BacMap

Definition	Pseudomonas fluorescens Pf0-1 chromosome, complete genome.
Accession	NC_007492
Length	6,438,405

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The map label for this gene is fabG [C]

Identifier: 77460261

GI number: 77460261

Start: 4565842

End: 4566636

Strand: Reverse

Name: fabG [C]

Synonym: Pfl01_4040

Alternate gene names: 77460261

Gene position: 4566636-4565842 (Counterclockwise)

Preceding gene: 77460262

Following gene: 77460260

Centisome position: 70.93

GC content: 61.76

Gene sequence:

>795_bases
ATGAATTCCCCGAAATCCCAAGGCACGGCGCTGGTCACCGGCGCTTCTTCCGGCATCGGTGCGGTGTATGCCGAGCGATT
GGCGGCACGTGGTTTTGACCTGTTGCTGGTGGCTCGCGATCAGGAGCGTCTGGAGTCCGCGGCCAGTAAGCTGCGAGACG
CACATGGCGTTCAGGTCGAAGTGCTGAAGGCCGACCTGACGCAAAAGGGTGAAGTGCTCAAGCTTGAACAACGTCTGCGC
AGCGATTCCAGTATCAGCCTGCTGGTCAACAATGCGGGCGTAGCGGTCAATGGCTTGTTGGCTAATGCCGACCCCGAGAC
CCTGGAGCAACTGATCCAGTTGAACGTCACCACGCTGACCCGACTGGCAGCCGCAGCCGCCTCGGCGTTCACCCGCGCCG
GGCGCGGCACGATCATCAATATCGCGTCCGTGGTCGCACTGTTCCCCGAACGCTTCAACGCCACCTACAGCGCCAGCAAG
GCTTACGTGTTGAGTCTGACTCAATCGCTCAATACCGAACTCGAAGGCACCGGGGTCAAGGTGCAAGCGGTATTGCCCGG
TGTGACCCGCACTGAAATCTGGGAGCGTTCCGGATTCGATGCCAGCGGTATCCCTGCGGAGATGGTCATGGAGGCGGGCG
AGATGGTCGATGCCGCGCTGTCCGGTCTGGATCAGGGCGAACTGGTCACGATTCCTTCGCTGCCCGATGCGGGCGAGTGG
CAAGCCTTTGTCGCCGCCCGCCATGTAATGGCGCCCAACCTGTCCCACAGTTCGGCGGCCAGCCGCTACAAGTAA

Upstream 100 bases:

>100_bases
TCATATTAGTGTGAGATTATGCCTATAATAATTTCAGTCCCCCTGAAGCCCTCTGCTTTTCAGCGGTCGCCCATTCCTTA
CGCATTCCAGGAGTATCGTC

Downstream 100 bases:

>100_bases
AAGACTTTTGAGTCGCGAGAGGTCAACCCGATATGGATCAGCGTCGAGTAGTTGTCACGGGTATGGGCCTGGTATCGCCC
TTGGGAAGCGGCGTCGAAGT

Product: Short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNSPKSQGTALVTGASSGIGAVYAERLAARGFDLLLVARDQERLESAASKLRDAHGVQVEVLKADLTQKGEVLKLEQRLR
SDSSISLLVNNAGVAVNGLLANADPETLEQLIQLNVTTLTRLAAAAASAFTRAGRGTIINIASVVALFPERFNATYSASK
AYVLSLTQSLNTELEGTGVKVQAVLPGVTRTEIWERSGFDASGIPAEMVMEAGEMVDAALSGLDQGELVTIPSLPDAGEW
QAFVAARHVMAPNLSHSSAASRYK

Sequences:

>Translated_264_residues
MNSPKSQGTALVTGASSGIGAVYAERLAARGFDLLLVARDQERLESAASKLRDAHGVQVEVLKADLTQKGEVLKLEQRLR
SDSSISLLVNNAGVAVNGLLANADPETLEQLIQLNVTTLTRLAAAAASAFTRAGRGTIINIASVVALFPERFNATYSASK
AYVLSLTQSLNTELEGTGVKVQAVLPGVTRTEIWERSGFDASGIPAEMVMEAGEMVDAALSGLDQGELVTIPSLPDAGEW
QAFVAARHVMAPNLSHSSAASRYK
>Mature_264_residues
MNSPKSQGTALVTGASSGIGAVYAERLAARGFDLLLVARDQERLESAASKLRDAHGVQVEVLKADLTQKGEVLKLEQRLR
SDSSISLLVNNAGVAVNGLLANADPETLEQLIQLNVTTLTRLAAAAASAFTRAGRGTIINIASVVALFPERFNATYSASK
AYVLSLTQSLNTELEGTGVKVQAVLPGVTRTEIWERSGFDASGIPAEMVMEAGEMVDAALSGLDQGELVTIPSLPDAGEW
QAFVAARHVMAPNLSHSSAASRYK

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG0300

COG function: function code R; Short-chain dehydrogenases of various substrate specificities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4557649, Length=185, Percent_Identity=32.972972972973, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI226371731, Length=193, Percent_Identity=29.5336787564767, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI7705855, Length=188, Percent_Identity=28.1914893617021, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI225579078, Length=222, Percent_Identity=29.7297297297297, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI142976729, Length=232, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI40254992, Length=185, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI19923817, Length=215, Percent_Identity=27.4418604651163, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI15277342, Length=219, Percent_Identity=30.1369863013699, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI109715829, Length=192, Percent_Identity=29.6875, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI186928839, Length=197, Percent_Identity=30.9644670050761, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI150247226, Length=207, Percent_Identity=29.951690821256, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI119392083, Length=232, Percent_Identity=28.8793103448276, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI32483357, Length=195, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20149619, Length=189, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=27.9187817258883, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI5031737, Length=190, Percent_Identity=27.3684210526316, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI7706318, Length=191, Percent_Identity=28.7958115183246, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787335, Length=185, Percent_Identity=32.972972972973, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787820, Length=180, Percent_Identity=31.1111111111111, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87082160, Length=191, Percent_Identity=28.2722513089005, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788459, Length=199, Percent_Identity=30.6532663316583, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790717, Length=184, Percent_Identity=27.7173913043478, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082100, Length=189, Percent_Identity=29.1005291005291, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786701, Length=206, Percent_Identity=28.6407766990291, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367175, Length=198, Percent_Identity=30.8080808080808, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI212646214, Length=197, Percent_Identity=30.4568527918782, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17557668, Length=197, Percent_Identity=31.9796954314721, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=194, Percent_Identity=31.4432989690722, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=199, Percent_Identity=31.6582914572864, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17559552, Length=191, Percent_Identity=29.8429319371728, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25152911, Length=195, Percent_Identity=31.2820512820513, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=212, Percent_Identity=31.6037735849057, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=240, Percent_Identity=29.5833333333333, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=190, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=195, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=227, Percent_Identity=28.6343612334802, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=189, Percent_Identity=28.042328042328, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=199, Percent_Identity=30.1507537688442, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25147288, Length=218, Percent_Identity=28.4403669724771, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560176, Length=191, Percent_Identity=28.7958115183246, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=207, Percent_Identity=31.4009661835749, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=197, Percent_Identity=26.9035532994924, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=212, Percent_Identity=28.3018867924528, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=202, Percent_Identity=26.7326732673267, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=217, Percent_Identity=29.0322580645161, Blast_Score=64, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17532725, Length=186, Percent_Identity=25.2688172043011, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6319635, Length=186, Percent_Identity=30.6451612903226, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323882, Length=197, Percent_Identity=32.48730964467, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322067, Length=195, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320089, Length=197, Percent_Identity=31.9796954314721, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640442, Length=189, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28571526, Length=272, Percent_Identity=34.5588235294118, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24584752, Length=189, Percent_Identity=35.4497354497354, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI23397609, Length=194, Percent_Identity=32.4742268041237, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357041, Length=262, Percent_Identity=31.6793893129771, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24584748, Length=192, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161076933, Length=192, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644339, Length=262, Percent_Identity=29.0076335877863, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24649181, Length=196, Percent_Identity=27.5510204081633, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24584744, Length=192, Percent_Identity=27.6041666666667, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574402, Length=192, Percent_Identity=27.6041666666667, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI116007236, Length=209, Percent_Identity=28.2296650717703, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571387, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355319, Length=187, Percent_Identity=27.807486631016, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24639444, Length=217, Percent_Identity=29.4930875576037, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665247, Length=191, Percent_Identity=31.413612565445, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI221331216, Length=191, Percent_Identity=31.413612565445, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21358495, Length=192, Percent_Identity=29.1666666666667, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24643142, Length=216, Percent_Identity=30.0925925925926, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24641388, Length=222, Percent_Identity=28.3783783783784, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24641232, Length=197, Percent_Identity=28.9340101522843, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 27776; Mature: 27776

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPKSQGTALVTGASSGIGAVYAERLAARGFDLLLVARDQERLESAASKLRDAHGVQVE
CCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEE
VLKADLTQKGEVLKLEQRLRSDSSISLLVNNAGVAVNGLLANADPETLEQLIQLNVTTLT
EEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHCCHHHHH
RLAAAAASAFTRAGRGTIINIASVVALFPERFNATYSASKAYVLSLTQSLNTELEGTGVK
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHEEEEHHHHCCCCCCCCEE
VQAVLPGVTRTEIWERSGFDASGIPAEMVMEAGEMVDAALSGLDQGELVTIPSLPDAGEW
EEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCH
QAFVAARHVMAPNLSHSSAASRYK
HHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure
MNSPKSQGTALVTGASSGIGAVYAERLAARGFDLLLVARDQERLESAASKLRDAHGVQVE
CCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEE
VLKADLTQKGEVLKLEQRLRSDSSISLLVNNAGVAVNGLLANADPETLEQLIQLNVTTLT
EEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHCCHHHHH
RLAAAAASAFTRAGRGTIINIASVVALFPERFNATYSASKAYVLSLTQSLNTELEGTGVK
HHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCHHEEEEHHHHCCCCCCCCEE
VQAVLPGVTRTEIWERSGFDASGIPAEMVMEAGEMVDAALSGLDQGELVTIPSLPDAGEW
EEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCH
QAFVAARHVMAPNLSHSSAASRYK
HHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA