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The map label for this gene is iscS [H]

Identifier: 77460133

GI number: 77460133

Start: 4429628

End: 4430794

Strand: Reverse

Name: iscS [H]

Synonym: Pfl01_3912

Alternate gene names: 77460133

Gene position: 4430794-4429628 (Counterclockwise)

Preceding gene: 77460134

Following gene: 77460132

Centisome position: 68.82

GC content: 62.9

Gene sequence:

>1167_bases
ATGAACACACGTCCGCTGTATTTCGATTACGCCGCCACCACCCCGGTGGACGAGCGGGTCATCCAGGTGATGATCGAGTG
TCTGGGTTTCAACGGTAACTTCGGCAACCCGGCCTCCAGTTCCCACGCTTTCGGCCAGCAGGCCCGGCAAACGGTCGAGA
ACGCTCGCCGGCAAGTCGCCGAACTGGTCGGCGCCCAGGCGCAGCAGATCGTCTGGACCTCCGGCGCCACCGAATCCAAC
AACCTCGCCCTCAAAGGCGTGGCCCAGGCCCGTGGTGTGTCCGGCGGCCACATCATCACCAGTCAGATCGAACACAAGGC
CATCCTCGATACCGCCCGGCAATTGCAGGACGCCGGTGTCGCCGTGACCTATCTGGTGCCGGACGCCGAAGGCCTGATCA
CTGCGCAAGCGGTCAGCGAAGCGATGCGCGATGACACCTTTCTGGTGTCGCTGATGCTGGTCAACAACGAACTCGGCACC
GTCAACGACATCCCGGCCATCGGCGAAGTAGTGCGTGGGCGCGGGGCGTTGTTCCATGTCGATGCGGCGCAAGGCGCCGG
CAAGGTCGCGATCGATCTGGCGCAATGGCCGGTGGATCTCATGTCGTTTTCGGCACACAAGCTCTACGGCCCCAAAGGCA
TCGGCGCGCTGTATGTCGGACCCCATGCGCAACAGCGCTTGCAGGCGCAGATTCACGGTGGTGGTCACGAGGGTGGGTTG
CGTTCCGGTACGCTGGCCACTCACCAGATCGCCGCCATGGGCTCGGCCTTTGCCTTGGCCGCCGAGGCCTTTGATGACGA
GAAGAAAACCATCGTCGCGTTGCGCGAGCGACTGCTCGAACAACTCTTGAGCCTGCCCGGCGTACGCCTCAACGGCAGCT
CCACCCATCGTATCCCGCACACCTTGAGCCTGACCTTCAGCGAAGGCGAGTTCAACCCGGCGGCGCTGAGCCATTCGATC
GCGTTTTCCGCGACTTCGGCCTGCAATTCCGCCAGCAATACACCGTCCCACGTGCTGCTGGCATTGGGGCACGATGCGCA
TTTGGCCGGCCGTACCATTCGTTTGAGCCTCGGCCGTTTCACCACCGCCGAAGACATCGACAAGGCTGTAGATCTGATCA
AGACCGCCTGCGCCAGTGCTACGGCATTCTGGGCGACAGGGCTTTAA

Upstream 100 bases:

>100_bases
TTGATGACGCTCTCGAACCCGGACGCAGCTCACAAGGCTGCGTCGTGCCGTTTGCAGACACGAGCATCCTGATCCCGGAA
CACGCTCTGCGAGTCTTTTT

Downstream 100 bases:

>100_bases
CGAGCCGAATTCGAACGGCACCAAACAATAACGATGAAGTGATTCGCAGGAGACATGATGAGTACCCAGACCTTTACCGA
AGGAACGGTTCCCCAGCGCC

Product: aromatic amino acid beta-eliminating lyase/threonine aldolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNTRPLYFDYAATTPVDERVIQVMIECLGFNGNFGNPASSSHAFGQQARQTVENARRQVAELVGAQAQQIVWTSGATESN
NLALKGVAQARGVSGGHIITSQIEHKAILDTARQLQDAGVAVTYLVPDAEGLITAQAVSEAMRDDTFLVSLMLVNNELGT
VNDIPAIGEVVRGRGALFHVDAAQGAGKVAIDLAQWPVDLMSFSAHKLYGPKGIGALYVGPHAQQRLQAQIHGGGHEGGL
RSGTLATHQIAAMGSAFALAAEAFDDEKKTIVALRERLLEQLLSLPGVRLNGSSTHRIPHTLSLTFSEGEFNPAALSHSI
AFSATSACNSASNTPSHVLLALGHDAHLAGRTIRLSLGRFTTAEDIDKAVDLIKTACASATAFWATGL

Sequences:

>Translated_388_residues
MNTRPLYFDYAATTPVDERVIQVMIECLGFNGNFGNPASSSHAFGQQARQTVENARRQVAELVGAQAQQIVWTSGATESN
NLALKGVAQARGVSGGHIITSQIEHKAILDTARQLQDAGVAVTYLVPDAEGLITAQAVSEAMRDDTFLVSLMLVNNELGT
VNDIPAIGEVVRGRGALFHVDAAQGAGKVAIDLAQWPVDLMSFSAHKLYGPKGIGALYVGPHAQQRLQAQIHGGGHEGGL
RSGTLATHQIAAMGSAFALAAEAFDDEKKTIVALRERLLEQLLSLPGVRLNGSSTHRIPHTLSLTFSEGEFNPAALSHSI
AFSATSACNSASNTPSHVLLALGHDAHLAGRTIRLSLGRFTTAEDIDKAVDLIKTACASATAFWATGL
>Mature_388_residues
MNTRPLYFDYAATTPVDERVIQVMIECLGFNGNFGNPASSSHAFGQQARQTVENARRQVAELVGAQAQQIVWTSGATESN
NLALKGVAQARGVSGGHIITSQIEHKAILDTARQLQDAGVAVTYLVPDAEGLITAQAVSEAMRDDTFLVSLMLVNNELGT
VNDIPAIGEVVRGRGALFHVDAAQGAGKVAIDLAQWPVDLMSFSAHKLYGPKGIGALYVGPHAQQRLQAQIHGGGHEGGL
RSGTLATHQIAAMGSAFALAAEAFDDEKKTIVALRERLLEQLLSLPGVRLNGSSTHRIPHTLSLTFSEGEFNPAALSHSI
AFSATSACNSASNTPSHVLLALGHDAHLAGRTIRLSLGRFTTAEDIDKAVDLIKTACASATAFWATGL

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=371, Percent_Identity=44.743935309973, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=32.8502415458937, Blast_Score=179, Evalue=3e-45,
Organism=Escherichia coli, GI48994898, Length=374, Percent_Identity=53.7433155080214, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1789175, Length=215, Percent_Identity=33.4883720930233, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787970, Length=311, Percent_Identity=27.6527331189711, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=377, Percent_Identity=46.4190981432361, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17533177, Length=334, Percent_Identity=31.437125748503, Blast_Score=154, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6319831, Length=373, Percent_Identity=45.5764075067024, Blast_Score=325, Evalue=6e-90,
Organism=Drosophila melanogaster, GI20129463, Length=371, Percent_Identity=46.6307277628032, Blast_Score=314, Evalue=8e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40915; Mature: 40915

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRPLYFDYAATTPVDERVIQVMIECLGFNGNFGNPASSSHAFGQQARQTVENARRQVA
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELVGAQAQQIVWTSGATESNNLALKGVAQARGVSGGHIITSQIEHKAILDTARQLQDAGV
HHHCCCHHEEEEECCCCCCCCEEEEEHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCE
AVTYLVPDAEGLITAQAVSEAMRDDTFLVSLMLVNNELGTVNDIPAIGEVVRGRGALFHV
EEEEEECCCCCCEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEE
DAAQGAGKVAIDLAQWPVDLMSFSAHKLYGPKGIGALYVGPHAQQRLQAQIHGGGHEGGL
ECCCCCCEEEEEHHHCCHHHHHCCHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCC
RSGTLATHQIAAMGSAFALAAEAFDDEKKTIVALRERLLEQLLSLPGVRLNGSSTHRIPH
CCCCCHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCE
TLSLTFSEGEFNPAALSHSIAFSATSACNSASNTPSHVLLALGHDAHLAGRTIRLSLGRF
EEEEEEECCCCCHHHHHHHHEEEHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
TTAEDIDKAVDLIKTACASATAFWATGL
CCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTRPLYFDYAATTPVDERVIQVMIECLGFNGNFGNPASSSHAFGQQARQTVENARRQVA
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELVGAQAQQIVWTSGATESNNLALKGVAQARGVSGGHIITSQIEHKAILDTARQLQDAGV
HHHCCCHHEEEEECCCCCCCCEEEEEHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCE
AVTYLVPDAEGLITAQAVSEAMRDDTFLVSLMLVNNELGTVNDIPAIGEVVRGRGALFHV
EEEEEECCCCCCEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEE
DAAQGAGKVAIDLAQWPVDLMSFSAHKLYGPKGIGALYVGPHAQQRLQAQIHGGGHEGGL
ECCCCCCEEEEEHHHCCHHHHHCCHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCC
RSGTLATHQIAAMGSAFALAAEAFDDEKKTIVALRERLLEQLLSLPGVRLNGSSTHRIPH
CCCCCHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCE
TLSLTFSEGEFNPAALSHSIAFSATSACNSASNTPSHVLLALGHDAHLAGRTIRLSLGRF
EEEEEEECCCCCHHHHHHHHEEEHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
TTAEDIDKAVDLIKTACASATAFWATGL
CCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA