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The map label for this gene is greB [H]

Identifier: 77460238

GI number: 77460238

Start: 4542639

End: 4543136

Strand: Direct

Name: greB [H]

Synonym: Pfl01_4017

Alternate gene names: 77460238

Gene position: 4542639-4543136 (Clockwise)

Preceding gene: 77460237

Following gene: 77460242

Centisome position: 70.56

GC content: 61.24

Gene sequence:

>498_bases
ATGAGCCGTTATCGCCCTCCCCGCACCGCCGGCACTGCGCTGATCACCCCTGAAGGTGAAGCGCGGATGCGCGCCGAACT
CCATGAGCTGTGGCACGTGCGCCGCCCGCAGGTGACGCAGTCAGTCAGCGAGGCGGCGGCTCAGGGTGATCGTTCGGAAA
ATGCCGAGTACACCTACGGCAAGAAAATGCTGCGCGAGATCGACAGCCGCGTGCGCTTTCTCACCAAACGCCTTGAGGCG
TCGAAAGTGGTCAGCGAAAAACCCAGTGATCCGAACAAGGTCTATTTCGGTGCCTGGGTGACCATCGAAGACGAGGACGG
CAAACAGTCGCGCTATCGTATCGTCGGCCCGGACGAACTGGATCTGAAGCTCGGCCTGATCAGCATCGACTCACCGCTGG
CCCGCGCCCTTATCGGCAAGGCGCTGGATGCAGAAGTTCGGGTACAGACGCCGACCGGCGAACAGTTCGTCTACATCGTG
GCGATCGAATATCCCTGA

Upstream 100 bases:

>100_bases
GCCGGGGTATTCGGCACACGGCGTGCGCTGAACGCCAGCCCGCTGACAGTGTTGCGCGAGGGTTGATAGACTCCAGCCGT
CTTGATCAAAAGAAGTTGCC

Downstream 100 bases:

>100_bases
CGCTCAGCGCCGGGTGATCAGGCCCTGGCGTGCGACGCGGGTCAGTTGGCGAATCATTTCCGGCGCCTCTTCACGGCTCG
GGGCCTGAATGACGGCCAGA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MSRYRPPRTAGTALITPEGEARMRAELHELWHVRRPQVTQSVSEAAAQGDRSENAEYTYGKKMLREIDSRVRFLTKRLEA
SKVVSEKPSDPNKVYFGAWVTIEDEDGKQSRYRIVGPDELDLKLGLISIDSPLARALIGKALDAEVRVQTPTGEQFVYIV
AIEYP

Sequences:

>Translated_165_residues
MSRYRPPRTAGTALITPEGEARMRAELHELWHVRRPQVTQSVSEAAAQGDRSENAEYTYGKKMLREIDSRVRFLTKRLEA
SKVVSEKPSDPNKVYFGAWVTIEDEDGKQSRYRIVGPDELDLKLGLISIDSPLARALIGKALDAEVRVQTPTGEQFVYIV
AIEYP
>Mature_164_residues
SRYRPPRTAGTALITPEGEARMRAELHELWHVRRPQVTQSVSEAAAQGDRSENAEYTYGKKMLREIDSRVRFLTKRLEAS
KVVSEKPSDPNKVYFGAWVTIEDEDGKQSRYRIVGPDELDLKLGLISIDSPLARALIGKALDAEVRVQTPTGEQFVYIVA
IEYP

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=53.5947712418301, Blast_Score=158, Evalue=2e-40,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=38.1578947368421, Blast_Score=102, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18647; Mature: 18516

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYRPPRTAGTALITPEGEARMRAELHELWHVRRPQVTQSVSEAAAQGDRSENAEYTYG
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHH
KKMLREIDSRVRFLTKRLEASKVVSEKPSDPNKVYFGAWVTIEDEDGKQSRYRIVGPDEL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCEEEECCCCC
DLKLGLISIDSPLARALIGKALDAEVRVQTPTGEQFVYIVAIEYP
CEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEECC
>Mature Secondary Structure 
SRYRPPRTAGTALITPEGEARMRAELHELWHVRRPQVTQSVSEAAAQGDRSENAEYTYG
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHH
KKMLREIDSRVRFLTKRLEASKVVSEKPSDPNKVYFGAWVTIEDEDGKQSRYRIVGPDEL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCEEEECCCCC
DLKLGLISIDSPLARALIGKALDAEVRVQTPTGEQFVYIVAIEYP
CEEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]