEHWLLQGGAMFEDMQLIGSSLTPRFAINYLFNPRHSLRA
ECCCCCCCHHHHHCEEEEECCCEEEEECCCHHHHHHHHCCCCCCCEEEEEEECCHHHHHH
VYSEAIRSPDMFENNVNWSYQVTNLRPAAYGQSSARYFVKTRGPGDLDQEHMRSRELGYN
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCC
GYFPEWGLALDVKLFYDEITGMISEPLRNNQYIASNANDSRFRGTETQLDWRLSAADRLR
CCCCCCCCEEEEEEHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCEE
LTYAFVDAEASNPLDQQFTARNSGSAGWLRDWGHGWNSALFYYGDNALNGYRFERVDTRI
EEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCEEHHHHHHHH
AKRIALGKAQLQLAGVLQQRLDHEPTTFVDNNYDERRVVYFSAELEF
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MFSGPPSPRSSLLLALLFSPVLLADDLFVDSEALPQILTATRLKQSPAEVPGSMTVLDSE
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHCCHHHCCCCEEEEHHH
LISASGARDISELLRLVPGMMVGNISGNQAVVNYHGTNATEARRMQVLIDGRSVYRAGLA
HHHCCCCHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCCEEEEEEECCCHHHHCCCC
TVDWSDIPVAMEDIERIEVFRGPNTVSYGANALMAVVNIITRNPADSHGTRLKLTRGQRG
EECCCCCCHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
INDFYASQSTGWNGGDLRLSLSGQEDDGFDSDRTGADYRDNRRLNRFSLAVSQTLSDNQS
CHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
LDWQINAKDGTNQRPYTYRPVFSGITAAGNNSDVVAKDYAGSVRWNLDLNPDHSLYVQGS
CEEEEECCCCCCCCCEEECHHHCCEEECCCCCCEEEECCCCCEEEEEECCCCCEEEEECC
AQHWDRQQTWRACDAEVSFSPQLTELWQLNPNYTERLARNMTLFTGPGAPAGTPQEMALA
HHHCCHHHHHHHCCCCCCCCCCHHHHEECCCCHHHHHHCCCEEEECCCCCCCCHHHHHHH
NQVLDQWRNGARQTLCGDIDQSARESRYDLELQDTLSLSDSLRLVSGLNYRYDRADSETY
HHHHHHHHCCCHHHHHCCCHHHHHHCCCCEEEHHHHCHHHHHHHHHCCCCEECCCCCCEE
FNGTLDDTTWRAFGQLEWRASEHWLLQGGAMFEDMQLIGSSLTPRFAINYLFNPRHSLRA
ECCCCCCCHHHHHCEEEEECCCEEEEECCCHHHHHHHHCCCCCCCEEEEEEECCHHHHHH
VYSEAIRSPDMFENNVNWSYQVTNLRPAAYGQSSARYFVKTRGPGDLDQEHMRSRELGYN
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCC
GYFPEWGLALDVKLFYDEITGMISEPLRNNQYIASNANDSRFRGTETQLDWRLSAADRLR
CCCCCCCCEEEEEEHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCEE
LTYAFVDAEASNPLDQQFTARNSGSAGWLRDWGHGWNSALFYYGDNALNGYRFERVDTRI
EEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCCCCEEEEECCCCCCCEEHHHHHHHH
AKRIALGKAQLQLAGVLQQRLDHEPTTFVDNNYDERRVVYFSAELEF
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA