Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 77460279

GI number: 77460279

Start: 4583146

End: 4584222

Strand: Reverse

Name: rfbB [H]

Synonym: Pfl01_4058

Alternate gene names: 77460279

Gene position: 4584222-4583146 (Counterclockwise)

Preceding gene: 77460280

Following gene: 77460278

Centisome position: 71.2

GC content: 55.62

Gene sequence:

>1077_bases
GTGAAAATTCTAGTCACCGGCGGTGCAGGTTTTATCGGTTCGGCAGTCATCCGACACATCATTTCCAATACCACCGACTC
CGTTGTCAATGTCGATAAGCTCACCTATGCCGGCAACCTTGAGTCGCTCGCCGAAGTCAGCCAGGATTCGCGTTACGCAT
TCGAGCGAGTCGATATCTGTGATCGTGATCAGATCGACCGTGTTCTGCGTGAACATCAACCAGATGCCATCATGCACCTG
GCTGCCGAATCCCACGTCGATCGTTCCATTTCCGGCCCGTCCGAGTTTATCCAGACCAATATCATTGGCACCTACACTCT
TCTCGAAGCGGCCCGCCACTACTGGGCGGCCCTCGATGATCAACGCAAGGCCGCTTTCCGTTTTCACCATATCTCCACCG
ACGAAGTTTACGGAGACCTTGAGGGTCCCGAAGATCTCTTCACGGAAACCACGCCGTATCAGCCAAGTTCGCCTTACTCG
GCCAGCAAGGCCAGCTCCGACCACCTGGTTCGCGCCTGGGCTCGTACCTATGGCCTGCCAACGCTCGTTACCAACTGCTC
GAACAACTATGGTCCTTGCCACTTCCCCGAGAAGCTGATCCCGCTGATCATTCTCAACGCGCTGGAAGGCAAGCCGCTGC
CTGTTTACGGCAAGGGTAACCAGGTGCGTGACTGGCTTTATGTCGAGGATCACGCACGCGCCCTGTACAAGGTCGTGACC
GAGGGCGTTATCGGCGAGACCTATAACATTGGCGGCCACAACGAAAAGCAGAACATCGAAGTGGTGAATACCCTTTGCGC
CCTGCTTGACGAACTGCGTCCTGACTCCGCTCACCGTCCGCATGCCAGCCTGATCACCTATGTTCAGGATCGTCCGGGAC
ACGATCAGCGTTACGCGATTGACGCCAGTAAAATCCAGCGTGAGCTGGGCTGGACGCCCGAGGAGACCTTTGAAAGTGGA
ATCCGTAAAACGGTCGAGTGGTATTTGAGCAATACTGAGTGGGTCGCTCACGTGAAGAGTGGCAGCTACCAACAGTGGAT
TGACCAGAACTACGCAGGTCGTTCGACAAAGGCATGA

Upstream 100 bases:

>100_bases
TTTCTATCGAAAGCCTCCGCAAAGTTGATGGCTTTTGCATTCATGGATCAGGCAGGGCCCGGTTGGCGGTCCTCTCAGTA
ACTCAAGGGTATGGTTTCGC

Downstream 100 bases:

>100_bases
GAATCCTCCTTCTAGGAAAAAATGGCCAGGTGGGCTGGGAGCTGCAACGCTCCCTGGCGCCACTGGGCGAGCTGTTGTCC
CTTGATCGTCAAACCGTGGG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKILVTGGAGFIGSAVIRHIISNTTDSVVNVDKLTYAGNLESLAEVSQDSRYAFERVDICDRDQIDRVLREHQPDAIMHL
AAESHVDRSISGPSEFIQTNIIGTYTLLEAARHYWAALDDQRKAAFRFHHISTDEVYGDLEGPEDLFTETTPYQPSSPYS
ASKASSDHLVRAWARTYGLPTLVTNCSNNYGPCHFPEKLIPLIILNALEGKPLPVYGKGNQVRDWLYVEDHARALYKVVT
EGVIGETYNIGGHNEKQNIEVVNTLCALLDELRPDSAHRPHASLITYVQDRPGHDQRYAIDASKIQRELGWTPEETFESG
IRKTVEWYLSNTEWVAHVKSGSYQQWIDQNYAGRSTKA

Sequences:

>Translated_358_residues
MKILVTGGAGFIGSAVIRHIISNTTDSVVNVDKLTYAGNLESLAEVSQDSRYAFERVDICDRDQIDRVLREHQPDAIMHL
AAESHVDRSISGPSEFIQTNIIGTYTLLEAARHYWAALDDQRKAAFRFHHISTDEVYGDLEGPEDLFTETTPYQPSSPYS
ASKASSDHLVRAWARTYGLPTLVTNCSNNYGPCHFPEKLIPLIILNALEGKPLPVYGKGNQVRDWLYVEDHARALYKVVT
EGVIGETYNIGGHNEKQNIEVVNTLCALLDELRPDSAHRPHASLITYVQDRPGHDQRYAIDASKIQRELGWTPEETFESG
IRKTVEWYLSNTEWVAHVKSGSYQQWIDQNYAGRSTKA
>Mature_358_residues
MKILVTGGAGFIGSAVIRHIISNTTDSVVNVDKLTYAGNLESLAEVSQDSRYAFERVDICDRDQIDRVLREHQPDAIMHL
AAESHVDRSISGPSEFIQTNIIGTYTLLEAARHYWAALDDQRKAAFRFHHISTDEVYGDLEGPEDLFTETTPYQPSSPYS
ASKASSDHLVRAWARTYGLPTLVTNCSNNYGPCHFPEKLIPLIILNALEGKPLPVYGKGNQVRDWLYVEDHARALYKVVT
EGVIGETYNIGGHNEKQNIEVVNTLCALLDELRPDSAHRPHASLITYVQDRPGHDQRYAIDASKIQRELGWTPEETFESG
IRKTVEWYLSNTEWVAHVKSGSYQQWIDQNYAGRSTKA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=331, Percent_Identity=35.9516616314199, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI42516563, Length=341, Percent_Identity=26.099706744868, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=75.1381215469613, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=73.6231884057971, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1788366, Length=377, Percent_Identity=25.1989389920424, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=25.3561253561254, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=37.5, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI115532424, Length=327, Percent_Identity=33.6391437308869, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71982035, Length=369, Percent_Identity=27.10027100271, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=371, Percent_Identity=26.9541778975741, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=23.7951807228916, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319493, Length=362, Percent_Identity=25.414364640884, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19923002, Length=372, Percent_Identity=27.9569892473118, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21356223, Length=335, Percent_Identity=25.0746268656716, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40275; Mature: 40275

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVIRHIISNTTDSVVNVDKLTYAGNLESLAEVSQDSRYAFERVDIC
CEEEEECCCHHHHHHHHHHHHCCCCCHHEEHHHHEECCCHHHHHHHHHHHHHHHHHCCCC
DRDQIDRVLREHQPDAIMHLAAESHVDRSISGPSEFIQTNIIGTYTLLEAARHYWAALDD
CHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
QRKAAFRFHHISTDEVYGDLEGPEDLFTETTPYQPSSPYSASKASSDHLVRAWARTYGLP
HHHHHHHEECCCHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
TLVTNCSNNYGPCHFPEKLIPLIILNALEGKPLPVYGKGNQVRDWLYVEDHARALYKVVT
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEHHHHHHHHHHHH
EGVIGETYNIGGHNEKQNIEVVNTLCALLDELRPDSAHRPHASLITYVQDRPGHDQRYAI
HHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEE
DASKIQRELGWTPEETFESGIRKTVEWYLSNTEWVAHVKSGSYQQWIDQNYAGRSTKA
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVIRHIISNTTDSVVNVDKLTYAGNLESLAEVSQDSRYAFERVDIC
CEEEEECCCHHHHHHHHHHHHCCCCCHHEEHHHHEECCCHHHHHHHHHHHHHHHHHCCCC
DRDQIDRVLREHQPDAIMHLAAESHVDRSISGPSEFIQTNIIGTYTLLEAARHYWAALDD
CHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
QRKAAFRFHHISTDEVYGDLEGPEDLFTETTPYQPSSPYSASKASSDHLVRAWARTYGLP
HHHHHHHEECCCHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
TLVTNCSNNYGPCHFPEKLIPLIILNALEGKPLPVYGKGNQVRDWLYVEDHARALYKVVT
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEHHHHHHHHHHHH
EGVIGETYNIGGHNEKQNIEVVNTLCALLDELRPDSAHRPHASLITYVQDRPGHDQRYAI
HHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEE
DASKIQRELGWTPEETFESGIRKTVEWYLSNTEWVAHVKSGSYQQWIDQNYAGRSTKA
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]