Click here to switch to the map view.

The map label for this gene is nreB [H]

Identifier: 77460177

GI number: 77460177

Start: 4483747

End: 4484718

Strand: Direct

Name: nreB [H]

Synonym: Pfl01_3956

Alternate gene names: 77460177

Gene position: 4483747-4484718 (Clockwise)

Preceding gene: 77460176

Following gene: 77460178

Centisome position: 69.64

GC content: 59.88

Gene sequence:

>972_bases
ATGACAGTTTTCCAGACAGAGACCGCCAGCCCTTCTGACCGGTCTGCCCGCAGCGAACATCCATTGGCTCCATCCGCTAT
GTACGCCAGCCTCAAGTCAATCACCACATGGCCTCCCTCCCGAGAAAATGCCCGCAGGTTCACACTTCTGCTGTGTACAG
GCTCGGCTCTGGGCAGTCTGTTGACCTATGGATTGTCAGCGCAGCTGCCGCTCGGCCTGCTGCTGATCAATGTGGCGGCT
GCGGGCTGCGTCTGGACACAACATCGCCTGTCGCGCAAATCCATCAAGTTCCAGCCTCAGGAACTGGCCGACCGTTTGCT
GGAAGTCCAGGAAAACGAGCGCCATCGCCTGAGCCGTGAACTGCATGACGATATCGGCCAGTTGCTGACGGCCGCCAAGC
TGCAAAGCGAATGGCTCAAGCGTCGCTTGCCCGCGGATATGCAGGAACACTGTTCGGTCCTTTGCGAGACTCTCGAAGAA
ACCCTGAACAAGGTTCGCGACGTTTCGGCCATTCTCAACCCGCGGCAACTGACCAGCCTTGGTCTGGAAGCGAGCTTGCG
TGCGCACCTGCTCAAGGTACTGACCAATACTTCGGTGCACTGGAGCCTTGACTGCCAGCAGCGCCTGAACGGAATCCCCG
AAGAAATGGCCGTCGCCGCCTTTCGCATCACTCAGGAAGCGGTGACCAACATCCTTCGCCACGCGCAGGCCAAAAACCTG
TTGATCCGCGTGCAACGTTTACCGCAGGGCCTGACCCTGCTGATCAGCGATGATGGACTGGGCTTTTCCCCGGCGGCGAA
TCCGGGTCGCGAAGGACAGCGAGGCATGGCCGGAATGGCCGAACGGATCGAGCAACTGGGCGGCACACTGAGCGTGACCA
GCGAGCCGGGCAAAGGCACTCAAATCGAAGCACTATTCCCCTGGGCGCCCCGTGCACTCGAGCGGGCCAGTACGAATAAG
GTTATGCGTTGA

Upstream 100 bases:

>100_bases
TAGTTGTTTCAGTTAATGGCATTGTGACTTCCATGAGTGATCCCACCGAAGTTGGCGCTCAATTATCGATCACTGAACCA
TTTTGAAATCAACAACCTGA

Downstream 100 bases:

>100_bases
CTTGTAACTTACTTCTGGTGGATGACCACTCGCTGATCAGGGCCGGCGTGCGCGCCCTGGTGCTGGATATTCCCGGTTAT
GCGGTCATCGGCGAGGCCAA

Product: histidine kinase, dimerization and phosphoacceptor region

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTVFQTETASPSDRSARSEHPLAPSAMYASLKSITTWPPSRENARRFTLLLCTGSALGSLLTYGLSAQLPLGLLLINVAA
AGCVWTQHRLSRKSIKFQPQELADRLLEVQENERHRLSRELHDDIGQLLTAAKLQSEWLKRRLPADMQEHCSVLCETLEE
TLNKVRDVSAILNPRQLTSLGLEASLRAHLLKVLTNTSVHWSLDCQQRLNGIPEEMAVAAFRITQEAVTNILRHAQAKNL
LIRVQRLPQGLTLLISDDGLGFSPAANPGREGQRGMAGMAERIEQLGGTLSVTSEPGKGTQIEALFPWAPRALERASTNK
VMR

Sequences:

>Translated_323_residues
MTVFQTETASPSDRSARSEHPLAPSAMYASLKSITTWPPSRENARRFTLLLCTGSALGSLLTYGLSAQLPLGLLLINVAA
AGCVWTQHRLSRKSIKFQPQELADRLLEVQENERHRLSRELHDDIGQLLTAAKLQSEWLKRRLPADMQEHCSVLCETLEE
TLNKVRDVSAILNPRQLTSLGLEASLRAHLLKVLTNTSVHWSLDCQQRLNGIPEEMAVAAFRITQEAVTNILRHAQAKNL
LIRVQRLPQGLTLLISDDGLGFSPAANPGREGQRGMAGMAERIEQLGGTLSVTSEPGKGTQIEALFPWAPRALERASTNK
VMR
>Mature_322_residues
TVFQTETASPSDRSARSEHPLAPSAMYASLKSITTWPPSRENARRFTLLLCTGSALGSLLTYGLSAQLPLGLLLINVAAA
GCVWTQHRLSRKSIKFQPQELADRLLEVQENERHRLSRELHDDIGQLLTAAKLQSEWLKRRLPADMQEHCSVLCETLEET
LNKVRDVSAILNPRQLTSLGLEASLRAHLLKVLTNTSVHWSLDCQQRLNGIPEEMAVAAFRITQEAVTNILRHAQAKNLL
IRVQRLPQGLTLLISDDGLGFSPAANPGREGQRGMAGMAERIEQLGGTLSVTSEPGKGTQIEALFPWAPRALERASTNKV
MR

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4564

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=197, Percent_Identity=31.9796954314721, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082311, Length=212, Percent_Identity=35.377358490566, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787474, Length=197, Percent_Identity=28.4263959390863, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 35689; Mature: 35557

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVFQTETASPSDRSARSEHPLAPSAMYASLKSITTWPPSRENARRFTLLLCTGSALGSL
CCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHH
LTYGLSAQLPLGLLLINVAAAGCVWTQHRLSRKSIKFQPQELADRLLEVQENERHRLSRE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
LHDDIGQLLTAAKLQSEWLKRRLPADMQEHCSVLCETLEETLNKVRDVSAILNPRQLTSL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GLEASLRAHLLKVLTNTSVHWSLDCQQRLNGIPEEMAVAAFRITQEAVTNILRHAQAKNL
HHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIRVQRLPQGLTLLISDDGLGFSPAANPGREGQRGMAGMAERIEQLGGTLSVTSEPGKGT
HHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCC
QIEALFPWAPRALERASTNKVMR
EEEEECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVFQTETASPSDRSARSEHPLAPSAMYASLKSITTWPPSRENARRFTLLLCTGSALGSL
CEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHH
LTYGLSAQLPLGLLLINVAAAGCVWTQHRLSRKSIKFQPQELADRLLEVQENERHRLSRE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHH
LHDDIGQLLTAAKLQSEWLKRRLPADMQEHCSVLCETLEETLNKVRDVSAILNPRQLTSL
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
GLEASLRAHLLKVLTNTSVHWSLDCQQRLNGIPEEMAVAAFRITQEAVTNILRHAQAKNL
HHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIRVQRLPQGLTLLISDDGLGFSPAANPGREGQRGMAGMAERIEQLGGTLSVTSEPGKGT
HHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCC
QIEALFPWAPRALERASTNKVMR
EEEEECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA