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The map label for this gene is rfbD [H]

Identifier: 77460278

GI number: 77460278

Start: 4582256

End: 4583149

Strand: Reverse

Name: rfbD [H]

Synonym: Pfl01_4057

Alternate gene names: 77460278

Gene position: 4583149-4582256 (Counterclockwise)

Preceding gene: 77460279

Following gene: 77460277

Centisome position: 71.18

GC content: 57.49

Gene sequence:

>894_bases
ATGAGAATCCTCCTTCTAGGAAAAAATGGCCAGGTGGGCTGGGAGCTGCAACGCTCCCTGGCGCCACTGGGCGAGCTGTT
GTCCCTTGATCGTCAAACCGTGGGCGGTTTGTGTGGTGATCTCACTGATCTCGAAGGTCTGCGCGCGACCATTCGCACGG
TCAGGCCAGACGTGATCGTTAATGCCGCCGCCTACACTGCCGTAGACAAAGCCGAATCCGAGCGGGAGCTGGTTGATCTG
GTGAACGTCAAGGCGGTGCAGGCCTTGGCCGAGGAGGCATTATCCCTGGGCGCATGGCTGGTTCACTATTCAACGGACTA
CGTTTTCAGCGGAGAGGGCACTACCCCCTGGCTGGAAGACGCTGCAGTTGCACCGGTCAATCACTACGGTGCCAGCAAGT
TTGCCGGGGAAGAGGCGGTCGTTGCATCCGGCTGCAAGTACCTGATCTTTCGTCCAAGCTGGATCTACAGTGCTCGCGGC
AACAACTTCGCCAAGACCATGCTTCGCCTGGCCAGAGAGCGTGAAGAACTGAATGTGATCAACGATCAGATCGGTGCGCC
GACCGGTGCGGATCTGATCGCCGATGTCACTGCACACGCCATTCGCCAGGTTTTGCAGCGCCCGGATCTGGCAGGTGTCT
ACCACCTGGCGGCGGCGGGTGAGGTGTCATGGCATGGCTATGCCCAACATGTTGTCGAAGTGGCGAAAGCGCATGGCGAG
ACGCTGGCTGTGATGTCGATCAATCCGATTGAAACGGCAGCCTATTCGACGCCTGCGCGTCGTCCCCGGAATTCGCGATT
GAATACCCGGAAGCTGCGTGACAATTTTTCTCTACACTTGCCGGATTGGCAAAGTGGTGTAACCCGAATGCTTATGGAAG
TTCTGAATAAATGA

Upstream 100 bases:

>100_bases
TCGAGTGGTATTTGAGCAATACTGAGTGGGTCGCTCACGTGAAGAGTGGCAGCTACCAACAGTGGATTGACCAGAACTAC
GCAGGTCGTTCGACAAAGGC

Downstream 100 bases:

>100_bases
CCACGACAAATCGTAAAGGCATCATCCTCGCTGGCGGCTCGGGCACTCGTCTGCATCCGTTGACCCTTGGTGTTTCCAAG
CAGATGTTGCCGATTTACGA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRILLLGKNGQVGWELQRSLAPLGELLSLDRQTVGGLCGDLTDLEGLRATIRTVRPDVIVNAAAYTAVDKAESERELVDL
VNVKAVQALAEEALSLGAWLVHYSTDYVFSGEGTTPWLEDAAVAPVNHYGASKFAGEEAVVASGCKYLIFRPSWIYSARG
NNFAKTMLRLAREREELNVINDQIGAPTGADLIADVTAHAIRQVLQRPDLAGVYHLAAAGEVSWHGYAQHVVEVAKAHGE
TLAVMSINPIETAAYSTPARRPRNSRLNTRKLRDNFSLHLPDWQSGVTRMLMEVLNK

Sequences:

>Translated_297_residues
MRILLLGKNGQVGWELQRSLAPLGELLSLDRQTVGGLCGDLTDLEGLRATIRTVRPDVIVNAAAYTAVDKAESERELVDL
VNVKAVQALAEEALSLGAWLVHYSTDYVFSGEGTTPWLEDAAVAPVNHYGASKFAGEEAVVASGCKYLIFRPSWIYSARG
NNFAKTMLRLAREREELNVINDQIGAPTGADLIADVTAHAIRQVLQRPDLAGVYHLAAAGEVSWHGYAQHVVEVAKAHGE
TLAVMSINPIETAAYSTPARRPRNSRLNTRKLRDNFSLHLPDWQSGVTRMLMEVLNK
>Mature_297_residues
MRILLLGKNGQVGWELQRSLAPLGELLSLDRQTVGGLCGDLTDLEGLRATIRTVRPDVIVNAAAYTAVDKAESERELVDL
VNVKAVQALAEEALSLGAWLVHYSTDYVFSGEGTTPWLEDAAVAPVNHYGASKFAGEEAVVASGCKYLIFRPSWIYSARG
NNFAKTMLRLAREREELNVINDQIGAPTGADLIADVTAHAIRQVLQRPDLAGVYHLAAAGEVSWHGYAQHVVEVAKAHGE
TLAVMSINPIETAAYSTPARRPRNSRLNTRKLRDNFSLHLPDWQSGVTRMLMEVLNK

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=237, Percent_Identity=28.2700421940928, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI33519455, Length=237, Percent_Identity=28.2700421940928, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1788352, Length=295, Percent_Identity=60, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32480; Mature: 32480

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLLGKNGQVGWELQRSLAPLGELLSLDRQTVGGLCGDLTDLEGLRATIRTVRPDVIV
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEE
NAAAYTAVDKAESERELVDLVNVKAVQALAEEALSLGAWLVHYSTDYVFSGEGTTPWLED
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCCCCCC
AAVAPVNHYGASKFAGEEAVVASGCKYLIFRPSWIYSARGNNFAKTMLRLAREREELNVI
CCCCCHHHCCCHHHCCCHHHHHCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHH
NDQIGAPTGADLIADVTAHAIRQVLQRPDLAGVYHLAAAGEVSWHGYAQHVVEVAKAHGE
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
TLAVMSINPIETAAYSTPARRPRNSRLNTRKLRDNFSLHLPDWQSGVTRMLMEVLNK
EEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHHCCCCEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRILLLGKNGQVGWELQRSLAPLGELLSLDRQTVGGLCGDLTDLEGLRATIRTVRPDVIV
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEE
NAAAYTAVDKAESERELVDLVNVKAVQALAEEALSLGAWLVHYSTDYVFSGEGTTPWLED
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCCCCCC
AAVAPVNHYGASKFAGEEAVVASGCKYLIFRPSWIYSARGNNFAKTMLRLAREREELNVI
CCCCCHHHCCCHHHCCCHHHHHCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHH
NDQIGAPTGADLIADVTAHAIRQVLQRPDLAGVYHLAAAGEVSWHGYAQHVVEVAKAHGE
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
TLAVMSINPIETAAYSTPARRPRNSRLNTRKLRDNFSLHLPDWQSGVTRMLMEVLNK
EEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHHCCCCEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]